• 한국연초학회지
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• 제23권2호
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• pp.133-140
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• 2001

PAP-I cDNA was synthesized from total RNA of Phytolacca americana leaves by RT-PCR, and then subcloned to recombinant vector pBluescript II SK-. Using PCR with primers designed in our laboratory, we could get the 9 deletion mutant PAP-I cDNA fragments. The first of the fragments was deleted by 66bp from immature N-terminal and then the rest were deleted by 90bp sequentially. Sequentially deletion mutant PAP-I cDNAs were inserted to pAc55M, on down-stream of gall promoter. Recombinant pAc55M was transformed to yeast cells, psy1 and the cells were spreaded on SC_urn-/glucose plate media. Colonies on SC_ura-/glucose plate were streaked on the same position of SC_ura-/glucose and SC_ura-/galactose plate, and we selected colonies growing on both plates, which carry non-cytotoxic deleted mutant PAP-I cDNA. We selected 4 deletion mutant PAP-I cDNAs which have not cytotoxicity.

• 한국균학회지
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• 제18권1호
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• pp.26-41
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• 1990

고마리 군락의 식물 뿌리 및 뿌리 주변 토양(土壤)에서 여러 가지 접합내생균근을 분리하였다. 분리된 포자의 형태 및 크기 등으로 동정한 결과 다음과 같이 4속 10종으로 분류 동정되었다 ; A. bireticulata, A. scroiculata, Gi. decipiens, Gi. gigantea, Gi. margarita, Gl. albidum, Gl. manihotis, Gl. pulvinatum, Sc. erythropa 및 Sc. gregaria.

• 산업공학
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• 제12권1호
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• pp.94-101
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• 1999

The set covering(SC) problem is the problem of covering all the rows of an $m{\times}n$ matrix of ones and zeros by a subset of columns with a minimal cost. It has many practical applications of modeling of real world problems. The SC problem has been proven to be NP-complete and many algorithms have been presented to solve the SC problem. In this paper we present hybrid simulated annealing(HSA) algorithm based on the Simulated Annealing(SA) for the SC problem. The HSA is an algorithm which combines SA with a crossover operation in a genetic algorithm and a local search method. Our experimental results show that the HSA obtains better results than SA does.

• 한국통신학회논문지
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• 제7권1호
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• pp.10-17
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• 1982

최근 switched capacitors 및 고정 capacitors와 능동소자를 利用한 analong 여파기 실현에 큰 관심을 갖게 되었다. Switched capacitor는 저항에 비해 온도특성 및 선형특성이 양호할 뿐 아니라 Si chip 면적이 훨씬 적게 소요되고 MOS기술에 의해 완전 IC화가 가능하다. 본 논문에서는 저항과 switched capacitor를 사용해 Gyrator 여파기내의 모든 저항을 switched capacitor로 대친시킬 SC-Gyrator 여파기를 실현했다. 실험결과 Gyrator여파기와 SC-Gyrator 여파기의 특성이 만족할 만큼 일치함을 확인했다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.699-703
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• 2005

An experiment was conducted to investigate the effects of various dietary levels of Saccharomyces cerevisiae (SC) on the growth performance and meat quality (i.e., tenderness and oxidative stability) of Ross broiler chickens. Two hundred and forty dayold broiler chicks were fed four experimental diets with graded levels of SC at 0.0, 0.3, 1.0 and 3.0%. Each treatment consisted of six cages with 10 chicks per cage. Feed and water were provided ad libitum throughout the experiment that lasted for 5 wk. Birds were switched from starter to finisher diets at 3 wk of age. The average BW gains of broiler chickens increased (linear p<0.05) during either 0-3 or 0-5 wk of age as dietary SC levels increased. A linear effect (p<0.05) of SC on feed intake during either 4-5 wk or 0-5 wk of ages was also monitored. The addition of SC to the control diet significantly lowered shear forces in raw breast, raw thigh, and boiled drumstick meats (linear p<0.05). Upon incubation, 2-thio-barbituric acid-reactive substances (TBARS) values increased gradually in breast and thigh meats while more dramatic increase was noted in skin samples. The TBARS values of either breast or thigh meats were not significantly affected (p>0.05) by dietary treatments up to 10 d of incubation. At 15 d of incubation, TBARS values of breast and thigh meats from all SC-treated groups were significantly lower (p<0.05) than those of the control. It appears that dietary SC could enhance growth performance of broiler chickens, and improve tenderness and oxidative stability of broiler meats.

• Journal of Microbiology
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• 제42권2호
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• pp.126-132
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• 2004

A single chain variable fragment (scFv) specific towards B. pseudomallei exotoxin had previously been generated from an existing hybridoma cell line (6E6AF83B) and cloned into the phage display vector pComb3H. In this study, the scFv was subcloned into the pComb3X vector to facilitate the detection and purification of expressed antibodies. Detection was facilitated by the presence of a hemagglutinin (HA) tag, and purification was facilitated by the presence of a histidine tag. The culture was grown at 30$^{\circ}C$ until log phase was achieved and then induced with 1 mM IPTG in the absence of any additional carbon source. Induction was continued at 30$^{\circ}C$ for five h. The scFv was discerned by dual processes-direct enzyme-linked immunosorbent assays (ELISA), and Western blotting. When compared to E. coli strains ER2537 and HB2151, scFv expression was observed to be highest in the E. coli strain Topl0F'. The expressed scFv protein was purified via nickel-mediated affinity chromatography and results indicated that two proteins a 52 kDa protein, and a 30 kDa protein were co-purified. These antibodies, when blotted against immobilized exotoxin, exhibited significant specificity towards the exotoxin, com-pared to other B. pseudomallei antigens. Thus, these antibodies should serve as suitable reagents for future affinity purification of the exotoxin.

• Journal of Dental Anesthesia and Pain Medicine
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• 제17권2호
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• pp.121-127
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• 2017

Background: There are no studies regarding 4% articaine infiltration injection into the retro-molar area for an impacted lower third molar (LITM) surgery. This study aimed to evaluate the efficacy of infiltration using 1.7 ml (single cartridge: SC) of 4% articaine versus 3.4 ml (double cartridges: DC) of 4% articaine with 1:100,000 epinephrine in LITM surgery. Method: This study involved 30 healthy patients with symmetrical LITM. The patients were assigned to receive either a DC or SC of 4% articaine with 1:100,000 epinephrine as a local anesthetic for each operation. Onset, duration, profoundness, need for additional anesthetic administration, total volume of anesthetic used, vitality of the tooth, and pain score during operation were recorded. Results: The DC of 4 % articaine had a significantly higher success rate (83.3%) than did the SC (53.3%; P<0.05). The duration of soft tissue anesthesia was longer in the DC group. The intra-operative pain was higher in the SC group with a significant (P < 0.05) requirement for a supplementary local anesthetic. Conclusion: We concluded that using DC for the infiltration injection had a higher success rate, longer duration of anesthesia, less intra-operative pain, and a lower amount of additional anesthesia than SC in the surgical removal of LITM. We recommend that a DC of 4% articaine and a 1:100,000 epinephrine infiltration in the retro-molar region can be an alternative anesthetic for LITM surgery.

• Journal of Yeungnam Medical Science
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• 제10권1호
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• pp.212-217
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• 1993

임상적으로 많이 이용되는 범위의 방사선 조사에서는 증식능의 상실을 세포사망으로 정의를 내린다. 본 연구는 실험용 orthovoltage조사기를 이용하여 확립된 세포주인 Hela.53(5C)를 대상으로 각각 100 cGy, 200 cGy, 400 cGy 및 600 cGy 방사선조사 후 2주일간 배양후 crystal violet 염색을 하여 육안적으로 판별가능한 크기의 집락의 수를 산출하여 다음의 결과를 얻었다. HeLa.S3(SC) 세포의 집락 형성능은 0.464이었다. 세포의 생존곡선의 형태는 다표적, 1 hit 형태를 보였다. Do는 150 cGy, Dq는 80 cGy, 외삽치는 1.7이었다. 이 연구결과는 향후 다양한 방사선 생물학 연구에 참고자료로 이용 가능하리라 사료된다.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.123-130
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• 2015

Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide ($SC-CO_2$) and different solvents. $SC-CO_2$ was kept at a temperature of $45^{\circ}C$ and pressure of 250 bar. The flow rate of $CO_2$ (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at $25^{\circ}C$ for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using $SC-CO_2$ with ethanol and different solvents have shown good activity. The highest activity belongs to $SC-CO_2$ with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of $68.38{\pm}1.21%$, $83.51{\pm}1.25%$, $0.64{\pm}0.02mg/g$, and $5.57{\pm}0.05mg/g$, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the $SC-CO_2$ extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.

• Nuclear Engineering and Technology
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• 제40권6호
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• pp.459-466
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• 2008

The KSTAR Magnet Power Supply (MPS) was dedicated to the SC coil commissioning and $1^{st}$ plasma experiment as a part of the system commissioning. Although many efforts to develop large-current power supplies that are useful for high power electronic devices have been made in various application fields, such as for large metal-plating devices, there were clear discrepancies between conventional power supply technologies and that for the SC coils due to the special SC coil load conditions. Therefore, most of the power supply technologies for the SC coils were a challenge in the domestic research area due to their limited application. However, the MPS commissioning result showed that all of the hardware and controlling software operated well, and this result finally led to the success of SC coil commissioning and the KSTAR $1^{st}$ plasma experiment. This paper will describe key features of KSTAR MPS for the $1^{st}$ plasma experiment, and will also report the commissioning results of the magnet power supplies.