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Antioxidant and Skin Whitening Effects of Rhamnus yoshinoi Extracts (짝자래나무 추출물의 항산화 및 미백 효과)

  • Seo, Eun-Jong;Hong, Eun-Suk;Choi, Min-Hee;Kim, Ki-Sun;Lee, Sung-Jun
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.750-754
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    • 2010
  • The purpose of this study was to investigate the antioxidant and skin whitening effects of Rhamnus yoshinoi extracts. Rhamnus yoshinoi was extracted with 100% ethanol and water. The antioxidative and skin whitening effects of extracts were determined by in vitro assays using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and inhibitory effects against tyrosinase activity and melanogenesis in B16F1 melanoma cells. The radical scavenging activities of the Rhamnus yoshinoi extracts were tested by DPPH assay and showed high DPPH radical scavenging activities (SC50; 21.6 ppm in EtOH, 40.5 ppm in water). As for tyrosinase inhibitory activity, the Rhamnus yoshinoi ethanol extract had the highest inhibition activity ($IC_{50}$; 256.3 ppm). In B16F1 mouse melanoma cells, the Rhamnus yoshinoi ethanol extract significantly inhibited melanin synthesis by 53.36% at the concentration of 50 ppm. These results suggest that Rhamnus yoshinoi ethanol extract has significant antioxidant activity and whitening activity.

Fabrication and Characteristics of High Efficiency Silicon PERL (passivated emitter and rear locally-diffused cell) Solar Cells (PERL (passivated emitter and rear locally-diffused cell) 방식을 이용한 고효율 Si 태양전지의 제작 및 특성)

  • Kwon, Oh-Joon;Jeoung, Hun;Nam, Ki-Hong;Kim, Yeung-Woo;Bae, Seung-Chun;Park, Sung-Keoun;Kwon, Sung-Yeol;Kim, Woo-Hyun;Kim, Ki-Wan
    • Journal of Sensor Science and Technology
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    • v.8 no.3
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    • pp.283-290
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    • 1999
  • The $n^+/p/p^+$ junction PERL solar cell of $0.1{\sim}2{\Omega}{\cdot}cm$ (100) p type silicon wafer was fabricated through the following steps; that is, wafer cutting, inverted pyramidally textured surfaces etching by KOH, phosphorus and boron diffusion, anti-reflection coating, grid formation and contact annealing. At this time, the optical characteristics of device surface and the efficiency of doping concentration for resistivity were investigated. And diffusion depth and doping concentration for n+ doping were simulated by silvaco program. Then their results were compared with measured results. Under the illumination of AM (air mass)1.5, $100\;mW/cm^2$ $I_{sc}$, $V_{oc}$, fill factor and the conversion efficiency were 43mA, 0.6 V, 0.62. and 16% respectively.

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Injection-Acupuncture Combined by Conventional Treatment in Canine Demodicosis : Case Report (개 모낭충증에 있어서 약침과 기존 치료의 병용 : 증례 보고)

  • Jun, Hyung-Kyou;Han, Ji-Won;Oh, Hyun-Wook;Lee, Hyun-Hwa;Park, Bae-Kun;Park, Seong-Jun;Cho, Sung-Whan;Kim, Duck-Hwan
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.432-436
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    • 2007
  • Therapeutic effect by injection-AP with antibiotics plus conventional treatment was investigated to establish a new treatment method for canine demodicosis. Three patients diagnosed into canine demodicosis were used in the present study. Injection-AP with enrofloxacin (5 mg/kg, twice a week) was made into the acupoints such as Fei Shu(BL13), Wei Zhong (BL40), Da Zhui (GV14), Zu San Li (ST36), He Gu (LI04), Qu Chi (LI11) and Sang Yin jiao(SP06) for 5 weeks. Further, they were additionally received with conventional treatments including injection of ivermectin (0.5 mg/kg, twice a week, SC) and 0.05% amitraz rinse (twice a week) for 5 weeks. The clinical symptoms (pruritus, excoriation, erythema and alopecia) were much improved by injection-AP therapy plus conventional treatment, compared by those of pre-treatment. Case 1 became to normal after 4 weeks, Case 2 became to mild after 5 weeks and Case 3 became to normal after 5 weeks. Total WBC revealed decreasing tendency with treatment in all cases, however, N/L ratios didn't show regular pattern of change with treatment. Notable changes were not histopathogically found in skin lesions of Case 1 and Case 3 except Case 2 with mild change after 5 weeks treatment, compared by those of pre-treatment, respectively. In conclusion, the present patients were cases with canine demodicosis which revealed favorable therapeutic response by injection-AP with antibiotics plus conventional treatment.

Herbicidal Effects on the Differential Rice Cultivation Condition and Damage of No-target Plants of Macro Granule Herbicide for Remote-controlled Aerial Application (무인헬기용 Macro granule 제초제의 벼 작형별 살초효과 및 주변작물에 미치는 영향)

  • Yoon, Cheol-Su;Bae, Chang-Hyu;Lee, Sheong-Chun;Kim, Kyung-Hyun;Lee, Kye-Hwan;Cho, Tae-Kyoung;Hwang, In-Cheon
    • Korean Journal of Weed Science
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    • v.31 no.4
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    • pp.375-383
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    • 2011
  • This experiment was carried out to confirm characteristics of macro granule (GG) for herbicidal efficacy by using remote-controlled aerial application (RCAA) to control annual and perennial weeds in rice paddy field, and phytotoxicity to rice and non target plants of formulation types. Herbicidal efficacy of GG applied by using RCAA was 93.6~96.6% in flooded direct sowing cultivation. There was no difference in phytotoxicity between the hand and the power applicator methods in terms of height, number of tiller and yield component. Herbicidal efficacy of GG applied by using RCAA was 92.7~97.3% in machine transplanting rice field. There was no difference in phytotoxicity between the hand and RCAA methods in terms of height, number of tiller and yield component. When suspension concentrate (SC) and GG were applied directly on Chinese cabbage, lettuce, cucumber, pepper, soybean and wild sesame, several symptoms of injury such as, the inhibition of growth, yellowish, leaf fall and withering was observed.

Fact-finding Survey on Occurrence of Paddy Field Weeds and The Use of Paddy Field Herbicides at Farmer's Level in Korea (논잡초 발생양상 및 논 제초제 사용 실태조사)

  • Kim, Chang-Seok;Lee, Jeongran;Won, Tae-Jin;Seo, Young-Ho;Kim, Eun-Jung;Lee, Sun-Gye;Cho, Seung-Hyun;Kwon, Oh-Do;Kim, Sang-Kuk;Chung, Wan-Gyu;Park, Tae-Seon;Moon, Byeong-Chul;Park, Jae-Eup;Lee, In-Yong
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.6-12
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    • 2012
  • A fact-finding survey was conducted to obtain the basic information on nation-wide weed distribution and farmer's opinions to weed control strategy in paddy field. Most respondents considered Echinochloa spp., Monochloa vaginalis, Sagittaria trifolia, and Scirpus juncoides etc. as dominate weeds in paddy fields. And herbicide resistant weed species were M. vaginalis, S. juncoides, E. oryzicola and S. pygmaea etc. Purchase guidances for herbicide selection, experience of farmers was 34.0% and dealers recommendation was 33.9% at farmer's level. And paddy field herbicides application frequence were one time 15.6% but twice 75.6%. Preferred herbicides were ranked in the following order: oxadizone 12% EC, butachlor 5% GR, butachlor 33% CS, mefenacet pyrazodulfuron-ethyl 21.42% SC, and oxadiargyl 1.7% EC etc. in paddy fields. And in most preferred foliar herbicide were bentazone MCPA 38.6% SL and bentazone cyhalop-butyl 18.5% ME, 48.5% and 22.3%, respectively.

Studies for Antibiotic Free Chicken Production Using Water Extracts from Artemisia capillaris and Camellia sinensis (인진쑥 및 녹차 추출물을 이용한 무항생제 닭고기 생산 연구)

  • Kim, Dong-Wook;Kim, Ji-Hyuk;Kang, Geun-Ho;Kang, Hwan-Ku;Park, Sung-Bok;Park, Jae-Hong;Bang, Han-Tae;Kim, Min-Ji;Na, Jae-Cheon;Chae, Hyun-Suk;Choi, Hee-Chul;Suh, Ok-Suk;Kim, Sang-Ho;Kang, Chang-Won
    • Food Science of Animal Resources
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    • v.30 no.6
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    • pp.975-988
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    • 2010
  • Two experiments were conducted to determine whether water extracts from Artemisia capillaries (A. capillaries) and Camellia sinensis (C. sinensis) could be used as alternatives to antibiotic growth promoters in broiler feed. The experiment 1 was verified their chemical composition, extracts yields, total phenolic compounds concentration, antioxidant activity, antimicrobial activity, and chicken splenocytes proliferation through in vitro test. The extract yields of A. capillaries and C. sinensis were 26.5 and 16.8%, respectively. Total phenolic compounds concentrations of them expressed as gallic acid equivalent were 15.28 and 26.74 mg/mL, respectively. Electron donating abilities of them expressed as $SC_{50}$ showing 50% DPPH radical scavenging were 0.30 and 0.06 mg, respectively. Bacterial inhibitory rates of them against Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium were ranged from 42.1 to 52.3% and from 21.6 to 33.7%, respectively. And, these extracts increased proliferation of chicken splenocytes. Especially, A. capillaris was more excellent than Echinacea and Concanavalin A known as T-cell stimulator. The experiment 2 was investigated their effects on growth performance, relative organ weight, cecal microflora, blood biochemical parameters, and splenic cytokines mRNA expression in broiler chicks. Four hundred eighty 1-day-old male broiler chicks (Ross 308) were divided in to 4 treatment groups with 4 replicates of 30 birds in each group: NC (control, no antibiotics), PC (avilamycin, 10 ppm; salinomycin, 60 ppm), AC (A. capillaries, 100 ppm), and CS (C. sinensis, 100 ppm); treatments were administered through water supplementation. Final body weight was significantly higher in all treated groups than in NC (p<0.05). Cecal Salmonella numbers were significantly or somewhat decreased in all treated groups than in NC (p<0.05). The relative weights and lengths of the small intestine were more significantly decreased in the PC and AC groups than in the other groups. Cecal Salmonella numbers were significantly or somewhat decreased in all treated groups than in the NC group (p<0.05). The contents of total cholesterol, aspatate aminotransferase, and alanine aminotransferase in blood serum were more significantly decreased in all treated groups than in NC (p<0.05). In conclusion, these results suggested the possibility that these extracts could serve as alternatives for antibiotic growth promoters.

Selection of Virulent Isolates of Fusarium circinatum and Investigation of Pitch Canker Severity of Pinus rigida and P. rigida × P. taeda Seed Orchards in Jeju Island (제주도(濟州道) 리기다 및 리기테다 채종원(採種園)에서 푸사리움가지마름병 피해도(被害度) 조사(調査) 및 병원성(病原性) 균주선발(菌株選拔))

  • Woo, Kwan-Soo;Kim, Young-Joung;Kim, Tae-Su;Lee, Seong-Kyu
    • Journal of Korean Society of Forest Science
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    • v.94 no.6
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    • pp.402-409
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    • 2005
  • This study was undertaken to compare and estimate the severity of pitch canker of individual trees of Pinus rigida and Pinus rigida ${\times}$ P. taeda in two seed orchards in Jeju island, in which the orchards have been damaged by the pitch canker for seven years. Wind-pollinated two-year-old seedlings of P. rigida and P. rigida ${\times}$ P. taeda, in which the seedlings of P. rigida ${\times}$ P. taeda were from seeds of phenotypically selected, uninfected(but untested) trees, were inoculated with the pathogenic fungus, Fusarium circinatum, isolated from P. rigida and P. thunbergii. The virulence of the isolates was also identified. Statistically significant difference was found in 'stem cankers'(SC; ${\chi}^2=7.76$, P=0.05) among 4 plantations of P. rigida ${\times}$ P. taeda of two seed orchards. P. rigida was higher in 'top kill' (TK) and 'branch tip symptoms' (BT) than those of P. rigida ${\times}$ P. taeda. In artificial inoculation tests, mortality of the seedlings from the resistant candidates was 14% higher than that of the seedlings from the susceptible candidates. This result may becaused by unknown pollen trees and/or candidate tree selection based only on phenotype. Two of five fungal isolates, C-6-L(9) and C-6-L(19), showed significantly higher mortality (68% and 60%, respectively) than others, suggesting that these isolates can be used as virulent isolates for a mass artificial inoculation. Resistance candidate seedlings that were selected from this study can be utilized as useful materials for fundamental studies of genetics and biochemistry to breed resistance varieties to pitch canker.

A Faster Approach to Stereocomplex Formation of High Molecular Weight Polylactide Using Supercritical Dimethyl Ether (디메틸에테르 초임계 유체를 이용한 고분자량 폴리락티드 스테레오 콤플렉스의 제조)

  • Bibi, Gulnaz;Jung, Youngmee;Lim, Jong Choo;Kim, Soo Hyun
    • Polymer(Korea)
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    • v.39 no.3
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    • pp.453-460
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    • 2015
  • Engineering the polylactide via stereocomplexation with supercritical fluid (SCF) technology paved way to fabricate polymers with enhanced thermal and mechanical properties. We aimed to establish a SCF medium with excellent solubility for PLA without any additional solvent/co-solvent. We, therefore, employed supercritical dimethyl ether to synthesize 100% stereocomplex polylactide from high molecular weight homopolymers with an excellent yield. The remarkable solubility of the homopolymers in dimethyl ether is the key for quick conversion to s-PLA. This study proves a rapid synthesis route of dry s-PLA powder with sc-DME at 250 bar, $70^{\circ}C$ and 1.5 h, which are reasonably achievable processing parameters compared to the conventional methods. The degree of stereocomplexation was evaluated under the effect of pressures, temperatures, times, homopolymer-concentrations and molecular weights. An increment in the degree of stereocomplexation was observed with increased temperature and pressure. Complete conversion to s-PLA was obtained for PLLA and PDLA with $M_n{\sim}200kg{\cdot}mol^{-1}$ with a total homopolymer to total DME ratio of 6:100% w/w at prescribed reaction conditions. The degree of stereocomplexation was determined by DSC and confirmed by XRD. Considerable improvement in thermo-mechanical properties of s-PLA was observed. DSC and TGA analyses proved a $50^{\circ}C$ enhancement in melting transition and a high onset temperature for thermal degradation of s-PLA respectively.

Comparison Study of the Modulation Transfer Function of a Prototype a-Se based Flat Panel Detector with Conventional Speed Class 400 Film/screen System (비정질 셀레늄을 이용한 직접방식의 디지털 방사선 검출기와 X-ray film과의 MTF측정을 통한 영상 질(quality) 비교평가에 관한 연구)

  • Park, Jang-Yong;Park, Ji-Koon;Kang, Sang-Sik;Moon, Chi-Woong;Lee, Hyung-Won;Nam, Sang-Hee
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.40 no.3
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    • pp.163-171
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    • 2003
  • To evaluate the performance of the digital radiography(DR) system developed in our group, the modulation transfer function(MTF) was measured and compared with that of an analog X- ray detector, film/screen system. The DR system has an amorphous selenium(a-Se) layer vacuum-evaporated on a TFT flat panel detector. The speed class 400 film/screen (Fuji) system has been being used in the clinical field as analog X-ray detectors. Both the square wave and slit method were used to evaluate their MTF. The square method was applied to both film/screen and the DR system. The slit method, however, was applied to only DR system. The full-width half maximum resolution of film/screen was 357${\mu}{\textrm}{m}$(1.4 lp/mm at 50% spatial frequency), and the resolution of DR was limited to 200${\mu}{\textrm}{m}$(2.5 lp/mm at 30%). These results indicate the measured resolution limitations approximate to the pixel pitch, 139 ${\mu}{\textrm}{m}$ of TFT. The MTF of DR is higher than that of film/screen by the factor of 1.785. It is proved that our a-Se based DR system has potential usefulness in the clinical field.

Primary Dilated Cardiomyopathy in a Miniature Pinscher Dog (미니어쳐 핀셔견에서 발생한 확장성 심근질환)

  • Kim, Jung-Hyun;Park, Chul;Ko, Ki-Jin;Kang, Byeong-Teck;Jung, Dong-In;Kim, Ju-Won;Kim, Ha-Jung;Lim, Chae-Young;Lee, So-Young;Cho, Sue-Kyung;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.23 no.3
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    • pp.349-354
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    • 2006
  • A 9-month-old, female Miniature Pinscher(MP) dog weighing 1.97kg was presented because of periodic syncopal episode for 5 months. This case was diagnosed as primary dilated cardiomyopathy based on respiratory distress history, weak femoral pulse, generalized cardiomegaly, pulmonary edema, marked dilation of left atrium(LA) and left ventricle(LV), decreased wall thickness of LV and interventricular septum(IVS), increased EPSS in echocardiography, and young age of onset in the absence of other cardiovascular disorders. The patient was stabilized by application of diuretics(Furosemide, 2 mg/kg, SC, q 1 hr) and venodilator(Nitroglycerine patch, 0.5 mg/kg, q 12 hrs). Clinical signs were improved with medical management of positive inotropic vasodilator(Pimobendan, 0.2 mg/kg, PO, q 12 hrs) and angiotensin-converting enzyme(ACE) inhibitor(benazepril, 0.5 mg/kg, PO, q 12 hrs), potassium gluconate gel(2 mEq/dog, PO, q 12 hrs) and, L-carnitine(50 mg/kg, PO, q 12 hrs). The dog still maintains stable clinical status 10 months after the first visit. We report the rare case of DCM in small breed dog, which corresponds to the diagnosis and treatment of typical DCM in large breed dog.