• Toxicological Research
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• v.13 no.3
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• pp.247-250
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• 1997

We have studied cytotoxicity of S-nitroso-N-acetyl- N-DL-penicillamine(SNAP), a Nitric oxide (NO)-releasing compound, in RAW264.7 macrophages. SNAP is cytotoxic to RAW264.7 cells in a concentration-dependent manner. PMA(200 nM) stimulated cells to produce superoxide anton radical($O_2^{-\cdot}$) and caused a little loss of RAW264.7 cell viability for 12 hr and diminished the cytotoxicity of SNAP. The mechanism by which PMA can protect cells against NO-mediated cytotoxicity was studied by peroxynitrite-enhanced chemiluminescence method. Observed results suggested that $O_2^{-\cdot}$ produced by PMAstimulated RAW264.7 cells may quench NO released by SNAP and reduce NO, thus attenuating NO-related damages.

• The Journal of Korean Medicine
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• v.24 no.2
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• pp.159-165
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• 2003

Objectives : Coptidis rhizoma (CR) is an oriental medicine that has been used in many traditional prescriptions against diabetes mellitus in Korea for centuries. Our purpose was to determine the protective effect and its action mechanism of CR on the cytotoxicity of pancreatic -cell line (RINm5F cell). Methods : In this experiment, we used methods such as MTT assay for detection of cytotoxicity, DNA fragmentation assay for detection of apoptotic cell death, LDH activity assay for detection of necrotic cell death, and measurement of $DiOC_{6}$ (3) retention for detection of mitochondrial membrane potential (MMP). Background : Nitric oxide (NO) is believed to playa key role in the process of pancreatic -cell destruction leading to insulin-dependent diabetes mellitus (IDDM). Results : Exposure of RINm5F cells to chemical NO donor such as S-nitroso-N-acetylpenicillamine (SNAP) induced cytotoxic events such as DNA fragmentation and lactate dehydrogenase (LDH) release into medium. However, pretreatment of RINm5F cells with CR extract ($10~50{\mu\textrm{g}}/ml$) for 3 hours prevented SNAP-induced DNA fragmentation and LDH release into medium through the inhibition of MMP disruption. Conclusions : These results suggest that CR may be a candidate for a therapeutic or preventing agent against IDDM.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07a
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• pp.392-395
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• 2003

For the first time, the new dual trench gate Emitter Switched Thyristor is proposed for eliminating snap-back effect which leads to a lot of serious problems of device applications. Also, the parasitic thyristor that is inherent in the conventional EST is completely eliminated in the proposed EST structure, allowing higher maximum controllable current densities for ESTs. Moreover, the new dual trench gate allows homogenous current distribution throughout device and preserves the unique feature of the gate controlled current saturation of the thyristor current. The conventional EST exhibits snap-back with the anode voltage and current density 2.73V and $354/{\S}^2$, respectively. But the proposed EST exhibits snap-back with the anode voltage and current density 0.93V and $58A/{\S}^2$, respectively. Saturation current density of the proposed EST at anode voltage 6.11V is $3797A/{\S}^2$. The characteristics of 700V forward blocking of the proposed EST obtained from two dimensional numerical simulations (MEDICI) is described and compared with that of the conventional EST.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.3
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• pp.247-254
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• 2010

Due to lightweight and high durability of glass-fiber reinforced polyester (GFRP) materials, they are promising alternatives to conventional construction materials such as steel, concrete and wood. As good application examples of GFRP materials, several types of temporary arch bridges were suggested and verified by finite element analyses in our previous study where snap-fit GFRP decks were applied. In this paper, we conduct a structural performance test to verify safety and serviceability of the temporary arch bridge, where snap-fit GFRP decks are assembled by bolts. The structural problems occurred in this test are also discussed and improvement of temporary arch bridges is suggested to resolve the occurred structural problems.

• Structural Engineering and Mechanics
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• v.89 no.1
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• pp.13-22
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• 2024

The nonlinear snap-through buckling of functionally graded (FG) carbon nanotube reinforced composite (CNTRC) curved tubes is analytically investigated in this research. It is assumed that the FG-CNTRC curved tube is supported on a three-parameter nonlinear elastic foundation and is subjected to the uniformly distributed pressure and thermal loads. Properties of the curved nanocomposite tube are distributed across the radius of the pipe and are given by means of a refined rule of mixtures approach. It is also assumed that all thermomechanical properties of the nanocomposite tube are temperature-dependent. The governing equations of the curved tube are obtained using a higher-order shear deformation theory, where the traction free boundary conditions are satisfied on the top and bottom surfaces of the tube. The von Kármán type of geometrical non-linearity is included into the formulation to consider the large deflection in the curved tube. Equations of motion are solved using the two-step perturbation technique for nanocomposite curved tubes which are simply-supported and clamped. Closed-form expressions are provided to estimate the snap-buckling resistance of FG-CNTRC curved pipes rested on nonlinear elastic foundation in thermal environment. Numerical results are given to explore the effects of the distribution pattern and volume fraction of CNTs, thermal field, foundation stiffnesses, and geometrical parameters on the instability of the curved nanocomposite tube.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.2 no.2
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• pp.1-5
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• 2001

One of the major advantages of using engineering plastics is ease of part assembly through a locking mechanism known as a snap fit. The typical snap fit involves a short cantilever beam with a projection at the free end. which slides over a one way ramp on the mating part to lock in place. The tightness of the mechanism is determined by the lateral interference of the two sliding members If too small they become loose and can't hold together. while if too large. excessive force can be generated. causing failure of the cantilever beam during the assembly operation. Therefore. the accurate determination of the force-deflection relationship for cantilever beams is a key element in snap fit design. And also. the process of injection molding should be considered when cantilever beam is designed. But it is not easy for novice designers to design them appropriately because of the profound knowledge related to injection molding. In this paper. an intelligent design program has been developed and proposed to improve a conventional empirical design method.

• The Korean Journal of Physiology and Pharmacology
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• v.8 no.3
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• pp.141-146
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• 2004

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins, composed of two presynaptic membrane proteins [synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin] and a presynaptic vesicular protein [vesicle-associated membrane protein (VAMP)], serve as a core of exocytotic fusion machinery, which can be affected by ischemia. Synaptic protein in core region, striatum and cortex has been shown to alter after focal ischemia, however, little is known in hippocampus. Hippocampus is remote from ischemic core, but it is one of the most vulnerable regions. Using immunohistochemistry, the present study was undertaken to investigate the alteration of expression of SNAP-25, syntaxin, and VAMP in the hippocampus of rats which were subjected to middle cerebral artery occlusion (MCAO) for 2h and allowed to reperfuse. At 2 weeks of reperfusion, the SNAP-25 and syntaxin immunoreactivity was increased in the stratum oriens of the CA1 and the stratum lucidum of the CA3 in the ipsilateral hippocampus. However, VAMP immunoreactivity didn't show significant change. These results demonstrate that the level of the presynatpic plasma membrane proteins (SNAP-25 and syntaxin) in the rat hippocampus is more sensitively affected by focal ischemia than that of the synaptic vesicle protein (VAMP).

• Journal of the Korean Society of Propulsion Engineers
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• v.9 no.4
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• pp.89-95
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• 2005

Thrust cut off(TCO) system is installed at the port of a rocket motor case forward dome. The snap ring and the closure are escaped sequentially by pulling out a wedge under internal pressure. The hydraulic structural tests of TCO and numerical simulations were performed, and both results were compared to understand the deformation behavior of TCO. By increasing splines symmetrically, the sealing capacity of TCO can be improved significantly. The escape pressure of TCO increases according to the increase of friction coefficient and there is a critical friction coefficient beyond which the snap ring can not be nearly escaped even after forced escape of wedge. Under low friction coefficient the snap ring is contracted to radial direction and easily escaped. But, under high friction coefficient, the snap ring can not be escaped from the port even after severe plastic deformation.

• Journal of Veterinary Science
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• v.25 no.3
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• pp.48.1-48.12
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• 2024

Importance: Early diagnosis of canine pancreatitis is challenging due to non-specific clinical signs. Currently, abdominal ultrasonography and measurement of canine pancreatic lipase (cPL) have been employed for the diagnosis of pancreatitis. Objective: Many qualitative and quantitative commercial cPL tests have been developed and used in veterinary clinics. This study aimed to compare three different methodologies SNAP cPL, Spec cPL, and Vcheck cPL tests to assess the concordance of these assays. Methods: Fifty serum samples were collected from 36 dogs with or without pancreatitis and subjected to SNAP cPL, Spec cPL, and Vcheck cPL tests. Agreement and correlation coefficients were calculated between the test results, and correlations were determined during the management of the patients. Results: The results of the three cPL assays were strongly correlated in 47/50 serum samples (94%). Cohen's kappa analysis between the Spec cPL and Vcheck cPL showed near perfect agreement (κ = 0.960, p < 0.001), SNAP cPL and Vcheck cPL (κ = 0.920, p < 0.001), and Spec cPL and SNAP cPL (κ = 0.880, p < 0.001). The correlation coefficients (r) between data from Spec cPL and Vcheck cPL tests was calculated by Spearman's correlation test (r = 0.958, p < 0.001). Furthermore, the patterns of change in serum cPL concentrations determined using Spec cPL and Vcheck cPL were significantly consistent during the monitoring period in 11 patients. Conclusions and Relevance: Our data illustrated that Spec cPL and Vcheck cPL tests are compatible for clinical use in the diagnosis and monitoring of canine pancreatitis.

• Restorative Dentistry and Endodontics
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• v.16 no.2
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• pp.197-212
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• 1991

The purpose of this study was to roughness on the polished surface of visible light composite resins and was to observe the polished surfaces under Scanning Electrom Microscope(ISI DS 130 AKASHI Co. JAPAN). The surface roughness tester(Surfcom 700A Seimtsu profilometer Tokyo, Japan) was used to measure roughness of polished surfaces. In this study, 5 brands of visible of composite resins were examined, Pyrofile light bond Anterior Lite fil anterior Photo clearfil anterior & posterior Palfique light Anterior and posterior Silux Anterior. White point, Silicure point, Super snap Sof-Lex medium disk as cutting instrument, and celluloid matrix were used. The results obtained were as follows. 1. The Celluloid matrix produced the smoothest surfaces. 2. The surfaces made by Soflex medium disk was smoother than the surfaces made by any other polishing instruments. 3. The values of surface roughness made by White point, Silicon point and Super snap were similer. 4. Palfique light (AP) visible light composite resin showed the smoothest surface after polishing with Silicone point, Super snap disk and Soflex mediuem disk in all tested materials.