• 한국축산식품학회지
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• 제22권3호
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• pp.240-246
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• 2002

본 연구에서는 돼지 비선호 부위인 후지육을 이용하여 제조된 간장과 고추장 양념육을 tray에 함기 포장한 다음 5$^{\circ}C$와 1$0^{\circ}C$에서 각각 냉장저장하며 품질 특성의 변화와 저장수명을 조사하였다. 간장과 고추장 양념육에서 총균수의 변화는 저장 0일째 각각 5.24와 5.75 log10CFU/$\textrm{cm}^2$ 수준이었다. 그러나 간장 양념육은 5'E에서 10일, 1$0^{\circ}C$에서는 6일 후에, 고추장 양념육은 각각 6일과 4일후에 총균수가 7 log10CFU/$\textrm{cm}^2$을 넘어섰다. 관능검사 결과 5$^{\circ}C$와 1$0^{\circ}C$에서 간장 양념육은 10일과 8일째, 그리고 고추장 양념육은 10일과 6일째 일부 항목에서 3.0 미만의 점수를 받아 상품성을 잃은 것으로 평가되었다. 저장기간이 경과할수록 pH와 수분함량은 감소하는 경향을 보였다. TBA와 VBN값은 저장기간이 연장될수록 증가하는 경향을 보였고 5$^{\circ}C$에서보다 1$0^{\circ}C$에서 더 두드러졌다. 저장 기간 중 포장내 산소 농도는 0일째 간장과 고추장 양념육에서 각각 19.8%와 19.9%에서 점차 감소하였고 이산화탄소 농도는 0%또는 0.7%에서 점차 증가하였다. 이러한 증감의 변화는 5$^{\circ}C$에서보다 1$0^{\circ}C$에서 더욱 두드러지게 나타났다. 상기 결과를 종합하여 볼 때 간장과 고추장 양념육의 최소 유통기한은 5$^{\circ}C$의 경우 두 시료 공히 8일이었고 1$0^{\circ}C$의 경우에는 각각 6일과 4일로 예상되었다. 이보다 저장수명을 연장시키기 위해서는 원료육과 제조시 미생물 오염도를 줄이고 저장온도를 빙점 가까이 유지하며 진공포장을 하는 것이 바람직할 것으로 판단된다.

• Molecules and Cells
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• 제23권3호
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• pp.370-378
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• 2007

A superoxide dismutase was purified 62-fold in seven steps to homogeneity from Methylobacillus sp. strain SK1, an obligate methanol-oxidizing bacterium, with a yield of 9.6%. The final specific activity was 4,831 units per milligram protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. The molecular weight of the native enzyme was estimated to be 44,000. Sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 23,100. The isoelectric point of the purified enzyme was found to be 4.4. Maximum activity of the enzyme was measured at pH 8. The enzyme was stable at pH range from 6 to 8 and at high temperature. The enzyme showed an absorption peak at 280 nm with a shoulder at 292 nm. Hydrogen peroxide and sodium azide, but not sodium cyanide, was found to inhibit the purified enzyme. The enzyme activity in cell-free extracts prepared from cells grown in manganese-rich medium, however, was not inhibited by hydrogen peroxide but inhibited by sodium azide. The activity in cell extracts from cells grown in iron-rich medium was found to be highly sensitive to hydrogen peroxide and sodium azide. One mol of native enzyme was found to contain 1.1 g-atom of iron and 0.7 g-atom of manganese. The N-terminal amino acid sequence of the purified enzyme was Ala-Tyr-Thr-Leu-Pro-Pro-Leu-Asn-Tyr-Ala-Tyr. The superoxide dismutase of Methylobacillus sp. strain SK1 was found to have antigenic sites identical to those of Methylobacillus glycogenes enzyme. The enzyme, however, shared no antigenic sites with Mycobacterium sp. strain JC1, Methylovorus sp. strain SS1, Methylobacterium sp. strain SY1, and Methylosinus trichosproium enzymes.

• The Korean Journal of Physiology and Pharmacology
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• 제4권2호
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• pp.137-142
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• 2000

The physiological roles of brain angiotensin II in mediating water deprivation-induced drinking and in regulating renal renin release were assessed in male Sprague-Dawley rats. Specific $AT_1$ receptor antagonists, losartan and SK 1080, and antisense oligonucleotide (AS-ODN) directed to $AT_1$ receptor mRNA were intracerebroventricularly (i.c.v.) administered in conscious unrestrained rats. When water was given 20 min after i.c.v. injection of $AT_1$ receptor antagonists in 48-h water-deprived rats, losartan and SK 1080 produced approximatly 20% and 50% decrease in 1-h water intake, respectively. In contrast, i.c.v. treatment of the AS-ODN to $AT_1$ receptor mRNA for 24-h did not alter 1-h water intake in 24-h water-deprived rats, but prevented the increase in overnight water intake after 24-h water-deprivation. Six-day i.c.v. treatment of AS-ODN did not alter either the basal plasma renin concentration or renal cortical levels of renin and renin mRNA. The present results suggest that endogenous brain Ang II plays an important role in thirst and water intake through $AT_1$ receptors, but further studies are required to elucidate its regulatory role in renal renin synthesis.

• 한국전자파학회지:전자파기술
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• 제10권1호
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• pp.77-83
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• 1999

전파무반사실을 구축하기 위해서는 많은 비용이 요구되므로 하나의 전자파무반사실을 여러가지 용도로 사용되는 것은, 공간적, 경제적으로 매우 효율적이다. 본 논문에서는 현재 SK텔레콤에서 운용중인 안테나 및 EMC/EMI측정을 위한 다용도 전자파무반사실에 대하여 설명하고 그 성능에 대한 측정결과를 제시한다. 이 전자파무반사실은 150 MHz에서 40 GHz까지의 주파수 범위까지 Far Field와 Near field에 대한 안테나 측정이 가능한 안테나 측정시스템과 측정환경을 보유하고 있으며, EMC/EMI 측정을 위한 ANSI C63.4dhk CISPR 16.1의 요구조건을 모두 만족하고 있다.

• 한국식품영양학회지
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• 제11권6호
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• pp.678-682
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• 1998

A strain of Streptomyces sp. SK-862, isolated from soil in Wonju city, was able to prodce a biologically active substance that has a strong inhibitory activity against proteolsis by trypsin. The inhyibitory substance was extracted by n-butanol, and then purified by the adsorption chromatography followed by the reverse-phase high performacne liquid chromatography. The purified substance was stable over the pH range from 2 to 10, but was unstable when treated at 8$0^{\circ}C$ for 60 min. This substance was soluble in water, methanol, ethanol nd butanol, but insoluble in chlorofrom and ethylacetate. The Rf value of the purified substance on the thin layer chromatography were 0.56 in n-butanol : methanol : water(5 : 3 : 1v/v) solvent system compare dto 0.23 in ethanol : ammonium hydoxide : water(8 : 1 : 1v/v) solvent system. This substance has maximum absorption at 259 nm. The chemical reaction of the substance was negative for sugar but positive for ninhydrine and iodine reaction.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.675-683
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• 2016

One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SK-ENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.

• Archives of Pharmacal Research
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• 제15권4호
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• pp.356-359
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• 1992

The activity-oriented fractionation of Psoralea corylifolia led to an isolation of a (+) bakuchio 1 as an activ eprinciple of its antitumoral property in vitro 1 was observed to exhibit a mild cytotoxicity against five kinds of cultured human cancer cell lines, i. e. the A549, SK-OV-3, SK-MEL-2, XF-498 and HCT15. The synthesized 2, 3-epoxide of (+)-bakuchiol 3 showed the similar activity as the (+) bakuchiol 1, whereas the other oxidation derivatives 4 and 5 including the acetyl (+) bakuchiol 2 showed a decreased activity.

• Natural Product Sciences
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• 제20권2호
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• pp.71-75
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• 2014

Nine triterpenes were isolated from the petroleum ether and MeOH extract of Perilla frutescens var. acuta leaves. Their structures were determined to be arjunic acid (1), maslinic acid (2), oleanolic acid (3), euscaphic acid (4), tormentic acid (5), 3-O-trans-p-coumaroyltormentic acid (6), 28-formyloxy-$3{\beta}$-hydroxy-urs-12-ene (7), ursolic acid (8), and corosolic acid (9) by spectroscopic methods. The compounds 1, 2, 4, 6, and 7 were isolated for the first time from this plant and the Genus Labiatae. The isolated compounds (1-9) were tested for cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15) in vitro using a Sulforhodamin B bioassay.

• 한국식품과학회지
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• 제35권3호
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• pp.479-482
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• 2003

동과자 열수 추출물의 in vitro 항암효과는 2 mg/mL의 농도에서 가장 높게 나타났으며 in vivo 상에서의 0.1 g/kg, 0.01 g/kg의 경구 투여시 각각 21%, 17%의 생명연장율(increased life span, ILS)을 나타내었다. SK-OV-3에 대한 SRB assay를 이용한 열수 추출물(2 mg/mL)의 방사선(5 Gy) 병용 투여에 있어서 86%의 저해효과를 나타내었다.

• Archives of Pharmacal Research
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• 제18권2호
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• pp.118-120
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• 1995

The cytotoxicity-directed fractionation of MeOH extract of Terminalia chebula fruits led to the isolation of three hydrolyzed tannins and a related compound, gallic acid(1), $1,2,3,4,6-penta-O-galloy-{\beta}-_D/-glucopyranose(II)$,. chebulagic acid (III) and chebulinic acid(IV), as active principles. They were shoen to exhibit moderate cytotoxicity against cultured human tumor cell lines including A-549, SK-OV-3, Sk-MEL-2, XF-498 and HCT-15 in vitro.