• BMB Reports
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• 제44권9호
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• pp.584-589
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• 2011

The mammalian ste20-like kinase (MST) pathway is important in the regulation of apoptosis and cell cycle and emerges as a novel tumor suppressor pathway. MST-induced phosphorylation of Salvador homolog 1 (SAV1), which is a scaffold protein, has not been evaluated in detail. We performed a mass spectrometric analysis of the SAV1 protein that was co-expressed with MST2. Phosphorylation was detected at Thr-26, Ser-27, Ser-36 and Ser-269. Although single or double mutations had little effects, the mutation of all four residues in SAV1 to Ala (SAV1-4A) had inhibitory effects on the MST pathway. MST2-mediated induction of SAV1-4A protein levels, SAV1-4A interaction with MST2 and the self-dimerization of SAV1-4A were weaker compared to those of wild-type SAV1. SAV1-4A inhibited MST2- and K-RasG12V-induced cell death of MCF7 cells. These results suggest that MST-mediated phosphorylation of four residues within SAV1 may be important in the induction of cell death by the MST pathway.

• 한국어병학회지
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• 제31권2호
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• pp.57-70
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• 2018

Infection with salmonid alphavirus (SAV) is a serious disease that mainly affects rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar L.) reared in seawater or freshwater. SAV is prevalent in European countries including Norway that exports rainbow trout and Atlantic salmon to Korea. Consequently, SAV was listed as a disease notifiable to the OIE and many salmonid-producing countries either designate SAV as their notifiable disease or do research on the development of diagnosis and epidemiology to reduce the possibility of SAV infection. Unlike other salmonid-producing countries, SAV is not listed as a notifiable disease in Korea, thereby arousing concern that SAV will get into the country through the importation of live salmonids. Under the circumstance, Korea needs to have a legal basis to take much stricter follow-up measures, including listing SAV as a notifiable disease, establishing surveillance system based on OIE standards to declare Korea free from SAV, killing infected fish and conducting fallow system against affected farms.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.136-139
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• 2009

Avermectin and its analogs are major commercial antiparasitic agents in the fields of animal health, agriculture, and human infections. Previously, comparative transcriptome analysis between the low-producer S. avermitilis ATCC31267 and the high-producer S. avermitilis ATCC31780 using a S. avermitilis whole genome chip revealed that 50 genes were overexpressed at least two-fold higher in S. avermitilis ATCC31780. To verify the biological significance of some of the transcriptomics-guided targets, five putative regulatory genes were individually cloned under the strong-and-constitutive promoter of the Streptomyces expression vector pSE34, followed by the transformation into the low-producer S. avermitilis ATCC31267. Among the putative genes tested, three regulatory genes including SAV213, SAV3818, and SAV4023 exhibited stimulatory effects on avermectin production in S. avermitilis ATCC31267. Moreover, overexpression of SAV3818 also stimulated actinorhodin production in both S. coelicolor M145 and S. lividans TK21, implying that the SAV3818, a putative TetR-family transcriptional regulator, could be a global upregulator acting in antibiotic production in Streptomyces species.

• 화약ㆍ발파
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• 제25권1호
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• pp.31-43
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• 2007

현재 대부분의 국내 터널은 천공발파 기법을 이용하여 굴착되고 있다. 심발발파는 터널굴착면의 1 자유면 상태에서 가장 먼저 발파가 이루어지므로 터널발파에서 가장 중요한 단계이며 전체의 굴진효율과 진동 및 소음을 결정하는 역할을 한다. 여기서는 기존의 V-Cut 공법의 시공 편의성을 최대한 활용하면서 굴진효율을 개선시킨 새로운 심발발파 방법인 SAV-Cut (Stage Advance V-Cut) 공법을 소개한다. SAV-Cut 공법은 건설교통부에서 지정하는 건설 신기술(제518호)으로서 중앙공을 천공 및 선기폭하여 암반을 약화시킨 다음 경사공을 단계별로 기폭하는 것을 주요 특징으로 하고 있다. SAV-Cut 발파공법의 메커니즘을 수치해석과 여러 현장에 실제 적용을 통하여 검증하였고, 발파효율의 증대와 진동저감 효과를 확인하였다.

• 한국자기공명학회논문지
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• 제17권1호
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• pp.54-58
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• 2013

SAV0506 is an 87 residue hypothetical protein from Staphylococcus aureus strain Mu50 and also predicted to have similar function to ribosome associated heat shock protein, Hsp 15. Hsp15 is thought to be involved in the repair mechanism of erroneously produced 50S ribosome subunit. In this report, we present the sequence specific backbone resonance assignment of SAV0506. About 82.5% of all resonances could be assigned unambiguously. By analyzing deviations of the $C{\alpha}$ and $C{\beta}$ chemical shift values, we could predict the secondary structure of SAV0506. This study is an essential step towards the structural characterization of SAV0506.

• 한국자기공명학회논문지
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• 제16권2호
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• pp.162-171
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• 2012

SAV2228 has an OB (Oligomer-Binding)-motif which is frequently used for nucleic acid recognition. To characterize the activity of translation initiation factor-1 (IF-1) from Staphylococcus aureus, SAV2228 was expressed and purified in Escherichia coli. We acquired 3D NMR spectra showing well dispersed and homogeneous signals which allow us to assign 94.4% of all $^1HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$ and $^{13}CO$ resonances. We could predict a secondary structure of SAV2228 using TALOS and CSI from NMR data. SAV2228 was consisted of one ${\alpha}$-helix and five ${\beta}$-sheets. The predicted secondary structure, ${\beta}-{\beta}-{\beta}-{\alpha}-{\beta}-{\beta}$, was similar to other bacterial IF-1, but it was not completely same to the eukaryotic one. Assigned NMR peaks and secondary structre prediction can be used for the study on interaction with nucleic acid in the future.

• Nuclear Engineering and Technology
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• 제26권2호
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• pp.247-256
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• 1994

SAV90에서 사용되고 있는 핵종 연쇄모델을 CASMO3/MEDIUM3 계산절차에 상응되도록 수정하였다. 기존의 핵종 연쇄모델은 21개의 핵종으로 표현되어 있어 CASMO3의 계산결과를 MEDIUM3에서 그대로 구현하는데 충분치 않은 것으로 밝혀졌다. 따라서, 이를 해결하기 위해서 기존의 핵종 연쇄모델을 수정 확장시켰으며, 여기에서 분석된 여러 핵종 연쇄모델들중 21 핵종 연쇄모델에 Pu238만을 더 고려한 22 핵종을 가진 연쇄모델이 정확도와 계산효율을 모두 고려할 때 가장 우수한 것으로 나타났다. 이 모델을 이용하여 영광 1호기의 노심연소계산을 수행하였으며, 이를 주요 노심 측정치와 비교한 결과 잘 일치하는 것으로 나타났다.

• Nuclear Engineering and Technology
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• 제53권10호
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• pp.3398-3405
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• 2021

This study observed the effect of neutron irradiation and ageing on the microstructure, hardness, and corrosion resistance of SAV-1 (Al-Mg-Si) alloy. The investigated material was irradiated with neutrons to fluences of 10²¹-10²⁶ n/m² in the WWR-K research reactor and kept in dry storage. Long-term irradiation led to an increase in hardness of the alloy and a deterioration of pitting corrosion resistance. Post-irradiation ageing for 1 h at 100-300 ℃ resulted in a decrease in microhardness of the irradiated SAV-1. The effect of post-irradiation ageing on pitting corrosion was made clear through the formation of Guinier-Preston zones and secondary precipitates in the Al matrix. Ageing at 250 ℃ corresponded to the development of stable microstructure and the highest corrosion resistance for the irradiated samples. Mg₂Si, Si, and needle-shaped β" precipitates were formed in SAV-1 alloy that was irradiated with low fluences. β" and clusters of rod-shaped B-type precipitates were observed in highly irradiated samples. The precipitates were similar to those seen in non-irradiated pseudo-binary Al-Mg₂Si alloys with Si excess.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.500-505
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• 2019

Staphylococcus aureus is a round-shaped, gram-positive bacterium that can cause numerous infectious diseases ranging from mild infections such as skin infections and food poisoning to life-threatening infections such as sepsis, endocarditis and toxic shock syndrome. Various antibiotic-resistant strains of S. aureus have frequently emerged, threatening human lives significantly. Despite much research on the genetics of S. aureus, many of its genes remain unknown functionally and structurally. To counteract its toxins and to prevent the antibiotic resistance of S. aureus, our understanding of S. aureus should be increased at the proteomic scale. SAV0927 was first sequenced in an antibiotic resistant S. aureus strain. The gene is a conserved hypothetical protein, and its homologues appear to be restricted to Firmicutes. In this study, we determined the crystal structure of SAV0927 at $2.5{\AA}$ resolution. The protein was primarily dimeric both in solution and in the crystals. The asymmetric unit contained five dimers that are stacked linearly with ${\sim}80^{\circ}$ rotation by each dimer, and these interactions further continued in the crystal packing, resulting in a long linear polymer. The crystal structures, together with the network analysis, provide functional implications for the SAV0927-mediated protein network.

• 한국수산과학회지
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• 제55권3호
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• pp.328-337
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• 2022

This study investigated the removal performance of a foam fractionator under seawater conditions. The foam fractionator was tested using a 3×3×3 factorial design for operating conditions by combining different solids concentrations (SS; 1, 5, and 10 mg·L^-1), surface air velocities (SAV; 1.1, 1.5, and 2.1 cm·sec^-1), and hydraulic residence times (HRT; 1, 3, and 6 min) at 16℃. Performance parameters such as daily solids removal rate and efficiency were measured, and a multi-regression model equation was developed accordingly. The daily solids removal rate and removal efficiency varied with the experimental conditions and ranged from 0.14-2.33 g-solids·m^-3-air·day^-1 and 8.9-96.7 %, respectively. Overall, the daily solids removal rate increased with increasing SS and SAV and decreasing HRT, whereas the removal efficiency increased with increasing SAV and HRT and decreasing SS. The daily solids removal rate (g-solids·m^-3-air·day^-1) of the foam fractionator for SAV (cm·sec^-1), SS (mg·L^-1) and HRT (min) were described by the following multi-regression model: Daily solids removal rate [f(z)]=-0.118+0.422SAV+0.094HRT+0.141SS (r²=0.873).