• Title/Summary/Keyword: SSC

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Detection of Mendelian and Parent-of-origin Quantitative Trait Loci for Meat Quality in a Cross between Korean Native Pig and Landrace

  • Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, T.H.;Kim, K.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1644-1650
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) affecting meat quality in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 17 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between models were used to characterize the QTL for mode of gene expressions. A total of 10 (32) QTL were detected at the 5% genome (chromosome)-wise level for the analyzed traits. Of the 42 QTL detected, 13 QTL were classified as Mendelian, 10 as paternal, 14 as maternal, and 5 as partial expressed QTL, respectively. Among the QTL detected at 5% genome-wise level, four QTL had Mendelian mode of inheritance on SSCs 5, 10, 12, and 13 for cooking loss, drip loss, crude lipid and crude protein, respectively; two QTL maternal inheritance for pH at 24-h and shear force on SSC11; three QTL paternal inheritance for CIE b and Hunter b on SSC9 and for cooking loss on SSC15; and one QTL partial expression for crude ash on SSC13, respectively. Most of the Mendelian QTL (9 of 13) had a dominant mode of gene action, suggesting potential utilization of heterosis for genetic improvement of meat quality within the cross population via marker-assisted selection.

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

  • Park, Min Hee;Park, Ji Eun;Kim, Min Seong;Lee, Kwon Young;Hwang, Jae Yeon;Yun, Jung Im;Choi, Jung Hoon;Lee, Eunsong;Lee, Seung Tae
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.10
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    • pp.1398-1406
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    • 2016
  • In general, the seminiferous tubule basement membrane (STBM), comprising laminin, collagen IV, perlecan, and entactin, plays an important role in self-renewal and spermatogenesis of spermatogonial stem cells (SSCs) in the testis. However, among the diverse extracellular matrix (ECM) proteins constituting the STBM, the mechanism by which each regulates SSC fate has yet to be revealed. Accordingly, we investigated the effects of various ECM proteins on the maintenance of the undifferentiated state of SSCs in pigs. First, an extracellular signaling-free culture system was optimized, and alkaline phosphatase (AP) activity and transcriptional regulation of SSC-specific genes were analyzed in porcine SSCs (pSSCs) cultured for 1, 3, and 5 days on non-, laminin- and collagen IV-coated Petri dishes in the optimized culture system. The microenvironment consisting of glial cell-derived neurotrophic factor (GDNF)-supplemented mouse embryonic stem cell culture medium (mESCCM) (GDNF-mESCCM) demonstrated the highest efficiency in the maintenance of AP activity. Moreover, under the established extracellular signaling-free microenvironment, effective maintenance of AP activity and SSC-specific gene expression was detected in pSSCs experiencing laminin-derived signaling. From these results, we believe that laminin can serve as an extracellular niche factor required for the in vitro maintenance of undifferentiated pSSCs in the establishment of the pSSC culture system.

Estimation of Coastal Suspended Sediment Concentration using Satellite Data and Oceanic In-Situ Measurements

  • Lee, Min-Sun;Park, Kyung-Ae;Chung, Jong-Yul;Ahn, Yu-Hwan;Moon, Jeong-Eun
    • Korean Journal of Remote Sensing
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    • v.27 no.6
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    • pp.677-692
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    • 2011
  • Suspended sediment is an important oceanic variable for monitoring changes in coastal environment related to physical and biogeochemical processes. In order to estimate suspended sediment concentration (SSC) from satellite data, we derived SSC coefficients by fitting satellite remote sensing reflectances to in-situ suspended sediment measurements. To collect in-situ suspended sediment, we conducted ship cruises at 16 different locations three times for the periods of Sep.-November 2009 and Jul. 2010 at the passing time of Landsat $ETM_+$. Satellite data and in-situ data measured by spectroradiometers were converted to remote sensing reflectances ($R_{rs}$). Statistical approaches proved that the exponential formula using a single band of $R_{rs}$(565) was the most appropriate equation for the estimation of SSC in this study. Satellite suspended sediment using the newly-derived coefficients showed a good agreement with insitu suspended sediment with an Root Mean Square (RMS) error of 1-3 g/$m^3$. Satellite-observed SSCs tended to be overestimated at shallow depths due to bottom reflection presumably. This implies that the satellite-based SSCs should be carefully understood at the shallow coastal regions. Nevertheless, the satellite-derived SSCs based on the derived SSC coefficients, for the most cases, reasonably coincided with the pattern of in-situ suspended sediment measurements in the study region.

Review of Research for Herbal Medicine on Systemic Sclerosis (전신경화증의 한약치료에 대한 국내외 연구 동향)

  • Roh, Jong Seong;Kim, Sang Chan;Byun, Sung Hui;Yoon, Michung;Shin, Soon Shik;Sohn, Dong Hyun
    • Herbal Formula Science
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    • v.28 no.4
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    • pp.429-441
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    • 2020
  • Objectives : Systemic sclerosis(SSc) is an autoimmune disease characterized by fibrosis of the skin and internal organs and vasculopathy. The purpose of this study was to investigate the trend in the research on SSc using herbal medicine. Methods : We searched for papers which had both systemic sclerosis and herbal medicine from Pubmed, KCI and NDSL. After searching papers, we classified according to the study design and analyzed selected studies. Results : 18 studies were searched. The types and numbers of study were as follows: 11 were in vitro or in vivo studies using herbal medicine or active components, and 7 were clinical research including case reports. 1. Herbal medicines include the therapeutic effects of "tonifying qi(補氣)" or "active blood(活血)" improved systemic sclerosis in vivo and in vitro studies. 2. Active components isolated from Herbal Medicine such as Astragalus membranaceus(黃芪), Zhizi(梔子), Salvia miltiorrhiza(丹蔘) have anti-fibrotic effects. 3. Clinical trials showed that herbal medicine can improve the symptom of systemic sclerosis including skin fibrosis, Raynaud's phenomenon, pain and gastric dysmotility. Conclusions : This study showed that herbal medicine can be effective for treating SSc. However, further studies are needed to develop novel medicine for SSc.

A Melon Fruit Grading Machine Using a Miniature VIS/NIR Spectrometer: 1. Calibration Models for the Prediction of Soluble Solids Content and Firmness

  • Suh, Sang-Ryong;Lee, Kyeong-Hwan;Yu, Seung-Hwa;Shin, Hwa-Sun;Choi, Young-Soo;Yoo, Soo-Nam
    • Journal of Biosystems Engineering
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    • v.37 no.3
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    • pp.166-176
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    • 2012
  • Purpose: This study was conducted to investigate the potential of interactance mode of NIR spectroscopy technology for the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from local greenhouses in three different harvesting seasons (experiments 1, 2, and 3). The fruit attributes were measured at the 6 points on an equator of each sample where the spectral data were collected. The prediction models were developed using the original spectral data and the spectral data sets preprocessed by 20 methods. The performance of the models was compared. Results: In the prediction of SSC, the highest coefficient of determination ($R_{cv}{^2}$) values of the cross-validation was 0.755 (standard error of prediction, SEP=$0.89^{\circ}Brix$) with the preprocessing of normalization with range in experiment 1. The highest coefficient of determination in the robustness tests, $R_{rt}{^2}$=0.650 (SEP=$1.03^{\circ}Brix$), was found when the best model of experiment 3 was evaluated with the data set of experiment 2. The best $R_{cv}{^2}$ for the prediction of firmness was 0.715 (SEP=3.63 N) when no preprocessing was applied in experiment 1. The highest $R_{rt}{^2}$ was 0.404 (SEP=5.30 N) when the best model of experiment 3 was applied to the data set of experiment 1. Conclusions: From the test results, it can be concluded that the interactance mode of VIS/NIR spectroscopy technology has a great potential to measure SSC and firmness of thick-skinned muskmelons.

Short-term Variation of Sea Surface Temperature Caused by Typhoon Nabi in the Eastern Sea of Korean Peninsula Derived from Satellite Data (위성영상에서 관측한 태풍 Nabi 통과시의 한반도 동부해역 수온의 단기변동)

  • Kim, Sang-Woo;Yamada, Keiko;Jang, Lee-Hyun;Hong, Chul-Hoon;Go, Woo-Jin;Suh, Young-Sang;Lee, Chu;Lee, Gyu-Hyong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.2
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    • pp.102-107
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    • 2007
  • A remarkable sea surface cooling (SSC) event was observed in the eastern sea of Korean peninsula based on new generation sea surface temperature (NGSST) satellite images in September 2005, when typhoon Nabi passed over the East Sea. The degree of SSC ranged from $1^{\circ}C\;to\;4^{\circ}C$, and its maximum was observed in the southeastern sea area. Daily variations in sea surface temperature at a longitudinal line $(35^{\circ}-41^{\circ}N,\;132^{\circ}E)$, derived from satellite data for September 1-13, 2005, showed that the SSC lasted about 3 days after the typhoon passed in the south of $39^{\circ}N$, whereas it was unclear in the north of$39^{\circ}N$. Water temperature measured by a mooring buoy suggested that the SSC was caused mainly by a vertical mixing of the water column driven by the typhoon, rather than by coastal upwelling.

Topical anesthesia for stainless steel crown tooth preparation in primary molars: a pilot study

  • Padminee, Krishnan;Hemalatha, R;Shankar, P;Senthil, D;Trophimus, Gnanabagyan J
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.20 no.4
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    • pp.241-250
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    • 2020
  • Background: Placement of full-coverage restorations such as stainless steel crowns (SSCs) for pulpectomy treated primary molars is essential for successful outcomes. The tooth preparation process for SSCs can cause discomfort to gingival tissues since the crown should be seated 1 mm subgingivally. The purpose of this prospective trial was to compare the effectiveness of subgingival and transmucosal application of topical anesthetics on dental pain during SSC tooth preparation among 6- to 8-year-old children. Methods: A consecutive sample of 27 children, aged 6-8 years, who required an SSC after pulp therapy in primary molars were randomly divided into three groups. Group A received infiltration anesthesia before tooth preparation for SSC placement, whereas in Group B and C, only topical anesthesia was applied subgingivally and transmucosally. Wong-Bakers Faces pain rating scale (WBFPS) scores were recorded after tooth preparation. Faces, Legs, Activity, Cry and Consolability (FLACC) scores were evaluated by two blinded and calibrated investigators through video recordings of the patient during tooth preparation. Data were tabulated, and inter-group comparisons were performed using the Kruskal-Wallis and analysis of variance tests. Results: Out of the 27 participants, 48% were boys and 52% were girls, with an overall mean age of 6.83 years. Group A showed the least pain scores according to both the scales, followed by Group B and Group C. The pain intensity was statistically significant on both the pain scales with P = 0.003 for FLACC and P < 0.001 for WBFPS. Conclusion: Subgingival application of topical anesthesia reduced pain to a certain extent but not as effectively as infiltration anesthesia during SSC tooth preparation in primary molars. Transmucosal application of topical anesthesia did not reduce discomfort when compared to the other two interventions.

A Design of Initial Cell Searcher for 3GPP LTE Downlink System (3GPP LTE 하향링크 시스템을 위한 초기 셀 탐색기 설계)

  • Shin, Kyung-Chan;Im, Se-Bin;Ok, Kwang-Man;Choi, Hyung-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.7A
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    • pp.733-742
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    • 2008
  • In 3GPP LTE downlink system, initial cell search is essential for mobile station to connect to base station. In order to obtain information of the base station, the mobile station detects frame timing, frequency offset, and cell identification using primary synchronization channel(PSC) and secondary synchronization channel(SSC), which are defined in downlink OFDMA specification. In this paper, we analyze various detection algorithms in practical environment of inter-cell-interference, frequency offset, and multi-path fading channel and propose the optimal algorithm. Simulation results show that partial correlation method (for PSC acquisition) and interference cancellation method (for SSC detection) are the most superior algorithms among the applicable algorithms. Employ these two algorithms for receiver design, initial cell search is performed with 99% probability within 70ms in the channel environment considered.

Study on the Optimization of Spent Sulfidic Caustic Applied for BNR Process (Spent Sulfidic Casutic의 BNR 공정 적용을 위한 최적화 연구)

  • Lee, Jae-Ho;Ju, Dong-Jin;Park, Jeung-Jin;Shin, Choon-Hwan
    • Journal of Environmental Science International
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    • v.20 no.12
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    • pp.1617-1624
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    • 2011
  • Caustic (NaOH) solution is used to remove $H_2S$ from hydrocarbon streams in petroleum refining industry, gradually being, so called, spent sulfidic caustic (SSC) which has high levels of $H_2S$ and alkalinity. Thus, SSC can be used as an electron donor and a buffering agent for autotrophic denitrification. As SSC, however, contains some non-biodegradable organics, air stripping was conducted to remove the non-biodegradable organics. As a result, over 93 % of the non-biodegradable organics was removed within 30 min of aeration. Then, $Na_2S_2O_3{\cdot}5H_2O$, methanol and organic matters, which are produced from a biodiesel production plant, were added to reform the air-stripped SSC and their products being referred to new sulfidic caustics (NSCs) I, II and III, respectively. Thereafter, to investigate the effect of these products on the removal of COD and TN, these products were injected to a biological nitrogen removal (BNR) process, resulting in additional 44 % TN removal without noticeable increase in the effluent COD level. Therefore, it can be said that the BNR process is a promising option to treat NSC as demonstrated in this study whose results can be useful for developing resource recovery technologies.

Detection of Quantitative Trait Loci Affecting Fat Deposition Traits in Pigs

  • Choi, B.H.;Lee, K.T.;Lee, H.J.;Jang, G.W.;Lee, H.Y.;Cho, B.W.;Han, J.Y.;Kim, T.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.11
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    • pp.1507-1510
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    • 2012
  • Quantitative trait loci (QTL) associated with fat deposition traits in pigs are important gene positions in a chromosome that influence meat quality of pork. For QTL study, a three generation resource population was constructed from a cross between Korean native boars and Landrace sows. A total of 240 F2 animals from intercross of F1 were produced. 80 microsatellite markers covering chromosomes 1 to 10 were selected to genotype the resource population. Intervals between adjacent markers were approximately 19 cM. Linkage analysis was performed using CRIMAP software version 2.4 with a FIXED option to obtain the map distances. For QTL analysis, the public web-based software, QTL express (http://www.qtl.cap.ed.ac.uk) was used. Two significant and two suggestive QTL were identified on SSC 6, 7, and 8 as affecting body fat and IMF traits. For QTL affecting IMF, the most significant association was detected between marker sw71 and sw1881 on SSC 6, and a suggestive QTL was identified between sw268 and sw205 on SSC8. These QTL accounted for 26.58% and 12.31% of the phenotypic variance, respectively. A significant QTL affecting IMF was detected at position 105 cM between markers sw71 and sw1881 on SSC 6.