• 지질공학
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• 제19권4호
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• pp.493-499
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• 2009

우리나라는 암석자원이 풍부하기 때문에 콘크리트표면 차수벽형 석괴댐(CFRD)의 시공이 증가하고 있는 실정이다. 콘크리트표면 차수벽형 석괴댐에서의 표층부 기초지반인 Bedding Zone 구간은 콘크리트 차수벽을 균등하게 지지할 수 있는 지지력과 콘크리트 차수벽의 누수시 세립토의 유실을 억제할 수 있는 차수성을 유지하여야 한다. 따라서 Bedding Zone 구간의 차수성 확보 여부가 댐의 안정성을 좌우하는 주요 요인이 될 수 있다. 본 연구에서는 기존의 시방서에 제시된 Zone-1 구간의 설계입도분포를 만족하는 시험재료를 선정한 후, 차수성을 평가하기 위하여 실내시험을 실시하였다. 시험 분석 결과 투수계수, 건조단위중량 및 흙의 입도분포에 따른 차수효과의 상관관계를 제시하였다. 특히, 투수계수와 건조단위중량의 상관관계, No. 4체 중량통과백분율과 투수계수 관계가 제시되었고, 또한 시간경과에 따른 투수계수의 변화도 제시되었다.

• Earthquakes and Structures
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• 제12권3호
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• pp.321-332
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• 2017

The reverse fault is a dangerous geological hazard faced by buried steel pipelines. Permanent ground deformation along the fault trace will induce large compressive strain leading to buckling failure of the pipe. A hybrid pipe-shell element based numerical model programed by INP code supported by ABAQUS solver was proposed in this study to explore the strain performance of buried X80 steel pipeline under reverse fault displacement. Accuracy of the numerical model was validated by previous full scale experimental results. Based on this model, parametric analysis was conducted to study the effects of four main kinds of parameters, e.g., pipe parameters, fault parameters, load parameter and soil property parameters, on the strain demand. Based on 2340 peak strain results of various combinations of design parameters, a semi-empirical model for strain demand prediction of X80 pipeline at reverse fault crossings was proposed. In general, reverse faults encountered by pipelines are involved in 3D oblique reverse faults, which can be considered as a combination of reverse fault and strike-slip fault. So a compressive strain demand estimation procedure for X80 pipeline crossing oblique-reverse faults was proposed by combining the presented semi-empirical model and the previous one for compression strike-slip fault (Liu 2016). Accuracy and efficiency of this proposed method was validated by fifteen design cases faced by the Second West to East Gas pipeline. The proposed method can be directly applied to the strain based design of X80 steel pipeline crossing oblique-reverse faults, with much higher efficiency than common numerical models.

• 지적과 국토정보
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• 제45권2호
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• pp.161-173
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• 2015

최근 기후변화에 따른 집중호우로 산사태 및 토석류가 발생하여 많은 인명 및 재산피해가 발생하고 있다. 본 연구에서는 UAV 측량기술을 활용하여 산사태 평가에 필요한 DSM과 정사영상을 신속하게 구축하였으며, 이를 무한사면해석모형에 적용하여 산사태 위험도를 평가하였다. 대상지역에 대한 산사태 위험도를 평가한 결과, 산사태 위험도 구간인 $SI{\leq}1.0$에서의 분포면적이 $46,396m^2$로 분석되었으며 분포비율로는 전체지역의 18.2%로 나타났다. 특히 산사태 발생이 매우 심각하여 사면보강 대책을 시급히 시행해야 되는 구간인 $SI{\leq}0.0$의 면적은 $7,988m^2$로서 전체지역의 0.8%를 차지하는 것으로 분석되었다. 또한 사면안정지수에 의한 산사태 위험도와 물골분석에 의한 토석류 위험도를 종합적으로 검토함으로써, 집중호우 시 산사태에 따른 토석류 위험지역을 선정할 수 있었다. 본 연구에서 분석한 산사태 및 토석류 위험지역은 향후 사면보강이나 주민안전대책 수립을 위한 의사결정 자료로 활용될 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제4권2호
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• pp.113-118
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• 1994

A bacterial strain NS-83 isolated from soil was able to produce an extracellular thermostable protease. The strain was identified as Pseudomonas aeruginosa based on its morphological and physiological characteristics. A thermostable protease from this strain has been purified to homogeneity as judged by SDS-PAGE and isoelectric focusing. The purification procedures included hydrophobic interaction, ion exchange, and gel filtration chromatography. The $M_r$ and the pl of the enzyme were 32,000 and 5.9, respectively. The optimal pH at 55$^{\circ}C$ and the optimal temperature at pH 7.0 were 8.0 and 60$^{\circ}C$, respectively. The D-values of the enzyme at 60, 65, and 70$^{\circ}C$ were 22, 2.1, and 0.75 hrs, respectively. The enzyme activity was significantly inhibited in the presence of 1 mM o-phenanthroline or EDTA, suggesting that the enzyme is metalloprotease. The $K_m$, and $V_{max}$ for Hammarsten casein were found to be 3.2 mg/ml and 0.918 unit/ml, respectively. These enzymatic properties were similar to those of elastase produced from P. aeruginosa IFO 3455, but the enzyme was clearly different from the reported elastase, in respect to $Ca^{++}$ effects on enzyme-thermostability. This property, together with amino acid composition analysis, confirmed that the enzyme differs from the known P. aeruginosa elastase.

• 한국응용곤충학회지
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• 제22권2호
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• pp.147-159
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• 1983

In general, herbicides have been classified according to selectivity, mobility. time of application, methods of application, mode of action and chemical property and structure. However, there was no generally accepted classification system for practical use in the field. The primary processes affected by the majority of herbicides are the growth process through cell elongation and/or cell division, the photosynthetic process specifically the light reaction, the oxidative phosphorylation and the integrity of the membrane systems. The usual approach in the study of the mechanism by which herbicides kill or inhibit the growth of plants is to initially determine the morphological phototoxicity systems, The mechanism by which a herbicide kills a plant or suppresses its development is actually the resultant effect of primary and secondary(or side) effects. In most instances, the death of the plant is due to the secondary effects. To induce the desired response, a herbicide must be able to gain entry into the plants and once inside, to be transported within the plant to its site(s) of activity in concentrations great enough. Obstacles to the entry and movement of herbicides in plants are generally classified by leaf and soil obstacles, translocation obstacles and biochemical obstacles, and these obstacles are also strongly influenced by plant species and by environmental factors such as light, temperature, rainfall and relative humidity. And hence, in most instances, results obtained from laboratory or greenhous vary from those of field experiment. Author attempted to classify herbicides from the field experiment using the two-dimensional ordination analysis to obtain practical information for selecting effective herbicides or to choose effective herbicide combinations for increasing herbicidal efficacy or reducing the chemical cost. Based on this two-dimensional diagram, desired herbicides or combinations were selected and further investigated for the interaction effects whether these combinations are synergistic, additive or antagonistic. From the results, it was concluded that these new approach could possibly be give more comprehensive informations about effective use of herbicide than any other systems.

• 한국지반환경공학회 논문집
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• 제10권4호
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• pp.33-40
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• 2009

성토 시공시, 경제적인 시공을 위해 절 성토량의 균형은 중요하게 고려되어야 할 사항이다. 그러나 실제 완성기면까지 성토시 성토체 자체의 압축침하와 연약지반 성토시 발생하는 제체하부 원지반의 압밀침하, 암 버럭 사이의 간극 메움에 의한 침하와 강우와 유출시 발생하는 토량 손실에 의한 성토량의 부족분이 발생하고 있다. 성토량의 부족분에 대한 지침은 도로공사 시방서에 토량환산계수를 통해 설계하도록 되어 있지만 성토체 자체의 압축침하에 의한 부족분은 고려되고 있지 않다. 따라서 본 연구에서는 이세전(伊勢田)(1972)와 석정(石井)(1976)가 제안하고 있는 성토체의 제체압축침하량 산정기법을 적용하여 국내 여러 현장의 성토체를 대상으로 발생가능한 압축침하량을 산정하였다. 본 연구결과 성토고 및 성토재의 종류에 따라 성토고의 약 3~10%내외의 전침하량이 발생하는 것으로 나타났다. 그러므로 실제 설계시 성토재의 특성 및 성토고를 고려한 성토체의 즉시침하 및 크리프 침하를 반영한 설계가 필요할 것으로 판단된다.

• 미생물학회지
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• 제47권3호
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• pp.225-230
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• 2011

유일 탄소원으로 palm kernel meal (PKM)과 밀기울을 함유한 최소배지에서 농후배양하여 작물 재배 토양으로부터 xylan과 locust bean gum (LBG)에 대한 분해활성이 있는 균을 분리하였다. 분리균 YB-1106의 16S rRNA 유전자 염기서열을 조사한 결과 Microbacterium arabinogalactanolyticum와 98% 유사도를 보였다. 분리균의 xylanase는 밀기울, oat spelt xylan, 미강 및 xylose와 같은 부가탄소원에 의해 생산성이 증가된 반면에 mannanase는 LBG와 PKM에 의해 생산성이 증가되었다. 특히 Xylanase는 밀기울 2%를 첨가한 배지, mannanase는 1% LBG를 첨가한 배지에서 각각 생산성이 가장 높았으며 모두 정지기에서 생산이 되었다. 분리균의 배양 상등액은 $50^{\circ}C$와 pH 6.0에서 mannanase의 최대활성을 보였으며, $50^{\circ}C$와 pH 6.5에서 xylanase의 최적반응 활성을 나타냈다. Mannanase에 의해 분해된 LBG와 xylanase에 의해 분해된 xylan으로부터 각각 분해산물로 올리고당이 관찰되었다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1142-1149
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• 2004

Streptococcus mutans has the capacity of inducing dental caries. Thus, to develop a novel way of preventing dental caries, a cell wall hydrolase-producing strain was isolated and its characteristics were investigated. Among 200 alkalophilic strains isolated from soil, 8 strains exhibited lytic activities against Streptococcus mutans. However, strain YU5215 with the highest cell wall hydrolase activity was selected for further study. Strain YU5215 was identified as a novel strain of Bacillus based on analyzing its 16S rDNA sequence and Bergey's Manual of Systematic Bacteriology, and thus designated as Bacillus mutanolyticus YU5215. The optimal conditions for the production of the cell wall hydrolase from Bacillus mutanolyticus YU5215 consisted of glucose ($0.8\%$), yeast extract ($1.2\%$), polypeptone ($0.5\%$), $K_{2}HPO_{4}\;(0.1\%$), $MgSO_{4}{\cdot}7H_{2}O$ ($0.02\%$), and $Na_{2}CO_{3}\;(1.0\%$) at pH 10.0. Bacillus mutanolyticus YU5215 was cultured at 30^{circ}C for 72 h to produce the cell wall hydrolase, which was then purified by acetone precipitation and CM-agarose column chromatography. The molecular weight of the lytic enzyme was determined as 22,700 Da by SDS-PAGE. When the cell wall peptidoglycan of Streptococcus mutans was digested with the lytic enzyme, no increase in the reducing sugars was observed, while the free amino acids increased, indicating that the lytic enzyme had an endopeptidase-like property. The amino terminus of the cell wall peptidoglycan digested by the lytic enzyme was determined as a glutamic acid, while the lytic site of the lytic enzyme in the Streptococcus mutans peptidoglycan was identified as the peptide linkage of L-Ala and D-Glu.

• Journal of Biosystems Engineering
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• 제28권5호
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• pp.411-420
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• 2003

Physical and cultural properties were investigated on the rice-seed pellets made by the pelleting machine(Yu, 2003) as the changes with mixing ratios of soil to rice seed of 6 : 1, 7 : 1, and 8 : I, and rotating speeds of forming rolls of 7, 10, and 13 rpm. Average weight, average diameter, and average sphericity of the pellets were 1.70 g, 12.0 ㎜, and 99.1 ％, respectively. Average number of seeds per pellet was more than 3, and almost all pellets had more than 3 seeds in the cases of mixing ratios of 6 : 1, and 7 : 1 at the forming rolls' speed of 7 rpm. Gradual drying was needed because rapid drying caused cracks on surface of the pellets. Compression strength of the pellets dried in shady room was in the range of 132 ∼ 152 N, which was enough for handling. Comparing with the previous pellets(Park, 2002), average number of seeds per pellet, ratio of pellets including more than 3 seeds, and compression strength increased due to the effects of pressure feed of pellet materials, and improvements of the forming rolls. Emergence ratio of the pellets made at the mixing ratio of 6 : 1 and the forming rolls' speed of 7 rpm, was 100 % on dry paddy and was 97 % on flooded paddy surface. Good growth characteristics, and yield except number of seedling stand and ratio of missing plant were shown in planting of the pellets made at the mixing ratio of 7 : 1 and the forming rolls' speed of 7 rpm on flooded paddy surface field. Considering the cultural results, the mixing ratio of 6 : 1, and the forming rolls' speed of 7 rpm seems to be optimum operating condition for the improved pelleting machine.

• 한국측량학회지
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• 제34권3호
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• pp.309-317
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• 2016

토석류는 태풍이나 집중호우 등에 의해 산사태가 일어나 토석이 물과 함께 하류로 밀려 떠내려가는 현상을 의미한다. 특히 우리나라는 지형학적 특성상 전체 국토의 약 70%가 산지로 구성되어 있고 매년 6~8월 집중적으로 태풍이나 집중호우가 발생하여 이 시기에 발생한 산사태 및 대규모 토석류로 인한 인명피해는 전체 재난 사망자의 약 20%에 이르고 있다. 따라서 이러한 토석류의 발생 규모를 예측하기 위한 핵심적인 부분은 복잡한 3차원 지형에서의 토석류 거동, 침식 및 퇴적 메커니즘을 이해하여야 한다. 본 연구에서는 강원도 평창군의 실제 규모 토석류 실험 전후에 나타난 지형적인 변화 및 변화가 발생한 지역에서의 지형학적 특성 등의 정량적 변화 분석 등을 수행하였으며, 이를 위해 발생 전후의 정량적 변화를 관측할 수 있는 GSD 10cm LiDAR DEM 데이터를 조사에 활용하였다.