• Title/Summary/Keyword: SNU-17

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Hepatitis B virus X Protein Promotes Liver Cancer Progression through Autophagy Induction in Response to TLR4 Stimulation

  • Juhee Son;Mi-Jeong Kim;Ji Su Lee;Ji Young Kim;Eunyoung Chun;Ki-Young Lee
    • IMMUNE NETWORK
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    • v.21 no.5
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    • pp.37.1-37.17
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    • 2021
  • Hepatitis B virus X (HBx) protein has been reported as a key protein regulating the pathogenesis of HBV-induced hepatocellular carcinoma (HCC). Recent evidence has shown that HBx is implicated in the activation of autophagy in hepatic cells. Nevertheless, the precise molecular and cellular mechanism by which HBx induces autophagy is still controversial. Herein, we investigated the molecular and cellular mechanism by which HBx is involved in the TRAF6-BECN1-Bcl-2 signaling for the regulation of autophagy in response to TLR4 stimulation, therefore influencing the HCC progression. HBx interacts with BECN1 (Beclin 1) and inhibits the association of the BECN1-Bcl-2 complex, which is known to prevent the assembly of the pre-autophagosomal structure. Furthermore, HBx enhances the interaction between VPS34 and TRAF6-BECN1 complex, increases the ubiquitination of BECN1, and subsequently enhances autophagy induction in response to LPS stimulation. To verify the functional role of HBx in liver cancer progression, we utilized different HCC cell lines, HepG2, SK-Hep-1, and SNU-761. HBx-expressing HepG2 cells exhibited enhanced cell migration, invasion, and cell mobility in response to LPS stimulation compared to those of control HepG2 cells. These results were consistently observed in HBx-expressed SK-Hep-1 and HBx-expressed SNU-761 cells. Taken together, our findings suggest that HBx positively regulates the induction of autophagy through the inhibition of the BECN1-Bcl-2 complex and enhancement of the TRAF6-BECN1-VPS34 complex, leading to enhance liver cancer migration and invasion.

Polarimetry of solar system small bodies using the Seoul National University 61cm telescope and TRIPOL

  • Jin, Sunho;Ishiguro, Masateru;Kwon, Yuna Grace;Geem, Jooyeon;Bach, Yoonsoo P.;Seo, Jinguk;Sasago, Hiroshi;Sato, Shuji
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.1
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    • pp.64.2-64.2
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    • 2019
  • It is known that lights scattered by comets and asteroids are partially polarized. From polarimetric observations of those objects, we can investigate physical properties, such as albedos, sizes of cometary dust particles and regolith of asteroids. Since the polarization degrees of those objects highly depend on their phase angles (Sun-object-observer's angles), long-term monitoring observations are required. Moreover, comets show unforeseeable activations (i.e., outbursts) which need follow-up observations to understand the mechanism. In order to realize such monitoring and transient observations, we installed the Triple-Range Imager and POLarimeter (TRIPOL) on the 61cm telescope of Seoul National University (Hereafter, SNU) Gwanak campus. With this combination, we can obtain g', r', i' bands photopolarimetric images simultaneously with $8.0^{\prime}{\times}8.0^{\prime}$ field of view and pixel resolution of 0.94" pixel-1. Here, we make a presentation regarding the photometric and polarimetric performances of TRIPOL on the SNU 61cm telescope. In addition, we introduce initial polarimetric results of asteroid and comets with the instruments. First, we determine the limiting magnitudes (defined as magnitudes for S/N=5) of $15.17{\pm}0.06$ (g'-band), $15.68{\pm}0.01$ (r'-band), $16.24{\pm}0.03$ (I'-band), respectively, with total 240-seconds exposure (four 60-seconds exposure images, each was taken at different rotation angle for the half-wave plate). Second, we found that the instrumental polarization is negligibly small, ($-0.32{\pm}0.04%$ in the g', $-0.36{\pm}0.05%$ in the r' and $-0.21{\pm}0.04%$ in the i'-bands), while the polarization efficiencies are large enough to maximize the performance (i.e., $97.52{\pm}0.03%$ in the g', $98.83{\pm}0.02%$ in the r' and $99.15{\pm}0.02%$ in the i'-bands). With the instruments, we made observations of three Jupiter-family comets, 21P/Giacobini-Zinner, 38P/Stephan-Oterma, and 46P/Wirtanen and plan to observe one near-Earth asteroid, (433) Eros, on a trial basis. Especially for comets, we discriminate signals from dust and gas to eliminate gas contamination, which are known to change observed degree of linear polarization, using multi-band images. We confirm that the phase angle dependency of these comets are consistent with previous observations, probably because polarimetric property of Jupiter-family comets are broadly homogeneous unlike asteroids. We will also describe future observation plans using TRIPOL and SNU 61cm telescope.

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Effect of Amination on the Biological Activity of $\beta$-Glucan from Sangwhang (Phellinus linteus)

  • Bae, In-Young;Shin, Ji-Yoon;Lee, Hyeon-Gyu
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.683-686
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    • 2008
  • $\beta$-Glucans, which were isolated from sangwhang (Phellinus linteus), were subjected to reductive amination and the biological properties of the derivative were investigated. The degree of substitution of the aminated sangwhang $\beta$-glucan was calculated by elemental analysis to be 1.13. Bronchoalveolar lavage (BAL) experiments showed that the aminated derivative increased nitric oxide production. In addition, the amination enhanced in vitro cytotoxic activities against HT1080 and SNU-C2A cell lines. Thus, the aminated derivative is shown to enhance immune systems by the incorporation of amino groups into the polymer structure.

OBSERVATION OF IMPACT SITES OF SHOEMAKER-LEVY 9 COMET (슈메이커-레비 9 혜성의 목성 충돌관측)

  • Lee, Si-Woo;Kim, Seung-Ri;Seong, Hwan-Gyeong;Pyo, Tae-Su
    • Publications of The Korean Astronomical Society
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    • v.10 no.1
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    • pp.1-14
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    • 1995
  • We observed impact spots on Jupiter which were formed by the collision with fragments of Shoemaker-Levy 9 comet during the period from 17 July to 13 August, by using the SNU 61cm reflector combined with CCD camera and DBVRI filters. In order to get more clear image of impact spots, the observed data were processed by the unsharp masking method, high-pass filtering method and Richardson-Lucy algorithm after the basic processing through IRAF package. The sizes of impact spots range from 2000km to 1000km, and from this size, the original size of comet fragments before collision with Jupiter is estimated to be less than ${\sim}3km$. From the observed rotating rate of impact spots, the rotational period of Jupiter is determined as $9^h$ $56^m$ $32^s$ at the southern latitude of $47^{\circ}{\sim}49^{\circ}$.

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Daily Dynamics of Grateful Mood, Emotional Support from Parents, and Psychological Well-Being across Seven Days among Korean Adolescents

  • Chung, Grace H.
    • International Journal of Human Ecology
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    • v.17 no.2
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    • pp.43-51
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    • 2016
  • Even though gratitude is considered as an important virtue to possess for successful adulthood, little is known about what cultivates gratitude and its benefits associated with psychological well-being among adolescents. By examining gratitude as a daily mood, this study asked (1) whether adolescents' daily grateful mood varied as a function of daily parental emotional support and (2) whether adolescents' psychological well-being varied as a function of daily grateful mood within person across seven days. The sample consisted of 70 adolescents in 8th grade recruited from a public middle school. Participants completed daily diary checklists online each evening for seven days. On days when adolescents perceived greater parental emotional support from mother or father than they typically did during the week, they rated their grateful mood to be higher than other days. With more emotional support from father, adolescents reported more grateful mood not only on that day, but also the following day. While this result highlights the important role that parents play in adolescents' experience of grateful mood, emotionally supportive behaviors of fathers appear particularly promising for cultivating gratitude. Further, daily grateful mood on a given day accounted for the significant variance in adolescents' psychological well-being not only on the same day, but also the following day. Research and practical implications of the results are discussed.

Accuracy Evaluation of CT-Based Attenuation Correction in SPECT with Different Energy of Radioisotopes (SPECT/CT에서 CT를 기반으로 한 Attenuation Correction의 정확도 평가)

  • Kim, Seung Jeong;Kim, Jae Il;Kim, Jung Soo;Kim, Tae Yeop;Kim, Soo Mee;Woo, Jae Ryong;Lee, Jae Sung;Kim, Yoo Kyeong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.17 no.1
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    • pp.25-29
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    • 2013
  • Purpose: In this study, we evaluated the accuracy of CT-based attenuation correction (AC) under the conventional CT protocol (140 kVp, on average 50-60 keV) by comparing the SPECT image qualities of different energy of radioisotopes, $^{201}Tl,\;^{99m}Tc$ and $^{131}I$. Materials and Methods: Using a cylindrical phantom, three different SPECT scans of $^{201}Tl$ (70 keV, 55.5 MBq), $^{99m}Tc$ (140 keV, 281.2 MBq) and $^{131}I$ (364 keV, 96.2 MBq) were performed. The CT image was obtained with 140 kVp and 2.5 mA in GE Hawkeye 4. The OSEM reconstruction algorithm was performed with 2 iterations and 10 subsets. The experiments were performed in the 4 different conditions; non-AC and non-scatter correction (SC), only AC, only SC, AC and SC in terms of uniformity and center to peripheral ratio (CPR). Results: The uniformity was calculated from the uniform whole region in the reconstructed images. For $^{201}Tl$ and $^{99m}Tc$, the uniformities were improved by about 10-20% AC was applied, but these were decreased by about 2% as SC was applied. The uniformity of $^{131}I$ was slightly increased as both AC and SC were applied. The CPR of the reconstructed image was close to one, when AC was applied for $^{201}Tl$ and $^{99m}Tc$ scans and $^{131}I$ was distant from 1 and that is only AC. Conclusion: The image uniformity improved by AC on low energy likely to $^{201}Tl$ and $^{99m}Tc$. However, image uniformity of high energy such as $^{131}I$ was improved, when both AC and SC was applied.

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Effect of Planting Date and Hybrid on Forage Yield and Quality of Corn for Silage I. Agronomic characteristics and forage yield of corn (파종시기 및 품종이 사일리지용 옥수수의 수량과 사료가치에 미치는 영향. I. 옥수수의 생육특성 및 사초수량)

  • Kim, J.D.;Kim, D.A.;Park, H.S.;Kim, S.G.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.3
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    • pp.211-220
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    • 1999
  • The corn is mostly planted in mid-April in single-cropping silage system if possible, however, in a double-cropping silage system, the corn planting date is delayed until after the mid-May. The objective of this study was to evaluate agronomic characteristics and forage performance of the eight government recommended corn hybrids for silage at two dates of planting. Split-plot design replicated three times was used, that is, main plots were planting dates, sub-plots were eight hybrids at Suweon in 1997. Days from planting to silking of optimum planting was 84 days and that of late was 69 days. The difference in silking day among the hybrids was 6 days at optimum planting and 2 days at late planting. Plant height of optimum planting corn was higher than that of late one, however, ear height and stem diameter of optimum planting were lower than those of late planting corn. Among the corn hybrids tested, plant heights of 'P3156' and 'P3163' were higher than those of the other hybrids. Ear height of 'DK713' was the lowest among the corn hybrids and stem diameter of 'P3394' was thicker than that of the other hybrids. European corn borer(ECB) damage(16.6%) at optimum planting was lower than that(49.9%) of late, however, there were no significant ECB damage differences among the corn hybrids tested. Whole plant dry matter(DM) contents of 'P3525', 'P3394' and 'P3352' hybrids were higher than those of the other corn hybrids at harvest. Ear percentages of the total dry matter(DM) of 'P3394' and 'P3156' were higher than those of the other corn hybrids. Dry matter(DM) and total digestible nutrients(TDN) yields(19,696 and 14,621kg/ha) at optimum planting were higher than those (17,163 and 12,570kg/ha) of late planting, while there were no significant differences in DM and TDN yields among the corn hybrids tested. And those hybrids with greater proportion of grain in the whole plant have higher DM and TDN yields. Correlation coefficients for days from planting to silking with DM and TDN yields were $0.84^{**}$ and $0.87^{**}$, and those for ear percent were $0.86^{**}$ and $0.87^{**}$. Results of this study indicate that optimum planting date(15 April) is better than late planting date(16 May) in agronomic characteristics and forage production of silage corn. Days from planting to silking and ear percent as welI as other characters are important factors screening the corn hybrid for silage.

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Creative Idea and an Analysis of Fashion Design on Korean Image through the SCAMPER Technique (SCAMPER에 의한 한국적 이미지의 복식디자인 분석 및 창의적 발상)

  • Choi, Sun Young;Kim, Min-Ja
    • Journal of the Korean Society of Costume
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    • v.64 no.1
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    • pp.1-17
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    • 2014
  • The purpose of this study is to suggest the plans, which can creatively utilize Korean images by introducing the SCAMPER technique, one of the techniques used to create ideas in the fashion design process. For the research methods, the case study which collects all the masters' theses relating to the fashion design process applied Korean images for recent 10 years and the literature study through books, masters' theses, academic journals' theses, news articles, and Internet data were done together. As for the result of the study, the level of utilization of Korean images in the researches on Korean fashion designs stayed simple utilization, which used a motif as it is in its original form or changed only the size. Furthermore, of the 39 theses reviewed in the study, 59% of them used less than 3 of the 7 SCAMPER items. Therefore, it could be said that the Korean images are restrictively being used in the fashion design studies. If SCAMPER is to be actively utilized as a design process in the future, it is expected that it will make great contributions to the development of creative Korean fashion design.

Cytotoxic Activity of Methanol Fractions and Solvent Extracts from Houttuynia cordata $T_{HUNS}$ (IX) on Various Cancer Cells (어성초 용매추출물과 메탄올 분획물의 암세포주에 대한 세포독성)

  • Lee Jeong Ho;Baek Seung Hwa;Lim Jin A;Chun Hyun Ja;Lee Ki Nam
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1288-1292
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    • 2003
  • This study was carried out to evaluate cytotoxic effects of Houttuynia cordata T/sub HUNB/ extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 ㎍/㎖ concentration of methanol extract (63.81 %) of Houttuynia cordata T/sub HUNB/ was shown significantly antitoxic activity on A549 cell lines. The order of cytotoxicity fractions of methanol from Houttuynia cordata T/sub HUNB/ extracts against cancer cell lines in vitro is as follows : hexane fraction layer > chloroform fraction layer > ethyl acetate fraction layer > buthanol fraction layer > water fraction layer. These results suggest that the hexane fraction of methanol extract from Houttuynia cordata T/sub HUNB/ extract may be a valuable choice for the development of antitumor agents.

Recirculating Aquaculture System Design and Water Treatment Analysis based on CFD Simulation

  • Juhyoung Sung;Sungyoon Cho;Wongi Jeon;Yangseob Kim;Kiwon Kwon;Deuk-young Jeong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.11
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    • pp.3083-3098
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    • 2023
  • As demands for efficient and echo-friendly production of marine products increase, smart aquaculture based on information and communication technology (ICT) has become a promising trend. The smart aquaculture is expected to control fundamental farm environment variables including water temperature and dissolved oxygen (DO) levels with less human intervention. A recirculating aquaculture system (RAS) is required for the smart aquaculture which utilizes a purification tank to reuse water drained from the water tank while blocking the external environment. Elaborate water treatment should be considered to properly operate RAS. However, analyzing the water treatment performance is a challenging issue because fish farm circumstance continuously changes and recursively affects water fluidity. To handle this issue, we introduce computational fluid dynamics (CFD) aided water treatment analysis including water fluidity and the solid particles removal efficiency. We adopt RAS parameters widely used in the real aquaculture field to better reflect the real situation. The simulation results provide several indicators for users to check performance metrics when planning to select appropriate RAS without actually using it which costs a lot to operate.