Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Novel SSF Process for Ethanol Production from Microcrystalline Cellulose Using the $\delta$-Integrated Recombinant Yeast, Saccharomyces cerevisiae L2612$\delta$GC

  • Cho, Kwang-Myung (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Institute for Molecular Biology and Genetics) ;
  • Yoo, Young-Je (Interdisciplinary Program for Biohemical Engineering and Biotechnology, Institute for Molecular Biology and Genetics, School of Chemical Engineering, Seoul National University)
  • Published : 1999.06.01
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Abstract

A novel simultaneous saccharification and fermentation (SSF) process from the microcrystalline cellulose to ethanol was developed by using $\delta$-integrated recombinant cellulolytic Saccharomyces cerevisiae L2612$L2612\deltaGC$, which can utilize cellulose as carbon and energy sources. The optimum amount of enzymes needed for the efficient conversion of cellulose to ethanol at $30^{\circ}C$ was determined with commercial cellulolytic enzymes. By fed-batch cultivation, the heterologous cellulolytic enzymes were accumulated up to 42.67% of the total cellulase and 29% of the $\beta$-glucosidase needed for the efficient SSF process. When this $\delta$-integrated recombinant yeast was applied to the successive SSF step for ethanol production, 20.35 g/l of ethanol was produced after 12 h from 50 g/l of microcrystalline cellulose. By using this novel SSF process, a considerable amount of commercial enzymes was reduced.

Keywords

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