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Characterization of a PyrR-deficient Mutant of Bacillus subtilis by a Proteomic Approach (프로테옴 분석에 의한 Bacillus subtilis PyrR 돌연변이체의 특성)

  • Seul, Keyung-Jo;Cho, Hyun-Soo;Ghim, Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.39 no.1
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    • pp.9-19
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    • 2011
  • The Bacillus subtilis pyrimidine biosynthetic (pyr) operon encodes all of the enzymes for the de novo biosynthesis of Uridine monophosphate (UMP) and additional cistrones encoding a uracil permease and the regulatory protein PyrR. The PyrR is a bifunctional protein with pyr mRNA-binding regulatory funtion and uracil phosphoribosyltransferase activity. To study the global regulation by the pyrR deletion, the proteome comparison between Bacillus subtilis DB104 and Bacillus subtilis DB104 ${\Delta}$pyrR in the minimal medium without pyrimidines was employed. Proteome analysis of the cytosolic proteins from both strains by 2D-gel electrophoresis showed the variations in levels of protein expression. On the silver stained 2D-gel with an isoelectric point (pI) between 4 and 10, about 1,300 spots were detected and 172 spots showed quantitative variations in which 42 high quantitatively variant proteins were identified. The results showed that production of the pyrimidine biosynthetic enzymes (PyrAA, PyrAB, PyrB, PyrC, PyrD, and PyrF) were significantly increased in B. subtilis DB104 ${\Delta}$pyrR. Besides, proteins associated carbohydrate metabolism, elongation protein synthesis, metabolism of cofactors and vitamins, motility, tRNA synthetase, catalase, ATP-binding protein, and cell division protein FtsZ were overproduced in the PyrR-deficient mutant. Based on analytic results, the PyrR might be involved a number of other metabolisms or various phenomena in the bacterial cell besides the pyrimidine biosynthesis.

Sources of Carbonaceous Materials in the Airborne Particulate Matter of Dhaka

  • Begum, Bilkis A.;Hossain, Anwar;Saroar, Golam;Biswas, Swapan K.;Nasiruddin, Md.;Nahar, Nurun;Chowdury, Zohir;Hopke, Philip K.
    • Asian Journal of Atmospheric Environment
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    • v.5 no.4
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    • pp.237-246
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    • 2011
  • To explore the sources of carbonaceous material in the airborne particulate matter (PM), comprehensive PM sampling was performed (3 to 14 January 2010) at a traffic hot spot site (HS), Farm Gate, Dhaka using several samplers: AirMetrics MiniVol (for $PM_{10}$ and $PM_{2.5}$) and MOUDI (for size fractionated submicron PM). Long-term PM data (April 2000 to March 2006 and April 2000 to March 2010 in two size fractions ($PM_{2.2}$ and $PM_{2.2-10}$) obtained from two air quality-monitoring stations, one at Farm Gate (HS) and another at a semi-residential (SR) area (Atomic Energy Centre, Dhaka Campus, (AECD)), respectively were also analyzed. The long-term PM trend shows that fine particulate matter concentrations have decreased over time as a result of government policy interventions even with increasing vehicles on the road. The ratio of $PM_{2.5}/PM_{10}$ showed that the average $PM_{2.5}$ mass was about 78% of the $PM_{10}$ mass. It was also found that about 63% of $PM_{2.5}$ mass is $PM_1$. The total contribution of BC to $PM_{2.5}$ is about 16% and showed a decreasing trend over the years. It was observed that $PM_1$ fractions contained the major amount of carbonaceous materials, which mainly originated from high temperature combustion process in the $PM_{2.5}$. From the IMPROVE TOR protocol carbon fraction analysis, it was observed that emissions from gasoline vehicles contributed to $PM_1$ given the high abundance of EC1 and OC2 and the contribution of diesel to $PM_1$ is minimal as indicated by the low abundance of OC1 and EC2. Source apportionment results also show that vehicular exhaust is the largest contributors to PM in Dhaka. There is also transported $PM_{2.2}$from regional sources. With the increasing economic activities and recent GDP growth, the number of vehicles and brick kilns has significantly increased in and around Dhaka. Further action will be required to further reduce PM-related air pollution in Dhaka.

Plasma-Assisted Molecular Beam Epitaxy of InXGa1-XN Films on C-plane Sapphire Substrates (플라즈마분자선에피탁시법을 이용한 C-면 사파이어 기판 위질화인듐갈륨박막의 에피탁시 성장)

  • Shin, Eun-Jung;Lim, Dong-Seok;Lim, Se-Hwan;Han, Seok-Kyu;Lee, Hyo-Sung;Hong, Soon-Ku;Joeng, Myoung-Ho;Lee, Jeong-Yong;Yao, Takafumi
    • Korean Journal of Materials Research
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    • v.22 no.4
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    • pp.185-189
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    • 2012
  • We report plasma-assisted molecular beam epitaxy of $In_XGa_{1-X}N$ films on c-plane sapphire substrates. Prior to the growth of $In_XGa_{1-X}N$ films, GaN film was grown on the nitride c-plane sapphire substrate by two-dimensional (2D) growth mode. For the growth of GaN, Ga flux of $3.7{\times}10^{-8}$ torr as a beam equivalent pressure (BEP) and a plasma power of 150 W with a nitrogen flow rate of 0.76 sccm were fixed. The growth of 2D GaN growth was confirmed by $in-situ$ reflection high-energy electron diffraction (RHEED) by observing a streaky RHEED pattern with a strong specular spot. InN films showed lower growth rates even with the same growth conditions (same growth temperature, same plasma condition, and same BEP value of III element) than those of GaN films. It was observed that the growth rate of GaN is 1.7 times higher than that of InN, which is probably caused by the higher vapor pressure of In. For the growth of $In_xGa_{1-x}N$ films with different In compositions, total III-element flux (Ga plus In BEPs) was set to $3.7{\times}10^{-8}$ torr, which was the BEP value for the 2D growth of GaN. The In compositions of the $In_xGa_{1-x}N$ films were determined to be 28, 41, 45, and 53% based on the peak position of (0002) reflection in x-ray ${\theta}-2{\theta}$ measurements. The growth of $In_xGa_{1-x}N$ films did not show a streaky RHEED pattern but showed spotty patterns with weak streaky lines. This means that the net sticking coefficients of In and Ga, considered based on the growth rates of GaN and InN, are not the only factor governing the growth mode; another factor such as migration velocity should be considered. The sample with an In composition of 41% showed the lowest full width at half maximum value of 0.20 degree from the x-ray (0002) omega rocking curve measurements and the lowest root mean square roughness value of 0.71 nm.

Spatial Analysis of the Difference between Real Crime and Fear of Crime (도시내 범죄발생과 범죄 두려움 위치의 공간적 차이 분석)

  • Heo, Sun-Young;Moon, Tae-Heon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.14 no.4
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    • pp.194-207
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    • 2011
  • This study tries to find the possibility to prevent crime by improving urban spatial environment through the analysis of spatial environment property that mutually coincides or differs by comparing the place where crime actually occurs and the place where citizen is afraid of crime. The method of study is as follows. First, the ontents scope and method of study was established by theoretic investigation of case study related to crime. Second, as crime cannot be prevented by police power only, CPSCP(Citizen Participation System for Crime Prevention) was developed so that all citizen can cooperatively participate in the crime prevention anytime and anywhere. Third, the data on the place where people feel fear in the region was collected by directly indicating the place where citizen is afraid of crime in the space by utilizing CPSCP. Fourth, the place where crime actually occurs and the place where citizen is afraid of crime are redundantly analyzed for comparative analysis of 2 places. The result shows that environmental design improving physical environment of urban space is necessary to prevent crime and to eliminate the fear of crime. The CPSCP developed by this study which will be advanced to U-crime prevention system will contribute to making citizen's own neighborhood a smart safety city autonomously.

Effect of a Fluoride - Containing Orthodontic Primer for Preventing Enamel Demineralization around Bracket (불소를 함유한 교정용 접착제의 브라켓 주변 법랑질 탈회 예방 효과)

  • Jang, Hayoung;Kim, Jongbin;Kim, Jongsoo;Oh, Sohee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.44 no.4
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    • pp.412-418
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    • 2017
  • During orthodontic treatment, formation of white spot lesions (WSLs) around brackets has long been recognized as a potential risk. This study performed to investigate effect of preventing enamel demineralization and remineralization by application of fluoride-containing orthodontic primer. Fifty extracted bovine incisors teeth were randomly allocated to 3 groups: (I) Non-preparation specimens, (II) Application of Light Bond$^{TM}$ as fluoride containing orthodontic primer, (III) Application of Transbond$^{TM}$ XT Primer as traditional orthodontic primer without fluoride. Each group is demineralized under artificial carious solution. The demineralization pattern was evaluated using a Q-ray view, Vickers hardness test and polarized light microscope. The remained primer was calculated as 35 - 50%. The highest surface microhardness was shown on Light Bond$^{TM}$ surface. There were statistically significant differences in Vickers microhardness number between adjacent areas of Light Bond$^{TM}$ and non-prepared area. There was almost no demineralization of the enamel surface under the Light Bond$^{TM}$. At the adjacent site of Light Bond$^{TM}$, the shallow caries pattern and remineralization appearance were also observed. These results suggest that the use of fluoride-containing primers may be useful for bracket attachment to reduce enamel demineralization during orthodontic treatment.

A STUDY ON STRESS DISTRIBUTION OF ENDODONTICALLY TREATED TOOTH ACCORDING TO THE VARIOUS POST LENGTH USINGTHREE-DIMENSIONAL FINITE ELEMENT METHOD (포스트 길이가 치근내 응력분산에 미치는 영향에 관한 삼차원 유한요소법적 연구)

  • Choi, Soo-Yong;Lee, Sun-Hyung;Yang, Jae-Ho;Chung, Hun-Young
    • The Journal of Korean Academy of Prosthodontics
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    • v.33 no.2
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    • pp.177-197
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    • 1995
  • The endodontically treated tooth is generally restored with post & core, owing to the brittleness and the loss of large amount of tooth structure. Although there have been lots of studies about the endodontically treated teeth, the three-dimensional quantitative studies about the strees distribution of them are in rare cases. In this study, it was assumed that the coronal portion of the upper incisou had severely damaged. After the root canal therapy it was post cored, and restored with PFM crown, for this experiment nine types of model were constructed : 1); long, 2); medium, 3); short gold post for the roots supported with a narmal alveolar bone, 4); long, 5); medium, 6); short gold post for the roots supported with an alveolar bone resorbed to its 1/3 of root length, 7); long, 8); medium, 9); short base metal post for the roots supported with an alveolar bone resorbed to its 1/3 of root length. Force was applied from two directions. One was functional maximum bite force(300N) applied to the spot just lingual to the incisal edge with the angle of 45 degrees to the long axis of the tooth, and the other one was horizontal force(300N) applied to the labial surface. The results analyzed with three-dimensional finite element method were as follows : 1. Stress was concentrated on the middle portion of the labial side dentin of the root and the lingual portion of the apical dentin of the root. Stress in the post showed maximum value at 2 mm above the post apex. 2. In case of the long post and base metal post, strees was concentrated on the apex of the root and the post. 3. In case of the longer post, the displacement on the post-cement interface was lessened. The gold post was more displaceable than the base metal post. 4. In case of the alveolar bone resorption, stress concentrated on the root and the post and displacement on the post-cement interface were increased.

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A New Cultivar Hibiscus syriacus 'Red Bohanjae' with Small Violet-Pink Flowers for a Pot Plant (분화용 무궁화 자색 단심 'Red Bohanjae' 육성)

  • Ha, Yoo Mi;Kim, Dong Yeob;Shim, Kyung Ku
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.292-297
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    • 2015
  • A new cultivar of Hibiscus 'Red Bohanjae' (Hibiscus syriacus 'Red Bohanjae') was developed after crossing between Hibiscus syriacus 'Andong' (♀) with white flowers and Hibiscus syriacus 'Samchully' (♂) with pink flowers to improve flower quality and growth habit. The Hibiscus breeding program was initiated in 2002, and H. syriacus 'Red Bohanjae' was preliminarily selected as '02-RV-17' in 2003 due to its stable flower quality with rich pink flowers and small tree type. The selected line was further evaluated for growth characteristics, leaf shape, leaf size, flowering characteristics, and tested for distinctness, homogeneity, and stability during 2004-2007. The characteristics preserved by grafting. 'Red Bohanjae' showed a small growth habit with slightly upright branches. The tree had potential use as a potted plant or bonsai because of its dwarf form. The new cultivar 'Red Bohanjae' had violet-pink color flowers (RHS N78C) with red eye spots, small size (9.2 cm) and spatulate (I-type) petals. Leaves were 4.2 cm long and 2.3 cm wide. After the evaluation of plant characteristics for 3 years (2005-2007), it was registered as cultivar 'Red Bohanjae' (4118, plant variety protection rights number) in 2012. 'Red Bohanjae' having violet-pink flowers with red eye and dwarf tree form seems to be suitable for pot or bonsai cultivation in both indoor and outdoor conditions.

The effect of self-regulatory focus on loss aversion when gain and loss between possession and valence compete (소유와 유인가의 득과 실이 경쟁할 때 자기조절초점이 손실회피에 미치는 효과)

  • Lee, Byung-Kwan;Lee, Guk-Hee
    • Science of Emotion and Sensibility
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    • v.17 no.4
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    • pp.101-108
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    • 2014
  • The study of loss aversion has progressed. Specifically, Brenner et al. (2007) distinguished between possession loss aversion (PLA) and valence loss aversion (VLA) and explained PLA is the loss of cash or spot goods (money, apartment) and that VLA is the loss of tentative value (opportunity of oversea study, coupon). Also, they explored how the tendency of loss aversion was different between when possession loss was salience and when valence loss was outstanding. But, in our everyday life, it is more common that people face some economic situation where the gain and loss between possession and valence are competing. And, in this situation, they usually choose a better option based on their ordinary mind-set or self-focus rather than on which option has more benefit. Therefore, present research is that whether the tendency of loss aversion is different based on one's ordinary self-focus. Concretely, after priming promotion focus vs. prevention focus, participants should decide whether present occupation changes or not when gain and loss between possession and valence compete (income increase but oversea study chance decrease or income decrease but oversea study chance increase). As a result, there are more rate of changing job in promotion focusing condition than prevention focusing condition based on positive change irrespective of possession or valence. This result can have implication for marketing, consumer and advertisement psychology.

Investigation of Reportable Communicable Diseases and Parasites in Aquatic Organisms Living in the Estuary of the Han River (한강 하구에 서식하는 수산생물의 법정전염병 및 기생충 감염 조사)

  • Kim, Jin Hui;Song, Jun Young;Lee, Jung-Ho;Hur, Jun Wook;Kwon, Se Ryun;Kwon, Joon Yeong
    • Korean Journal of Ecology and Environment
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    • v.52 no.4
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    • pp.306-315
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    • 2019
  • The estuary of the Han River constantly suffers from pollutants and pathogenic microorganisms which could cause serious damage to aquatic organisms living there. Despite of this potential risk, it is hard to find any reliable scientific reports on the status of reportable disease infection to the organisms living in this area. In this study, cyprinid fish and crustaceans in Jeonryu-ri, a region of the Han River estuary, were investigated for the infection by representative reportable communicable diseases(SVC, spring viraemia of carp; KHVD, koi herpesvirus disease; EUS, epizootic ulcerative syndrome; WSD, white spot disease) and parasites. Peripheral fish and primary freshwater fish were observed in Jeonryu-ri with cyprinid caught most frequently. Crustaceans were mostly marine species. No positive bands to any of the reportable diseases were produced in any of the fish and crustacean examined in this study by PCR. No trace of Clonorchis sinensis, a liver fluke potential threat to human health, was detected in any of fish samples. However, many fish were infected by metacecaria of other flukes, and other various parasites such as nematode, cestode, copepod, monosite and acanthocephalan. These results suggest that important aquatic organisms in the Han River estuary is not seriously polluted yet. However, it is important to keep monitoring the diseases since the water quality in this region is constantly changing, and devastating influence of infectious diseases is unpredictable. Further, it is required to expand monitoring area toward upstream and increase the number of fish for examination.

A Survey on Current Situation of Computer System Utilization for Poultry Farm Management (양계농가 전산화 실태에 관한 조사 연구)

  • 최연호;이상진;신영수
    • Korean Journal of Poultry Science
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    • v.23 no.4
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    • pp.209-219
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    • 1996
  • This research was carried out to get the basic information on the current situation of personal computer utilization for poultry farm management, and the results obtained could be used in developing of the software system for the poultry farm management. Survey method by interview and mail was adopted to carry out the research, and the total number of data used to statistical analyses was k6 poultry farmers. 1. The ratio of the poultry farmers who had the personal computer(P/C) was about 25%, and the most of the layer farmers from age 30 to 40 who manage the flock size 20,000 or above had the P/C. 2. About 70% of the farmers who had P/C have bought the system after 1993, and 83% of those farmers had the P/C-486 or above level system. They bought the P/C with intent to apply it to farm management(39%) and education for children(31%). 3. About 50% of the farmers who had P/C obtained the relevant knowledge for operating computer system by themselves, and the 33% of the P/C-possessed farmers used P/C for word-processor. 4. About 35% of the farmers who had P/C applied their system to analyse the management analyses, and the sarne ratio of the farmers answered that they didn't apply the system to their farm management. 5. About 25% of the farmers who had P/C used package software, and the company which installed the automated cage system offered the software. 6. Most of the farmers considered the computerization of the farm management positively, and they answered that they will apply P/C to their management actively from now on. 7. About 56% of the farmers didn't think that they had sufficient knowledge for operating computer system, and a quarter of them pointed that the difficulty of the buying software for poultry farm management was the most biggest problem to spread out the computerization in the poultry farm. 8. Most of the poultry farmers didn't buy P/C because they didn't have the relevant knowledge for computer system, but they wanted to learn basic knowledge for computer, and they had the intention to apply computer to their management positively. In order to expand the computerization for poultry farm management, appropriate education system for the farmers from age 30 to 40 who manage the flock size 20,000 or above and the development of the software that could be applied to poultry farm management on the spot efficiently should be made.

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