• 생명과학회지
• /
• 제20권11호
• /
• pp.1675-1682
• /
• 2010

해조류 감태의 기능성을 증명하기 위하여 열수 추출하여 여러 가지 생리활성에 대하여 조사하였다. 우선, 감태열수 추출물의 항산화능을 조사하기 위하여 DPPH radical 소거능, SOD 유사활성, XO 저해능을 측정하였다. 그 결과 감태 열수 추출물의 농도가 증가 할수록 높은 항산화능이 나타났으며 항산화제로 잘 알려져 있는 BHA, VitC, catechin의 활성보다 높거나 유사하게 보였다. 아질산염 소거능 측정 실험에서 positive control인 VitC와 감태 열수 추출물은 시료농도가 증가할수록 pH가 낮을수록 증가하였다. 특히, pH 1.2에서 감태 열수 추출물의 경우 VitC 보다 약간 높은 아질산염 소거능을 나타내었다. 감태 열수 추출물의 숙취해소 효능은 ADH와 ALDH 활성증진에 감태 열수 추출물이 미치는 영향을 조사함으로써 증명하고자 하였다. 그 결과, 감태 열수 추출물은 알콜과 acetaldehyde 분해능을 모두 가지고 있을 뿐만 아니라 acetaldehyde의 분해능은 상당히 높게 나타났다. 감태 열수 추출물의 미용효능은 tyrosinase와 elastaase 억제 효과를 분석함으로써 증명하고자 하였다. Tyrosinase 저해능 분석에서 positive control로 사용한 kojic acid는 0.1 mg/ml의 농도에서 65%의 tyrosinase 억제효과를 나타낸 반면, 감태 열수 추출물의 경우 10 mg/ml의 농도에서 58%의 저해효능을 보였다. 비록, 그 효능이 충분히 높지는 않지만 감태 열수 추출물의 농도가 증가함에 따라서 tyrosinase 저해활성이 증가하므로 충분한 미백효능을 기대할 수 있을 것으로 판단된다. Elastase 억제 효능 분석에서는 감태 열수 추출물 0.5 mg/ml에서 38%, 1 mg/ml에서 44%의 저해활성이 나타났다. Positive control로 elastase 저해 활성 효과가 알려진 VitC는 1 mg/ml의 농도에서 36%의 저해활성이 나타났고 elastase 활성저해제인 oleanolic acid는 농도 0.5 mg/ml에서 elastase를 32% 정도 저해하는 것으로 알려져 있으므로 주름 예방 효능에 있어서 감태 열수 추출물의 우수한 기능성이 예상된다.

• 동아시아식생활학회지
• /
• 제22권5호
• /
• pp.657-666
• /
• 2012

The purpose of this study was to improve the quality of Korean traditional sauce products by producing short-term green gochujang with cheongyang pepper powder. To determine its antioxidant compounds and activities, we examined vitamin C, capsaicinoid, total phenolic, and total flavonoid contents, as well as electron-donating, SOD-like, ferrous ion-chelating, and nitrite-scavenging activities. Vitamin C content of short-term green gochujang was higher in CON-M than in the sample, whereas capsaicinoid content increased as the amount of cheongyang pepper powder increased. Total phenolic content, total flavonoid content, electron-donating activity, and nitrite-scavenging activity were higher in the sample than in CON-M, and the contents increased as the amount of meju powder increased. Green gochujang containing the highest amount of cheongyang pepper powder showed the highest SOD-like, and ferrous-ion chelating activities as well as superior nutrient contents, compared to red gochujang.

• 한국식품과학회지
• /
• 제39권4호
• /
• pp.452-457
• /
• 2007

본 실험은 백합의 생리활성 기능을 탐색하여 기능성 식품의 원료로 활용하기 위해 구근, 잎, 꽃을 3부분으로 구분하여 부위별 추출물의 총 페놀함량, 전자 공여능, SOD 유사활성, hydroxyl radical scavenging activity 및 항균성을 측정하였다. 백합 부위별 총 페놀함량에서는 L. davidii 잎 0.068 mg/mL, L. lancifolium 구근 0.039 mg/ml로 나타났다. 플라보노이드 함량은 L. davidii 잎 0.72 mg/mL, L. lancifolium 구근 0.65 mg/ml로 측정되어 백합 품종 및 부위에 따라 페놀성 화합물의 함량에 큰 차이가 있었다. 백합 에탄올 추출물의 SOD 유사활성 측정결과 L. davidii 의 잎 82.7 ${\pm}$ 2.8%, 꽃 66.1 ${\pm}$ 6.7% 구근 50.6 ${\pm}$ 2.0%로 잎에서 가장 높았다. L. lancifolium 에탄올 추출물은 잎 74.9 ${\pm}$ 3.5%, 꽃 73.9 ${\pm}$ 4.1% 및 구근 60.0 ${\pm}$ 4.7%로 나타났다. 백합 부위별 에탄올 추출물의 hydroxyl radical 소거능 측정결과 L. davidii 에탄올 추출물의 소거능은 잎 81.8 ${\pm}$ 2.7%, 꽃 81.4 ${\pm}$ 4.6%, 구근 80.9 ${\pm}$ 7.1% 이었고 L. lancifolium 추출물은 잎 76.4 ${\pm}$ 4.6%, 꽃 81.4 ${\pm}$ 4.6%, 구근 84.5 ${\pm}$ 1.2%로 나타나 품종별, 부위별로 hydroxyl radical 소거능에 큰 차이는 나타나지 않았다. L. davidii 추출물의 전자 공여능 측정 결과 잎 24.9 ${\pm}$ 5.6%, 꽃 14.9 ${\pm}$ 0.4%, 구근 13.798 ${\pm}$ 8.0% 이었고 L. laneifolium 추출물은 잎 15.1 ${\pm}$ 0.7%, 꽃 17.3 ${\pm}$ 0.0%, 구근 13.3 ${\pm}$ 0.2%의 순으로 나타났다. 또한 백합 부위별 추출물을 이용한 그램 양성 균주에서 항균효과 측정 결과는 B. subtilis와 S. enteritidis 에서도 L. davidii, L. lancifolium 의 잎과 꽃에서 10 mm 이상의 clear zone을 형성하였고, 특히 B. subtilis에서는 L. lancifolium 의 꽃 부위에서 항균효과가 높았다. 또한 Gram 음성 균주에서는 Listeria monocytogenes 및 E coli 에서 L. davidii, L. laneifolium의 잎과 꽃에서 10mm 이상의 clear zone을 형성하였으며, L. davidii의 꽃과 L. laneifolium의 꽃, 잎에서 12mm 이상의 높은 항균효과를 나타내었다.

• Journal of Applied Biological Chemistry
• /
• 제54권1호
• /
• pp.33-40
• /
• 2011

Ionizing radiation can be used to improve the color of green tea extract to brighter. As a result, the irradiated green tea extract can be applied easier and broader in food or cosmetic industry. To confirm the retention of the biological activities of the cosmetic products added with green tea polyphenols (PPs), the real cosmetic products including a skin lotion (PS) and an essence (PE) cream were manufactured. Irradiation also applied to the manufactured cosmetic products to see their improvement of color and changes of biological activity. The PP showed 72% of electron donating ability (EDA) at a 5 ppm concentration and the PS and PE containing 2% PP showed higher than 60%, which was similar inhibition activity to vitamin C. The inhibition of superoxide dismutase (SOD)-like activity of PP, PS, and PE were higher than 55% at a 500 ppm concentration and the inhibition of xanthine oxidase (XOase) were also higher than 65% at a 200 ppm concentration. The measurement of lipid oxidation by addition of $Cu^{2+}$ and $Fe^{2+}$ as prooxidants showed that PP and PS had higher metal chelating ability for $Fe^{2+}$ than that of PE and the ability increased by increase of polyphenol concentration isolated from green tea. The $Cu^{2+}$ chelating ability of PP and PS showed higher than 90% at a 200 ppm concentration. Therefore, it is concluded that addition of PP in manufacturing PS and PE retains its biological activities including EDA, inhibition of XOase and SOD-like activity, and metal chelating ability in the manufactured cosmetic products. In addition, irradiation of PS and PE improved color of the products containing PP brighter without any adverse changes in biological activity of the products.

• 대한본초학회지
• /
• 제30권1호
• /
• pp.11-18
• /
• 2015

Objectives : The objective of this study is to evaluate antioxidant activity and the main component content change of steaming-drying fermented Gastrodiae rhizoma extract. Methods : The antioxidant activities were determined for total polyphenol, flavonoid contents, SOD-like activity, electron donating activity, nitrite scavenging ability and major functional components(gastrodin and 4-hydroxybenzyl alcohol content) were also measured. Results : The polyphenol content of fermented Gastrodiae rhizoma by S. cerevisiae were higher than those of fermented Gastrodiae rhizoma by A. oryzae, and when the fermentation period is extended. SOD like activity of fermented Gastrodiae rhizoma showed to be increased by fermentation with S. cerevisiae than fermentation by A. oryzae. Electron donating activity of fermented Gastrodiae rhizoma were increased at almost parallel level as vitamin C, by fermentation. Notably, fermentation by A. oryzae was moderately better than fermentation by S. cerevisiae. Flavonoid content of Gastrodiae rhizoma showed to increasing by fermentation, particularly fermentation by S. cerevisiae was proven to be more effective than by A. oryzae. The more steaming-drying or increased period of fermentation would be resulted in more gastrodin contents but under the same conditions, 4-hydroxybenzyl alcohol content of fermentation by A. oryzae in case of steaming and drying 1 time and 3 time was higher than control. Conclusions : These results has strongly hint the possible applicability of fermentation might be effective to improve the diverse biological activities of Gastrodiae rhizoma and may further supports to develop a functional food materials.

• Nutrition Research and Practice
• /
• 제6권4호
• /
• pp.277-285
• /
• 2012

The purposes of this study were to determine phenolic compounds and to evaluate antioxidant activities of plums (Soldam, Oishiwase and Formosa). Soldam contains the highest amount of total phenolics among cultivars (Formosa: 4.0%, Oishiwase: 3.3%, Soldam: 6.4% for total phenolic) as well as the total flavonoids of which constituents were mainly myricetin and anthocyanidin. The antioxidant activities were measured by DPPH, ABTS radical scavenging, and SOD-like activities. The DPPH radical scavenging activity of Korean plum extracts (200 ${\mu}g/mL$) showed more than 43%, and the Soldam turned out to be the highest : ID50 value: 160-177 ${\mu}g/mL$ for Formosa and Oishiwase; 58-64 ${\mu}g/mL$ for Soldam. The ABTS radical scavenging activity of Korean plum extracts (200 ${\mu}g/mL$) was found to be more than 50%. The SOD-like activity of Korean plum extracts (200 ${\mu}g/mL$) showed more than 70%. Among three kinds of cultivars, Soldam had the highest antioxidant activity. The nitrite scavenging activity of Soldam was 61.5%, which is the highest, compared with that of the other cultivars, about 50%. From these results, Korean plums turned out to be phytochemical rich fruit as well as to show high antioxidant activities.

• 한방재활의학과학회지
• /
• 제19권2호
• /
• pp.89-102
• /
• 2009

Objectives : This study was carried out to understand the immunity responses and anti-oxidation effect of the Gamidaeganghwal-tang(GDT) on rheumatoid arthritis by using the THP-1 cells and the serum of CIA mice. Methods : For this purpose, GDT was orally administerd to mice with rheumatoid arthritis induced by collagen II. To investigate the immunity responses, value of cytokine and gene expression in the THP-1 cell, levels of cytokines in the serum of CIA(collagen type II induced arthritis) mice, number of immunocyte in PBMC of CIA mice were measured. Then, anti-oxidant activity, scavenging activity on DHHP(2,2-diphenyl-1-picrylhydrazyl) free radical and SOD(Superoxide dismutae)-like activity of GDT was observed. Results : 1. The levels of IL-$1{\beta}$, IL-6, IL-8, MCP-1 at 100, $50{\mu}g/m{\ell}$ of GDT were significantly reduced in the THP-1 cell. 2. The levels of TNF-${\alpha}$, COX-2 mRNA expression at 100, $50{\mu}g/m{\ell}$ of GDT and IL-$1{\beta}$, IL-6 at $100{\mu}g/m{\ell}$ of GDT were significantly reduced in the THP-1 cell line. 3. The levels of IL-6, TNF-${\alpha}$ and IL-$1{\beta}$ were significantly reduced in the serum of CIA mice. 4. The absolute number of CD3+, CD4+ and CD8+ cells were significantly induced, CD3+/CD69+, CD3+/CD49+, CD19+, B220+/CD23+ cells were significantly reduced in PBMC. 5. Scavenging activity on DPPH free radical and SOD-like activity were significantly induced in a concentration dependent manner. Conclusions : Taking all these observations, GDT considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

• 대한본초학회지
• /
• 제23권1호
• /
• pp.1-8
• /
• 2008

Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.

• 대한본초학회지
• /
• 제28권3호
• /
• pp.45-51
• /
• 2013

Objectives : This study was conducted to measure the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica against various species of anaerobic bacteria. Methods : In order to evaluate the antioxidative and antimicrobial activities of water- and ethanol-extracts, DPPH radical scavenging and superoxide dismutase (SOD)-like activities were measured in various species such as Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes that induce skin inflammation. Also the total amount of phenol in each water- and ethanol-extract was measured to identify its role in the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica. The antibacterial activity of the extracts was measured by confirming the lowest concentrations in disk diffusion and minimum inhibitory concentration (MIC) tests. Results : It was observed that RJE (Rhus javanica-EtOH extracts) show the highest content of total phenol. In addition, for RJE the total phenol content was higher from samples taken from domestic sources than from samples taken from foreign sources. DPPH radical scavenging activity was increased by treatment with PGE (Punica granatum-EtOH extracts), RJE and RJW (Rhus javanica-water extracts). It was observed that SOD-like activity was highest in the treatment with PGE. All of the extracts showed antimicrobial activity on S. epidermidis, S. aureus, P. acnes and E. coli, including those from Punica granatum, and it was noted that the activity was higher with RJE than with RJW. Conclusions : These results provide evidence that ethanol extracts of Punica granatum and Rhus javanica may have a beneficial role as antioxidants and antibiotics. Extracts from domestic samples of Betula platyphylla var japonica appeared to have a greater efficacy than extracts from foreign samples.

• 한국식품과학회지
• /
• 제34권1호
• /
• pp.118-122
• /
• 2002

전통 민속주의 이화학적 특성과 생리 기능성을 조사하였다. 이들의 조단백질 함량은 $0.15{\sim}10.97%$이었고 pH는 $3.5{\sim}5.0$이었으며 경기도 YA 민속주가 약 14%의 당을 함유하고 있었다. 또한 ACE 저해 활성은 전라도 SO-주가 87.2%로 가장 높았고 혈전 용해활성은 경기도 HO-주가 가장 높았다. 전자공여능과 SOD 유사활성은 각각 충청도 SA-주 85.5%와 경기도 OK-주 70.7%이었으며 tyrosinase 저해 활성은 경상도 KO-주와 전라도 TO-주 및 경기도 CH-주에서 높았으며 아질산염 소거활성은 전라도 TO-주에서 높았다.