• 한국약용작물학회지
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• 제13권1호
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• pp.30-34
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• 2005

경남지역 자생 둥글레속 수집종의 유연관계를 규명하여 품종육성을 위한 기초자료로 할용하고자 47개 수집종의 12개 형질을 대상으로 주성분 분석 및 average linkage cluster 방법으로 군을 분류한 결과는 다음과 같다. 1. 주성분 분석에서 줄기형태, 줄기색깔, 엽색, 포의 형태, 화색, 화형 등 12개 주요 형질의 주성분의 고유치에 기여율은 제3주성분까지 분석하여도 전체의 79%정도 설명할 수 있어서 47개 수집종의 분류가 가능함을 알 수 있었다. 2. 47개 수집종의 12개 주요형질을 대상으로 average linkage cluster 방법에 의한 분석치으 거리 0.7을 기준으로 군을 분류한 결과 5개군으로 분류되었다. I군은 둥굴레, II군은 용둥글레, III군은 산둥굴레 및 퉁둥굴레 특성의 중간형태를 각각 지닌 p28과 p29였으며, 제V군은 죽대로 분류되었다. 3. 경남일대 둥굴레의 특성조사와 수집종 분류결과 둥굴레와 죽대는 종간 변이가 거의 없었던 반면, 용둥굴레, 퉁둥굴레와 산둥굴레는 다양한 변이종이 분포하고 있는 것으로 나타났다.

• 한국환경농학회지
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• 제34권3호
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• pp.178-185
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• 2015

본 연구는 봄철 한국 중서부지역 논습지를 중간기착지로 이용하는 도요물떼새류의 취식행동을 확인하여 이들의 서식지 이용 특성을 파악하기 위해 수행되었다. 대상 종은 종달도요, 알락도요, 청다리도요, 흑꼬리도요였으며, 이들은 논습지에서 쉽게 관찰될 뿐 아니라 형태적 차이가 크게 나타난다. 서식지는 봄철 논습지에서 쉽게 확인되는 대표적인 형태인 "갈아엎은 후 물을 댄 필지", "써레질 후 5 cm 이하의 수위를 유지하는 필지", "써레질 후 5 cm 이상의 수위를 유지하는 필지"로 나누었다. 종달도요는 "갈아엎은 후 물을 댄 필지"을 이용하였고 이때 주로 시각과 촉각 모두 이용하는 취식 방법을 선택하였다. 알락도요와 청다리도요는 "써레질 후 5 cm 이하의 수위를 유지하는 필지"에서 많이 관찰되었고 시각을 이용한 취식방법을 주로 선택하였다. 흑꼬리도요는 "써레질 후 5 cm 이상의 수위를 유지하는 필지"를 이용하였고 촉각을 이용한 취식방법을 선택하였다. 본 연구결과를 통해 토양관리와 관개강도에 영향을 받는 수위가 취식지의 접근성과 먹이 발견성에 영향을 미치는 것을 확인 할 수 있었다.

• 동의생리병리학회지
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• 제23권1호
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• pp.214-218
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• 2009

Platycodon grandiflorum is a long-lived herbaceous and one of the very important herbal medicine and foods. P. grandiflorum is called do-ra-ji in Korea. Inter-simple sequence repeats (ISSR) markers were performed in order to analyse the phenetic relationships of four accessions of P. grandiflorum. Wild groups had higher expected diversity, 0.164 for Korean and 0.157 for Chinese accessions than those of cultivated groups, 0.079 for Korea and 0.059 for China. The total genetic diversity in P. grandiflorum was 0.268 across species and the value was lower than average values for species with similar life history traits. The patchy distribution and domestication are proposed as possible factors contributing to low genetic diversity. An assessment of the proportion of diversity within species, HAccession/HSpecies, indicated that about 57.1% the total genetic diversity was among species. Thus, the majority of genetic variation (42.9%) resided within accessions. The estimated Nm (the number of migrants per generation) was very low among four accessions (mean Nm = 0.376). The low estimate of Nm indicated that gene flow was not extensive among four accessions. ISSR01-02 locus can be recognized as an unique locus of Korean groups (wild and cultivated accessions). Thus the locus can be used to distinguish Korean accessions from Chinese accessions. ISSR04-06 locus was found specific to Chinese groups (wild and cultivated accessions) and was not shown in Korean accessions. Although the size of sampling was not large enough for P. grandiflorum, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of accessions.

• Journal of Plant Biotechnology
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• 제39권1호
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• pp.75-80
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• 2012

$Kalopanax$ $pictus$ is a long-lived deciduous perennial tree in the family Araliaceae mainly distributed in the East Asia. In Korea, this species is of ecological and medical importance. Because typical populations of this species are small and distributed in patches, $K.$ $pictus$ has been considered as a narrow habitat species. To understand the genetic diversity and population structure of this species, the sequence variation of the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region was analyzed among 18 different $K.$ $pictus$ populations in the present investigation. The nrDNA ITS sequences of Korean populations investigated in this study showed identical of 616 bp in length, and no any nucleotide variation was found in the entire nrDNA ITS region sequence. This result suggested that the $K.$ $pictus$ populations in Korea might belong to the same isolate, and no mutation was found in the nrDNA ITS region. Compared with other known ITS sequence sources from $K.$ $pictus$ populations, only four variable nucleotide sites were found within the entire ITS region. Very narrow genetic diversity appearing in the population level of $K.$ $pictus$ makes us hypothesize that their relatively isolated habitats. The long-lived traits might be one main reason. However, another probability was that the nr-DNA ITS region might be noneffective in classifying populations of $K.$ $pictus$. Thus, to further understand the phylogenetic relationship of $K.$ $pictus$ populations, more samplings should be performed based on more DNA sequences.

• 한국약용작물학회지
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• 제18권4호
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• pp.266-272
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• 2010

There is a considerable difference in morphological traits between Bokbunja cultivated in Korea (KCB) and Korea native Rubus coreanus, contrary to the conviction that the cultivated Bokbunja is the domestication of R. coreanus. To infer the phylogenetic relationship of KCB with other Rubus species, we compared the chloroplast DNA spacers of KCB with those of several Rubus species including black raspberry, R. occidentalis. The three chloroplast DNA spacers, atpB~rbcL, trnL~trnF, and trnT~trnL, were amplified using the specific primer pairs and converted to Single Strand Conformational Polymorphism (SSCP) markers. The SSCP makers of the chloroplast DNA spacers showed a considerable variation both within and among Rubus species. In the phylogenetic tree generated by the SSCP markers, KCB accessions were located in the same clade with R. occidentalis, but R. coreanus accessions in the different clade. Also, in the phylogenetic tree by the nucleotide sequences of the chloroplast DNA spacer trnL~trnF, KCB located in the same clade with R. occidentalis but not with R. coreanus. These results suggest that the three KCB accessions share higher similarity with R. occidentalis than with R. coreanus in the three chloroplast DNA spacers.

• 한국자원식물학회지
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• 제23권6호
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• pp.541-549
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• 2010

The present study researched morphological characteristics and analyzed the genetic diversity by using RAPD in Calanthe species, native plant in Jeju, Korea. Twenty-six samples were selected by flower color, and 19 horticultural traits were investigated to study morphological characteristics. The C. discolor had the smallest leaf, the length and width of dorsal sepal, lateral sepal, petal, central lip, lateral lip, and flower stalk length were shortest and/or smallest except the spur and ovary length in Calanthe species, but those of Calanthe discolor for. sieboldii (Dence.) Ohwi (Calanthe discolor for. sieboldii) were the largest and/or biggest, and those of variants were the intermediate between C. discolor and C. discolor for. sieboldii, but spur length was the longest in C. discolor, the shortest in C. discolor for. sieboldii, and intermediate in the variants. Ovary length in C. discolor was shortest and C. discolor for. sieboldii and variants were similar with each other. The flower colors of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. discolor for. sieboldii was yellowish; the value of CIE Lab was between 110 and 130. And variants had various colors between 50 and 70 in the value of CIE Lab. After analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of Genetic distance of Calanthe species using RAPD showed that C. discolor and C. discolor for. sieboldii are more distant from each other than variants, and demonstrated the fact that genetic position of variants is between the other two species.

• ALGAE
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• 제33권2호
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• pp.143-156
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• 2018

Dolichospermum is a filamentous and heterocytous cyanobacterium that is one of the commonly occurring phytoplanktons in the Han River of Korea. Morphological observations led to the identification of D. planctonicum-like filaments in seasonal water samples. In the present study, we successfully isolated these filaments using culture methods, and examined its morphology using light and scanning electron microscopy. The morphology of the D. planctonicumlike species differed from that of typical D. planctonicum; it had thin cylindrical-shaped akinetes, which were narrower towards the ends than at the center. This morphology is firstly described in the genus Dolichospermum. In addition, the akinetes in the filament developed solitarily and were distant from the heterocytes. Phylogenetic analysis of the 16S rRNA sequences showed that our Dolichospermum clustered with D. planctonicum and D. circinale, which have coiled trichome. However, phylogenetic analysis of the gene encoding rivulose-1,5-bisphosphate carboxylase (rbcLX) clearly separated our species from other Dolichospermum, forming a unique clade. Additionally, structures of D. planctonicum and D. hangangense strains were different type in Box-B and V3 region. These results demonstrated that the new Dolichospermum species was unique in morphology and molecular traits. Therefore, we propose this to be a new species belonging to genus Dolichospermum with the name Dolichospermum hangangense sp. nov.

• Molecules and Cells
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• 제39권9호
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• pp.692-698
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• 2016

Advances in next generation sequencing (NGS) technologies have enabled population-level studies for many animals to unravel the relationships between genotypic differences and traits of specific populations. The objective of this study was to perform evolutionary analysis of single nucleotide polymorphisms (SNP) in genes of Korean native cattle Hanwoo in comparison to SNP data from four other cattle breeds (Jersey, Simmental, Angus, and Holstein) and four related species (pig, horse, human, and mouse) obtained from public databases through NGS-based resequencing. We analyzed population structures and differentiation levels for the five cattle breeds and estimated species-specific SNPs with their origins and phylogenetic relationships among species. In addition, we identified Hanwoo-specific genes and proteins, and determined distinct changes in protein-protein interactions among five species (cattle, pig, horse, human, mouse) in the STRING network database by additionally considering indirect protein interactions. We found that the Hanwoo population was clearly different from the other four cattle populations. There were Hanwoo-specific genes related to its meat trait. Protein interaction rewiring analysis also confirmed that there were Hanwoo-specific protein-protein interactions that might have contributed to its unique meat quality.

• 생태와환경
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• 제44권1호
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• pp.42-57
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• 2011

We described the above-ground plant species composition and measured a range of soil physico-chemical properties and the composition and size of the soil seed bank in the remnant natural vegetations on the flood plains of the Han River within Seoul, South Korea. We used analysis of variance and multivariate analyses to analyse the data and S${\o}$rensen's similarity index to compare the composition of the vegetation and seed banks. The soils were circum-neutral and composed of mainly sand and silt fractions with a very limited clay component; a gradient based on sand/clay proportions was identified. The soil seed banks varied markedly between- and within-sites and had much greater species diversity than the above-ground vegetation. Two of the major dominants in the vegetation (Miscanthus saccariflorus and Phragmites australis) were found at very low densities in the seed bank. The site differences appeared to be correlated with the sand-clay gradient, suggesting that the soil properties differentially affected seed inputs into the soil, or that the processes than controlled sediment deposition during floods was also important in differentially affecting seed deposition. Lastly, there was relatively little similarity between the vegetation, dominated mainly by perennials, and the seed bank which contained a relatively large proportion of annuals and biennials. This result suggests that after disturbance caused by flooding there is the potential for many other species to colonize. This may impinge on the regeneration potential of the sites and cause concern for the future conservation of these important remnants of natural vegetation.

• 식물분류학회지
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• 제49권4호
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• pp.324-333
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• 2019

Neutral genetic diversity found in plant species usually leaves an indelible footprint of historical events. Korea's main mountain range (referred to as the Baekdudaegan [BDDG]), is known to have served as a glacial refugium primarily for the boreal and temperate flora of northeastern Asia. In addition, life-history traits (life forms, geographic range, and breeding systems) influence the within- and among-population genetic diversity of seed plant species. For example, selfing species harbor significantly less within-population genetic variation than that of predominantly outcrossers. A previous study of two Liparis species (L. makinoana and L. kumokiri) emphasizes the role of the abovementioned factors shaping the levels of genetic diversity. Liparis makinoana, mainly occurring on the BDDG and self-incompatible, harbors high levels of within-population genetic diversity (expected heterozygosity, H_eP = 0.319), whereas there is no allozyme variation (H_eP = 0.000) in L. kumokiri, which is self-compatible and mainly occurs in lowland hilly areas. To determine if this trend is also found in other congeners, we sampled five populations of L. krameri from the southern part of the Korean Peninsula and investigated the allozyme-based genetic diversity at 15 putative loci. The somewhat intermediate levels of within-population genetic variation (H_eP = 0.145) found in L. krameri are most likely due to its occurrence in mountainous areas that, despite being outside of the main ridge of the BDDG, still served as refugia, and a self-incompatible breeding system. Management strategies are suggested for L. krameri and L. makinoana based on the levels and distribution of genetic diversity and inbreeding.