• The Journal of Pediatrics of Korean Medicine
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• v.5 no.1
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• pp.15-27
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• 1991

Experimental studies were done to research the clinical effects of Yongdamsagantang and Yongdamsagantang-gamibang (Yongdamsagantang with Lonicerae Flos and Forsythiae Fructusadded) on the Anti-allergic effect in rats and mice. The results obtained as follows; 1. In the effects of Yongdamsagantang and Yongdamsagantang-gamibang on vascular permeability responses to intradermal histamine in rats, Yongdamsagantang group revealed none significant affect, but Yongdamsagantang-gamibang group revealed significant effect. 2. In the effects of Yongdamsagantang and Yongdamsagantang-gamibang on vascular permeability responses to intradermal serotonin in rats, Yongdamsagantang and Yongdamsagantang-gamibang group revealed significant effect. 3. In the 48hrs homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody against egg albumin, Yongdamsagantang group revealed none significant effect, but Yongdamsagantang-gamibang group revealed significant effect. 4. In the delayed type hypersensitivity responses to Picryl Chloride in mice, Yongdamsagantang and Yongdamsagantang-gamibang group revealed none significant effect, in the delayed type hypersensitivity responses to SRBC in mice, Yongdamsagantang group revealed none significant effect, but Yongdamsagantang-gamibang group revealed significant effect. According to above-stated results, Yongdamsagantang is none significant Anti-allergic effect. But Yongdamsagantang-gamibang is concluded to be effective as immediated type hypersensitivity and recommended to be used for the treatment of allergic diseases. (Asthma, Allergic urticaria, Allergic rhinitis, etc.)

• The Journal of Pediatrics of Korean Medicine
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• v.8 no.1
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• pp.27-37
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• 1994

This Experimental study was done to investigate the effect of the Taklisodok-um on the Anti-allergic response. The results were obtained as follows: 1. On vascular permeability response to the intradermal injected Histamine, the Taklisodok-um treated group revealed more significant decrease than control group. 2. On vascular permeability response to the intradermal injected Serotonin, the Taklisodok-um treated group revealed more significant decrease than control group. 3. In the 48hrs homologous passive cutaneous anaphylaxis provoked by the IgE-like antibody against white egg albumin, there was no significant difference between the Taklisodok-um treated group and control group. 4. The Taklisodok-um treated group revealed more significant inhibitory effect than control group in the delayed type hypersensitivity response to Picryl chloride. 5. The Taklisodok-um treated group revealed more significant inhibitory effect than control group in the delayed type hypersensitivity response to SRBC. According to the above-stated results, it is considered that the Taklisodok-um could be applied widely to the type I and IV allergic diseases.

• The Journal of Pediatrics of Korean Medicine
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• v.8 no.1
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• pp.13-26
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• 1994

Experimental studies were done to research the clinical effects of Sipyungtang and Sipyungtang-gamibang (sipyungtang with Anemarrhenae Rhizoma added) on the Anti-allergic effect. The results obtained as follows; 1. In the effects of Sipyungtang and Sipyungtang-gamibang on vascular permeability responses to intradermal Histamine, Sipyungtang group revealed none significant effect, but Sipyungtang-gamibang group revealed significant effect. 2. In the effects of sipyungtang and Sipyungtang-gamibang on vascular permeability responses to intradermal serotonin, Sipyungtang group revealed none significant effect, but Sipyungtang-gamibang group revealed significant effect. 3. In the 48hrs homologous passive cutaneous anaphylaxis provoked by the IgE-like antibody against egg white albumin, Sipyungtang and Sipyungtang-gamibang groups revealed none significant effect. 4. In the delayed type hypersensitivity responses to Picryl Chloride, Sipyungtang and Sipyungtang-gamibang groups revealed none significant effect. 5. In the delayed type hypersensitivity responses to SRBC, Sipyung-tang and Sipyungtang-gamibang groups revealed significant effect.

• YAKHAK HOEJI
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• v.33 no.1
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• pp.30-38
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• 1989

The cellular and nonspecific immune response of EPA were investigated in mice. ICR male mice were divided into 8 groups and received intraperiteneal injection of EPA (5 mg, 10 mg, 20 mg/kg) for 4 weeks. Cyclophosphamide (5 mg/kg) was administered i.p. 2days prior to secondary immunization. Immune responses were evaluated by hypersensitivity to SRBC(DTH), rosette forming cell(RFC), natural killer(NK) cell activity and macrophage activity. The obtanined results were as follows: As compared to normal group, 1) DTH was increased by EPA 5 mg, 10 mg administration groups. 2) RFC was significantly increased by EPA 20 mg administration group. 3) NK-Cell activity was significantly increased by EPA 10 mg administration group. 4) Macrophase activity was enhanced by EPA 5 mg administration group.

• Biomolecules & Therapeutics
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• v.9 no.3
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• pp.194-200
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• 2001

Phellinus linteus (PL)-hot water extract (PL-W) or- methanol extract (PL-M) was orally administered alone (single dose of 400, 800, 1600 mg/kg; 800 mg/kg/day for 5 days) or with cyclophosphamide (CY, 20 mg/kg, i.p.) to female ICR mice. Within PL alone-treated group, WBC and plaque forming cells (PFC) to SRBC were slightly and significantly enhanced when compared with control group. The relative thymus and spleen weights, WBC and PFC numbers were significantly decreased by the treatment of CY, whereas those values were markedly increased by the concomitant treatment of CY and PL when compared with CY administration alone. To assess the effects of PL and/or CY on the mitogen response of splenocytes io LPS, mouse splenocytes were stimulated with or without LPS in the presence of various concentration of PL and/or CY in vitro and splenocytes proliferation (SP) was measured by MTT assay. PL alone increased both SP and LPS- stimulated SP. Moreover, SP and LPS-induced SP suppressed by the treatment of CY alone were significantly restored by PL-treatment. These activities were higher by PL-M than by PL-W, These results indicated that PL was able to increase humoral immunity and to inhibit immunotoxicity induced by CY.

• The Journal of Pediatrics of Korean Medicine
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• v.6 no.1
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• pp.1-14
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• 1992

Experimental studies were done to research the clinical effects of Juguieum and Juguieum-gamibang(Juguieum with Lonicerae Flos and Forsythiae Fructus added) on the Antiallergic effect. The results obtained as follows; 1. In the effects of Jugieum and Jugieum-gamibang on vascular permeability responses to intradermal histamine, Juguieum group revealed none significant effect, but Jugieum-gamibang group revealed significant effect. 2. In the effects of Juguieum and Juguieum-gamibang on vascular permeability responses to intradermal serotonin, Juguieum and Juguieum-gamibang groups revealed significant effect. 3. In the 48hrs homologous passive cutaneous amaphylaxis provoked by the IgE-like antibody against egg albumin, Juguieum and Juguieum-gamibang groups revealed significant effect. 4. In the delayed type hypersensitivity responses to Picryl Chloride, Juguieum and Juguieum-gamibang groups revealed significant effect. 5. In the delayed type hypersensitivity responses to SRBC, Juguieum group revealed significant effect, but Jugieum-gamibang group revealed none significant effect. According to above-stated results, Juguieum and Juguieum-gamibang are concluded to be effective as anti-allergic regimen and recommended to be used for the treatment of type I and type IV allergic disease.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.6
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• pp.1604-1609
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• 2005

The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.

• The Journal of Internal Korean Medicine
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• v.15 no.2
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• pp.260-273
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• 1994

This study attempted to investigate the effects of Sojagangkitang and Gamisojagangkitang on the variation of lung thiobarbituric acid(TBA) value, tracheal glycoprotein, serum sodium ion$(Na^+)$ contents, serum potassium ion$(K^+)$ contents ; immediatly type allergy reaction, delayed type allergy reaction in rats and mice. The results were as follows: 1. Sojagangkitang and Gami-sojagangkitang revealed significant effect on immediatly type hypersensitivity responds to histamine. 2. Sojagangkitang and Gami-sojagangkitang revealed significant effect on delayed type hypersensitivity responds to picryl chloride. 3. Sojagangkitang and Gami-sojagangkitang revealed significant effect on delayed type hypersensitivity responds to SRBC, effect of Gami-sojagangkitang was outstanding. 4. Lung thiobarbituric acid(TBA) value was decreased with statistical significance. 5. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on Tracheal glycoprotein contents, effect of Gami-sojagangkitang was outstanding. 6. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on phenol red excretion of respiratory tract. 7. Viscosity of mucine solution was decreased in proportion to increasing dosage of the Sample. 8. Serum $Na^+$ contents was not recognized significance. 9. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on Serum $K^+$ contents, effect of Gami-sojagangkitang were outstanding. According to the above results, it seems that Sojagangkitang and Gami-sojagangkitang can be applied for asthma, chronic obstructive pulmonary diseases, allergic respiratory diseases.

• Archives of Pharmacal Research
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• v.13 no.4
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• pp.330-337
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• 1990

The crude polysaccharide from Panax ginseng prepared by hot water extration and precipiation with ethanol was further fractionated into neutral and acidic fractions by DEAE- cellulose ion exchange chromatography. The chemical compositions were 85.0% carbohydrorate and 15.0% protein for the neutral fraction, and 28.4% carbohydrate, 10.0% protein and 29.0% uronic acid for the acidic fraction. The acidic fraction was more effective in increasing of the ratio of spleen to body weight, the number of antibody secreting cells to SRBC and phagocytic activity of reticuloendothelial system, as well as antitumor activity against the solid form of sarcoma 180 in ICR mice than the neutral fraction. All polysaccharide fractions were mitogenic to cultured spleen cells of C57BL/6 mice. However, FA was different from FN in the co-mitogenicities with lectin mitogens. Both crude and acidic fractions potentiated remarkably the mitogenic activity of PHA-P or LPS in dose-dependent manner but neutral fraction enhanced only that of LPS. Three polysaccharide fractions had no effect on that of Con A. These results suggest that the acidic fraction may stimulate B and Td cells as well as macrophages while the neutral fraction may simulate only B cells and macropages.

• Journal of Acupuncture Research
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• v.18 no.2
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• pp.136-149
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• 2001

Objetives : This study was done to evaluate the antiallegic effect of medicinal acupuncture solution of Acanthopanax senticosus(Rupr. et Maxim;abbreviated as ASMA), studies were done experimentally. Methods : For this aim MTT assay, anaphylaxis reaction, DTH, histamine release, IgE production and vascular permability was evaluated. Results : ASMA didn't show an effective cytotoxicity on the mouse lung fibroblast. It insignifcantly suppressed histamine release from mast cells induced by compound 48/80, while it significantly reduced IgE production and SRBC-challenged DTH(Delayed type hypersensitivity). ASMA effectively suppressed anaphylactic reaction induced by compound 48/80. However, it didn't inhibit vascular permeability and contact dermatitis significantly. Conclusion:Medicinal acupucture solution had antialletgic effects. Acanthopanax senticosus medical acupuncture depress the allergy reaction. Especially in anaphylactic type, the effect was good.