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Morphological Variation of Enterobacter sp. BL-2 in Acetate-mediated pH Environment for Excretive Production of Cationic Microbial Polyglucosamine Biopolymer

  • Son, Mi-Kyung;Hong, Soo-Jung;SaGong, Kuk-Hwa;Lee, Yong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.104-106
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    • 2008
  • Enterobacter sp. BL-2 excretively produced a unique cationic polyglucosamine biopolymer PGB-1 comprised of more than 95% D-glucosamine in an acetate-mediated culture condition. The excretion of the biopolymer PGB-1 was closely associated with the cellular morphology of Enterobacter sp. BL-2, a feature highly dependable on the pH of the medium. The initially formed uneven and irregular surface cells were aggregated into the cell-biopolymer network structure connected by the adhesion modules of the cell-bound biopolymer. The excretive production of the biopolymer PGB-1 coincided with the disruption of the cell-biopolymer network, most actively at the medium pH of 8.0.

Inhibitory Effects of Cysteine on Growth and Protease Production of Preudomonas sp. RP-222 and its Mutant MR-3966 (Pseudomonas sp. RP-222와 변이주 MR-3966 의 생육 및 Protease 생산에 Cysteine이 미치는 저해효과)

  • Lee, Kwang-Soo;Kang, Shin-Kwon;Son, Bong-Soo;Roh, Jong-Soo;Kim, Gyeong-Sook;Chun, Sung-Sik;Sung, Nack-Kie
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.621-626
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    • 1994
  • Cysteine showed strong inhibitory effect on growth and protease production of Pseudo- monas sp. RP-222 and its mutant, MR-3966. Mid- to late-log phase cells were most sensitive to the presence of 10 mM cysteine. The inhibition caused by cysteine was almost completely overcome by addition of isoleucine, leucine and valine mixture to the medium, and inclusion of iso#leucine alone could greatly reduce the inhibitory effects of cysteine. Homocysteine and #cysteine, sulfur compounds having similar structure as cysteine, inhibited to varying degrees the growth of both strains. Cysteine and homocysteine were strong inhibitors of threonine deaminase but not transa#- minase B. These results suggest a relationship between the growth-inhibitory effects of cysteine and other sulfur compounds and the inhibition of isoleucine synthesis at the level of threonine deaminase.

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Stabilization for Multirate Sampled-data Control Systems in case of Open-loop Unstable Plant

  • Son, Seok-Bo;Kim, Young-Baek;Park, Chansik;Lee, Sang-Jeong
    • 제어로봇시스템학회:학술대회논문집
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    • 2000.10a
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    • pp.488-488
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    • 2000
  • This paper proposes a stabilizing controller for multirate sampled-data systems which have a periodic output The proposed controller has IMC structure, and can be decomposed into a ye-stabilizing controller, an output estimator, a filtered disturbance estimator and the inverse of the fast ye-stabilized plant model. We assume that the plant is open-loop unstable and the disturbance consists of a sum of finite number of sinusoids with different frequencies. A sufficient condition for maintaining observability in the multirate sampled-data system is derived and a design strategy for filtered disturbance rejection is proposed. In addition, we propose a design method (or the plant output estimator. The simulation results show that the proposed stabilizing controllers can stabilize the plant

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Effect of Process Parameters on Forming Characteristics of Selective Laser Sintered Fe-Ni-Cr Powder (Fe-Ni-Cr 분말의 선택적 레이저 소결 적층시 공정변수에 따른 조형특성)

  • Joo, B.D.;Jang, J.H.;Yim, H.S.;Son, Y.M.;Moon, Y.H.
    • Transactions of Materials Processing
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    • v.18 no.3
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    • pp.262-267
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    • 2009
  • Selective laser sintering is a kind of rapid prototyping process whereby a three-dimensional part is built layer wise by laser scanning the powder. This process is highly influenced by powder and laser parameters such as laser power, scan rate, fill spacing and layer thickness. Therefore a study on fabricating Fe-Ni-Cr powder by selective laser sintering has been performed. In this study, fabrication was performed by experimental facilities consisting of a 200W fiber laser which can be focused to 0.08mm and atmospheric chamber which can control atmospheric pressure with argon. With power increase or energy density decrease, line width was decreased and line surface quality was improved with energy density increase. Surface quality of quadrangle structure was improved with fill spacing optimization.

Weight Functions for Notched Structures with Anti-plane Deformation

  • An, Deuk-Man;Son, In-Ho
    • International Journal of Precision Engineering and Manufacturing
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    • v.8 no.3
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    • pp.60-63
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    • 2007
  • Weight functions in fracture mechanics represent the stress intensity factors as weighted averages of the externally impressed boundary tractions and body forces. We extended the weight function theory for cracked linear elastic materials to calculate the notch stress intensity factor of a notched structure with anti-plane deformation. The well-known method of deriving weight functions by differentiation cannot be used for notched structures. By combining an appropriate singular field with a regular field, we derived weight functions for the notch stress intensity factor. Closed expressions of weight functions for notched cylindrical bodies are given as examples.

Chemical Structure of Bioactive lsoflavonoids from the Flowers of Pueraria lobata (Flos Puerariae) (길화의 생물활성 이소플라보노이드류의 화학구조)

  • Cho, Yong-Jin;Jeong, Dong-Youn;Choi, Hong-Dae;Park, Jong-Hee;Son, Byeng-Wha
    • Korean Journal of Pharmacognosy
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    • v.29 no.3
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    • pp.193-197
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    • 1998
  • Two isoflavonoids were isolated from the flowers of Pueraria lobata (Flos puerariae) guided initially fractionation based on brine shrimp lethality assay. The structures were identified as 4',5,7-trihydroxy-6-methoxyisoflavone (tectorigenin) and 4',7-dihydroxy-6-methoxyisoflavone (glycitein), respectively, on the basis of their spectroscopic and physicochemical evidences.

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Actinofuranone C, a New 3-Furanone-Bearing Polyketide from a Dung Beetle-Associated Bacterium

  • Um, Soohyun;Bang, Hea-Son;Shin, Jongheon;Oh, Dong-Chan
    • Natural Product Sciences
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    • v.19 no.1
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    • pp.71-75
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    • 2013
  • Actinofuranone C (1), a new 3-furanone-bearing polyketide, was isolated from an actinobacterium (Amycolatopsis sp.) associated with a female of the dung beetle, Copris tripartitus Waterhouse. The structure of actinofuranone C was elucidated by the spectroscopic interpretation of NMR, mass, UV, and IR data. The discovery of actinofuranone C indicates that chemical investigation of insect-associated microorganisms would be an effective strategy to explore natural chemical diversity.

Development of physically based 3D computer simulation code TRICSI for ion implantation into crystalline silicon

  • Son, Myung-Sik;Lee, Jun-Ha;Hwang, Ho-Jung
    • Journal of Korean Vacuum Science & Technology
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    • v.1 no.1
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    • pp.1-12
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    • 1997
  • A new three-dimensional (3D) Monte Carlo ion implantation simulator, TRICSI, has been developed to investigate 3D mask effects in the typical mask structure for ion implantation into crystalline silicon. We present the mask corner and mask size effects of implanted boron range profiles, and also show the calculated damage distributions by applying the modified Kinchin-Pease equation in the single-crystal silicon target. The simulator calculates accurately and efficiently the implanted-boron range profiles under the relatively large implanted area, using a newly developed search algorithm for the collision partner in the single-crystal silicon. All of the typical implant parameters such as dose, tilt and rotation angles, in addition to energy can be used for the 3D simulation of ion implantation.

Characteristics of Pinitol as a Functional Food Biomaterial (건강기능성 식품소재로서 pinitol의 특성)

  • Son, Min-Sik;Seo, Myung-Seon;Lee, Sang-Han
    • Journal of Life Science
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    • v.19 no.1
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    • pp.147-151
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    • 2009
  • D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.