• 제목/요약/키워드: SSR markers

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토마토 유전자연관지도 상의 DarT 마커 분포 (Distribution of DArT Markers in a Genetic Linkage Map of Tomato)

  • Truong, Hai Thi Hong;Graham, Elaine;Esch, Elisabeth;Wang, Jaw-Fen;Hanson, Peter
    • 원예과학기술지
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    • 제28권4호
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    • pp.664-671
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    • 2010

  • 토마토풋마름병에 저항성인 $Solanum$ $lycopersicum$ H7996와 극도감수성인 $S.$ $pimpinellifolium$ WVa700 간의 교배를 통해 획득한 재조합순계계통 $F_9$ 세대의 188개체를 이용하여 유전자연관지도를 작성하였다. 유전자지도는 DarT 260종, AFLP 74종, RFLP 4종, SNP 1종 및 SSR 22종 등 총 361종의 마커로 구성되었다. 작성된 유전자지도는 총 13개의 연관군(LG)에 2042.7cM을 포함하였으며 마커간의 평균지도거리는 5.7cM이고 이중 DArT마커는 평균 7.9cM당 1개가 분포하였다. SSR 마커의 분포를 기초로 작성된 11개 연관군들은 토마토 염색체의5번과 12번을 제외한 10개 염색체에 해당하였다. DArT 마커는 다른 마커들처럼 토마토 유전체 상에 고르게 분포하였으며, 인접 마커와의 상호분석(${\leq}$ 0.5cM) 결과 클러스터링 빈도가 13.5%인 AFLP 마커보다 3배 정도 높은 38.8%의 빈도로 최고치를 나타내었다. 본 연구를 통해 토마토에서 최초로 DarT 마커를 이용한 유전자연관지도를 작성하였다.

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Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba)

  • Mahmoud, Amer F.;Abd El-Fatah, Bahaa E.S.
    • The Plant Pathology Journal
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    • 제36권1호
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    • pp.11-28
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    • 2020

  • Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

  • https://doi.org/10.5423/PPJ.OA.04.2019.0119 인용 PDF KSCI

Genetic diversity and phenotype variation analysis among rice mutant lines (Oryza sativa L.)

  • Truong, Thi Tu Anh;Do, Tan Khang;Phung, Thi Tuyen;Pham, Thi Thu Ha;Tran, Dang Xuan
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.22-22
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    • 2017

  • Genetic diversity is one of fundamental parameters for rice cultivar improvement. Rice mutants are also a new source for rice breeding innovation. In this study, ninety-three SSR markers were applied to evaluate the genetic variation among nineteen rice mutant lines. The results showed that a total of 169 alleles from 56 polymorphism markers was recorded with an average of 3.02 alleles per locus. The values of polymorphism information content (PIC) varied from 0.09 to 0.79. The maximum number of alleles was 7, whereas the minimum number of alleles was 2. The heterozygosity values ranged from 0.10 to 0.81. Four clusters were generated using the unweighted pair group method with arithmetic mean (UPGMA) clustering. Fourteen phenotype characteristics were also evaluated. The correlation coefficient values among these phenotye characteristics were obtained in this study. Genetic diversity information of rice mutant lines can support rice breeders in releasing new rice varieties with elite characterisitics.

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