• Title/Summary/Keyword: SOD2

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Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress (산화적 스트레스상태에서 B16F10 Murine Melanoma 세포의 항산화효소 활성에 대한 흑축추출물의 효과)

  • 김안근;차은정
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.93-98
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    • 2004
  • The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.

Expression of Lily Chloroplastic Cu,Zn Superoxide Dismutase Enhances Resistance to Erwinia carotovora in Potatoes

  • Kim, Mi-Sun;Kim, Hyun-Soon;Kim, Yoon-Sik;Baek, Kwang-Hyun;Moon, Jae-Sun;Choi, Do-Il;Joung, Hyouk;Jeon, Jae-Heung
    • The Plant Pathology Journal
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    • v.23 no.4
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    • pp.300-307
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    • 2007
  • Previously, a chloroplast-localized Cu,Zn superoxide dismutase (chCu,ZnSOD) was isolated from lily and the sense- and antisense- sequences of the lily chCu,ZnSOD were used to transform potato plants. Two selected lines, the sense- and anti-sense strand of transgenic plants, were further characterized for resistance to Erwinia carotovora, which is a severe pathogen affecting potato plants. Only the sense-strand transgenic potato, which contained less $O_2^{.-}$ and more $H_2O_2$ than wild-type and antisense-strand transgenic plants, showed increased resistance to E. carotovora. Additional studies using $O_2^{.-}$ or $H_2O_2$ scavengers in wild-type, sense-strand, and antisense-strand transgenic plants suggest that resistance to E. carotovora is induced by reduced $O_2^{.-}$ and is not influenced by $H_2O_2$. To the best of our knowledge, this report is the first study suggesting that resistance to E. carotovora is enhanced by reduced $O_2^{.-}$, and not by increased amounts of $H_2O_2$.

The Effect of Heat Application on Ischemia-Reperfusion Injury to Quadriceps Femoris Muscle of the Rats (온열적용이 대퇴사두근의 허혈-재관류 후 SOD 발현에 미치는 영향)

  • Choi Jin-Ho;Kim Jin-Sang
    • The Journal of Korean Physical Therapy
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    • v.15 no.1
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    • pp.171-184
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    • 2003
  • The purpose of this study was to investigate the effect of heat application on ischemia-reperfusion injury to quadriceps femoris muscle of the rats. Nine weeks old male Sprague-Dawley white rats were divided into five groups: 1) control(only reperfusion following ischemia), 2) heat application before reperfusion following ischemia(PreHeat), 3) heat application after reperfusion following ischemia(PostHeat). All groups were 30 minute, 1 hour, 3 hours reperfusion after 2 hours ischemia with clamping abdominal artery, and investigate superoxide dismutase(SOD) immunohistochemical reactions for quadriceps femoris muscle of the rat. SOD immunohistochemical reaction of experimental groups were more than the control group.

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Antioxidant activity of ethanol extracts from the root and bark of Ulmus davidiana

  • Sim, Ki Hyeon;Han, Young Sil
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.133-140
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    • 2019
  • Antioxidant activities of Ulmus davidiana root and bark extracts were evaluated by various antioxidant tests, including DPPH radical-scavenging, nitric oxide-scavenging, superoxide anion radical-scavenging, and ABTS radical-scavenging assays, and superoxide dismutase (SOD)-like activity and reducing power analysis, along with the determination of total phenolic and flavonoid contents. Both the extracts showed strong antioxidant activities by these testing methods. Ulmus davidiana root extract possessed strong reducing power and nitric oxide-scavenging activity, and high scavenging activities against free radicals including the superoxide anion, and the ABTS and DPPH radicals, but a weaker scavenging activity of SOD. In contrast, the Ulmus davidiana bark extract exhibited a strong SOD-like activity, but all the other activities were weak. It was observed that the total phenolic and flavonoid contents of the Ulmus davidiana root extract were higher than those of the Ulmus davidiana bark extract.

Activities of Antioxidant Enzymes in Serum of Rats Feeding Soybean Extract (콩 추출물 투여가 흰쥐 혈청의 항산화효소 활성에 미치는 영향)

  • Yun Hong-Tae;Moon Jung-Kyung;Park Keum-Yong;Kim Yang-Ho;Shin Mi-Kyung;Kim Yeung-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.136-140
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    • 2005
  • This study was conducted to determine antioxidative enzyme activity in serum of rats as affected by soybean cultivar, extract feeding concentration and fat dietary. In all cultivars, activities of antioxidant enzymes in treatment of various fat compositions and cholesterol followed by feeding soy-extracts were higher than in nonfeeding control, however, no significant differences existed in statistically. The activities of SOD and GSHpx in serum of rats when fed with various fat dietary were higher in Geomjeongkong 1 than in Tawonkong and Hwangkeumkong. Enzyme activities of SOD and GSHpx with each fat compositions in treatment soy-extracts was no significant differences statistically. There was significant differences among cultivars and concentrations of soy-extracts in interaction effect. Also, the activities of GSHpx was significant differences among cultivars and concentrations of soy-extracts and fat composition in interaction effect.

Study on Antioxidant Aaction of Sibjeondaebo-tang (십전대보탕의 항산화작용에 관한 연구)

  • Heo Jun Young;Kim Byoung Soo;Kang Jung Soo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.2
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    • pp.356-362
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    • 2003
  • In order to examine the antioxidant activities of SJDBT(Sibjeondaebo-tang), the study was done through measurement of parameters such as LPO(lipidperoxidation), GSH(glutathione), SOD(superoxidation dismutase), catalase, GOT, GPT, ALP. The results were obtained as follows: For the weight changes, the kidney and testis of group given SJDBT showed decrease in the weight compared to the control group, and the left cerebrum, right cerebrum, cerebellum and liver of group given SJDBT showed increase. But the changes were not significant. The left cerebrum and right cerebrum of group given SJDBT showed significant decrease in the content of LPO compared to the control group, and in the activity of SOD and catalase showed significant increase. The cerebellum of group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of SOD and catalase showed significant increase. For the changes of LPO, GSH in the liver, the group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of catalase showed significant increase. For the changes of LPO in the kidney, the group given SJDBT showed significant decrease in the content of LPO compared to the control group. For the changes of LPO, GSH and the activity of SOD, catalase in the testis, the group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of SOD and catalase showed significant increase. From above results, the antioxidant action of SJDBT is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of SJDBT.

Mechanism Study of Flowable Oxide Process for Sur-100nm Shallow Trench Isolation

  • Kim, Dae-Kyoung;Jang, Hae-Gyu;Lee, Hun;In, Ki-Chul;Choi, Doo-Hwan;Chae, Hee-Yeop
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.68-68
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    • 2011
  • As feature size is smaller, new technology are needed in semiconductor factory such as gap-fill technology for sub 100nm, development of ALD equipment for Cu barrier/seed, oxide trench etcher technology for 25 nm and beyond, development of high throughput Cu CMP equipment for 30nm and development of poly etcher for 25 nm and so on. We are focus on gap-fill technology for sub-30nm. There are many problems, which are leaning, over-hang, void, micro-pore, delaminate, thickness limitation, squeeze-in, squeeze-out and thinning phenomenon in sub-30 nm gap fill. New gap-fill processes, which are viscous oxide-SOD (spin on dielectric), O3-TEOS, NF3 Based HDP and Flowable oxide have been attempting to overcome these problems. Some groups investigated SOD process. Because gap-fill performance of SOD is best and process parameter is simple. Nevertheless these advantages, SOD processes have some problems. First, material cost is high. Second, density of SOD is too low. Therefore annealing and curing process certainly necessary to get hard density film. On the other hand, film density by Flowable oxide process is higher than film density by SOD process. Therefore, we are focus on Flowable oxide. In this work, dielectric film were deposited by PECVD with TSA(Trisilylamine - N(SiH3)3) and NH3. To get flow-ability, the effect of plasma treatment was investigated as function of O2 plasma power. QMS (quadruple mass spectrometry) and FTIR was used to analysis mechanism. Gap-filling performance and flow ability was confirmed by various patterns.

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Assessment of Antioxidative Capacity in Relation to Seed Trails of Rice Varieties

  • Song, Hong-Keun;Ahn, Joung-Kuk;Kim, Kwang-Ho;Lee, Sun-Joo;Baek, Jin-Yeong;Chung, Ill-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.544-553
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    • 2006
  • In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r=0.29***), DPPH (r=0.80***) and TBA (r=0.76***). Between the three activities, SOD was not positively correlated with DPPH (r=0.15*), while TBA was significantly correlated with DPPH value (r=0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).

Effects of Cutting Methods and Soil Depth on Sod Formation of Mentha spp. (박하 속 식물의 뗏장형성에 미치는 삽목방법과 토심의 영향)

  • Lee Chung-Il;Nam Sang-Yong
    • Journal of Bio-Environment Control
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    • v.15 no.2
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    • pp.190-195
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    • 2006
  • The purpose of this study was to figure out the best condition in growth and sod quality of Mentha spp. according to cutting methods and soil depth. Applemint (M. suaveolens), Peppermint (M. piperita), and Spearmint (M. spicata) were used. Regardless of cultivar and soil depth condition, the growth rate in top cutting was higher than layering method. The difference between layering and layering without apical meristem was not significant. Optimal condition for growth was 5cm depth of soil. However, the shallower the depth of the soil, the better quality of sod. Among three Mentha species, M suavelens showed plant height and node number and M. piperita had shoot number were higher than other variety. The best condition of sod was top cutting and 1cm depth of soil regardless of cultivar.