• ALGAE
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• v.31 no.1
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• pp.1-16
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• 2016

Paralia species have been frequently reported as P. sulcata in Korea, despite the species diversity within the genus. To understand the species diversity of Paralia in Korea, we collected phytoplankton samples at 79 sites from April 2006 to April 2015. Five Paralia species, P. fenestrata, P. guyana, P. marina, P. cf. obscura, and P. sulcata, were observed during this study, and we described their fine structure in terms of quantitative and qualitative morphological characteristics. To provide additional criteria to identify Paralia species more clearly, we morphometrically analysed four quantitative characteristics on valve diameter: pervalvar axis / diameter, internal linking spines / diameter, marginal linking spines / diameter, and fenestrae/diameter using non-metric multidimensional scaling (MDS). MDS analysis distinguished four Paralia species: P. guyana, P. marina, P. cf. obscura, and P. sulcata, with the exception of P. fenestrata. This new approach in using morphometric analysis is useful for the accurate identification of Paralia species.

• Journal of Species Research
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• v.9 no.3
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• pp.191-197
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• 2020

Species belonging to the genus Tryblionella are important members of the diatom assemblages of benthic environments such as tidal flats. Their proper identification is thus of great importance in terms of taxonomy, ecology, as well as environmental sciences. However, many species of the genus have been neglected and/or misidentified during diatom studies of Korean tidal flats possibly due to the small size of the species. Lack of proper references for the identification of the species is also noted. To better understand the diversity of Tryblionella species, sediment samples were taken from various tidal flats of the Yellow Sea. Light microscope (LM) and scanning electron microscope (SEM) observations have shown the presence of Tryblionella adducta and T. hyalina, which have not been previously reported from Korean tidal flats. The former was found mostly from sand flats and the latter from mudflats. It is expected that the present study would contribute to a better understanding of the diversity and ecology of benthic diatoms of the Korean tidal flats.

• Mycobiology
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• v.50 no.4
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• pp.219-230
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• 2022

Agaricales species form pileate-stipitate fruiting bodies and play important roles in maintaining the terrestrial ecosystem as decomposers, symbionts, and pathogens. Approximately 23,000 Agaricales species have been known worldwide, and 937 species have been recorded in the Republic of Korea. However, most of them were identified solely based on morphological characteristics that often led to misidentifications. The specimens collected from 2018 to 2020 in the Republic of Korea were identified based on phylogenetic analysis of the internal transcribed spacer (ITS) sequences. Their identities were confirmed by microscopic characteristics. As a result, 14 Agaricales species were discovered for the first time in the Republic of Korea. They belonged to nine genera: Agaricus, Calocybe, Cortinarius, Hygrocybe, Inocybe, Lepista, Leucoagaricus, Marasmius, and Psathyrella. Detailed macroscopic and microscopic descriptions were provided to help distinguish these species. The morphological and molecular data provided in this study will serve as reliable references for the identification of Agaricales species.

• Journal of the Korea Furniture Society
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• v.22 no.2
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• pp.132-139
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• 2011

Wood-anatomical studies on vines, one of the main materials for fork craftworks used in the past, were insufficient compared to those for trees. Therefore, it is important to identify the species of vines in order to preserve the vinery craftwork relics. In this study, the species of 31 samples (30 traditional baskets and one sculpture relic) obtained from Korea were identified. Twenty-two baskets among 30 baskets were identified as Cocculus trilobus DC, a perennial vine which has flexible xylem and thick fibers in phloem. The other baskets were identified as $Lespedeza$ spp., a shrub with tough xylem. The sculpture part was $Actinidia$ $arguta$ Planch., a perennial vine with chambered piths.

• Journal of the Korea Furniture Society
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• v.22 no.2
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• pp.126-131
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• 2011

The purpose of this study were to analyze the species and tree-ring dates of coffin woods excavated at Yerim site in Yesan, Chungnam, Korea. We sampled 12 pieces of woods from two coffins. The species of all coffin woods were identified as red pine group, most likely, $Pinus$ $densiflora$. The last rings of both coffins were dated A.D. 1557 and A.D. 1601, respectively. The tree-ring dates indicated that the coffins were made in the late 16th and early 17th century.

• Journal of the Korean Wood Science and Technology
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• v.24 no.3
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• pp.80-89
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• 1996

Tripitaka Koreana was made during Koryo Dynasty from 1236 to 1251 A.D. Buddhist scriptures were engraved on 81.340 wooden plates. This study was made to identify species in these archaeological wooden plates using light and scanning electron microscopes. The results were as follows more than 62% of whole specimens investigated was Prunus sp., which was used in wooden plate 64% and wooden plate edge 56%, Pyrus sp. was used 13% of whole specimens and 31% of wooden plates. Therefore, 75% of whole Tripitaka Koreana was made by these two species. Acer sp., Betula sp., Machilus sp., Cornus sp., Daphnilhyllum sp., Prunus sp. were also identified but extreamly rare. Especially Machilus sp. and Daphnilhyllum sp. originally distributed in subtropical zone of west and south coast in Korea were identified, suggested strongly another engraving place of Tripitaka Koreana, Namhae bunsadogam.

• Korean journal of applied entomology
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• v.51 no.1
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• pp.73-77
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• 2012

Two pupillarial species, $Cryptoparlatorea$ $leucaspis$ Lindinger and $Fiorinia$ $japonica$ Kuwana, are recorded for the first time in Korea. Both species were found on coniferous trees, $Cryptomeria$ $japonica$ and $Torreya$ $nucifera$. Also an identification key, brief diagnoses, and photographs of Korean pupillarial species are provided to assist in their identification.

• Animal Systematics, Evolution and Diversity
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• v.36 no.3
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• pp.216-221
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• 2020

Parrots are common targets for illegal trade because of their beauty and high price. Accurate identification is necessary for the prevention of illegal trade and conservation of parrots. In the present study, mitochondrial markers of cytochrome b (CYTB) gene were used to identify parrot species from Korean zoos. Totally, 27 samples were collected from Seoul Zoo, Cheongju Zoo, and Uchi Zoo. After collection, total DNA of samples was extracted and used for PCR amplification. CYTB fragments were sequenced from all samples examined. The obtained sequences were used for GenBank blast, distance estimation, and phylogenetic analysis. All species were identified using CYTB sequences that determined 27 samples belong to 13 species in 7 genera, and 3 families. Our finding demonstrated the usefulness of CYTB sequences for identifying parrot species in Korean zoos.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.464-475
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• 2023

In this study, based on an analysis of two DNA barcode markers (cytochrome c oxidase subunit I and cytochrome b genes), we performed species identification and monitored labeling compliance for 50 commercial pufferfish products sold in on-line markets in Korea. Using these barcode sequences as a query for species identification and phylogenetic analysis, we screened the GenBank database. A total of seven pufferfish species (Takifugu chinensis, T. pseudommus, T. xanthopterus, T. alboplumbeus, T. porphyreus, T. vermicularis, and Lagocephalus cheesemanii) were identified and we detected 35 products (70%) that were non-compliant with the corresponding label information. Moreover, the labels on 12 commercial products contained only the general common name (i.e., pufferfish), although not the scientific or Korean names for the 21 edible pufferfish species. Furthermore, the proportion of mislabeled highly processed products (n = 9, 81.8%) was higher than that of simply processed products (n = 26, 66.7%). With respect to the country of origin, the percentage of mislabeled Chinese products (n = 8, 80%) was higher than that of Korean products (n = 26, 66.7%). In addition, the market and dialect names of different pufferfish species were labeled only as Jolbok or Milbok, whereas two non-edible pufferfish species (T. vermicularis and T. pseudommus) were used in six commercial pufferfish products described as JolboK and Gumbok on their labels, which could be attributable to the complex classification system used for pufferfish. These monitoring results highlight the necessity to develop genetic methods that can be used to identify the 21 edible pufferfish species, as well as the need for regulatory monitoring of commercial pufferfish products.