Antioxidant Compounds and Activities of Short-term Green Gochujang

단기속성 청고추장의 항산화 성분 및 항산화 활성

  • Shin, Kyung-Eun (Dept. of Culinary & Food Service Management, Kyung Hee University) ;
  • Choi, Soo-Keun (Dept. of Culinary & Food Service Management, Kyung Hee University) ;
  • Kim, Dong-Seok (Dept. of Food Service Industry, Seowon University) ;
  • Kim, Ha-Yun (Dept. of Agrofood Resources, NAAS, RDA)
  • 신경은 (경희대학교 조리.외식경영학과) ;
  • 최수근 (경희대학교 조리.외식경영학과) ;
  • 김동석 (서원대학교 외식산업학과) ;
  • 김하윤 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2012.08.20
  • Accepted : 2012.10.23
  • Published : 2012.10.31

Abstract

The purpose of this study was to improve the quality of Korean traditional sauce products by producing short-term green gochujang with cheongyang pepper powder. To determine its antioxidant compounds and activities, we examined vitamin C, capsaicinoid, total phenolic, and total flavonoid contents, as well as electron-donating, SOD-like, ferrous ion-chelating, and nitrite-scavenging activities. Vitamin C content of short-term green gochujang was higher in CON-M than in the sample, whereas capsaicinoid content increased as the amount of cheongyang pepper powder increased. Total phenolic content, total flavonoid content, electron-donating activity, and nitrite-scavenging activity were higher in the sample than in CON-M, and the contents increased as the amount of meju powder increased. Green gochujang containing the highest amount of cheongyang pepper powder showed the highest SOD-like, and ferrous-ion chelating activities as well as superior nutrient contents, compared to red gochujang.

Keywords

References

  1. Park Mi Jin (2004) Research about ascorbid aicd and capsicin of red pepper from Korea. MS Thesis Yeungnam University, Gyeongsan. p 25.
  2. Ahn In Sook (2007) In vitor antiobesiy effect of kochujang and isolation and identification of active compounds. Ph D Dissertation Pusan National University, Pusan. pp 119-129.
  3. 유종옥, 최원석, 이웅수 (2009) GC-MS 분석에 의한 고추 품종별 및 시판 고춧가루의 capsaicin 및 dihydrocapsacin 함량조사. Food Engineering Progress 13: 38-43.
  4. 윤태인 (2000) 청양 풋고추의 시설재배 기술. 안동대학교 농업개발원. p 320.
  5. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  6. Chiang GH (1986) HPLC analysis of capsaicins and simultaneous determination of capsaicins and piperine by HPLCECD and UV. J Food Sci 51: 499-505. https://doi.org/10.1111/j.1365-2621.1986.tb11165.x
  7. Cho YS, Cho MC, Suh HD (2000) Current status and projects of national hot pepper industry in Korea. J Korean Capsicum Res Coop 6: 1-27.
  8. Choi HS, Lee SY, Baek SY, Koo BS, Yoon HS, Park HY, Yeo SH (2011) Quality characteristics of buckwheat (Fagopyrum esculentum) soksungjang. Korean J Food Sci Technol 43: 77-82. https://doi.org/10.9721/KJFST.2011.43.1.077
  9. Choo JJ, Shin HJ (2000) Sensory evaluation and changes in physicochemical properties and microflora and enzyme activities of pumpkin-added kochujang. Korean J Food Sci Technol 32: 851-859.
  10. Choi JY, Lee TS (2003) Characteristics of volatile flavor compounds in kochujang prepared with commercial enzyme during fermentation. J Korea Soc Agric Chem Biotechnol 46: 207-213.
  11. Choi SK, Shin KE, Jung HA, Park MR (2010). Quality characteristics of Kochujang prepared with soy powder. J East Asian Soc Dietary Life 20: 759-768.
  12. Folin O, Denis W (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
  13. Gray JI, Dugan LR Jr (1975) Inhibition of N-nitrosamine formation in model food system. J Food Sci 40: 981-985 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  14. Jeong EJ, Bang BH, Kim KP (2005) The characteristics of kimchi by the degree of hotness of powdered red pepper. Korean J Food & Nutr 18: 88-93.
  15. Jeong EJ, Seo JS, Bang BH (2007) A study on the kimchi made with green pepper powder. Korean J Food & Nutr 20: 63-67.
  16. Jeong JH (1998) Quality changes of fresh green pepper paste during storage. Korean J Food & Nutr 11: 216-220.
  17. Hwang IG, Kim HY, Hwang Y, Jeong HS, Yoo SM (2011) Quality characteristics of wet noodles combined with cheongyang hot pepper (Capsicum annuum L.) juice. J Korean Soc Food Sci Nutr 40: 860-866. https://doi.org/10.3746/jkfn.2011.40.6.860
  18. Hwang IG, Hwang Y, Kim HW, Lee JS, Jung HS, Yoo SM (2011) Quality characteristics of tofu (soybean curd) added with cheongyang hot pepper (Capsicum annuum L.) juice. J Korean Soc Food Sci Nutr 40: 999-1005. https://doi.org/10.3746/jkfn.2011.40.7.999
  19. Hasimoto F, Nonaka GI, Nishioka I (1989) Tannins and related compound from oolong tea. Chem Pharm Bull 37: 3255-3263. https://doi.org/10.1248/cpb.37.3255
  20. Heo JC, Lee KY, Lee BG, Choi SY, Lee SH, Lee SH (2010) Anti-allergic activities of ultra-fine powder from persimmon. Kor J Food Preserv 17: 145-150.
  21. Howard LR, Smith RT, Wagner AB, Villalon B, Burns EE (1994) Provitamin A and ascorbic acid content of fresh pepper cultivars (Capsicum annuum L.) and processed jalapenos. J Food Sci 59: 362-365. https://doi.org/10.1111/j.1365-2621.1994.tb06967.x
  22. Huffman VL, Schadle ER, Villalon B, Burns. EE (1978) Volatile components and pungency in fresh and processed Jalapeno peppers. J Food Sci 4: 1809.
  23. Hwang IG, Kim HY, Hwang Y, Jung HS, Yoo SM (2011) Quality characteristics of wet noodles combined with cheong yang hot pepper (Capsicum annuum L.) juice. J Korean Soc Food Sci Nutr 40: 860-866. https://doi.org/10.3746/jkfn.2011.40.6.860
  24. Kang NJ, Kwon JK, Lee HC, Jeong HB, Kim HT (2003) Antioxidant enzymes as defense mechanism against oxidative stress induced by chilling in Cucurbita ficifolia leaves. J. Kor Soc Hort Sci 44: 605-610.
  25. Kang, YH, Park YK, Lee GD (1996) The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239.
  26. Kim HS, Ku KM, Suh JK, Kang YH (2009) Quinone reductase inductive activity and growth inhibitory effect against hepatoma cell of oriental melon extract. J Bio-Environm Cont 18: 448-453.
  27. Kim JS (2005) Universalizing Korean food. Korean J Food Culture 20: 499-507.
  28. Kim KS, Park JB, Kim S (2007) Quality characteristics of kochujang prepared with Korean single-harvested pepper (Capsicum annuum L.) 36: 769-765. https://doi.org/10.3746/jkfn.2007.36.6.759
  29. Kim MH, Im SS, Yoo YB, Kim GE, Lee JH (1994) Antioxidative materials in domestic meju and doenjang 4. Separation of phenolic compounds and their antioxidative activity. J Korean Soc Food Nutr 23: 792-798.
  30. Kim MR, Kim HC (2009). A study on the consumption behaviors regarding red pepper paste according to the food-related lifestyles of housewives. J East Asian Soc Dietary Life 19: 1-8.
  31. Kim S, Kim KS, Park JB (2006) Changes of various chemical components by the difference of the degree of ripening and harvesting factors in two single-harvested peppers (Capsicum annuum L.). Korean J Food Sci Technol 38: 615-620.
  32. Kim S, Park JH, Wang IK (2002) Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. J Food Sci 67: 2957-2961. https://doi.org/10.1111/j.1365-2621.2002.tb08845.x
  33. Kim SM, Cho YS, Sung SK (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632.
  34. Ku KH, Kim NY, Park JB, Park WS (2001) Characteristics of color and pungency in the red pepper for kimchi. Korean J Food Sci Technol 33: 231-237.
  35. Labuza TP (1971) Kinetics of lipid oxidation in foods. CRC Crit Rev Food Technol 2: 335-405.
  36. Lee CH, Shin SL, Kim NR, Hwang JK (2011) Comparison of antioxidant effects of different Korean pear species. Korean J Plant Res 24: 253-259. https://doi.org/10.7732/kjpr.2011.24.2.253
  37. Lee GD, Chang HG, Kim HK (1997) Antioxidative and nitrite- scavenging activities of edible mushrooms. Korean J Food Sci Technol 29: 432-436.
  38. Lee K, Suzuki T, Kobashi M, Hasegawa K, Lwai K (1976) Quantitative microanalysis of capsaicin, dihydrocapsaicin and nordihydrocapsaicin using mass fragmentography. J Chromatogy. 123: 119. https://doi.org/10.1016/S0021-9673(00)81108-5
  39. Lee SH, Hong IJ, Park HG, Jew SS, Kim KT (2003) Functional characteristics from the barley leaves and its antioxidant mixture. J Kor Soc Agric Chem Biotechnol 46: 333-337.
  40. Lim JA, Yun BW, Beak SH (2007) Antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Kor J Medicinal Crop Sci 15: 183-188.
  41. Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L (1994) The nitric oxide scavenging property of Ginkgo biloba extract EGb 761. Biochem Biophys Res Comm 201: 748-755. https://doi.org/10.1006/bbrc.1994.1764
  42. Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474.
  43. Materska M, Piacente S, Stochmal A, Pizza C, Olezek W, Peruka I (2003) Isolation and structure elucidation of flavonoid and phenolic acid glycosides from pericarp of hot pepper fruit Capsicum annuum L. Phytochemistry 63: 893-898. https://doi.org/10.1016/S0031-9422(03)00282-6
  44. Matsumoy T, Miyawaki C, Ue H, Yuasa T, Miyatsuji A, Moritani T (2000) Effecgts of capsaicin-containing yellow curry sauce on sympathetic nervous system activity. J Nutr Sci Vitaminol (Tokyo). 46: 309-315. https://doi.org/10.3177/jnsv.46.309
  45. National Rural Resources Development Institute, RDA (2006) Food composition table. 7th ed. National Rural Resources Development Institute, R.D.A, Suwon, Korea. 104-105.
  46. Park CS, Oh EH, Jeong HS, Yoon HS (2009) Quality characteristics of the germinated brown rice wine added with pepper. J Korean Soc Food Sci Nutr 38: 1090-1096. https://doi.org/10.3746/jkfn.2009.38.8.1090
  47. Park CS, Kim KS, Noh JG, Rho CW, Yoon HS (2010) Quality characteristics of the germinated brown rice vinegar added red pepper. J Korean Soc Food Sci Nutr 39: 567-572. https://doi.org/10.3746/jkfn.2010.39.4.567
  48. Park Y, Choi SH, Kim SH, Jang YS, Han J, Chung HG (2008) Functional composition and antioxidant activity from the fruits of Rubus coreanus according to cultivars. Mokchae Konghak 36: 102-109.
  49. Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H (1996) Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. J Agric Food Chem 44: 37-41. https://doi.org/10.1021/jf950190a
  50. Seo SJ, Choi Y, Lee SM, Kong S, Lee J (2008) Antioxidant activities and antioxidant compounds of some specialty rices. J Korean Soc Food Sci Nutr 37: 129-135. https://doi.org/10.3746/jkfn.2008.37.2.129
  51. Shin KE, Choi SK (2011) A study on the development of short-term green gochujang by using chengyang green pepper powder & meju powder. J East Asian Soc Dietary Life 21: 844-852.
  52. Shin KE, Choi SK, Choi IS (2011) Quality characteristics of improvement green gochujang prepared from chengyang pepper powder and roasted soy powder. The Korean Journal of Culinary Research 17: 307-315.
  53. Sung JM, Han YS, Jung JW (2010) Quality characteristics of semi-dried red pepper during frozen storge. Korean J Food Preserv 17: 1-8.
  54. The Korean Society of Food Science and Nutrition (2000) Handbook of experiments of food science and nutrition. Hyoil Co., Seoul. p 257.
  55. Verpoorte RA, Contin J, Memelink (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1: 13-25. https://doi.org/10.1023/A:1015871916833
  56. Vincent KA, Ken AB (1987) Rapid sample preparation method for oleoresins. J Agric Food Chem 35: 777-779. https://doi.org/10.1021/jf00077a032
  57. Woo JH, Shin SL, Jung HS, Lee CH (2010) Antioxidant effect of extracts obtained from three Chrysanthemum species. J Korean Soc Food Sci Nutr 39: 631-636. https://doi.org/10.3746/jkfn.2010.39.4.631
  58. Yoon J, Jun JJ, Lim SC, Lee KH, Kim HT, Jeong HS, Lee J (2010) Changes in selected components and antioxidant and antiproliferative activity of peppers depending on cultivation. J Korean Soc Food Sci Nutr 39: 731-736. https://doi.org/10.3746/jkfn.2010.39.5.731