• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3629-3634
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• 2013

In this study, an improved method for the quantitative analysis of ginkgolic acids (GAs) in Ginkgo biloba leaf extract was developed. The samples were extracted with a mixture of chloroform and 50 % ethanol, after which the chloroform extract was dried and reconstituted in methanol. GAs with 13:0, 15:1, and 17:1 in the extract were successfully separated within 40 min and determined with high throughput performance using an online column-switching HPLC method using an SP column C8 SG80 ($4.6{\times}150mm$, $5{\mu}m$) and a Cadenza 5CD C18 column ($4.6{\times}150mm$, $3{\mu}m$). The developed HPLC method was validated for Ginkgo biloba leaf extract. The validation parameters were specificity, linearity, precision, accuracy, and limits of detection and quantitation (LODs and LOQs, respectively). It was found that all of the calibration curves showed good linearity ($r^2$ > 0.9993) within the tested ranges. The LODs and LOQs were all lower than $0.04{\mu}g/mL$. The established method was found to be simple, rapid, and high throughput for the quantitative analysis of GAs in ten commercial Ginkgo biloba leaf extract and dietary supplements. The samples were also analyzed in LC-electrospray ionization (ESI) tandem mass spectrometry (MS/MS) - multiple-ion reaction monitoring (MRM) mode to confirm the identification results that were obtained by the column switching HPLC-DAD method. The developed method is considered to be suitable for the routine quality control and safety assurance of Ginkgo biloba leaf extract.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.5
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• pp.175-182
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• 2010

The paper presents a novel algorithm for face and iris detection with the application for driver iris monitoring. The proposed algorithm consists of the following major steps: Skin-color segmentation, facial features segmentation, and iris positioning. For the skin-segmentation we applied a multi-layer perceptron to approximate the statistical probability of certain skin-colors, and filter out those with low probabilities. The next step segments the face region into the following categories: eye, mouth, eye brow, and remaining facial regions. For this purpose we propose a novel segmentation technique based on estimation of facial class probability density functions (PDF). Each facial class PDF is estimated on the basis of salient features extracted from a corresponding facial image region. Then pixels are classified according to the highest probability selected from four estimated PDFs. The final step applies the circular Hough transform to the detected eye regions to extract the position and radius of the iris. We tested our system on two data sets. The first one is obtained from the Web and contains faces under different illuminations. The second dataset was collected by us. It contains images obtained from video sequences recorded by a CCD camera while a driver was driving a car. The experimental results are presented, showing high detection rates.

• The Korean Journal of Community Living Science
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• v.15 no.2
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• pp.167-177
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• 2004

The number of foodborne salmonellosis was estimated by using microbial risk assessment(MRA) methodology and the possibility of application was studied through comparison with previous results. The contamination levels of Salmonella sp. were estimated by using published domestic studies(1997∼2000) and monitoring data (1999∼2001) from food-safety related institutes. Data on food consumption came from the 2001 National Health and Nutrition Survey, and dose-response models from studies in other countries. Simulation results showed that there were 753,368 cases of salmonellosis in Korea in 1 year, which is about 115 times that reported in previous years and lower than the WHO's estimation increase. From these results, microbial risk assessment is likely to be available for estimation of the number of foodborne illnesses and determination of the order of priority in food-safety management. Butthe verification methods are not established and most of the data on contamination levels of foodborne bacteria, food consumption, and dose-response relationships have not been established. In addition, the actual conditions of circulation, storage and cooking must be studied further.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.2
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• pp.116-124
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• 2011

In this paper, We implemented vessel USN middleware by server-side method considering characteristics of ocean environment. We designed multiple query process module in order to efficient process multidimensional sensor stream data and proposed optimized query plan using Mjoin query and hash table. This paper proposed method that context aware of vessel and manage considering characteristics of ocean. We decided to risk context using SVM algorithm in context awareness management module. As a result, we obtained about 87.5% average accuracy for fire case and about 85.1% average accuracy for vessel risk case by input 5,000 data sets and implemented vessel USN monitoring system.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.4
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• pp.32-40
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• 2008

Since vision-based surveillance system uses a conventional camera which has a narrow field of view, it is difficult to apply it into the environment whose the ceiling is low and the monitoring area is wide. To overcome this problem, the method of increasing the number of camera causes the increase of the cost and the difficulties of camera set-up For these problems, we propose a new surveillance system based on multiple fisheye cameras which have 180 degree field of view. The proposed method handles occlusions using the homography relation between the multiple fisheye cameras. In the experiment, four fisheye cameras were set up within the area of $17{\times}14m$ at height of 2.5 m and five people wandered and crossed with one another within this area. The detection rates of the proposed system was 83.0% while that of a single camera was 46.1%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.spc1
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• pp.132-141
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• 2006

Artificial structures have been designed as pilot structures to promote the creation and restoration of tidal flats. However, little information is available as to whether such artificial construction affects the macrobenthic community structure. We monitored the variation of the macrobenthic community structure and species composition near natural and artificial structures (seaweed and a timber fence) on the tidal flats near the Iwon Dike, Korea. In total, 137 macrobenthic species were recorded during this study, predominantly crustaceans (47%), polychaetes (18%), and molluscs (27%). Polychaetes comprised over 50% of the total density, followed by gastropods (38%) and crustaceans (11%). Macrobenthic species composition in the artificial and natural areas, was initially similar, but it differed after 7 months. The gastropod Umbonium thomasi, the most dominant species, was present at both sites in the first month after the start of the experiment, but disappeared at the artificial sites within 7 months, suggesting disturbance by the environmental factors. The number of species and diversity (H') varied significantly within sites at the beginning of the experiment, but no difference was observed after 7 months. Multivariate analysis (multidimensional scaling) revealed significant differences in community structure between the artificial and the natural areas from 7 months after the start of the experiment, except from 18 to 21 months. The community structures were mainly influenced by U. thomasi. Community structure at the artificial sites was affected by environmental variables, such as carbon, COD/IL sulfide, loss of ignition, kurtosis and silt, which changed over time. We observed no significant correlations between environmental variables and the dominant species, except in the case of Spio sp. and Macrophthalmus dilatatus, suggesting that the biological interactions and temporary disturbances such as typhoon, as well as the effects of artificial structures may also be important regulating factors in this system.

• The Journal of Korean Physical Therapy
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• v.21 no.2
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• pp.109-116
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• 2009

Purpose: $17\beta$-estradiol is the most active endogenous estrogen, which is related to favorable changes in the plasma lipid profile, to relaxation of the coronary vessels, and to a decrease in platelet aggregation and vascular smooth muscle cell migration. However, although the beneficial effect of estrogens on plasma lipoproteins (ie, lowering low-density lipoprotein and increasing high-density lipoprotein cholesterol) contributes to cardiovascular protection, it does not fully account for the protective effect, particularly in the application of physical therapy, including low frequency electrical stimulation. Methods: The aim of this study was to demonstrate the inhibition of stressors, such as endothelin-1 (ET-1), serotonin (5-hydroxytryptamine, 5-HT), prostaglandin $F2\alpha$ ($PGF2\alpha$), and a protein kinase C (PKC) activator 12-deoxyphorbol 13-isobutyrate (DPB), induced isometric tension by $17\beta$-estradiol in vascular smooth muscle strips, respectively. In addition, the effects of low frequency electrical stimulation at the meridian points (CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52) on the indirect antihypertensive effect were examined by monitoring the changes in the serum $17\beta$-estradiol concentration in healthy volunteers. Results: Isometric tension analysis showed that the responses of inhibited tension by $17\beta$-estradiol were similar to the same stressors in rat aortic smooth muscle strips. Furthermore, although the continued amplitude modulation (AM) type of electrical stimulation was not increased significantly by electrical stimulation, the current of the frequency modulation (FM) type of low frequency electrical stimulation increased the serum $17\beta$-estradiol concentration in normal volunteers. Conclusion: These results, in part, suggest that $17\beta$-estradiol has the capacity to supress stressor-induced muscle tension, and electrical stimulation, particularly current of the FM type, has a modulatory effect on the sex steroid hormones, particularly $17\beta$-estradiol, in healthy volunteers.

• 한국지구물리탐사학회:학술대회논문집
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• 2008.10a
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• pp.7-11
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• 2008

기상청은 2008년 현재 전국에 107개의 디지털 지진관측망을 운영하고 있다. 또한 지진관측 유관기관인 한국지질자원연구원, 한국원자력안전기술원, 한전전력연구원에서 각 기관의 목적에 맞게 지진관측소를 운영하고 있으며, 이들 지진관측자료의 공유를 목적으로 실시간으로 통합하는 통합지진과측망(KISS)을 2000년에 구축하여 지진분석에 쓰이고 있다. 한반도의 지진발생 현황은 1978년부터 2007년까지 776회로 약 26회/년 정도가 발생하였다. 최근 중국의 쓰촨성과 일본 이와테 지진으로 지진 및 지진예지에 대한 국민적 관심이 대두되고 있다. 한반도에서도 큰 규모의 지진이 발생할 가능성에 대비해 기상청에서는 지진관측망과 지진예지 목적의 지구물리관측망을 구축 중에 있다. 지구물리관측망은 INTERMAGNET에서 등록할 수 있는 수준의 관측소를 목표로 인위적인 자기장의 교란이 적은 지질학적인 요소들을 고려한 후보지 선정을 위하여 지구자기업무에 관한 기획연구(서만철 2007)를 수행 하였다. 그 결과 국가 중심 지구자기관측소의 위치는 충남 공주시와 청양군 사이에 있는 칠갑산 지역이 가장 좋은 후보지라고 제안하여 청양지역을 주변으로 국유림 및 군유지를 조사하여 충남 청양군 장평면 화산리 산 36-2번지에 후보지를 선정하여 최적의 입지 조건을 검증하기 위해 인천교육대학교의 이휘순 교수 MT탐사를 수행 주변의 배경잡음을 측정하여 양호한 조건을 갖춘 것으로 확인되었다. 지구자기관측소에는 Fluxgate Magnetometer 1대, Total field Magnetometer 1대, Theodolite 1대, SP(Self Potential) Monitoring system 1대와 장비들을 보호 할 수 있는 관측소가 설치될 예정이다

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.677-683
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• 2017

The Korean aquaculture industry was generally began in the 1970s and has gradually increased. Now, the number of households is about 7,068 and the scale of an aquaculture farm is about 248,014 ha; the value of all production in the industry is estimated to be about 1602.2 billion won. The aquaculture industry is very valuable and important for future food resources. However, the aquaculture industry was damaged by several marine ecological disturbance causing organisms. The Ascdiella aspersa colonized on the shell of scallop and then scallops were detached from rope. The patterns of damage in the aquacultures were observed in Tongyeong (oyster), Geojedo (oyster), and Gangneung (sea squirt) in June, 2017, as well as in Tongyeong (oyster) in November, 2017 by SCUBA divers. The species Halichondria bowerbanki, Bugula neritina, Mytilus galloprovincialis, Balanus amphitrite, Ascidiella aspersa, Ciona intestinalis, Didemnum sp, Styela plicata in Tongyeong, M. galloprovincialis, A. aspersa, C. intestinalis, D. vexillum, S. plicata in Geojedo, and M. galloprovincialis in Gangneung were all usually found in their farms. The marine ecological disturbance causing organisms gave rise to a reduced number of aquaculture products.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.397-402
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• 2009

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.