• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.340-345
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• 1999

A novel simultaneous saccharification and fermentation (SSF) process from the microcrystalline cellulose to ethanol was developed by using $\delta$-integrated recombinant cellulolytic Saccharomyces cerevisiae L2612$L2612\deltaGC$, which can utilize cellulose as carbon and energy sources. The optimum amount of enzymes needed for the efficient conversion of cellulose to ethanol at $30^{\circ}C$ was determined with commercial cellulolytic enzymes. By fed-batch cultivation, the heterologous cellulolytic enzymes were accumulated up to 42.67% of the total cellulase and 29% of the $\beta$-glucosidase needed for the efficient SSF process. When this $\delta$-integrated recombinant yeast was applied to the successive SSF step for ethanol production, 20.35 g/l of ethanol was produced after 12 h from 50 g/l of microcrystalline cellulose. By using this novel SSF process, a considerable amount of commercial enzymes was reduced.

• 한국미생물·생명공학회지
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• 제44권2호
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• pp.145-149
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• 2016

해조류 중 홍조류인 K. alvarezii로부터 동시 당화 발효(SSF)를 위한 효소 당화 및 균 배양 온도를 검토하고, 기존의 동시 당화 발효(SSF) 를 개선하기 위해 2단계 동시 당화 발효(SSF)를 수행하였다. 효소 당화와 균 성장 온도를 고려하였을 때 동시 당화 발효(SSF)에 적용하는 배양 온도는 40°C를 선택하여 실험을 진행하였다. 비순치 효모(wild type)와 고농도 갈락토오스에 순치한 효모(adapted yeast to galactose)를 이용한 동시 당화 발효(SSF)를 실시한 결과 발효 156시간에 9.1 g/l의 에탄올 수율(Y_EtOH) 0.24와 10.2 g/l의 에탄올 수율(Y_EtOH) 0.27을 나타내었다. 이러한 기존의 동시 당화 발효(SSF)를 개선한 2단계 동시 당화 발효(SSF)는 에탄올 생산 수율이 0.27에서 0.35로 27.5% 증가하였으며, 에탄올 발효 시간도 156시간에서 96시간으로 61.5% 감소하였다. 이러한 연구결과는 해양 바이오매스인 해조류로부터 바이오연료 생산과정에 있어 기초적인 정보를 제공할 것이다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 추계학술대회 논문집
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• pp.240-243
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• 2001

Recently, many automobile parts produced by semi-solid forming(SSF) process has been applied for improved weight reduction, better environmental protection and energy savings. SSF process was well developed for high volume production of light weight aluminum components. In this paper, knuckle has been manufactured with A357 by SSF and thor investigated for microstructures and mechanical properties followed by various heat-treatment conditions. It was found that the examined microstructure was equiaxed at the whole cross-section area and as a result, the mechanical properties were satisfied by 100MPa YS, 260MPa UTS and $14\%$ elongation.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.40-47
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• 2005

Modeling and simulation for simultaneous saccharification and fermentation (SSF) process in bioconversion of paper mill sludge to lactic acid was carried out. The SSF process combined the enzymatic hydrolysis of paper mill sludge into glucose and the fermentation of glucose into lactic acid in one reactor. A mathematical modeling for cellulose hydrolysis was developed, based on the proposed mechanism of cellulase adsorption deactivation. Another model for simple lactic acid fermentation was also made. A whole mathematical model for SSF was developed by combining the above two models for cellulose hydrolysis and lactic acid fermentation. The characteristics of the SSF process were investigated using the mathematical model.

• 열처리공학회지
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• 제16권1호
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• pp.16-25
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• 2003

Recently, semi-solid forming (SSF) Process has been applied in many automobile parts for improved weight reduction, better environmental protection and energy savings. SSF process was well developed for high volume production of light weight aluminum components. In this paper, knuckle has been manufactured by SSF and then the microstructures and mechanical properties were investigated followed by various heat-treatment conditions. It was found that the examined microstructure was equiaxed at the whole cross-section area.

• 대한기계학회논문집A
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• 제21권9호
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• pp.1400-1413
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• 1997

Semi-solid forging(SSF) process of A356 aluminium alloy has been studied to assess the effect of process variables on the component integrity. Semi-solid material(SSM) was fabricated by mechanical and electro-magnetic stirring process. The fabricated SSM by using mechanical stirring process has been carried out on cooling rate of 0.022.deg. C/sec 0.0094.deg. C/sec and stirring speed n=600, 1000 rpm, respectively. The fabricated SSM by using electro-magnetic stirring process is supplied by Pechiney. The holding time and temperature in the semi-solid state before forging also affects the globular microstructure of alloy. Therefore, the influence of these two parameters is discussed in terms of the microstructure of alloy. The SSF process has been conducted with three different die temperatures($T_{die}$=250.deg. C, 300.deg. C, 350.deg. C) and two kinds of gate types(straight gate and orifice gate). This paper is to investigate the influence of gate shapes of die on filling phenomena in SSF process more deeply. The mechanical properties of forged components were also investigated for variation of process conditions such as die temperature, gate shape and SSM.

• 한국정밀공학회지
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• 제14권10호
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• pp.102-108
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• 1997

The technology of Semi-Solid Forging (SSF) has been actively developed to fabricate near-net- shape products using light and hardly formable materials. Generally, the SSF process is composed of slug heating, forming, compression holding and ejecting step. After forming step in SSF, the slug is compressed during a certain holding time in order to be completely filled in the die cavity and be accelerated in solidification rate. This paper presents the analysis of temperature, solid fraction and shrinkage at compression holding step for a cylindrical slug, then predicts the solidification time to obtain the final shaped part. Enthalpy-based finite element analysis is performed to solve the heat transfer problem considering phase change in solidification.

• Journal of Information Processing Systems
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• 제15권6호
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• pp.1503-1516
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• 2019

In this paper, to improve the accuracy of long sentence similarity calculation, we proposed a sentence similarity calculation method based on a system similarity function. The algorithm uses word2vector as the system elements to calculate the sentence similarity. The higher accuracy of our algorithm is derived from two characteristics: one is the negative effect of penalty item, and the other is that sentence similar function (SSF) based on word2vector similar elements doesn't satisfy the exchange rule. In later studies, we found the time complexity of our algorithm depends on the process of calculating similar elements, so we build an index of potentially similar elements when training the word vector process. Finally, the experimental results show that our algorithm has higher accuracy than the word mover's distance (WMD), and has the least query time of three calculation methods of SSF.

• 한국미생물·생명공학회지
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• 제9권2호
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• pp.65-69
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• 1981

농산폐자원을 이용하기 위한 방법으로써 Trichoderma sp. KI 7-2로 만든 koji와 내열성 효모를 사용하여 동시당화-발효와 당화액 발효를 비교하였다. 볏짚의 당화액에서는 15 mg/$m\ell$의 cellobiose가 존재하였으나 동일한 효소원을 사용한 동시당화-발효액에서는 존재하지 않았다. 효소당화법에서 는 cellulase enzyme system의 반응산물인 glucose가 cellobiose의 활성을 저해하므로 cellobiose 가 축적되었으나 동시당화-발효법에서 는 glucose가 ethanol로 발효되어 cellobiose의 축적이 없었다. Cutting mill 한 볏짚은 동시 당화-발효 과정에서는 ball mill 한 것과 같은 정도로 효과적으로 발효되었다. 이 결과로부터 cellylolytic enzyme system의 반응산물에 의한 저해 mechanism을 논의하였다. 또한 볏짚 당화시 약 10 mg/$m\ell$ 생산되는 xylose는 동시당화-발효에 아무런 영향을 미치지 않음을 확인하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.902-905
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• 2000

As the manufacturing processes of automotive engine piston, gravity die-casting, squeeze casting, hot forging and powder forging process are generally used for the various specifications. As the semi-solid forming(SSF) is compared with conventional casting such as gravity die-casting and squeeze casting for the characteristics of its process, the product without inner defects such as gas porosity and segregation can be obtained and its microstructure is globular grain. In SSF process, the materials are heated up to the temperature between the solvus and liquidus line at which the materials exists in the form of liquid-solid mixture. In this time, Discussion is given about reheating process of row material and results are presented regarding accurate temperature and process variables controlling for right solid fractions.