• Title/Summary/Keyword: SFP

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Identification and molecular characterization of a rabbit hemorrhagic disease virus variant (KV0801) isolated in Korea

  • Yang, Dong-Kun;Kim, Byoung-Han;Lee, Kyung-Woo;Kim, Ji-Yeon;Kim, Hee-Jin;Choi, Sung-Suk;Chun, Ji-Eun;Son, Seong-Wan
    • Korean Journal of Veterinary Research
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    • v.49 no.3
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    • pp.207-213
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    • 2009
  • Rabbit hemorrhagic disease (RHD) is caused by RHD virus (RHDV) and is one of the most fatal diseases of rabbits. Acute death of rabbits occurred in a farm located in the Gyeonggi province of South Korea. The virus was isolated and confirmed as RHDV based on reverse transcription polymerase chain reaction and hemagglutination assay (HA), and the isolate was designated as KV0801. The nucleotide sequence of the complete VP60 gene of KV0801 was determined and the corresponding amino acid sequence was deduced. Molecular analysis showed that the KV0801 isolate can be classified as a pandemic antigenic variant strain, RHDVa. The VP60 nucleotide sequence and deduced amino acid homology between KV0801 and other Korean isolate, RHF89, which was isolated in 1988, were 92.1 and 94.3%, respectively. The pathogenicity of the KV0801 isolate at an HA titer ranging from 16,384 to 0.16 HA units was evaluated in five-month-old SFP rabbits. The rabbits inoculated with KV0801 isolate containing more than 1.63 HA units died within six days of inoculation. These results suggest that a highly pathogenic RHDVa is circulating in the rabbit populations of Korea.

Isolation, Identification, and Characterization of Bacillus strains from the Traditional Korean Soybean-fermented Food, Chungkookjang

  • Joo, Myeong-Hoon;Hur, Sung-Ho;Han, Yong-Soo;Kim, Ji-Yeon
    • Journal of Applied Biological Chemistry
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    • v.50 no.4
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    • pp.202-210
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    • 2007
  • A total of 45 bacterial strains were isolated from the traditional Korean soybean-fermented food, Chungkookjang. Among these strains, seven strains were selected and identified based on morphological, physiological, and biochemical characteristics, as well as phylogenetic analysis using 16S rDNA sequences. All strains were Gram-positive, aerobic, motile, oxidase-positive, rod-shaped, and endospore-forming bacteria, and produced extracellular enzymes such as amylase, cellulase, lipase, protease, and xylanase. The isolates were grown in the presence of 0-11% (w/v) NaCl. Growth was optimal at pH 6-9 and at temperatures of $30-45^{\circ}C$. According to VITEK automicrobic system tests and supplementary tests, the isolates were similar to several species of the genus Bacillus. The phylogenetic analysis of seven bacterial strains based on comparisons of 16S rDNA sequences, revealed that the strains were closely related to Bacillus species. The identification of strains that produced surfactin was also carried out, based on PCR screening of the sfp gene. Among the seven isolated strains, six yielded a surfactin-positive result with PCR.

Full System Chemical Decontamination Concept for Kori Unit 1 Decommissioning (고리1호기 해체시 전계통 화학제염 운전개념)

  • Lee, Doo Ho;Kwon, Hyuk Chul;Kim, Deok Ki
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.14 no.3
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    • pp.289-295
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    • 2016
  • Kori Unit 1, the first PWR (Pressurized Water Reactor) plant in Korea, began its commercial operation in 1978 and will permanently shut down on June 18, 2017. After moving the spent fuels to SFP (Spent Fuel Pool) system, Kori Unit 1 will perform a full system chemical decontamination to reduce radiation levels inside the various plant systems. This paper will describe the operation concept of the full system chemical decontamination for Kori Unit 1 based on experiences overseas.

Impact of PSI-KIT Nitriding model on hypothetical Spent Fuel Pool accident simulation

  • Mateusz Malicki;Terttaliisa Lind
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2504-2515
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    • 2023
  • In past years the Paul Scherrer Institute (PSI, Switzerland) and the Karlsruhe Institue of Technology (KIT, Germany)) collaborated to develop a model to account for the active role of nitrogen in the air oxidation of a Zircalloy cladding. The "PSI-KIT Nitriding Model for Zirconium based Fuel Cladding" model was implemented at PSI into PSI-MELCOR 1.8.6. In order to make a preliminary evaluation of the effect of the new model on the evolution of full-scale spent fuel pool accidents, one spent fuel pool event was analyzed using the PSI research version of PSI-MELCOR 1.8.6, which includes the nitriding model. To adapt an existing input deck for the calculations, a sensitivity study was conducted to find an optimal nodalization for the analyses. The nitriding model results were compared to those calculated with the MELCOR 1.8.6-PSI without the new nitriding model. The results demonstrate the effect of the nitriding reactions in spent fuel pool accident progression. Moreover, they confirm the impact of ZrN formation during cladding oxidation in air when the oxidation reactions lead to oxygen starvation inside the fuel assemblies. The nitriding reaction led to higher chemical heat generation during the accident and to an earlier failure of the cladding than when the effect of nitrogen reactions was not considered. It should be noted that the nitriding model, as implemented in the PSI version of MELCOR 1.8.6 has not yet been conclusively validated. Thereby the results presented in this paper should be treated as a preliminary demonstration of the capabilities of the model.

Characteristics and Lytic Activity of Phage-Derived Peptidoglycan Hydrolase, LysSAP8, as a Potent Alternative Biocontrol Agent for Staphylococcus aureus

  • Yu, Jun-Hyeok;Lim, Jeong-A;Chang, Hyun-Joo;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.29 no.12
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    • pp.1916-1924
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    • 2019
  • Outbreaks of staphylococcal food poisoning (SFP) causing serious human diseases and economic losses have been reported globally. Furthermore, the spread of Staphylococcus aureus with increased resistance to multiple antimicrobial agents has become a major concern in the food industries and medicine. Here, we isolated an endolysin LysSAP8, as one of the peptidoglycan hydrolases, derived from the bacteriophage SAP8 infecting S. aureus. This endolysin was tagged with a 6×His at the C-terminal of the target protein and purified using affinity chromatography. LysSAP8 demonstrated lytic activity against a broad spectrum of bacteria, which included a majority of the staphylococcal strains tested in this study as well as the methicillin-resistant S. aureus (MRSA); however, no such activity was observed against other gram-positive or gram-negative bacteria. Additionally, LysSAP8 could maintain bactericidal activity until 0.1 nM working concentration and after heat treatment at 37℃ for 30 min. The ability of LysSAP8 to lyse cells under varying conditions of temperature (4-43℃), pH (3-9), and NaCl concentrations (0-1,000 mM), and divalent metal ions (Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Hg2+, and Zn2+) was examined. At the optimized condition, LysSAP8 could disrupt approximately 3.46 log CFU/ml of the planktonic cells in their exponential phase of growth within 30 min. In this study, we have suggested that LysSAP8 could be a potent alternative as a biocontrol agent that can be used to combat MRSA.

Fabrication of the Integrated Triplexer Using Micro Block Stacking Method (Micro-block Stacking 방법으로 제작한 집적형 Triplexer 제작 및 특성 측정)

  • Yoon, Hyun-Jae;Kim, Jin-Won
    • Korean Journal of Optics and Photonics
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    • v.23 no.5
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    • pp.217-221
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    • 2012
  • In this paper, we have fabricated by means of the "Micro-Block Stacking (MBS)" method the 8 pin mini DIL integrated Triplexer, which can transmit CATV and voice/data at the same time in a single fiber. Our MBS technique is a novel scheme of compact optical module packaging which secures precision positioning of the components on the optical beam path by prefixed stacks of ceramic blocks. The subassembly in which a laser diode, two receiver photodiodes, two WDM filters, and four micro lenses are integrated is only $5.40mm{\times}2.15mm{\times}1.05mm$ in size. As the Triplexer is aligned to the single mode fiber, the transmitter power of -14.5 dBm and the receiver sensitivities of 0.83 A/W, 0.73 A/W for 1550 nm, 1490 nm, respectively are obtained.

Developing a STEP-NC Prototype based on ISO 14649 Paradigm (ISO14649 패러다임에 입각한 STEP-NC 프로토타입 시스템 개발)

  • Seo, Seok-Hwan;Jo, Jeong-Hun;Jeong, Dae-Hyeok;Lee, Byeong-Eon;Cheon, Sang-Uk
    • Journal of the Korean Society for Precision Engineering
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    • v.19 no.7
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    • pp.171-179
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    • 2002
  • STEP-NC is the next generation CNC controller taking STEP-based data model as the interface scheme (or language) between CAM and CNC, and carrying out various intelligent functions. At the moment, efforts are being made worldwide to establish international standard for the new interface scheme formalized as ISO14649. As the new language is being established, increasing attention is being paid to the development of the new CNC. Korea STEP-NC is an integrated STEP-NC system taking ISO 14649 as an input, and carrying out various intelligent functions. It is composed of 5 modules: 1) Shop Floor Programming System (PosSFP), 2) Tool Path Generator (PosTPG), 3) Tool Path Viewer (PosTPV), 4) Man Machine Interface (PosMMI), and 5) CNC Kernel (PosCNC). Distinguished from other prototypes (of Europe and USA), the Korea STEP-NC is top-down designed, and bottom-up implemented comprehensively incorporating all the crucial components for realizing the full benefit of STEP-NC paradigm, without using any existing commercial CAD/CAM systems and CNC kernels. The Korea STEP-NC prototype was successfully demonstrated and evaluated in the ISO conventions Together with prototypes of Europe and USA, Korea STEP-NC will be used as a reference system fur the Triangular Conformance Test to be jointly carried out by ISO TC184 SC1, SC4, and IMS Project.

Effects of processing conditions on water vapor permeability and solubility of Alaska Pollack meal protein isolate film (가공조건이 명태어분단백질 필름의 수증기 투과도와 용해도에 미치는 영향)

  • YOU Byeong-Jin;SHIM Jae-Man
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.413-417
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    • 2000
  • The effects of film processing conditions on the water vapor permeability (WVP), the solubility of film's protein (SFP) and the soluble film's meterials amount (SFMA) were investigated to establish the conditions for preparing biodegradable and edible Alaska Pollack meal protein isolate (APMPI) film. ms of film were decreased with increasing plasticizer concentration but those were decreased with decrement of APMPI's pH values. SEPs were decreased with increasing APMPI's pH and plasticizer concentration. SFMAS were also strongly affected by plasticizer concentration and APMPI's pH. In the case of adding different plasticizers, WVP was increased in order as follows: glycerol, polyethylene glycol and sorbitol but SFMA showed inverse order.

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Effect of Urethane Modification on the Anti-Bullet Property of Dyneema/vinylester Composites (우레탄 수지 첨가에 의한 다이니마/비닐에스터 복합재료의 방탄효과 향상 연구)

  • Yoon, T.H.;Cha, Y.M.;Yuck, J.I.;Paik, J.G.;Oh, Y.J.;Kim, H.J.
    • Composites Research
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    • v.24 no.6
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    • pp.7-11
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    • 2011
  • Polyurethane oligomers (PUOs) such as UA8297, UP127 and EB8200 were utilized to enhance the anti-bullet property of Dyneema$^{(R)}$/vinylester composites. First, prepregs of PUO and vinylester (XSR10) were prepared via spray coating on Dyneema$^{(R)}$ fabric at 21 % resin content (by volume). In addition, spray coating and film lamination were also carried out with a mixture of XSR10/PUO for selected PUOs. Next, the prepregs were dried at RT for 1-2 h and then at $100^{\circ}C$ for 30 min to remove the solvent and to provide partial cure when necessary. The prepregs were stacked in 24 layers and cured at $120^{\circ}C$ for 5 min under the contact pressure and for additional 25 min at 150 $kg/cm^2$. Finally, the anti-bullet properties of composite samples were evaluated by measuring $V_{50}$ with simulated fragment projectile (SFP, 17 gr). The results showed a 6.5 and 9.0 % increase of $V_{50}$ with UP127 and EB8200, respectively.

Mix design and early-age mechanical properties of ultra-high performance concrete

  • Tang, Chao-Wei
    • Advances in concrete construction
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    • v.11 no.4
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    • pp.335-345
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    • 2021
  • It is known from the literature that there are relatively few studies on the engineering properties of ultra-high performance concrete (UHPC) in early age. In fact, in order to ensure the safety of UHPC during construction and sufficient durability and long-term performance, it is necessary to explore the early behavior of UHPC. The test parameters (test control factors) investigated included the percentage of cement replaced by silica fume (SF), the percentage of cement replaced by ultra-fine silica powder (SFP), the amount of steel fiber (volume percent), and the amount of polypropylene fiber (volume percentage). The engineering properties of UHPC in the fresh mixing stage and at the age of 7 days were investigated. These properties include freshly mixed properties (slump, slump flow, and unit weight) and hardened mechanical properties (compressive strength, elastic modulus, flexural strength, and splitting tensile strength). Moreover, the effects of the experimental factors on the performance of the tested UHPC were evaluated by range analysis and variance analysis. The experiment results showed that the compressive strength of the C8 mix at the age of 7 days was highest of 111.5 MPa, and the compressive strength of the C1 mix at the age of 28 days was the highest of 128.1 MPa. In addition, the 28-day compressive strength in each experimental group increased by 13%-34% compared to the 7-day compressive strength. In terms of hardened mechanical properties, the performance of each experimental group was superior to that of the control group (without fiber and without additional binder materials), with considerable improvement, and the experimental group did not produce explosive or brittle damage after the test. Further, the flexural test process found that all test specimens exhibited deflection-hardening behavior, resulting in continued to increase carrying capacity after the first crack.