International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Deletion of Superoxide Dismutase Gene of Bombyx mori Nuclear Polyhedrosis Virus Affects Viral DNA Replication

  • Wang, Wenbing (Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Key Laboratory of Silkworm Biotechnology, of the Ministry of Agriculture) ;
  • Song, Zhixiu (Institute of Life Sciences, Jingsu University) ;
  • Ji, Ping (Institute of Life Sciences, Jingsu University) ;
  • Wu, Jun (Shanghai Institute of Biochemistry, Chinese Academy of Sciences) ;
  • Zhang, Zhifang (Institute of Life Sciences, Jingsu University) ;
  • He, Jialu (Institute of Life Sciences, Jingsu University) ;
  • Wu, Xiangfu (Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Key Laboratory of Silkworm Biotechnology, of the Ministry of Agriculture)
  • Published : 2004.12.01
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Abstract

Superoxide dismutase (SOD) is an important enzyme which catalyzes superoxide radicals to hydrogen peroxide. A Cu, Zn sod-like gene was found in Bombyx mori nuclear polyhedrosis virus encoding 151 amino acids. To demonstrate its function, a recombinant virus named dsBmNPV with deleted sod gene was constructed. It was discovered that the sod gene was not essential for viral replication. Studies on growth of budded virus in BmN cells and superoxide dismutase and catalase activities in vivo after dsBmNPV infection showed that the titer of dsBmNPV decreased obviously comparing to wild type BmNPV, the sod gene was effective on genomic DNA replication of baculovirus, the peak of SOD activity of silkworm infected with wt-BmNPV appeared between 36 and 48 hrs post infection, and with dsBmNPV, it did not appear. And the changes of CAT activity after infection were similar to SOD activity.

Keywords

References

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