• 한국미생물·생명공학회지
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• 제51권4호
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• pp.500-506
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• 2023

Carbapenem 내성 Klebsiella pneumoniae (Carbapenem-resistant K. pneumonia, CRKP)는 전 세계적인 공중 보건 문제로 대두되고 있다. 최근 Klebsiella pneumoniae carbapenemase-2 (KPC-2) 생성 sequence type (ST)307은 CRKP의 주요 클론으로 확인되었으며, 국내에서 ST307의 확산이 보고되었다. 본 연구에서는 2020년 3월부터 2021년 12월까지 대전지역의 3차 병원에서 분리된 CRKP 50균주를 대상으로, 분자 특성과 항균제 내성 양상을 조사하였다. 역학적 관계는 multilocus sequence typing (MLST)를 통해 분석하였고, 항균제 감수성 검사는 디스크 확산법을 통해 확인하였다. PCR과 DNA 염기서열분석은 carbapenemase 유전자 확인을 위해 수행하였다. CRKP감염은 남성과 60세 이상의 환자에서 훨씬 더 빈번하게 확인되었다. CRKP 50균주 중 46균주(92.0%)는 다제내성(MDR)을 보였고, 44균주(88.0%)는 carbapenemase-producing K. pneumoniae (CPKP)로 확인되었다. 주요 carbapenemase 유형은 KPC-2 (36균주, 72.0%)였으며, New Delhi metallo-enzyme-1 (NDM-1)과 NDM-5는 각각 7균주(14.0%)와 1균주(2.0%)에서 확인되었다. 특히, KPC-2 생성 K. pneumoniae의 88.9% (32/36)가 ST307에 속한 반면, NDM-1,-5 생성 K. pneumoniae의 87.5% (7/8)가 non-ST307에 속한 것을 확인하였다. 이러한 결과는 ST307의 확산 뿐만 아니라 non-ST307의 발달을 예방하기 위한 적절한 감염관리와 효과적인 감시체계가 필요하다고 사료된다.

• 대한임상검사과학회지
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• 제54권4호
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• pp.265-272
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• 2022

Carbapenemase 생성 장내 세균(carbapenemase-producing Enterobacteriaceae, CPE) 중 특히 KPC-2 생성 Klebsiella pneumoniae의 출현과 확산은 전 세계적으로 급격히 증가하고 있으며, 심각한 공중 보건 위협이 되고 있다. 이러한 KPC-2 생성 K. pneumoniae의 역학과 특징은 지역 및 기간에 따라 다르기 때문에, 본 연구에서는 2017년 3월부터 2020년 12월까지 대전지역의 3차 병원에서 분리된 carbapenem 내성 K. pneumoniae (carbapenem-resistant K. pneumoniae, CRKP) 78 균주를 대상으로 carbapenemase 유전자를 분석하고, 이에 대한 역학 관계를 조사하였다. 항균제 감수성 양상은 디스크 확산법으로 확인하였고, carbapenemase 유전자의 분석을 위해 PCR과 DNA 염기서열분석을 수행하였다. 또한, 역학관계는 MLST (multilocus sequence typing)를 통해 조사하였다. 78 균주의 CRKP 중 35 균주(44.9%)가 carbapenemase 생성 K. pneumoniae였으며, 주요 carbapenemase 유형은 KPC-2 (30주, 85.7%)로 확인되었다. NDM-1과 NDM-5는 각각 4 균주(11.4%)와 1 균주(2.9%)에서 확인되었다. MLST 분석에서 10개의 sequence type (ST)이 확인되었고, 가장 흔한 ST는 ST307 (51.4%, 18/35)이었다. ST307 균주는 모두 KPC-2 생성 K. pneumonia였고, 다제내성으로 확인되었다. 또한, ST307은 4년 동안 지속적으로 출현하였다. 이러한 결과는 KPC-2 생성 K. pneumoniae ST307의 확산을 방지하기 위해 지속적인 모니터링과 적절한 감염 관리가 필요할 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.974-981
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• 2020

Sequence type 410 (ST410) of Escherichia coli is an extraintestinal pathogen associated with multi drug resistance. In this study, we aimed to investigate the horizontal propagation pathway of a high-risk clone of E. coli ST410 that produces Klebsiella pneumoniae carbapenemase (KPC). blaKPC-encoding E. coli and K. pneumoniae isolates were evaluated, and complete sequencing and comparative analysis of blaKPC-encoding plasmids from E. coli and K. pneumoniae, antimicrobial susceptibility tests, polymerase chain reaction, multilocus sequence typing, and conjugal transfer of plasmids were performed. Whole-genome sequencing was performed for plasmids mediating KPC-2 production in E. coli and K. pneumoniae clinical isolates. Strains E. coli CPEc171209 and K. pneumoniae CPKp171210 were identified as ST410 and ST307, respectively. CPEc171209 harbored five plasmids belonging to serotype O8:H21, which is in the antimicrobial-resistant clade C4/H24. The CPKp171210 isolate harbored three plasmids. Both strains harbored various additional antimicrobial resistance genes. The IncX3 plasmid pECBHS_9_5 harbored blaKPC-2 within a truncated Tn4401a transposon, which also contains blaSHV-182 with duplicated conjugative elements. This plasmid displayed 100% identity with the IncX3 plasmid pKPBHS_10_3 from the K. pneumoniae CPKp171210 ST307 strain. The genes responsible for the conjugal transfer of the IncX3 plasmid included tra/trb clusters and pil genes coding the type IV pilus. ST410 can be transmitted between patients, posing an elevated risk in clinical settings. The emergence of a KPC-producing E. coli strain (ST410) is concerning because the blaKPC-2-bearing plasmids may carry treatment resistance across species barriers. Transgenic translocation occurs among carbapenem-resistant bacteria, which may spread rapidly via horizontal migration.

• 식물분류학회지
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• 제42권4호
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• pp.307-315
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• 2012

한국산 나비나물속(Vicia)에 속하는 10분류군의 체세포 염색체수와 그 중 6분류군에 대한 핵형분석을 실시하였다. 본 연구에서 밝혀진 나비나물속의 체세포 염색체수는 2n = 12, 14 또는 24로서, 기본 염색체수 x = 6 또는 7의 이배체와 사배체로 밝혀졌다. 한국특산식물인 나래완두(V. hirticalycina, 2n = 2x = 12)의 염색체수는 본 연구에서 처음으로 밝혀졌다. 가는살갈퀴(V. angustifolia, 2n = 2x = 12), 등갈퀴나물(V. cracca, 2n = 4x = 24), 새완두(V. hirsuta, 2n = 2x = 14), 얼치기완두(V. tetrasperma, 2n = 2x = 14 + 2B), 벌완두(V. amurensis, 2n = 2x = 12), 노랑갈퀴(V. chosenensis, 2n = 2x = 12, 12 + 2B), 나비나물(V. unijuga, 2n = 4x = 24), 애기나비나물(V. unijuga f. minor, 2n = 4x = 24), 광릉갈퀴(V. venosa var. cuspidata, 2n = 4x = 24)의 체세포 염색체수는 기존의 보고들과 일치하였다. 핵형분석 결과, 등갈퀴나물은 2 m + 8 sm+ 2 st, 벌완두는 2 m + 2 sm + 2st, 나래완두는 3 m + 1 sm+ 2 st, 나비나물과 애기나비나물은 4 m + 6 sm + 2 st, 광릉갈퀴는 4 m + 8 sm로 각각 조사되었다.

• 방사선산업학회지
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• 제4권4호
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• pp.307-312
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• 2010

Plants have evolved physiological, biochemical and metabolic mechanisms to increase their survival under the adverse conditions. This present study has been performed to select salt-tolerant rice mutant lines through in vivo and in vitro mutagenesis with gamma-rays. For the selection of the salt-tolerant rice mutants, we conducted three times of selection procedure using 1,500 gamma ray mutant lines resulted from an embryo culture of the original rice cv. Dongan (wild-type, WT): first, selection in the a nutrient solution with 171 mM NaCl; second, selection under in vitro condition with 171 mM NaCl; and third, selection in a reclaimed saline land. Based on a growth comparison of the entries, out of the mutant lines, two putative 2 salt tolerant (ST) rice mutant lines, ST-87 and ST-301, were finally selected. The survival rate of the WT, ST-87 and ST-301 were 36.6%, 60% and 66.3% after 7 days in 171 mM NaCl treatment, respectively. The WT and two salt tolerant mutant lines were used to analyze their genetic variations. A total of 21 EcoRI and Msel primer combinations were used to analyze the genetic relationship of among the two salt-tolerant lines and the WT using the ABI3130 capillary electrophoresis system. In the AFLP analysis, a total of 1469 bands were produced by the 21 primer combinations, and 700 (47.6%) of them were identified as having polymorphism. The genetic similarity coefficients were ranged from 0.52 between the ST-87 and WT to 0.24 between the ST-301 and the WT. These rice mutant lines will be used as a control plot for physiological analysis and genetic research on salt tolerance.

• Journal of applied mathematics & informatics
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• 제26권1_2호
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• pp.307-316
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• 2008

Real life problems can be expressed as a congruence modulus n and split into a system of congruence equations in modulus factors of n. A system of congruence equations can be combined into a congruence equation under certain conditions. This paper uniquely presents and critically reviews the generalized Chinese Remainder Theorem (CRT) for combining systems of congruence equations into single congruence equations. Sequential and parallel implementation strategies of the generic CRT are outlined. A variety of unique applications of the CRT are discussed.

• 한국임상수의학회지
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• 제3권2호
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• pp.299-307
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• 1986

The each rumen of six goats was incised and sutured with chromic catgut, polyglycolic acid and nylon. Tissue reaction to each suture materials was observed and comared at the 7th, 14th and 21st post-operative days. The predominent inflammatory cells around suture materials are macrophages, fibroblast and neutrophils. A few lymphocytes was infiltrated around suture materials. Infiltration of neutrophils was rapidly diminished but infiltration of macrophages, fibroblasts and lymphocytes were persisted. The overall grade of tissue reaction varid by suture materials. At the 7th post-operative days, tissue reaction to chromic catgut was most prominant and that of polyglycolic acid and nylon was moderate. At the 14th and 21st postoperative days, tissue reaction to each suture materials was not greatly different.

• Anatomy and Cell Biology
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• 제56권3호
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• pp.304-307
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• 2023

The nervus conarii provides sympathetic nerve innervation to the pineal gland, which is thought to be the primary type of stimulus to this gland. This underreported nerve has been mostly studied in animals. One function of the nervus conarii may be to activate pinealocytes to produce melatonin. Others have also found substance P and calcitonin gene-related peptide from the nervus conarii ending in the pineal gland. The following paper reviews the extant medical literature on the nervus conarii including its anatomy and potential function.

• Anatomy and Cell Biology
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• 제56권3호
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• pp.350-359
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• 2023

The suboccipital triangle (ST) is a clinically relevant landmark in the posterior aspect of the neck and is used to locate and mobilize the horizontal segment of the third part of the vertebral artery before it enters the cranium. Unfortunately, this space is not always a viable option for vertebral artery exposition, and consequently a novel triangle, the inferior suboccipital triangle (IST) has been defined. This alternative triangle will allow surgeons to locate the artery more proximally, where its course is more predictable. The purpose of this study was to better define the anatomy of both triangles by measuring their borders and calculating their areas. Ethical clearance was obtained from the University of Pretoria (reference number: 222/2021) and both triangles were subsequently dissected out on both the left and right sides of 33 formalin-fixed human adult cadavers. The borders of each triangle were measured using a digital calliper and the areas were calculated using Herons Formula. The average area of the ST is 969.82±153.15 mm², while the average area of the IST is 307.48±41.31 mm². No statistically significant differences in the findings were observed between the sides of the body, ancestry, or sex of the cadavers. Measurement and analysis of these triangles provided important anatomical information and speak to their clinical relevance as surgical landmarks with which to locate the vertebral artery. Of particular importance here is the IST, which allows for mobilisation of this artery more proximally, should the ST be occluded.

• 한국IT서비스학회지
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• 제12권4호
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• pp.307-318
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• 2013

This study developed the prevention education program of smartphone overuse for adolescents in middle school and testified its outcome. Finally the 4-session prevention education program for smartphone overuse was developed. 1st session was composed of psychoeducation about smartphone overuse and its side effect by quiz question. 2nd session smartphone addiction and brain change, 3rd session motivational enhancement program for smartphone overuse, 4th session cognitive behavioral program for it. The outcome was testified by internet addiction scale, smartphone addiction scale, and school adaptation scale for 2 classes of middle school students(experimental and control class). 2 Main results were as follows; First, the smartphone addiction total score and cyberworld orientation of the students participating in it were statistically significantly reduced. The difficulty of daily living and abstinence symptom due to smartphone overuse of them tended to reduce. Second, internet addiction total and all subscores of them were not changed. Third, class adaptation of them was statistically significantly increased and school adaptation total score and teacher adaptation of them tended to increase. Its meaning, implication and limitation were discussed.