• Journal of the Korean Wood Science and Technology
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• 제23권1호
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• pp.13-20
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• 1995

This study was carried out to investigate the characterization of lignins from waste liquors in SP, KP, ASAM, and AS from Pinus densiflora, Quercus mongolica, and Betula ermanii. Spectroscopic study was applied to examine the lignins separated from different pulping process. Lignin contents in waste liqours increased in order of AS, ASAM, KP, and SP. UV spectra of three types of lignin except AS lignin showed similar pattern. IR spectra of AS lignin showed strong C=O absorptions in the range from 1730 to 1750$cm^{-1}$, where as those of KP, SP, and ASAM showed weak stretch in this region. NMR spectra of AS lignin showed strong characteristic chemical shifts of acetoxyl groups of acetylated aliphatic and aromatic hydroxyl groups at 2.0~2.5 ppm. Molecular weight of ASAM lignin from Pinus densiflora determined and found number average molecular weight 1,199, weight average molecular weight 5,458. z average molecular weight 17,242, and viscosity average molecular weight 5,457. It is considered from the results based on spectroscopic study of lignin that waste liquors (in SP, KP, ASAM and AS) from Pinus densiflora, Quercus mongolica, and Betula ermanii can be used for lignin utilization.

• 생명과학회지
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• 제12권4호
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• pp.392-398
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• 2002

Lignocellulose 분해 이용을 위해 토양 등에서 lignin분해 능력이 우수한 세균을 분리 및 동정함으로서 방향족 화합물의 이용을 위한 생물전환의 기초를 확보하고자하였다. 토양 등의 시료로부터 38주의 리그닌 분해 균주를 분리하였다. 분리된 LG2를 공시 균주로 선정하여 형태학적, 배양학적 및 생리학적 특성을 조사한 결과 Pseudomonas sp. LG2로 명명하였다. 이 균주는 리그닌을 분해 할 수 있었으며, 리그닌 함유 배지에 배양하여 배양 분해 산물을 HPLC로 분석한 결과 다수의 방향족 화합물을 생산하였다 Poylacrylamide gel활성 분석에 의해서 3종류로 구성된 peroxidase가 조사되었다.

• 현장농수산연구지
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• 제5권1호
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• pp.3-7
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• 2003

흰목이버섯 발생부위에 흰목이 균과 공생하는 Hypoxion sp.의 톱밥 감소율과 lignin, holocellulose 감소율을 조사한 결과 흰목이균과 Hypoxylon sp. 혼합처리균, Hypoxylon sp., 흰목이균 순으로 높았다. 공시균의 균사 배양중 lignin 감소율을 시험한 결과, 흰목이균은 20일후에 0.4%, 100일~120일 후에는 1.9%이며, 혼합균은 20일 후에 7.8%, 120일후에는 26.8%, Hypoxylon sp.은 20일후에 6.9%, 120일 후에는 25.9%이었다. 공시균의 균사 배양중 holocellulose 감소율을 시험한 결과, 흰목이균은 20일 후에 0.08%, 120일 후에는 0.7%, 혼합균은 20일 후에 0.8%, 120일 후에 12%, Hypoxylon sp.은 20일 후에 0.7%, 120일 후에 11.6%이었다.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1765-1773
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• 2016

Wastewater containing kraft lignin (KL) discharged from pulp and paper industries could cause serious environmental contamination. Appropriate effluent treatment is required to reduce the pollution. Investigations on anaerobic bacteria capable of degrading KL are beneficial to both lignin removal and biofuel regeneration from the effluent. In this paper, an anaerobic strain capable of degrading KL was isolated from the sludge of a pulp and paper mill and identified as Dysgonomonas sp. WJDL-Y1 by 16S rRNA analysis. Optimum conditions for KL degradation by strain WJDL-Y1 were obtained at initial pH of 6.8, C:N ratio of 6 and temperature of 33℃, based on statistical analyses by response surface methodology. For a 1.2 g/l KL solution, a COD removal rate of 20.7% concomitant with biomass increase of 17.6% was achieved after 4 days of incubation under the optimum conditions. After the treatment by strain WJDL-Y1, KL was modified and degraded.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.211-214
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• 2000

The roles of lignin peroxidase, manganese peroxidase, and laccase were inverstigated in the biodegration of pentachlorphenol (PCP) by several which rot fungi. The disappearance of pentachlorophenol from cultures of wild type strains, P. chrysosporium, Trametes sp. and of pentachlorophenol from cultures of wild type strains, P. cheysocporium, Trametes sp. and Pleurotus ap., was observed. The activities of mangnese peroxidase and laccase was detected in Trametes sp. and pleurotus sp. cultures. However, the activities showed that PCP was degraded under ligninolytic as well as nonligninoytic condicationg that lignin peroxidase, manganese peroxidase, and laccase are not essential in the biodegradation of PCP by white rot fungi.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2002년도 춘계공동학술대회 및 심포지움 초록집
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• pp.58-58
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• 2002

• Applied Biological Chemistry
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• 제47권3호
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• pp.287-292
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• 2004

Phellinus igniarius 26005에 의한 lignin peroxidase의 생산 조건의 최적화를 검토하였다. Lignin peroxidase는 진탕배양보다는 정치배양에서 생산성이 높았으며 최적 생산배지는 malt extract 1 g, yeast extract 0.4 g, glucose 0.4 g, 증류수 100 ml이었다. 효소 생산 유도 물질, Tween 80을 0.005% 수준으로 첨가하였을 때 가장 높은 효소 생산성을 보였으며 veratryl alcohol도 0.4 mM 수준에서 생산성 향상을 나타내었다.

• KSBB Journal
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• 제15권3호
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• pp.262-267
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• 2000

The white rot fungi Phanerochaete chrysosporium(IFO 31249) Trametes sp and Pleurotus sp. were studied for their ability to degrade Polycyclic Aromatic Hydrocarbons(PAHs) using anthracene and pyrene as model compounds. The disapperarance anthracene and pyrene of from cultures of wild type strains. P chrysosporium Trametes sp. and Pleurotus sp was observed However the activities of ligninolytic enzymes were not detected in P chrysosporium cultures during degradation while ligninolytic enzymes were detected in both culture of Trametes sp. and Pleurotus sp. Therefore our results showed that PAHs was degraded under ligninolytic as well as nonligninolytic conditions. The results also indicate that lignin peroxidase(LiP) mananese peroxidase(MnP) and laccase are not essential for the biodegradation of PAHs by white rot fungi.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2003년도 춘계학술발표논문집
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• pp.180-187
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• 2003

The lignin-degrading fungus Trichophyton sp. LKY-7 was immobilized in ca-alginate beads for laccase production and PCP remediation. The immobilized Trichopphyton sp. LKY-7 enabled the repeated use of this fungus for laccase production and produced high amount of laccase throughout 5 cycles incubation. As a laccase inducer oak wood meal(Quercus variabilis) seemed to be effective laccase inducer for Trichophyton sp. LKY-7, and the optimum addition amount was 1%(W/W) in glucose-peptone medium. Biotransformation of pentachlorophenol by immobilized Trichophyton sp. LKY-7 reached an efficency of up to 90% without toxic inhibition. Immobilized Trichophyton sp. LKY-7 might thus be applicable for semicontinuous laccase production and bioremediation to serve inoculum for reactor system.

• 생명과학회지
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• 제17권4호
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• pp.568-574
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• 2007

리그닌 분해 세균인 Pseudomonas sp. LG2는 lignocellulose 기질을 분해하여 APPL 화합물을 생성하는 균주이다. 이 균주를 BSG(brewer's spent grain)가 함유된 배지에서 배양한 배양액에서 APPL 화합물을 확인하였다. 세포외 조효소들의 유도에 관한 여러 가지 탄소원의 영향을 조사한 결과 glucose 배지에서는 xylanase의 효소활성만 확인 되었고 xylose, arabinose에서 배양한 조효소에서는 FAE 및 xylanase의 효소활성이 없었다. Oat spelt xylan, HBSG I(hydrolyzed brewer's spent grain I), HBSG II(hydrolyzed brewer's spent grain II) 및 AFBSG(autoclaved fraction from brewer's spent grain)를 탄소원으로 배양한 조효소에서는 FAE 및 xylanase의 효소활성이 확인됐다. Pseudomonas sp. LG2를 oat spelt xylan, HBSG I, HBSG II 및 AFBSG를 탄소원으로 사용하여 14일 동안 배양하면서 배양기간에 따른 세포외 효소들의 FAE와 xylanase 활성을 조사하였다. Xylanase의 최고 활성은 xylan을 탄소원으로 6일간 배양 했을때 5.3 U/mg으로 가장 높았으며, FAE의 최고 활성은 AFBSG를 탄소원으로 배양했을 때 배양 8일째 15.4 mU/mg으로 가장 높았다. Oat spelt xylan, HBSG I, HBSG II 및 AFBSG를 탄소원으로 사용하여 배양한 배지에 분리된 ferulic acid가 확인되었다. 세포외 효소의 FAE 활성은 methyl ferulic acid, methyl caffeic acid, methyl p-coumaric acid에 대해 esterase의 활성을 보였으나, methyl sinapinic acid, methyl vanillic acid 및 methyl gallic acid에 대해서는 esterase의 활성이 없었다.