• Title/Summary/Keyword: SC1

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Development of a Multiplex PCR for Simultaneous Detection of Blueberry Red Ringspot Virus and Blueberry Scorch Virus Including an Internal Control

  • Hae Min Lee;Eun Gyeong Song;Ki Hyun Ryu
    • Research in Plant Disease
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    • v.29 no.1
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    • pp.94-99
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    • 2023
  • Blueberry red ringspot virus (BRRSV) and blueberry scorch virus (BlScV) are included in the quarantine virus list managed by the Korean Animal and Plant Quarantine Agency. A multiplex polymerase chain reaction (PCR) assay with an internal control was developed for the simultaneous detection of both viruses. The specific primers used here were designed based on the highly conserved regions of the genomic sequences of each virus, obtained from the National Center for Biotechnology Information nucleotide databases. The primers were designed to amplify a partial sequence within coat protein (CP) for detecting BRRSV and a partial sequence within the CP-16 kDa for detecting BlScV. 18S ribosomal RNA (rRNA) was used as internal control, and the primer set used in a previous study was modified in this study for detecting 18S rRNA. Each conventional PCR using the BRRSV, BlScV, and 18S rRNA primers exhibited a sensitivity of approximately 1 fg plasmid DNA. The multiplex PCR assay using the BRRSV, BlScV, and 18S rRNA primers was effective in simultaneously detecting the two viruses and 18S rRNA with a sensitivity of 1 fg plasmid DNA, similar to that of conventional PCR assays. The multiplex PCR assay developed in this study was performed using 14 blueberry cultivars grown in South Korea. BRRSV and BlScV were not detected, but 18S rRNA was all detected in all the plants tested. Therefore, our optimized multiplex PCR assay could simultaneously detect the two viruses and 18S rRNA in field samples collected from South Korea in a time-efficient manner. This approach could be valuable in crop protection and plant quarantine management.

A study on Secure Communication in Hyper-Chaos with SC-CNN using Embedding Method

  • Bae, Young-Chul;Kim, Ju-Wan;Song, Hag-Hyun;Kim, Yoon-Ho
    • Journal of information and communication convergence engineering
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    • v.1 no.4
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    • pp.223-228
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    • 2003
  • In this paper, we introduce a hyper-chaos secure communication method using hyper-chaos circuit onsist of State-Controlled Cellular Neural Network SC-CNN). We make a hyper-chaos circuit using SC-CNN with the n-double scroll or Chua's oscillator. A hyper-chaos circuit is created by applying identical n-double scroll or non-identical n-double scroll and Chua's oscillator with weak coupled method to each cell. Hyper-chaos ynchronization was achieved using GS (Generalized Synchronization) method between the transmitter and receiver about each state variable in the SC-CNN. In order to secure communication, we have synthesizing the desired information with a hyper-chaos circuit by adding the information signal to the hyper-chaos signal using the SC-CNN in the transmitter. And then, transmitting the synthesized signal to the ideal channel, we confirm secure communication by separating the information signal and the hyper-chaos signal in the receiver.

ON SPACES IN WHICH COMPACT-LIKE SETS ARE CLOSED, AND RELATED SPACES

  • Hong, Woo-Chorl
    • Communications of the Korean Mathematical Society
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    • v.22 no.2
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    • pp.297-303
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    • 2007
  • In this paper, we study on C-closed spaces, SC-closed spaces and related spaces. We show that a sequentially compact SC-closed space is sequential and as corollaries obtain that a sequentially compact space with unique sequential limits is sequential if and only if it is C-closed [7, 1.19 Proposition] and every sequentially compact SC-closed space is C-closed. We also show that a countably compact WAP and C-closed space is sequential and obtain that a countably compact (or compact or sequentially compact) WAP-space with unique sequential limits is sequential if and only if it is C-closed as a corollary. Finally we prove that a weakly discretely generated AP-space is C-closed. We then obtain that every countably compact (or compact or sequentially compact) weakly discretely generated AP-space is $Fr\acute{e}chet$-Urysohn with unique sequential limits, for weakly discretely generated AP-spaces, unique sequential limits ${\equiv}KC{\equiv}C-closed{\equiv}SC-closed$, and every continuous surjective function from a countably compact (or compact or sequentially compact) space onto a weakly discretely generated AP-space is closed as corollaries.

Assessment of Solubility, Heavy Metals and Microbial Safety in Differently- Treated Muscle Tissues of Mackerel Scomber japonicus

  • Asaduzzaman, A.K.M.;Lee, Won-Kyoung;Chun, Byung-Soo
    • Fisheries and Aquatic Sciences
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    • v.17 no.1
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    • pp.59-65
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    • 2014
  • We measured the reductions in size and solubility of mackerel muscle that was freeze-dried, deoiled by supercritical carbon dioxide (SC-$CO_2$), or roasted. The percent size reduction and solubility were high in SC-$CO_2$-treated muscle compared with freeze-dried and roasted muscle. We used oil-free residues to test for heavy metals and determine microbial safety. The SC-$CO_2$, hexane, and ethanol were used to separate oil from muscle. The concentrations of cadmium (Cd) in all treated muscles were less than the values reported in the literature, as were the concentrations of lead in SC-$CO_2$- and hexane- treated muscle. In contrast, concentrations of arsenic and mercury in muscles were greater than the reported values regardless of treatment. Zinc and iron, which are beneficial for health, were found in high levels after all treatments of muscle tissue. After 6 months of storage at different temperatures, SC-$CO_2$-and ethanol-treated muscle showed few bacterial colonies, and none were found after 4 months of storage at $-20^{\circ}C$. These results will be useful to food-processing industries for maintaining high-quality, safe mackerel muscle.

Prognostic Factors in Gastric Cancer Patients with Peritoneal Carcinomatosis

  • Kim, Hyun-Il;Ha, Tae-Kyung;Kwon, Sung-Joon
    • Journal of Gastric Cancer
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    • v.10 no.3
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    • pp.126-132
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    • 2010
  • Purpose: Peritoneal carcinomatosis (PC) has a dismal prognosis and is occasionally encountered during initial exploration in patients with gastric cancer. The clinicopathological characteristics and survival were analyzed in patients with gastric cancer and PC. Materials and Methods: Among 2,083 gastric cancer patients who received surgery at the department of surgery, Hanyang University Hospital from 1992 to 2009, 130 patients revealed PC. Ten patients who were lost during follow-up were excluded. The remaining 120 patients were divided into three groups according to the type of surgery. The degree of PC was classified into P1(to the adjacent peritoneum) and P2 (to the distant peritoneum). Various other clinicopathological factors were analyzed using univariate and multivariatec survival analyses. Results: Systemic chemotherapy (SC), type of surgery, lymph node dissection, degree of PC, and presence of ascites were significant prognostic factors. However, age, gender, resection of PC, and Borrmann type were not significant prognostic factors. In a multivariate analysis, SC and the degree of PC were independent prognostic factors. The survival benefit of SC was significant without reference to the type of surgery or degree of PC. Conclusions: A gastrectomy should be considered feasible in patients with gastric cancer and PC. The independent favorable prognostic factors were SC and a low degree of PC. SC improved the prognosis regardless of operation type and degree of PC.

Effect of Saururus chinensis BAILL Extract for Pharmacopuncture on the melanogenesis in B16F10 cells (삼백초 약침액이 B16F10 흑색종세포의 멜라닌 합성에 미치는 영향)

  • Kim, Soo-Kyung;Kim, Dae-Sung;Woo, Won-Hong;Mun, Yeun-Ja
    • Korean Journal of Acupuncture
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    • v.29 no.1
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    • pp.117-130
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    • 2012
  • Objectives : The purpose of this study was to investigate the melanogenesis inhibition effect of Saururus chinensis BAILL (SC) on in B16F10 melanoma cells. Methods : SC was fractionated ethanol extract by the hexane, ethyl acetate, butanol and water. We confirmed the inhibitory effect of tyrosinase activity and melanogenesis of all fraction samples. Results : Hexane fraction of Saururus chinensis BAILL (HSC), ethyl acetate of SC (ESC), and butanol of SC (BSC) were discovered to inhibit tysoinase activity and melanogenesis in the absence or presence of ${\alpha}$-MSH. However, water fraction of SC (WSC) did not affect tyrosinase activity and melanogenesis. In addition, all fractions did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 melanoma cell lines. Conclusions : These results suggest that HSC, ESC and BSC reduce pigmentation by indirectly regulating tyrosinase.

Removal of Post Etch/Ash Residue on an Aluminum Patterned Wafer Using Supercritical CO2 Mixtures with Co-solvents and Surfactants: the Removal of Post Etch/Ash Residue on an Aluminum Patterned Wafer

  • You, Seong-sik
    • Journal of the Semiconductor & Display Technology
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    • v.16 no.2
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    • pp.55-60
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    • 2017
  • The supercritical $CO_2$ (sc-$CO_2$) mixture and the sc-$CO_2$-based Photoresist(PR) stripping(SCPS) process were applied to the removal of the post etch/ash PR residue on aluminum patterned wafers and the results were observed by scanning of electron microscope(SEM). In the case of MDII wafers, the carbonized PR was able to be effectively removed without pre-stripping by oxygen plasma ashing by using sc-$CO_2$ mixture containing the optimum formulated additives at the proper pressure and temperature, and the same result was also able to be obtained in the case of HDII wafer. It was found that the efficiency of SCPS of ion implanted wafer improved as the temperature of SCPS was high, so a very large amount of MEA in the sc-$CO_2$ mixture could be reduced if the temperature could be increased at condition that a process permits, and the ion implanted photoresist(IIP) on the wafer was able to be removed completely without pre-treatment of plasma ashing by using the only 1 step SCPS process. By using SCPS process, PR polymers formed on sidewalls of metal conductive layers such as aluminum films, titanium and titanium nitride films by dry etching and ashing processes were removed effectively with the minimization of the corrosion of the metal conductive layers.

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Channel Selective Relay-based Multiple-Input SC-FDMA/OFDMA Transmission System (채널 선택형 릴레이 기반 다중 입력 SC-FDMA/OFDMA 전송 시스템)

  • Won, Hui-Chul;Kim, Soon-Cheol
    • Journal of Korea Society of Industrial Information Systems
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    • v.14 no.5
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    • pp.1-9
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    • 2009
  • Relay-assisted multiple input technique has become a promising candidate for next generation broadband wireless communications. In this paper, we propose channel selective relay-based multiple input transmission system. In the proposed system, single carrier frequency division multiple access (SC-FDMA) and orthogonal frequency division multiple access (OFDMA) are adopted for uplink and downlink transmissions, respectively. The performance of relay-based system can be improved by using the subcarriers selectively based on the channel condition between relay station (RS) and mobile station, or between RS and base station. Simulation results show that the proposed relay-based system considerably outperforms the conventional relay-based system.

Effects of Dietary Yeast (Saccharomyces cerevisiae) Components on Growth Performance, Heal Morphology and Serum Cholesterol in Male Broiler Chickens (효모(Saccharomyces cerevisiae)의 급여가 육계의 생산성. 장내 융모 발달 및 혈청 콜레스테롤에 미치는 효과)

  • Lee B. D.;Zhang A. W.;Sung C. K.;Ahn G. H.;Lee K. W.
    • Korean Journal of Poultry Science
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    • v.32 no.1
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    • pp.49-54
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    • 2005
  • An experiment was conducted to investigate whether dietary yeast (Saccharomyces cerevisiae, SC) and its' structural components, i.e., yeast cell-extract (YE) and yeast cell-wall (CW) could influence growth performance, ileal morphology and serum lipids of male broiler chickens. There were four dietary treatments, each consisting of 6 replicates (10 birds per replicate). Chickens were fed a corn-soybean meal base control diet and diets containing SC ($0.5\%$), YE ($0.25\%$) and CW ($0.25\%$), respectively for 5-wk-experimental period. Dietary SC, YE and CW versus the control diet did not affect growth performance of male broiler chickens. Ileal morphology as to villus height, crypt depth and villus:crypt ratio of birds fed on the control diet was not significant from those fed on diets rich in SC, YE and CW, respectively. Dietary SC significantly lowered (P<0.05) serum total cholesterol by on average $19.7\%$ as compared to the control group. In addition, chickens fed on diets with either YE or CW lowered serum cholesterol by on average 15.3 and $12.5\%$, respectively as compared to the control albeit that the former only reached statistical significance. In conclusion, our study observed the hypocholesterolemic effect of SC in male broiler chickens. Moreover, YE, i.e., an extract of intracellular components of SC contains active molecules that are responsible far lowering serum cholesterol concentrations, but their identification at the molecular level needs to be assessed.

Separation of 2,6-dimethylnaphthalene in Dimethylnaphthalene Isomers Mixture by Crystallization Operation (결정화 조작에 의한 Dimethylnaphthalene 이성체 혼합물 중의 2,6-dimethylnaphthalene의 분리)

  • Kang, Ho-Cheol;Kim, Su Jin
    • Applied Chemistry for Engineering
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    • v.25 no.1
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    • pp.116-120
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    • 2014
  • Light cycle oil (LCO), one of the by-products of the catalytic cracking gasoline manufacturing process, contains a lot of valuable aromatics. In particular, 2,6-dimethylnaphthalene (2,6-DMN) contained in LCO has been becoming important as the basic material of polyethylene naphthalate plastic and liquid crystal polymer, etc. If it were possible to separate and purify the valuable aromatic hydrocarbons (such as 2,6-DMN) from LCO, which have only been used as fuel mixed with heavy oil, it would be very meaningful in terms of the efficient use of resources. We investigated the high-purity purification of 2,6-DMN by the combined method of melt crystallization (MC) and solute crystallization (SC). The enriched DMN isomer mixtures (concentration of 2,6-DMN : 10.43%) recovered from LCO by distillation-extraction combination and the crystal recovered by MC used as raw materials of MC and SC, respectively. The solvent of SC used was a mixture of methanol and acetone (60 : 40 wt%). The crystal of 2,6-DMN with a high-purity of 99.5% was recovered by MC-SC combination. We confirmed that the MC-SC combination was one of the very useful combinations for the high-purity purification of 2,6-DMN contained in the enriched DMN isomer mixtures.