Genetic diversity and phenotype variation analysis among rice mutant lines (Oryza sativa L.)

  • Truong, Thi Tu Anh (Graduate School for International Development and Cooperation, Hiroshima University) ;
  • Do, Tan Khang (Graduate School for International Development and Cooperation, Hiroshima University) ;
  • Phung, Thi Tuyen (Graduate School for International Development and Cooperation, Hiroshima University) ;
  • Pham, Thi Thu Ha (Graduate School for International Development and Cooperation, Hiroshima University) ;
  • Tran, Dang Xuan (Graduate School for International Development and Cooperation, Hiroshima University)
  • Published : 2017.06.04

Abstract

Genetic diversity is one of fundamental parameters for rice cultivar improvement. Rice mutants are also a new source for rice breeding innovation. In this study, ninety-three SSR markers were applied to evaluate the genetic variation among nineteen rice mutant lines. The results showed that a total of 169 alleles from 56 polymorphism markers was recorded with an average of 3.02 alleles per locus. The values of polymorphism information content (PIC) varied from 0.09 to 0.79. The maximum number of alleles was 7, whereas the minimum number of alleles was 2. The heterozygosity values ranged from 0.10 to 0.81. Four clusters were generated using the unweighted pair group method with arithmetic mean (UPGMA) clustering. Fourteen phenotype characteristics were also evaluated. The correlation coefficient values among these phenotye characteristics were obtained in this study. Genetic diversity information of rice mutant lines can support rice breeders in releasing new rice varieties with elite characterisitics.

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