• Journal of Microbiology and Biotechnology
• /
• v.19 no.11
• /
• pp.1306-1318
• /
• 2009

The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes. Two $\alpha$-amylases ScAmy54 and ScAmy43 predicted to play an important role in starch degradation were showed to produce specific oligosaccharides essentially maltotriose that have a considerable commercial interest. Primary structure of the two enzymes was established by N-terminal sequencing, MALDI-TOF masse spectrometry and cDNA cloning. The two proteins have the same N-terminal catalytic domain and ScAmy43 derived from ScAmy54 by truncation of 96 amino acids at the carboxyl-terminal region. Data of genomic analysis suggested that the two enzymes originated from the same $\alpha$-amylase gene and that truncation of ScAmy54 to ScAmy43 occurred probably during S. sclerotiorum cultivation. The structural gene of Scamy54 consisted of 9 exons and 8 introns, containing a single 1,500-bp open reading frame encoding 499 amino acids including a signal peptide of 21 residues. ScAmy54 exhibited high amino acid homology with other liquefying fungal $\alpha$-amylases essentially in the four conserved regions and in the putative catalytic triad. A 3D structure model of ScAmy54 and ScAmy43 was built using the 3-D structure of 2guy from A. niger as template. ScAmy54 is composed by three domains A, B, and C, including the well-known $(\beta/\alpha)_8$ barrel motif in domain A, have a typical structure of $\alpha$-amylase family, whereas ScAmy43 contained only tow domains A and B is the first fungal $\alpha$-amylase described until now with the smallest catalytic domain.

• Journal of Korean Society of Steel Construction
• /
• v.22 no.5
• /
• pp.455-463
• /
• 2010

This paper describes the experimental study on the mechanical characteristics of a steel plate-reinforced concrete joint. As an alternative reinforced concrete structure, the SC modular construction method is widely used and studied in the field ofindustrial facility field. However, the structure characteristics of RC and SC joint are not yet studied completely. In this paper, the beam-type construction joint of RC and SC walls was made to simulate the application of SC module to the large RC structure. Also, an out-of-plane loading was applied to the test specimen in order to evaluate flexural strength and structural properties of the beam-type RC-SC joint.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.23 no.8
• /
• pp.981-987
• /
• 2019

In this paper, we propose a new SC-FDE (single carrier frequency domain equalization) structure to cope with narrow band interference signals or jammers and reduce pilot overhead. The conventional SC-FDE structure has a problem that the receiver performance degrades severely due to difficulty in time-domain channel estimation when narrow band jammers exist. In addition, the spectral efficiency is lowered by transmitting pilot at every SC-FDE block to estimate channel response. In order to overcome those problems, the proposed structure is devised to estimate frequency domain channel directly without time domain channel estimation. To reduce the pilot overhead, several data blocks are transmitted between two pilots. The channel estimate of each data block is found through linear interpolation of two channel estimates at two pilots. By performing frequency domain channel equalization using this channel estimate, the distortion by the channel is well compensated when narrow band jammers exist. The performance of the proposed structure is confirmed by computer simulation.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.32 no.5A
• /
• pp.277-287
• /
• 2012

The shear wall with openings built with reinforced concrete (RC) have been elaborately studied by many researchers, whereas the steel plate concrete (SC) wall structure has not been investigated as much. The recent SC wall structures developed in Korea have been partly applied to nuclear power plant structures, although its design specification or guideline for the SC wall structure with openings has not been completed yet. This study based on numerical analysis evaluates the effects of opening on the structural resistance of the SC structure in nuclear power plant. As a result from nonlinear analysis, since the strengthening for openings significantly affect the overall strength of SC wall, the openings should be considered to strengthen them around adjacent area. It is also proved that the strengthened openings have the sufficient resistance and ductility regardless their size, shape, location, and quantity.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.5
• /
• pp.661-671
• /
• 2018

Domestic studies on steel plate concrete structures have focused on nuclear structures with high strength. In this study, the SC structure was applied to the general structure, and the SC structure that is advantageous in terms of safety and construction was limited to a special structure. As a basic study for applying SC, this paper proposes basic design information of a SC structure applying cement concrete to plan the structure, which is suitable for eco - friendliness by replacing concrete cement, an important factor in a SC structure, with blast furnace slag. This study examined the compression characteristics and the effective length factor under central compression load. To calculate the effective length factor, the Euler column theory was applied without applying plate theory. The effective length factor was calculated from the yield strength of the steel plate, buckling of the steel plate, and the point at which the concrete was broken. In addition, this study examined whether the maximum compressive strength meets the national and international reference equations with the slenderness ratio (B/t) as a parameter. By analyzing the buckling of the specimen by applying the column theory and selecting the strain of the measured steel plate, the effective length factor was analyzed and compared with the value presented in the reference equation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.26 no.8
• /
• pp.575-581
• /
• 2013

The self-cascode (SC) structure has low output voltage swing and high output resistance. In order to implement a simple and better SC structure, the native-$V_{th}$ MOSFETs which has low threshold voltage($V_{th}$) is applied. The proposed SC structure is designed using a qualified industry standard $0.18-{\mu}m$ CMOS technology. Measurement results show that the proposed SC structure has higher transconductance as well as output resistance than single MOSFET. In addition, analog building blocks (e.g. current mirror, basic amplifier circuits) with the proposed SC structure are investigated using by Cadence Spectre simulator. Simulation results show improved electrical performances.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.5
• /
• pp.529-535
• /
• 2011

Single-chain variable fragment (scFv) is a fusion protein of the variable regions of the heavy (VH) and light (VL) chains of immunoglobulin, connected with a short linker peptide of 10 to about 20 amino acids. In this study, the scFv of a monoclonal antibody against the third domain of human CD4 was cloned from OKT4 hybridoma cells using the phage display technique and produced in E. coli. The expression, production, and purification of anti-CD4 scFv were tested using SDS-PAGE and Western blot, and the specificity of anti-CD4 scFv was examined using ELISA. A 31 kDa recombinant anti-CD4 scFv was expressed and produced in bacteria, which was confirmed by SDS-PAGE and Western blot assays. Sequence analysis proved the ScFv structure of the construct. It was able to bind to CD4 in quality ELISA assay. The canonical structure of anti-CD4 scFv antibody was obtained using the SWISS_MODEL bioinformatics tool for comparing with the scFv general structure. To the best of our knowledge, this is the first report for generating scFv against human CD4 antigen. Engineered anti-CD4 scFv could be used in immunological studies, including fluorochrome conjugation, bispecific antibody production, bifunctional protein synthesis, and other genetic engineering manipulations. Since the binding site of our product is domain 3 (D3) of the CD4 molecule and different from the CD4 immunological main domain, including D1 and D2, further studies are needed to evaluate the anti-CD4 scFv potential for diagnostic and therapeutic applications.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.2A
• /
• pp.169-178
• /
• 2007

In this paper, we propose a signal structure and its optimum receiver to improve performance of SC-FDE(Single Carrier with Frequency Domain Equalization) system. Conventional SC-FDE systems have a drawback of power unbalance in frequency domain due to generation of pilot signals in time domain. The unbalanced power in frequency domain induces a channel estimation error and the performance of the receiver is degraded significantly. To overcome the drawback we apply CAZAC sequence which has constant power distribution in time and frequency domain. We design the signal structure to improve the performance with the repeated CAZAC sequence, and we design a receiver to optimize the proposed structure. Computer simulation results show that the proposed structure is superior to the conventional structure considering frame synchronization, frequency synchronization and channel equalization on typical wireless mobile channel environment.

• Journal of the Korean Society for Heat Treatment
• /
• v.35 no.3
• /
• pp.150-158
• /
• 2022

The effects of scandium addition on the Al-6Si-2Cu Alloy were investigated. The Al-6Si-2Cu-Sc alloy was prepared by gravity die casting process. In this study, scandium was added at 0.2 wt%, 0.4 wt%, 0.8 wt%, and 1.0 wt%. The microstructure of Al-6Si-2Cu-Sc alloy was investigated using Optical Microscope, Field Emission Scanning Electron Microscope, Electron Back Scatter Diffraction, and Transmission Electron microscope. The microstructure of Al-6Si-2Cu alloy with scandium added changed from dendrite structure to equiaxed crystal structure in specimens of 0.4 wt% Sc or more, and coarse needle-shape eutectic Si and β-Al₅FeSi phases were segmented and refined. The nanosized Al₃Sc intermetallic compound was observed to be uniformly distributed in the modified Al matrix.

• Journal of Korean Society of Steel Construction
• /
• v.16 no.3 s.70
• /
• pp.377-385
• /
• 2004

An experimental and analytical study on the behavior of the wall-support joint in SC(steel plate-concrete) structure was performed. Nine full-scale specimens were tested with a horizontal monotonic load, all acting in the same plane, causing a uni-axial moment on the SC structure's wall-support beam joint. The main focus is to examine thenonlinear behavior and ultimate strength of the SC wall-support joint. The effects of parameters, such aslocation of support, thickness of the steel plate, and size of support, were studied. The yield strength and ultimate strength of the plate-concrete wall was defined by examining the load-deflection relationship, showing the tension membrane action.