• Mass Spectrometry Letters
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• 제12권3호
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• pp.85-92
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• 2021

The therapeutic antibody drug market has experienced explosive growth as mAbs become the main therapeutic modality for a variety of diseases. Characterization of glycosylation that directly affects the efficacy and safety of therapeutic monoclonal antibodies (mAbs) is critical for therapeutics development, bioprocess system optimization, lot release, and comparability evaluation. The LC/MS approach has been widely used to structurally characterize mAbs, and recently attempts have been made to obtain comprehensive information on the primary structure and post-translational modifications (PTMs) of mAbs through intact protein analysis. In this study, we performed state-of-the-art LC/MS based intact protein analysis to readily identify and characterize glycoforms of various mAbs. Different glycoforms of mAbs produced in different expression cell lines including CHO, SP2/0 and HEK cells were monitored and compared. In addition, the comparability of protein molecular weight, glycoform pattern, and relative abundances of glycoforms between the commercialized trastuzumab biosimilar and the original product was determined in detail using the given platform. Intact mAb analysis allowed us to gain insight into the overall mAb structure, including the complexity and diversity of glycosylation. Furthermore, our analytical platform with high reproducibility is expected to be widely used for biopharmaceutical characterization required at all stages of drug development and manufacturing.

• ALGAE
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• 제19권2호
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• pp.145-148
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• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

• 대한전자공학회논문지SP
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• 제48권3호
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• pp.112-118
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• 2011

선박은 위급상황에 노출될 경우 육상의 교통수단에 비해 해양 환경이라는 제약성 때문에 그 위험성이 더 크다. 따라서 위험 요소를 사전에 발견하고 먼저 조치를 취하는 것이 매우 중요하다. 이 논문에서는 선박에 가해지는 위험 요소를 지속적으로 감시하고 상태를 파악할 수 있는 상태감시시스템을 제안하였으며 이를 위해 선박 내에 화재 센서 모듈, 기울기 센서와 비틀림 센서를 설치하여 선박의 안전 상황을 판가름하는 기초가 되는 센서 데이터를 수집하였다. 획득한 센서 데이터는 역전파 신경망을 설계하여 분석하였으며 분석된 데이터를 토대로 판단한 선박 상태 정보는 승무원의 개인 단말기로 송신되어 이동 상황에서도 선박의 상태를 실시간으로 점검할 수 있다. 상황인식 실험 결과 연출된 화재 상황에서 약 95%의 정확도를 보였고 선체의 충격 위험 요소에 대해서 약 89%의 정확도를 보였다.

• 한국환경과학회지
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• 제25권4호
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• pp.459-472
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• 2016

We assessed the distribution of benthic macroinvertebrate at four surveying sites in Upo Wetland, a Ramsar site and the largest wetland in Korea, from February 2006 to November 2013. A long-term ecological monitoring was done monthly by using quantitative sampling method with dip net for analyzing the community change and correlation between the biodiversity and the environmental factors because environmental factors have a decisive effect on the community structure of the benthic macroinvertebrates. Total samples from the Upo Wetland area were classified into 3 phyla, 6 classes, 17 orders, 68 families, 176 species, and 25,720 individuals. Among the 176 species, 62 of the species had not been previously reported in this area. Asellus sp., Diplonychus esakii, and Gyraulus chinensis were the most common species in Upo Wetland. The change patterns of annual species diversity and species richness were calculated by using the average number of monthly occurring species and individuals. Diversity index increased from March to May and decreased after that showing lower diversity indices in July and August. It increased again in September and in October. Richness index showed similar tendency and fell around February and July, followed by a rising tendency around May and October. Correlation and regression analyses were performed with the change of biodiversity and each environmental factor. We found that water temperature have very significant positive correlation with species diversity in spring, and have significant indices in autumn and winter. On the other hand, precipitation showed a significantly negative correlation value in summer and autumn suggesting it has an effect on the community structure of benthic macroinvertebrates.

• KIEAE Journal
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• 제17권1호
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• pp.77-82
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• 2017

In power supply systems for urban areas, issues such as a progressive tax have escalated recently. In this regard, photovoltaic power generation, which is appraised as an alternative power generation system, is drawing attention increasingly for its high stability and applicability to existing infrastructure. This study assessed the realistic feasibility of photovoltaic power generation and also analyzed the economic benefits expected when it is linked with green roof, which is likely to promote ecological functions in urban areas, based on the Seoul solar map, RPS, and actual monitoring data. The economics analysis of 30kW photovoltaic power generation applied with the monthly average horizontal solar radiation of six grades in the Seoul solar map showed that positive NPV was up to grade 4, while grade 5 or poorer showed negative NPV and indicated that it is difficult to assure appropriate feasibility. Compared with non-afforestation, when green roof was applied, monthly average power improvement efficiency was 7.2% at highest and 3.7% at lowest based on yearly actual monitoring data. The annual average was 5.3%, and the efficiency was high relatively in summer, including September and November. As for the economic benefits expected when 30kw photovoltaic power generation is combined with green roof based on the average horizontal solar radiation of grade 1 in the Seoul solar map, SP has improved 0.2 years to 7.4 years, and EP has improved 0.5 years to 8.3 years.

• 대한전자공학회논문지SP
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• 제47권5호
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• pp.81-88
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• 2010

본 논문에서는 실제 방송 환경에 적용 가능한 방송용 음원 모니터링 시스템을 구축하기 위한 사전연구로 방송 오디오 신호로부터 음악신호 구간을 자동으로 검출할 수 있는 시스템을 제안하였다. 음악구간과 비음악구간의 구분을 위한 특징으로는 사람의 음성 발화 특성을 반영하여 에너지 표준편차와 log 에너지 표준편차 그리고 log 에너지 평균 등 3개의 간단한 시간영역 특징들을 사용하였으며 최종 음악신호 구간 판별은 각 에너지 한계값(threshold)을 이용한 Rule-base 분류를 기반으로 하였다. 실제 FM 라디오 방송 신호를 24시간 녹음하여 진행한 모의실험에서 음악구간 인식률은 96%, 비-음악구간 인식률은 87%를 나타내어 방송용 음원 모니터링 시스템의 전처리기로 손색이 없음을 확인할 수 있었다.

• 생태와환경
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• 제47권4호
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• pp.273-281
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• 2014

영산강에서 동계 대증식을 유발하는 Stephanodiscus속의 출현기간과 종 분류를 위해 2012년 12월부터 2013 년 4월까지 주 단위로 21회의 모니터링을 실시하였다. 동계 영산강에서는 규조류가 절대적으로 우세한 군집을 보였으며, 규조강 중 원형규조 Aulacoseira, Cyclotella, Stephanodiscus속이 주요 출현종으로 동정되었다. 이 중 Stephanodiscus spp.는 극우점 규조류로서 12월 초에 출현하기 시작하여 1월에 최대 개체수를 나타냈고, 3월까지 높은 분포를 보였다. 기온이 증가하기 시작하는 4월부터 개체수가 급격히 감소한 반면 녹조강, 남조강, 은편모조강 등이 증가하는 경향을 나타냈다. 우점종은 거의 모든 조사시기에 원형 중심목 규조류 (S. hantzschii)가 우점 및 차우점종으로 동정되었으며, 3월 말과 4월 중에는 남조강인 Anabaena sp.와 은편모조강인 Cryptomonas sp.가 우점 및 차우점종으로 출현함으로써 계절 변화에 따른 종 천이가 관찰되었다. 조사시기에 출현한 원형 중심목 규조류의 명확한 종 분류를 위해 전자현미경을 통해 동정한 결과, Stephanodiscus속 내의 S. hantzschii가 극우점한 가운데 부분적으로 S. minutulus와 S. parvus 동정되었으며, 그밖에 원형 중심목 규조류에는 Cyclostephanos속의 C. invisitatus와 Cyclotella속의 C. meneghiniana, Discostella속의 D. pseudostelligera, D. woltereckii가 동정되었다.

• 대한한의학방제학회지
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• 제30권4호
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• pp.241-248
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• 2022

Objective : The purpose of this trial is to observe the preliminary effects of Salvia plebeia (SP) extract on quality of life in patients with solid cancer. Methods : This is a prospective, open-label, single-arm, and single-dose clinical trial. Twenty participants who have been diagnosed with solid cancer between the ages of 20 and 65 will be included. All participants will be administered SP granules for 12 weeks. Data will be collected at 4, 8, and 12 weeks after enrollment. The primary outcome is quality of life, using the Korean version of the Functional Assessment Cancer Therapy-General questionnaire. Secondary outcomes include tumor markers in blood tests for each cancer type, soluble programmed death-ligand 1, the percentage of natural killer cells among lymphocytes, ratio of T-helper and T-suppressor cells, ratio of total T, T-helper, T-suppressor, and B cells in lymphocytes, level of C-reactive protein, and tumor size via radiology examination. Safety will be assessed by clinical laboratory tests and monitoring of adverse events. Discussion : This study aims to observe the effects of an oral administration of SP preparations in patients with solid cancer on changes in quality of life and an improvement in immune function. It is expected to provide objective evidence of the effect and safety of SP for patients with solid cancer. Trial registration: KCT0007315 (Clinical Research Information Service)

• Animal Systematics, Evolution and Diversity
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• 제36권1호
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• pp.35-40
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• 2020

From a result of surveys on the biodiversity monitoring for moths in Korea, six species of micro-moths that were never known from Korea are reported here for the first time from this country. They are: Udea pseudocrocealis(South, 1901) of the family Crambidae, which has been known as endemic to Japan; Epinotia albiguttata (Oku, 1974) of the family Tortricidae, which was known belonging to Hikagehamakia Oku, 1974; and four species of the family Gelechiidae: Protoparachronistis concolor Omelko, 1986 and P. discedens Omelko, 1986, which were described from Primorisk, Russian Far East; Thiotricha pancratiastis Meyrick, 1921, which is known from Isl. Jejudo, and Thiotricha sp. which is being described in a separate paper. Here we provide images of adults and genitalia for the unrecorded species with a brief diagnosis.

• 한국어병학회지
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• 제33권1호
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• pp.1-6
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• 2020

In this study, carp edemavirus disease and herpesviral hematopoietic necrosis were examined in carp (Cyprinus carpio) and crucian carp (Carassius auratus) imported from China. From August 2016 to May 2017, 100 carp and 300 crucian carp were examined. There were no clinical symptoms in all the tested fish, and no internal or external parasitic infections were identified except for the detection of Argulus sp. in one leather carp. Carp edema virus disease was examined by PCR in C. carpio, but all were negative. In C. auratus, herpesviral hematopoietic necrosis was tested by PCR and 4 out of 300 were positive. The results of this study are expected to be useful as basic data for conducting risk analysis on the relevant organisms and counterpart countries or for designating new pathogens for quarantine.