Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Ligninolytic Enzyme Activity Produced by Phellinus igniarius 26005

Phellinus sp.에 의한 리그닌 분해효소의 생산

  • 윤재돈 (영남대학교 자연자원대학) ;
  • 하효철 ((주)풀무원 식문화 연구원) ;
  • 이종숙 (영남대학교 자연자원대학) ;
  • 김정애 (영남대학교 약학대학) ;
  • 이재성 (영남대학교 자연자원대학)
  • Published : 2004.09.30
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Abstract

The optimum conditions for lignin peroxidase production were studied. Lignin peroxidase was produced almost exclusively in stationary culture with the optimum media composition of malt extract 1 g, yeast extract 0.4 g, glucose 0.4 g and distilled water 100 ml. Tween 80 at 0.005% concentration and veratryl alcohol at 0.4 mM were very effective inducers for lignin peroxidase production.

Phellinus igniarius 26005에 의한 lignin peroxidase의 생산 조건의 최적화를 검토하였다. Lignin peroxidase는 진탕배양보다는 정치배양에서 생산성이 높았으며 최적 생산배지는 malt extract 1 g, yeast extract 0.4 g, glucose 0.4 g, 증류수 100 ml이었다. 효소 생산 유도 물질, Tween 80을 0.005% 수준으로 첨가하였을 때 가장 높은 효소 생산성을 보였으며 veratryl alcohol도 0.4 mM 수준에서 생산성 향상을 나타내었다.

Keywords

References

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