• 분석과학
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• 제30권2호
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• pp.57-67
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• 2017

This study was conducted to develop an analytical technique for determination of chlorite and chlorate concentrations in fresh-cut food and dried fish products by an ion chromatography/conductivity detection method using a hydroxide mobile phase. Deionized water was added to homogenized samples, which were then extracted by ultrasound extraction and centrifuged at high speed (8,500 rpm). Subsequently, a Sep-Pak tC18 cartridge was used to purify the supernatant. Chlorite and chlorate ions were separated using 20 mM KOH solution as the mobile phase and Dionex IonPac AS27 column as the stationary phase. Ethylenediamine was used as sample preservative and dibromoacetate was added to adjust for the disparity in extraction efficiencies between the food samples. The method detection limit) for chlorite and chlorate were estimated to be 0.2 mg/kg and 0.1 mg/kg, respectively, and the coefficient of determination ($r^2$) that denotes the linearity of their calibration curves were correspondingly measured to be 0.9973 and 0.9987. The recovery rate for each ion was 92.1 % and 96.3 %, with relative standard deviations of 7.47 % and 6.18 %, respectively. Although neither chlorite nor chlorate was detected in the food samples, the analytical technique developed in this study may potentially be used in the analysis of disinfected food products.

• Archives of Pharmacal Research
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• 제28권4호
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• pp.463-468
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• 2005

The purpose of the present study was to develop a standard protocol for imidapril hydrochloride bioequivalence testing. For this reason, a specific LC-MS method was developed and validated for the determination of imidapril in human plasma. A solid-phase extraction cartridge, $Sep-pak^{R}$ C18, was used to extract imidapril and ramipril (an internal standard) from deproteinized plasma. The compounds were separated using a XTerra $MS^{R}$?C18 column ($3.5 {\mu}m, 2.1\times150 mm$) and $acetonitrile-0.1\%$ formic acid (67:33, v/v) adjusted to pH 2.4 by 2 mmol/L ammonium formic acid, as mobile phase at 0.3 mL/min. Imidapril was detected as m/z 406 at a retention time of ca. 2.3 min, and ramipril as m/z 417 at ca. 3.6 min. The described method showed acceptable specificity, linearity from 0.5 to 100 ng/mL, precision (expressed as a relative standard deviation of less than $15\%$), accuracy, and stability. The plasma concentration-versus-time curves of eight healthy male volunteers administered a single dose of imidapril (10 mg), gave an $AUC_{12hr}$ of imidapril of $121.48\pm35.81 ng mL^{-1} h$, and $C_{max} and T_{max}$ values of $32.59\pm9.76 ng/mL and 1.75\pm0.27 h$. The developed method should be useful for the determination of imidapril in plasma with sufficient sensitivity and specificity in bioequivalence study.

• 한국식품영양과학회지
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• 제21권2호
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• pp.187-194
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• 1992

Rhizopus oryzae CJ-2114의 polygalacturonase 생성을 위한 최적조건은 수분이 60% 함유된 밀기울 배지에 1% albumin, 0.2% (NH$_4$)$_2$C$_2$O$_4$, 1% sorbitol을 첨가하여 96시간 배양시 최대활성을 나타내었으며, Sep-hadex G-75 및 G-150을 사용한 gel filtration과 DEAE-cellulose에 의한 이온교환 크로마토그라피를 통하여 이 효소를 11.13배 정제할 수 있었고, 수율은 40.3% 였다. 정제효소는 polyacrylamide gel 전기 영동에 의하여 단일밴드로 확인되었으며 분자량은 SDS-polyacryl-amide 전기영동에 의하여 47,000정도로 측정되었다. 효소의 결정구조는 표면이 거친 기둥모양을 형성하고 있었으며 아미노산 조상은 17종류로써 glutamic acid 함량이 198.74mg/g enzyme로 가장 많았다.

• 지역사회간호학회지
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• 제8권2호
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• pp.225-236
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• 1997

This study was conducted to investigate the elderly in urban and rural ares. The subjects were selected in a convenient sampling and the total number was 189(Urban : 95, Rur al : 94). The data were collected by one to one interviews in the period of Sep. 1-30, 1995(Koje) and March 15-28, 1997 (Taejon). The study tools for this study were 1) ADL and IADL 2) Self rating scores for health status. The data were analyzed by percentage, T-test, ANOVA, $X^2$ Test, Pearson correlation coefficiency by SPSS pc WIN. 7.0 program. The results were as follows: 1. The self rating score for health status of the elderly in urban area was lower than that of the rural when compared in the same age group. 2. In the comparison of ADL scores between the elderly in urban and rural areas, there was no statistically significant difference. The IADL score of the rural elderly were higher than that of the urban elderly and there was a statistically significant difference. 3. In the comparison of ADL & IADL scores according to the self rating score for health status, there was a statistically significant difference among health status levels.

• 천문학회보
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• 제45권1호
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• pp.46.1-46.1
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• 2020

DEEP-South (DEep Ecliptic Patrol of the Southern Sky) team will start the 2nd phase of KMTNet observation in Oct 2020. The DEEP-South observation mainly consists of three survey modes: (1) Activity survey (AS) that aims at finding active phenomena of small Solar System bodies. (2) Light curve survey (LS) targets to discover and characterize light variations of asteroids. And (3) Deep drilling survey (DS) focuses on the objects beyond the orbit of Jupiter (Centaurus and trans-Neptunian objects) as well as near Earth asteroids. For asteroid family (AF) studies and target of opportunity (TO) observations for urgent photometric follow-up, targeted mode will also be used. DEEP-South team is awarded 7.0% of the telescope time at each site every year from Oct 2020 to Sep 2023 in the 2nd phase of KMTNet operation which corresponds to about 75 full nights a year for the network. In this presentation, we will introduce our survey strategy and observation plan.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권2호
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• pp.69-75
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• 2009

Daba ecorace of Antheraea mylitta Drury (Lepidoptera: Saturniidae), the semi domesticated Indian tropical tasar silkworm being reared outdoor; the egg and silk yields are dependent of genotype environment interaction. The insufficient maintenance and multiplication of its P4 seed stock need a coherent as well as scientific strategy to safeguard breed potential, being commercially applied ecorace. The sort-out lines of P4 stock studied over five generations highlighting on commercial trait up gradation suits for a tropical crop season, revealed enhanced performance. The line with high pupal parents (T2) shown improved fecundity (12.9%) and the line with high shell parents (T3) recorded higher shell weight (40.0%) and silk ratio (24.1%). While, the line of high pupal female and high shell male (T4) reveal enhancement in fecundity (9.0%), egg hatching (14.1%), shell weight (50.0%), silk ratio (35.2%) and absolute silk yield (52.0%) indicating the need and role of varied basic seed stock lines. The approach could improve economically vital egg fecundity and cocoon shell weights besides balancing them in same line for commercial operation. The progressive show of lines (T1 to T4) along successive generations (G1 to G5), in spite of passing through seed crop (Jul-Aug) and commercial crop (Sep-Nov) seasons emphasize their compatibility. The study infers that the strategic plan of combining preferred parental phenotypes, methodical selection for desired commercial trait(s) through generations with best possible genotype environment interaction has enriched P4 stock with elevation in needy trait(s) besides assuring choice of suitable lines for seasons and regions and timely replenishment of basic seed of Daba ecorace.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1519-1519
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• 2001

The aim of this work was to test the feasibility of NIRS in analysing textural characteristics of “Pasta Filata” cheese during the shelf-life. For this purpose, 128 samples of “Pasta Filata” cheese, subdivided into two sets on the basis of the wax used to avoid mechanical damages (paraffin, biodegradable wax), were analysed by using an InfraAlyzer 500 (Bran+Luebbe). Analyses were performed at room temperature. Samples were cut into small cylinders (D=3.2 cm, height = 1 cm), in agreement with literature information. Data were processed by using Sesame Software (Bran+Luebbe). Samples were analysed, during the shelf-life, at 90 and 120 days. In parallel, textural characteristics were detected carrying out a compression method by using an Universal Testing Machine Instron model 4301 (Instron Corporation, Canton, Massachusetts). As compression probe was used a cylinder (D = 5.8 cm, height = 3.7 cm) and a speed rate of 20mm/min was applied. The load at 20 mm of compression was recorded on sample cylinders of 1.7 cm (D) by 2 cm (height). Qualitative analysis of full spectra showed the possibility to gather samples on the basis of the days of shelf-life. The textural characteristics of cheese during the shelf-life was evaluated by comparing NIRS data with rheological results. The best correlation was obtained applying MLR to the first derivative of normalized absorbance values at seven wavelengths. Load values were plotted against the NIR prediction values based on first derivatives. NIRS proved to be an useful tool in classifying samples on the basis of the shelf-life period as well as in predicting their textural characteristics ($R^2$= 0.916, SEC = 0.192, SEP = 0.248, SEV = 0.345).

• 환경위생공학
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• 제10권2호
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• pp.36-45
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• 1995

Physicochemical environments and phytoplankton community in the nakdong river middle basin were investigated during a period of 1 year from Nov. 1993 to Oct. 1994. The ranges of Physicochemical environment factors in the nakdong river middle basin were $3.6~27.4{\circ}C$ for temperature, 7.1~9.3 for pH, $7.1~12.7mg/{\ell}$for DO, $0.7~2.1mg/{\ell}$ for BOD, $31~52mg/{\ell}$ for alkalinity, $2.101~3.3mg/{\ell}$ for T-N, $0.052~0.099mg/{\ell}$ for T-p, $5.4~92.3mg/m^{3}$ for chlorophyll-a. The number of Phytoplankton was lowest in Jan. that is, 244ce11s/ml and that was highest in Sep. 1201 cells/ml. The increase of dominant genera were correlated with the total nomber of phytoplanktons. Bacillariaceae, chlorophyceae, cyanophyceae, and euglenophlrceae were to 74.5%, 20%, 3.5%, and 2.2% respectively. Bacillariaceae were abundant during all the year round except for Jun, Jul, Aug. Chlorophyceae were plentiful in Jun, Jul, Aug. Tase-and odor-causing algae and filter clogging algae were 72% in Nov. and 93.5% in Jun. They were abundant all the year round except for winter, so they influenced on the treatment of tap water system. They belong to the general asterionella, cyclotella, melosira, nitzschia of bacillariaceae and actinastrum, pediastrum of chlorophyceae. Dominace index was low in winter and high in umber. Diversity index and equitability index were low in summer and high in winter. Therefore, the structure of phytoplankton community of the nakdong river middle basin was different form season to season.

• 한국작물학회지
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• 제53권spc호
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• pp.108-114
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• 2008

녹차 품질평가의 한 요인이 되는 색도 평가 시 기존 평가 방법인 육안평가 혹은 색차 분석에 의존하고 있는 현행 분석방법을 신속, 간편하며 재현성이 높고, 녹차 품질관련 기타 성분과 동시분석이 가능한 녹차 색차 분석용 NIRS 검량식을 작성한 결과를 요약하면 다음과 같다. 1. 공시된 녹차 시료를 대상으로 색차계를 이용하여 색도 값(L, a, b)을 조사한 결과 검량식 작성용 시료는 L값이 평균 53.37($48.52{\sim}57.72$), a값이 평균 -7.55($-10.02{\sim}-4.63$), b 값이 평균 18.07($14.00{\sim}22.02$)을 나타내었고, 작성 검량식의 평가용으로 이용된 예견치 분석용 시료와 거의 동일한 범위를 나타내었다. 2. 녹차의 색차 분석용 NIRS 검량식을 검토한 결과 색차 중 명도에 해당하는 L 값은 원시 스펙트럼에 2차 미분(2nd derivative, 8 nm gap, 6 points smoothing, 1 point second smoothing)을 수행한 조건에서 $R^2$ = 0.936으로 가장 우수한 양상을 나타내었고, 적색에 해당되는 색차 a값과 황색에 해당하는 b값은 1차 미분(1st derivative, 4 nm gap, 4 points smoothing, 1 point second smoothing)조건에서 $R^2$가 각각 0.991 및 0.958로 가장 우수한 결과를 나타내었다. 3. 최적의 녹차 색차 분석용으로 작성된 각각의 NIRS 검량식을 미지시료에 적용하여 정확성을 평가한 결과 색도값 L, a 및 b의 결정계수는 각각 0.905, 0.986 및 0.931로 매우 높은 상관을 보였으며, 이들 검량식은 향후 NIRS를 이용한 녹차 관련 연구 및 녹차 산업현장에서 품질관리를 위한 효율적 분석방법으로 활용이 가능할 것으로 판단된다.

• 한국학교보건학회지
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• 제13권2호
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• pp.319-329
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• 2000

This study suggestes that the improvement of oral care through on investigation of the effects of oral care on children by parents. This investigation was done on Sep. 29 1999 through Oct. 8 examining 376 of elementary, 1grade, 2grade, 3grade students in Kyunggi-Do. The content of the questions was analzed with a t-test and a one-way ANOVA; the relation to the oral care on children by parents with oral care behavior was analyzed by a Pearson's Correlation Coefficient. The results are as follow; 1) Statistically women were higher(F=-2.675, p<0.01). The average of the oral care of children was 2.34, man averaged 2.29, woman are raged 2.32. Statistically women were also higher(F=4.682, p<0.052). 2) The more educatior the mother had higher, the higher the grade. Statistically there was a difference(F=6.756, p<0.01. The oral care of children above Uni graduate(M=2.38), high (M=2:23), middle graduate(M=2.Z3). Statistically, the higher the academic degree, the more optimistic(F=4.114, p<0.05). 3) In the oral care on children by parents, when the conversation with the family was often 2.17, there was little difference(F=7.343, p<0.01). In the oral care of children was 2.45, there was little difference(F=7.343. p<0.01). As a result, the conversation with family affects the behavior. 4) In the oral care of children, if the food that they like was fruits, vegetables, and fresh food, the average was 2.37, very high. There was little difference in other cases(F=-3.179, p<0.05). We can conclude that fresh food affects the oral care of children. 5) In the oral care on children by parents, when the course of knowledge on the oral care was viewed by a grandfather(M=2.02), grandmother(M=2.05), father(M=2.14), mother(M=2.08). There was little difference(F=2.666, p<0.05). 6) In oral care, if the level of income was viewed high level(M=2.42), middle(M=2.34), 10w(M=1.88), there was a little difference(F=3.300, p<0.05). 7) The relation of the oral care on children by parents and the oral care was normal. In other words, the more management by parents, the more management by children.(p<0.01) Therefore in this study, the oral care on children by parents effect the oral care behavior of children. To improve the mouth health of children, the parents's interest in their children must be constant. Also, it is needed to develope an education program where parents and children can be educated.