• Journal of Convergence for Information Technology
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• v.9 no.1
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• pp.142-150
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• 2019

This study is a file management security system that encrypts and decrypts computer and cloud data by using Bluetooth based cryptographic module. It is a necessary solution in terms of abuse of personal information and protection of social and national information. We developed H/W and S/W for SFMS(: Safe File Management Security) related Bluetooth module in cloud environment and implemented firmware development, encryption key generation and issuance, client program for system mobile and key management system. In the terminal internal encryption and decryption, SFMS was developed to ensure high security that the hacking itself is not possible because key values exist separately for each file.

• The Journal of the Korea institute of electronic communication sciences
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• v.14 no.1
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• pp.243-250
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• 2019

In this paper, we develop a Secure File Management Security(: SFMS) based on media in a cloud environment to encrypt and decrypt cloud data on a computer using a Bluetooth - based cryptographic module. The Bluetooth cipher module makes it easy to browse files stored in the cloud, but it is never possible to browse without a module. It is a solution that fundamentally blocks the problems such as hacking and leakage of personal data that have recently become an issue.

• Journal of the Korea Society of Computer and Information
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• v.16 no.4
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• pp.189-196
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• 2011

The main issues of the researches are monitoring environment such as weather or temperature variation and natural accident, and sensor gateways which have mobile device, applications for mobile health care. In this paper, we propose the SFMS(Smart Factory Management System) that can effectively monitor and manage a green smart factory area based on M2M service and smart phone with android OS platform. The proposed system is performed based on the TinyOS-based IEEE 802.15.4 protocol stack. To validate system functionality, we built sensor network environments where were equipped with four application sensors such as Temp/Hum, PIR, door, and camera sensor. We also built and tested the SFMS system to provide a novel model for event detection systems with smart phone.

• Bulletin of the Korean Chemical Society
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• v.32 no.6
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• pp.2105-2108
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• 2011

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.319-324
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• 2006

Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology industry. For this reason, many research groups, including our laboratory, have been trying to develop serum-free media (SFM) or serum-supplemented media (SSM) for special or multi-purpose cell lines. The Chinese hamster ovary (CHO) cell, for example, is frequently used to produce proteins and is especially valuable in the large-scale production of pharmaceutically important proteins, yet information about its genome is lacking. Also, SFMs have only been evaluated by comparing growth patterns for cells grown in SFMs with those grown in SSM or by measuring the titer of the target protein obtained from cells grown in each type of medium. These are not reliable methods of obtaining the type of information needed to determine whether an SFM should be replaced with an SSM. We carried out a cDNA microarray analysis to evaluate MED-3, an SFM developed in our laboratory, as a CHO culture medium When CHO cells were cultured in MED-3 instead of an SSM, several genes associated with cell growth were down-regulated, although this change diminished over time. We found that the insulin-like growth factor (IGF) gene was representative of the proteins that were down-regulated in cells cultured in MED-3. When several key supplements - including insulin, transferrin, ethanolamine, and selenium - were removed from MED-3, the IGF expression was consistently down- regulated and cell growth decreased proportionately. Based on these results, we concluded that when an SFM is used as a culture medium, it is important to supplement it with substances that can help the cells maintain a high level of IGF expression. The data presented in this study, therefore, might provide useful information for the design and development of SFM or SSM, as well as for the design of genome-based studies of CHO cells to determine how they can be used optimally for protein production in pharmaceutical and biomedical research.

• Journal of Korean Society for Geospatial Information Science
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• v.12 no.3 s.30
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• pp.3-12
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• 2004

Facilities Management(FM) System is widely spread GIS applications introduced in local governments. Over the last few years many local governments in Korea have developed facilities management systems, especially for underground facilities(drinking water and sewerage) and road management systems. The concept of Standardized Facilities Management Software(SFMS) was developed to minimize the duplication of efforts in local governments. Recently local governments try to change their direction to Urban Information Systems(UIS) which can comprehensively manage their information project carried out by each sector. Therefore this study suggests a method for introducing a Standardized Facilities Management Software to help constructing urban information system. This method is expected to prevent double investment, reduce project time, minimize the risk of project failure, and activate information projects.

• Journal of Life Science
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• v.22 no.3
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• pp.373-379
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• 2012

This study was performed to evaluate the antioxidant and antimicrobiological effects of supercritical fluid extracts (SFEs) and Marc methanol extracts (SFMs) from Cinnamomum verum. Reducing effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and ABTS radical scavenging were investigated. SFM exhibited higher antioxidant activities in DPPH and ABTS radical scavenging assay. Measurements of the antimicrobial activity were used for Gram-positive bacteria (four strains) and Gram-negative bacteria (four strains). The antimicrobial activities of the SFE and SFM against gram-positive and gram-negative bacteria revealed that SFE had a higher inhibition zone than SFM. Cinnamaldehyde, the active compound of C. verum, had a higher content in SFM (35% at 300 bar, $30^{\circ}C$) than methanol extracts from C. verum (0.5%). These results indicate that not only SFE oil, but also SFM, could be a good source for the food industry.

• KSBB Journal
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• v.21 no.2
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• pp.110-117
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• 2006

During routine maintenance, animal cell lines are commonly cryopreserved in growth medium containing serum with 10% DMSO. But, in case of bioprocess under the serum-free conditions, including cultivation of cell lines and producing of pharmaceuticals, the cryopreservation should be executed without serum to prevent a cross-contamination. This experiments were performed to investigate the effects of the serum-free cryopreservation on the CHO cells. To improve the survival rates of the cryopreserved CHO cells in serum-free condition, first, the effects of permeable and non-permeable additives for substitute serum on cell viability were investigated. The combination of 10% DMSO and 0.03 M raffinose in MEM-${\alpha}$ without serum indicated 76% of cell viability. However, it did not reach the survival rates(more than 95%) of the conventional cryopreservation. In the second, to evaluate the cryopreservative ability of the serum-free medium(SFM) we compared viability of the CHO cells cryopreserved in the SFMs(Sigma C5467, C4726, and C1707, JBI SF486 and PF486), the cryoprotectant(Genenmed CAN-1000) and the MEM-${\alpha}$ with serum. All solution contained 10% DMSO. As a result of the comparison, cryopreserved cells in the SFMs showed over 95% of viability and appeared predominant viability better than cryoprotectant CAN-1000. Finally, we assessed the stability of the CHO cells in the long-term cryopreservation(LTC) using SFM. Every three months, the cryopreserved CHO cells were thawed to estimate the cell viability and the recovery rates. Then, real-time RT-PCR analyzed the inserted CHO DHFR gene. All results for the LTC appeared the same stability as the serum containing cryopreservation. In the conclusion, it could be seen that the LTC in the SFM can substitute for serum using methods in the bioprocess proceeded by CHO cells for more than 18 months.