• Title/Summary/Keyword: SP data

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A Thermostable Protease Produced from Bacillus sp. DF 218 (Bacillus sp. DF218이 생산하는 내열성 단백질 분해효소)

  • Lee, Joung-Hee;Bai, Dong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.36 no.1
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    • pp.105-110
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    • 2004
  • Microorganism (strain DF 218) producing thermostable pretense was isolated from Korean soil and compost. It was Gram-positive, rod-shaped, aerobic, and spore-forming with yellowish white colony color, Temperature range for growth at pH 6.5 was $30-65^{\circ}C$, with optimum growth at $60^{\circ}C$. pH range for growth at $60^{\circ}C$ was 5-7 with optimum of 6.5, which indicates strain DF 218 to be thermophilic. The 16S rDNA sequence of strain DF 218 had 95% sequence similarity with that of Bacillus flexus. Based on physiological properties and phylogenetic analysis, we proposed the isolated strain as Bacillus sp. DF 218. Pretense was produced aerobically at $60^{\circ}C$ for 32 hr in a medium (pH 6.5) containing 1% each trypton, glucose, and NaCl. Its molecular weight was estimated as 61 kDa, with optimum temperature and pH of $60^{\circ}C$ and 7.5, respectively.

A Convergence Study on the Factors Influencing Nursing Students' Attitudes toward Standard Precautions: Focusing on the Health Belief Model (간호대학생의 표준주의 태도에 미치는 영향 요인에 관한 융합적 연구 : 건강신념모델 기반)

  • Kim, Mi-Ja;Yun, Seon-Jin
    • Journal of the Korea Convergence Society
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    • v.9 no.6
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    • pp.77-88
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    • 2018
  • "Standard Precautions" (SP) is important for nursing students as it will lead to the protection of health professionals from infectious diseases and prevention of the wide spread of epidemic illnesses. Therefore, this study investigated factors influencing nursing students' attitudes toward SP. Data were collected from 291 nursing students from May to June, in 2016. According to the results, perceived benefit (4.26) was the highest among sub factors of the health belief model, test score of SP was 78.8%, and attitude score (4 point scale) was 3.63. Perceived sensitivity, perceived benefits, perceived barriers, self-efficacy, knowledge about SP showed significant correlations with the attitudes towards SP. Among sub-factors of the health beliefs, perceived sensitivity (${\beta}=.152$, p<.05) and perceived barrier (${\beta}=-.125$, p<.05) were found to influence nursing students' attitudes toward SP (F= 5.680, p<.001). Hence, it can be concluded that a convergence education program promoting health belief is needed for improving attitudes toward SP among nursing students.

Design of an $SpO_2$ Transmission Agent based on ISO/IEEE 11073 Standard Protocol (ISO/IEEE 11073 표준 프로토콜 기반의 산소포화도 전송 에이전트 설계)

  • Pak, Ju-Geon;Im, Sung-Hyun;Park, Kee-Hyun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.462-465
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    • 2011
  • A pulse oximeter is a device which provides non-invasive estimate of percentage oxygen saturation of haemoglobin (SpO2). Due to the limitations of resources of personal health devices (PHDs) including pulse oximeters, they generally transmit the estimated data to a remote monitoring server through a close manager (e.g. mobile device or PC). Therefore, communication protocols between PHDs and a manager is an important research topic in terms of interoperability. In this paper, we present design results of an SpO2 transmission agent based on the ISO/IEEE 11073 (X73) protocol. The protocol is an international standard for PHDs. The agent is an embedded program which generates X73 messages from the estimated pulse rates and SpO2, and transmits the messages to a close manager. The agent consists of a Session, Message and Memory Handler. The Session Handler manages a communication session with the manager, and the Message Handler generates and analyzes the exchanged messages according to the X73 protocol. The Memory Handler extracts pulse rates and SpO2s which are stored in a memory of the pulse oximeter. The SpO2 transmission agent allows pulse oximeters to communicate with managers based on x73 standard. Consequently, the interoperability between the pulse oximeters and the managers is guaranteed.

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Thermal-hydro-mechanical Modelling for an Äspö prototype repository: analysis of thermal behavior (Äspö 원형 처분장에 대한 열-수리-역학적 모델링 연구: 열적 거동 해석)

  • Lee, Jae Owan;Birch, Kenneth;Choi, Heui-Joo
    • Tunnel and Underground Space
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    • v.23 no.5
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    • pp.372-382
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    • 2013
  • Thermal-hydro-mechanical (THM) modeling is a critical R&D issue in the performance and safety assessment of a high-level waste repository. With an $\ddot{A}$sp$\ddot{o}$ prototype repository, its thermal behavior was analyzed and then compared with in-situ experimental data for its validation. A model simulation was used to calculate the temperature distributions in the deposition holes, deposition tunnel, and surrounding host rock. A comparison of the simulation results with the experimental data was made for deposition hole DH-6, which showed that there was a temperature difference of $2{\sim}5^{\circ}C$ depending on the location of the measuring points, but there was a similar trend in the evolution curves of temperature as a function of time. It was expected that the coupled modeling of the thermal behavior with the hydro-mechanical behavior in the buffer and backfill of the $\ddot{A}$sp$\ddot{o}$ prototype repository would give a better agreement between the experimental and model calculation results.

Occurrence of Grapholita dimorpha in Korean Pear Orchards and Cross-trapping of Its Sibling Species, Grapholita molesta, to a Pheromone Lure (국내 배과원에 복숭아순나방붙이의 발생과 유사종 복숭아순나방의 페로몬 트랩 교차 유인)

  • Jung, Chung Ryul;Ahn, Jeong Joon;Eom, Hoon Sik;Seo, Jung Heun;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.479-484
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    • 2012
  • The occurrence of plum fruit moth, Grapholita dimorpha, has been recently reported in apple orchards in Korea. It has been suspected that G. dimorpha and its related species, G. molesta, may occur simultaneously in other host plants. This study reports the occurrence of G. dimorpha in pear orchards of different localities in Korea. The identification of G. dimorpha was determined by morphological characters and the DNA marker. The cross-trapping of both species may be possible because the major sex pheromone (SP) compositions for the two species are similar. From the monitoring data, G. dimorpha and G. molesta were caught in SP lure traps of G. dimorpha and both species were also caught in SP lure traps of G. molesta. This cross-trapping of G. molesta to a SP lure of G. dimorpha varied significantly among pear orchards in different geographical localities. Furthermore, the occurrence peaks of the two species were not coincidental in all monitored orchards. These suggest that monitoring data obtained from each SP trap of both species in pear orchards may be mixed with two species, which would result in the over-estimation of population density and peak frequency on both species in pear orchards.

High-Speed Implementation and Efficient Memory Usage of Min-Entropy Estimation Algorithms in NIST SP 800-90B (NIST SP 800-90B의 최소 엔트로피 추정 알고리즘에 대한 고속 구현 및 효율적인 메모리 사용 기법)

  • Kim, Wontae;Yeom, Yongjin;Kang, Ju-Sung
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.28 no.1
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    • pp.25-39
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    • 2018
  • NIST(National Institute of Standards and Technology) has recently published SP 800-90B second draft which is the document for evaluating security of entropy source, a key element of a cryptographic random number generator(RNG), and provided a tool implemented on Python code. In SP 800-90B, the security evaluation of the entropy sources is a process of estimating min-entropy by several estimators. The process of estimating min-entropy is divided into IID track and non-IID track. In IID track, the entropy sources are estimated only from MCV estimator. In non-IID Track, the entropy sources are estimated from 10 estimators including MCV estimator. The running time of the NIST's tool in non-IID track is approximately 20 minutes and the memory usage is over 5.5 GB. For evaluation agencies that have to perform repeatedly evaluations on various samples, and developers or researchers who have to perform experiments in various environments, it may be inconvenient to estimate entropy using the tool and depending on the environment, it may be impossible to execute. In this paper, we propose high-speed implementations and an efficient memory usage technique for min-entropy estimation algorithm of SP 800-90B. Our major achievements are the three improved speed and efficient memory usage reduction methods which are the method applying advantages of C++ code for improving speed of MultiMCW estimator, the method effectively reducing the memory and improving speed of MultiMMC by rebuilding the data storage structure, and the method improving the speed of LZ78Y by rebuilding the data structure. The tool applied our proposed methods is 14 times faster and saves 13 times more memory usage than NIST's tool.

A new Korean red algal species, Haraldiophyllum udoensis sp. nov. (Delesseriaceae, Rhodophyta)

  • Kim, Myung-Sook;Kang, Jeong-Chan
    • ALGAE
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    • v.26 no.3
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    • pp.211-219
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    • 2011
  • The genus Haraldiophyllum comprises seven species worldwide. Six of these are endemics with limited distributions, whereas the type species H. bonnemaisonii has been reported from the Atlantic Ocean. In Korea, H. bonnemaisonii has been previously recorded from the southern coast. During a red algal collection at Udo, Jeju Island, Korea, we found a potentially undescribed Haraldiophyllum species and analyzed its morphology and rbcL sequences. Herein we describe a new species, H. udoensis sp. nov., and compare our Udo specimen to similar congeners. This new species is characterized by one or several elliptical blades on a short cylindrical stipe with fibrous roots, blades that are monostromatic except at the base and on reproductive structures, a lack of network and microscopic veins, entire margins, lack of proliferations, growth through many marginal initials, and two distinct tetrasporangia layers. A phylogenetic rbcL sequence analysis demonstrated H. udoensis was distinct from the United Kingdom's H. bonnemaisonii, as well as from other species. Morphological and sequence data indicated a previous misidentification of H. udoensis as the type species H. bonnemaisonii. Based on maximum likelihood analysis, Myriogramme formed a sister clade with H. udoensis, with relatively low bootstrap support.

Morphology and phylogenetic relationships of Micractinium (Chlorellaceae, Trebouxiophyceae) taxa, including three new species from Antarctica

  • Chae, Hyunsik;Lim, Sooyeon;Kim, Han Soon;Choi, Han-Gu;Kim, Ji Hee
    • ALGAE
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    • v.34 no.4
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    • pp.267-275
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    • 2019
  • Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

Neosiphonia ramirezii sp. nov. (Rhodomelaceae, Rhodophyta) from Peru

  • Bustamante, Danilo Edson;Won, Boo Yeon;Cho, Tae Oh
    • ALGAE
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    • v.28 no.1
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    • pp.73-82
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    • 2013
  • The genus Neosiphonia Kim and Lee 1999 contains approximately 30 species worldwide. Unidentified samples from Peru are here described as a new species, Neosiphonia ramirezii sp. nov., on the basis of morphological and molecular data. N. ramirezii sp. nov. is characterized by the presence of a limited prostrate system, well-developed erect filaments, rhizoids cut off from pericentral cells by cross walls, four pericentral cells that are completely ecorticate, scarce trichoblasts, inconspicuous scar cells, procarps with three-celled carpogonial branches, spermatangial branches developed from basal cells of forked trichoblasts, and spirally arranged tetrasporangia. Our new species is distinct from N. flaccidissima (Hollenberg) Kim et Lee, N. sphaerocarpa (Borgesen) Kim et Lee, and N. savatieri (Hariot) Kim et Lee from the Pacific temperate coast of South America and from 14 Neosiphonia species reported worldwide by having limited prostrate filaments attached by numerous rhizoids, dichotomous ("Y" shaped) branches in the main axes, and scarce trichoblasts. Phylogenetic rbcL analyses confirm the placement of the new taxon as a distinct species in the genus Neosiphonia.

Vegetative Compatibility Groups and Pathogenicity Variation among Isolates of Fusarium oxysporum f.sp. melonis

  • Ahn, Il-Pyung;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.227-230
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    • 2000
  • A total of 90 isolates of Fusarium oxysporum f.sp. melonis, the causal agent of oriental melon (Cucumis melo var. makuwa) wilt, was isolated from symptomatic tissues of oriental melon from 4 provinces in Korea. These isolates were grouped into vegetative compatibility groups (VCGs) by demonstrating heterokaryosis through complementation using nitrate nonutilizing (nit) mutants. No self-incompatibility was observed in any of isolates. All isolates were grouped into 3 VCGs ; A, B, and C. iSOLATES BELONGING TO VCG A and VCG B accounted for 87% and 91% of the fungal population collected in 1991 and 1993, respectively. As the increment of cultivation period in the same field, the proportion of isolates belonging to VCG B increased whereas that of isolates belonging to VCG A decreased. Mean virulence of a total population increased as the increment of cultivation period in the same field. Isolates belonging to VCG B showed the highest increment of virulence. These data suggest that replanting of a host plant in the same field may cause increase of virulence in the pathogens. Furthermore, virulence of F. oxysporum f.sp. melonis isolates is related to the VCGs.

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