• 동아시아식생활학회지
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• 제14권4호
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• pp.338-342
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• 2004

This study was performed to measure the antioxidant effects of red ginseng extracts which antioxidation had been promoted through enzyme hydrolysis. In order to observe their tumor-suppressing effects, an anti-cancer medicine and Saponin-SOD, which was a highly antioxidant beverage made from red ginseng saponin adding SOD-like rice (with embryo buds) extracts, were administered to nude mice with large intestine cancer induced. There was a significant increase in the content of phenolic compounds as the enzyme was added. The red ginseng extracts showed a high electron-donating ability with the passage of time. The electron-donating ability was particularly high in the enzyme-treated red ginseng extract, and also observed as high in Saponin-SOD. The lipid-peroxide generation was inhibited depending on the concentration of Saponin-SOD added; the addition of 0.625% Saponin-SOD served to decrease the inhibition level up to 65% compared with the case of no addition (100%). As a result, it could be assumed that Saponin-SOD would strongly inhibit the oxidation of ghost membrane. After the cancer was induced in nude mice through the injection of SNUC-4 cell, there was a significant inhibition in the growth of tumors in nude mice into which Saponin-SOD were injected; the growth of tumors was gradually decreasing with the passage of time after the cancer induction. In particular, when Saponin-SOD was administered together with an anti-cancer medicine, the synergic effect was observed. In conclusion, Saponin-SOD, when used with an anti-cancer medicine, is expected to reduce the amount of free radical and lipid peroxide, which are known to cause harmful effects occurring from the internal application of medicine.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.225-228
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• 2004

Superoxide dismutase (SOD) is an important enzyme which catalyzes superoxide radicals to hydrogen peroxide. A Cu, Zn sod-like gene was found in Bombyx mori nuclear polyhedrosis virus encoding 151 amino acids. To demonstrate its function, a recombinant virus named dsBmNPV with deleted sod gene was constructed. It was discovered that the sod gene was not essential for viral replication. Studies on growth of budded virus in BmN cells and superoxide dismutase and catalase activities in vivo after dsBmNPV infection showed that the titer of dsBmNPV decreased obviously comparing to wild type BmNPV, the sod gene was effective on genomic DNA replication of baculovirus, the peak of SOD activity of silkworm infected with wt-BmNPV appeared between 36 and 48 hrs post infection, and with dsBmNPV, it did not appear. And the changes of CAT activity after infection were similar to SOD activity.

• 대한한의학방제학회지
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• 제8권1호
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• pp.299-318
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• 2000

In order to study on SOD like activity and Electric donor ability of Hedyotis diffusa $W_{ILLD}$, testing antibacterial effect on E. coli O157 which causes infectious inflammatory disease; measunng inhibitory effect on tyrosinase which stimulates melanin formation; and measuring the influence of it to SOD like activity, DPPH and TBARS which are related to ageing and carcinogenesis. The results of these experiments, are as follows. 1. In the antibacterial experiment with the extract of Hedyotis diffusa $W_{ILLD}$ on E. coli O157, there was no change in proliferation from the beginning of culture to two hours after, but proliferation inhibiting effect on E. coli O157 was detected from three hours after the beginning of culture. 2. Tyrosinase inhibitory effect was measured as $0.39{\pm}0.026%$. Compared with the control group, the effect was very slight. 3. The SOD like activity of the extract of Hedyotis diffusa $W_{ILLD}$. was measured as 21.33${\pm}$ 4.264%. Compared with the extracts of several other herbs, it was much more significant. 4. The DPPH of the extract of Hedyotis diffusa $W_{ILLD}$. was $53.3{\pm}0.91%$ when 0.02g was used and $83.5{\pm}1.82% $ when 0.05g was used. The result when 0.05g was used was more significant than 0.02g was used. 5. The TBARS of the extract of Hedyotis diffusa $W_{ILLD}$. was measured as 0.724 ${\pm}$0.O04MDAppm. Compared with the extracts of several other herbs, the result was more significant. From these results, we found that Hedyotis diffusa $W_{ILLD}$. can be used in therapy of dysentery with bloody stool and fever which is caused by infection with E. coli O157 and that it can be also used effectively as age resister or anticarcinogen.

• 한국미생물·생명공학회지
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• 제40권3호
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• pp.208-214
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• 2012

본 연구는 왕겨를 이용한 항산화, 항노화에 효과적인 초액을 대량생산하여 기능성을 검증하고 왕겨초액 내의 기능성물질을 분석하여 사업화의 가능성을 타진하고자 하였다. 정제된 왕겨초액을 분획하여 기능성이 확인된 부분을 HPLC 기기를 이용하여 확인한 결과 caffeic acid 외에 7종의 polyphenol 유래물질에서 기능성을 확인하였다. 기능성 화장품소재 시험으로는 세포 생존율 측정(MTT assay), 주름개선 (collagenase inhibition assay, elastase inhibition assay), 미백(tyrosinase inhibition assay), 항산화 시험(DPPH free radical scavenging assay, SOD-like activity, xanthine oxidase inhibition assay)이 실시하였다. 피부주름형성은 콜라겐의 합성과 분해가 중요한 원인으로 작용한다고 알려져 있지만, 최근 많은 연구에서 엘라스틴의 재형성과 분해 또한 주름형성 기전에서 중요한 작용을 하는 것으로 보고되고 있다. 왕겨초액은 $100{\mu}L/mL$의 농도에서 collagenase 저해효과가 100%, elastase 저해효과가 55% 이상으로 주름개선 효과가 우수하였다. 왕겨초액은 $100{\mu}L/mL$의 농도에서 tyrosine에 대한 tyrosinase 저해효과가 80% 이상으로 미백효과가 우수하게 나타났다. DPPH radical 소거율, xanthine oxidase 저해와 SOD-like 활성을 이용하여 항산화 효과를 측정하였다. 왕겨초액 $100{\mu}L/mL$의 농도에서 DPPH radical 소거율이 80%, xanthine oxidase 저해효과가 80%, 그리고 SOD-like 활성이 100%를 나타내었다. 따라서 본 연구를 통하여 정제 왕겨초액을 이용한 산업화를 위한 가능성을 타진하여 효과가 좋음을 확인할 수 있었다. 이와 같은 결과로 왕겨초액을 이용한 기능성 화장품의 소재로 뿐만 아니라, 식품 및 의약외품의 품목허가를 통하여 고부가가치의 상품화소재로의 가능성이 있다고 사료됩니다.

• 한국식품저장유통학회지
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• 제13권3호
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• pp.413-419
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• 2006

뜰보리수 열매의 식품영양학적 평가의 일환으로 추출용매별, 추출농도별에 따른 전자공여능, linolenic acid system을 이용한 항산화활성, SOD유사활성, superoxide anion radical 소거능, 아질산염 소거능 등을 측정하였다. 전자공여능은 물 추출물에서는 낮은 농도에서 비교적 높은 활성을 나타내었고, 에탄올과 메탄올 추출물의 농도가 증가할수록 항산화 활성이 증가하였으며, 메탄올 추출물에서 가장 높은 항산화 효과를 보였다. Linoleic acid system에 의한 항산화 활성은 추출물의 농도가 증가할수록 항산화 효과 또한 높아졌고, 물 추출물 보다는 에탄올 추출물과 메탄올 추출물에서 효과가 더 좋았다. SOD 유사활성은 추출물의 농도가 높을수록 SOD 활성도 높게 나타났으며, 메탄올 추출물에서 추출물 농도 2.0 mg/mL에서 가장 높은 활성을 타나내었다. Superoxide anion radical에 대한 저해정도는 물 추출물의 경우 추출물의 첨가농도가 낮을 때 높은 저해효과를 나타내었고, 에탄올과 메탄올 추출물에서는 추출물 첨가농도가 증가할수록 저해효과가 높았다. 각 추출물과 pH의 변화에 따른 아질산염 소거작용에 대한 결과는 pH가 낮을수록 농도가 증가할수록 소거효과는 높게 나타났고, 물 추출물의 pH 1.2에서 77.35%로 아질산염 소거능이 가장 높은 효과를 나타내었다. 에탄올, 메탄올 추출물도 물 추출물에서와 같이 pH가 낮을수록 추출물의 첨가농도가 증가할수록 아질산염 소거작용이 높았다.

• 한국식품영양과학회지
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• 제35권3호
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• pp.301-306
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• 2006

호부추(Allium tuberosum)와 실부추(Allium senescens)를 열수, 50% 에탄올 및 100% 에탄올 등의 추출용매를 사용하여 건물 중량의 10배에 해당되는 부피(w/v)일 때, 추출물의 생리활성을 탐색하였다. 전자공여작용의 경우 호부추의 50% 에탄올추출물에서 41.68%의 높은 전자공여능을 나타내었으며, SOD 유사활성을 측정한 결과 호부추의 열수추출물이 36.33%로 다른 추출물에 비해 높은 활성을 보여주었다. Tyrosinase 저해활성의 경우 두 종류 모두 열수추출물에서 높은 저해활성을 보여주었으며, 호부추가 실부추보다 다소 높은 활성을 나타내었다. 특히 비교물질인 0.01% L-ascorbate와 유사한 활성을 보여주었다. 총 폴리페놀 함량의 경우 두 종류 모두 열수추출물보다 50%, 100% 에탄올추출물에서 높은 함량을 나타내었다. 아질산염 소거작용을 측정한 결과 pH 1.2일 때 호부추와 실부추 모두 소거능이 높게 나타났다. 이와 같은 결과는 부추의 생리활성을 밝혀 기능성 소재로써 이용도가 크게 증가할 것으로 판단된다.

• 동의생리병리학회지
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• 제22권3호
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• pp.672-677
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• 2008

In order to explore the anti-oxidization effect of oriental medicine and cereal medium(OCM) where Tricholoma matsutake mycelia were cultured, measurement of hot water extract and UMPM(extraction method using ultra sonic waves, micro waves, micro bubble) extract, the total polyphenol content of crude polysaccharide from each extract, SOD-like activity, electron donating ability(EDA), xanthine oxidase inhibitory activity, and tyrosinase inhibitory activity was conducted. The total polyphenol content of each extract was found to be 16.36% for hot water extract(HE) group and 15.73% for UMPM extract(UE) group and the amount of crude polysaccharide precipitated into ethanol of extracts was found to be 8.79% for UMPM ethanol extract(UEE) group and 6.48% for hot water ethanol extract(HEE) group. As a result of measurement of SOD-like activity by concentration of each extract, it was found to be 96.17% for UE group, 91.23% for HE group, 91.33% for UEE group, and 87.11% for HEE group at 20 mg/mL. In the case of EDA, it was found to be 47.55% for UE group, 44.93% for HE group, 25.38% for UEE group, and 18.36% for HEE group. And in the cases of the rates of xanthine oxidase inhibitory activity and tyrosinase inhibitory activity, as the concentration of each extract increased, the inhibition rate increased accordingly. As a result of comparison between hot water extract method and UMPM extract method using extracts obtained from oriental medicine compound medium where Tricholoma matsutake mycelia were cultured, all of the extracts were judged to have a high anti-oxidization effect. In particular, UMPM extracts were found to have higher polyphenol content, SOD-like activity, EDA, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity compared to hot water extract method. In this regard, extracts obtained from OCM where Tricholoma matsutake mycelia were cultured are considered to have high availability as functional material when and if they are prepared using UMPM extract method.

• 한국산학기술학회논문지
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• 제16권8호
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• pp.5343-5350
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• 2015

본 연구의 목적은 대나무 줄기표면에서 처음 발견 하였으나, 아직까지는 연구가 미흡한 신 균주 Bacillus amyloliquefaciens NBF11-1의 생리활성 효과를 알아보기 위하여 청국장 전통 발효 균주인 Bacillus subtilis NG24를 대조군으로 하여 항산화효과에 대한 비교분석을 실시하였다. 청국장 추출물의 항산화 실험 결과에서 Total Polyphenol 추출물 함량의 측정 결과는 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1 시료에서 유의하게 증가하였다(p=0.032). SOD 유사활성과 DPPH radical 소거능 및 NO radical 소거능에서 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1을 포함한 시료에서 농도가 증가함에 따라 유의하게 증가하였다(p<.05). 추가적으로, 각 항산화 실험의 $IC_{50}$은 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1 시료에서 SOD 유사활성(p=0.045), DPPH radical 소거능(p=0.041), NO radical 소거능(p=0.019)과 같이 유의한 차이로 감소하였다.

• 융합정보논문지
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• 제11권8호
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• pp.201-206
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• 2021

본 연구 목적은 염화제일철(FeCl2)의 세포독성과 석잠풀(SJ) 추출물의 항산화 효과 및 FeCl2의 독성을 산화적 손상 측면에서 조사하였다. 이를 위해, 세포 생존율을 비롯한 과산소음이온(SAR)-소거능과 슈퍼옥사이드(SOD)-유사 활성능을 분석하였다. 본 연구에서 FeCl2는 처리 농도에 비례하여 유의한 세포 생존율의 감소를 보였으며, 중간 독성으로 나타났다. 또한, caffeic acid는 FeCl2로 감소 된 세포 생존율에 대해 유의한 증가를 보였다. FeCl2의 독성에 대한 SJ 추출물의 처리는 세포 생존율의 유의한 증가와 함께 SAR-소거 능과 SOD-유사 활성 능과 같은 항산화 효과를 보였다. 결론적으로, SJ 추출물과 같은 천연소재는 항산화제와 같은 대체물질 개발에 있어 기초자료로서의 활용적 가치가 클 것으로 생각된다.

• 대한의생명과학회지
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• 제23권4호
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• pp.339-347
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• 2017

This study was performed to evaluate the cytotoxicity induced by ischemia-like condition (ILC) in cultured neuroglial cells (C6 glioma cells). The protective effect of kaempferol (KAE), flavonoid against the cytotoxicity induced by ILC induction was assessed. In addition, antioxidative effects of KAE were done by colorimetric assays. Cell viability and the antioxidative effects such as DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP) were analyzed. ILC induction decreased cell viability in a dose-dependent manner, and the $XTT_{90}$ value (low cytotoxicity value) and $XTT_{50}$ value (high cytotoxicity value) were determined during ILC induction for 15 and 40 minutes, respectively. The butylated hydroxytoluene (BHT) antioxidant significantly increased cell viability damaged by the ILC-induced cytotoxicity. In the protective effect of KAE on ILC-induced cytotoxicity, KAE protected the ILC-induced cytotoxicity by the significant increase of cell viability, and also it showed DPPH-radical scavenging ability, SOD-like ability and inhibitory ability of LP. From these results, it is suggested that ILC induction showed cytotoxicity in these cultures and the oxidative stress is involved in the ILC-induced cytotoxicity. While, KAE prevented ILC-induced cytotoxicity by antioxidative effects. In conclusion, natural products like KAE may be a putative therapeutic agent for the treatment of disease associated with oxidative stress such as ischemia.