• 생명과학회지
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• 제6권3호
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• pp.178-184
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• 1996

Aloe(Aloe vera LINNE) has been used as a home medicine for the past several thousand in the world, and has been studied on various chronic degenerative diseases such as atherosclerosis, myocardiac infarction and hypertension. SMAP8, learning and memory impairment animal mode, were fed basic or experimental diets with 1.0% of freeze dried(FD)-Aloe powder for 8 months. This study was designed to investigate the effects of Aloe on body weight gain, grading score of senescence(GSS), triglyceride, total and LDL-cholesterol levels, and atherogenic index in serum of SAMP8, and also designed to investigate the effects of Aloe on cholesterol accumultions in mitochondria and microsome fractions of SAMP8 brain. Body weight gain was consistently lower in aloe group than in control group, but no significantly differences between them. Grading score of senescence resulted ina marked decreases pf 20% in 1.0% Aloe group compared with control group. Administrations of 1.0% aloe resulted ina marked decreases in 15% and 20% of triglyceride and cholesterol levels, respectively, and also significantly decreased in 15% of LDL-cholesterol levels and atherogenic index in serum of SAMP8 compared with control group. Cholesterol accumulations were significantly inhibited in 20% and 10% of mitochondria and microsome fractions of SAMP8 brain, respectively, by administration of 1.0% Aloe. These results suggest that administration of Aloe mau not only effectively inhibit chronic degenerative diseases in serum of SAMP8, but may also improve learning and memory impairments of SAMP8 brain.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1311-1315
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• 2009

The objective of this study was to investigate the effects of sesaminol glucosides (SG) on age-related cognitive deficits in senescence-accelerated mice P8 (SAMP8). Male SAMP8 (9 month-old) were randomly divided into 3 groups and received diets containing 0, 0.25, or 0.5% SG for 12 weeks. Step-through latency of the SAMP8 control group was higher than that of the senescence-accelerated resistant mice (SAMR) group, whereas it was lowered in the SG-supplemented group on the passive-avoidance test. In the Morris water maze, the escape latency of the SAMP8 control group was increased and recovered in the 0.5% SG-supplemented group. The SG supplementation significantly decreased thiobarbituric acid reactive substance (TBARS) levels in brains of the SAMP8. On the other hand, catalase, superoxide dismutase, and glutathione peroxidase activities in brains of the SG supplemented group decreased compared with the SAMP8 control group. These results suggest that SG could attenuate cognitive deficits caused by aging through its antioxidant capacity.

• 한국식품과학회지
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• 제36권4호
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• pp.637-642
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• 2004

본 연구는 탈지 참깨 및 탈지 들깨의 메탄올추출물이 기억과 학습능력 감퇴의 특성을 가지고 있는 노화촉진마우스 SAMP8의 인지기능 및 항산화체계에 미치는 영향을 검토하기 위하여 각 추출물 0.3% 첨가 식이로 3개월간 섭취시킨 후 passive avoidance test로 인지기능변화를 측정하였고, 뇌중 acetylcholinesterase 활성, 지질과산화물 및 항산화효소활성을 분석하였다. 그 결과, 탈지 참깨 및 탈지 들깨의 메탄올 추출물이 노화촉진으로 감소한 step through latency가 증가하였으나, 혈액과 뇌조직의 anetylcholinesterase 활성에는 영향을 미치지 못하였다. 체내 지질 과산화물은 SAMP8 대조군에서 유의하게 증가하였으나, 탈지 참깨와 탈지 들깨의 메탄올 추출물을 첨가함으로써 유의하게 감소하였으며, SOD와 GPx의 활성은 증가하였다. 따라서 탈지 참깨와 탈지 들깨의 메탄올 추출물은 노화촉진마우스의 인지 기능을 개선시키며, 이는 cholinergic 신경전달체계의 변화보다는 체내 지질 과산화물 억제 및 항산화효소 활성을 통한 메카니즘으로 생각된다.

• 생명과학회지
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• 제9권5호
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• pp.548-555
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• 1999

Long-term effects of Ganoderma lucidum (GL) on memory and oxidative stress of senescence-accelerated mice (SAM) were investigated. Senescence-resistant (R1) and prone (P8) strains of SAM were fed GL diets, premixed with low (20 mg/kg/day, T1) or high (200 mg/kg/day, T2) levels of GL powder for 9 months starting from young (3 months of age) or for 5 months starting from old (7 months of age). After the final feeding at 12 months of age, all animals were subjected to passive avoidance test for the evaluation of memory function. In addition, the changes in hepatic thiobarbituric acid-reactive substance (TBARS) and glutathione were analyzed. SAMP8 fed GL diets from old age (7 months) exhibited the improvement of memory, although GL rather inhibited the memory function of both SAMR1 and SAMP8 mice fed diets from young (3 months of age). Hepatic TBARS contents were decreased in SAMP8 fed high GL diet for 9 months and in SAMR1 fed low GL diet for 5 months. Hepatic glutathione content was also remarkably increased in SAMR1 following both feeding periods, and less extent in SAMP8 fed diet for 5 months of age. Taken together, it is proposed that GL extracts may play an anti-aging role through antioxidant action, and thereby may improve the senescence-related memory dysfunction.

• IMMUNE NETWORK
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• 제5권4호
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• pp.252-257
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• 2005

Background: This study was designed to investigate the effect of exercise training on defense mechanism of chronic degenerative disease, aging, and memory impairments of senescence-accelerated mouse (SAM)P8 under the hypothesis that "Senile dementia may be prevented by regular exercises". Methods: To evaluate the effects of exercise training on the defense mechanism of aging and memory impairment, SAMP8 were divided into two groups, the control group and exercise training groups. the exercise training group were performed with low $(\dot{V}O_2max\;25{\sim}33%)$, middle ($\dot{V}O_2max$ 50%) and high $(\dot{V}O_2max\;66{\sim}75%)$ intensity exercise. All SAMP8 mice were fed experimental diet ad libitum until 4, 8 months, and dead period. Results: Median lifespan in middle exercise group resulted in a significantly increased (23.5% and 18.7%, respectively), whereas these lifespan in high exercise group resulted in an unexpectedly decreased (13.5% and 12.1%, respectively) compared with control group. Body fat levels in 4 and 8 months of age were significantly decreased 43% to 51% in middle exercise group, whereas were remarkably deceased to 57% in high exercise group compared with control group. It is believed that extended median and maximum lifespan may be effected by calory restriction through the exercise training. Acetylcholine (ACh) levels were significantly increased 6.7% and 8.5% in middle and high exercise groups, and also choline acetyltransfease (ChAT) activities were significantly increased 10.3% and 11.9% in middle and high exercise groups. Conclusion: These results suggest that proper and regular exercises such as middle group ($\dot{V}O_2max$ 50%) may play an effective role in attenuating an oxygen radicals and may play an important role in improving a learning and memory impairments of senile dementia.

• Nutritional Sciences
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• 제7권4호
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• pp.189-195
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• 2004

1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided into 2 groups, with thirteen mice each one, as ad libitum group and as dietary restriction group (60% of ad libitum feeding amount). After feeding for a given period, the mice were sacrificed to get the following results: among the experimental groups, there wereno significant differences in xanthine oxidase (XOD) activity in their kidney tissues. The contents of cytochrome $P_{450}$ decreased in ad libitum group and dietary restriction group by age. The activity of NADPH-cytochrome $P_{450}$ reductase showed a trend similar to cytochrome $P_{450}$. Superoxide radical content increased with age. At the 4th, 8th and 12 months of the experimental period, the activity in the dietary restriction group was less than that of ad libitum group by as much as 17% 14% and 14% respectively. For hydrogen peroxide, the contents were increased in the ad libitum group with age, while no correlation between content and age was observed in the dietary restriction group. In the 8th and 12th months of the experimental period, the were in the dietary restriction group less than that of ad libitum group counterpart as much as 17% and 20o/c, respectively. For the cellular membrane stability of the kidney, no significant correlation with age was observed in either the dietary restriction group or the ad libitum group. However at the 12th month of the experiment, however, the stability in the dietary restriction group was 11 % higher than that in the ad libitum group. In conclusion, with these results obtained from the SAMP8 mouse model, we demonstrate that dietary restriction has the effects of anti-oxidation and anti-senescence in the kidney.

• Journal of Photoscience
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• 제9권2호
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• pp.418-420
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• 2002

We measured previously the lipid hydroperoxides level in the brain and peripheral organs such as heart, liver, lung and kidney of senescence acceIerated-prone (SAMP8) and -resistant(SAMR1) mice at 3,6 and 9 months of age. It was found that the lipid hydroperoxide leve1s in the brain did not show any age-dependent change, and that they Were significantly higher in SAMP8 than in SAMR1 over the defined periods. In contrast, the lipid hydroperoxide leve1s in the peripheral organs, including liver, Were increased with aging in both substrain, and they were significantly higher in SAMP8 than in SAMR1 at 3 and 6 months of age. In addition, the lipid hydroperoxide levels in the peripheral organs were higher than those in the brain in both substrains. To elucidate the difference of lipid hydroperoxide levels between the brain and the peripheral organs, we further carried out lipid component analysis in the brain and liver, one of the peripheral organs, of SAMP8 and SAMR1 at 6 months of age.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1253-1258
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• 2012

본 연구에서는 복분자 섭취가 노화에 의한 기억력 감퇴에 개선 효과를 나타내는지 조사하기 위해 노화촉진마우스 (SAM)를 사용하여 평가하였다. 28주령 SAMP8 마우스에 복분자 시료를 100 mg/kg body weight/day로 8주간 투여한 후 수동회피시험과 수중미로시험을 실시하여 기억력 개선효과를 평가하였다. 수동회피시험에서 노화 촉진 동물(SAMP8)은 대조동물(SAMR1)에 비해 밝은 방에서 체류시간이 현저히 감소하였다. 복분자를 섭취한 노화 촉진 동물의 밝은 방에서의 체류 시간은 복분자를 섭취하지 않은 동물에 비해 현저히 증가하였다. 수중미로실험에서 대조 동물에 비해 노화 촉진 동물의 평균 탈출잠복기는 길었고, 복분자를 섭취한 경우 평균 탈출잠복기가 유의적으로 감소하였다. 뇌의 대뇌피질과 해마 조직에서 대조군에 비해 노화 촉진 동물의 acetylcholinesterase 활성이 증가하였다. 복분자를 섭취한 노화 촉진 동물의 해마 조직 내 acetylcholinesterase는 복분자를 섭취하지 않은 동물에 비해 유의적으로 감소하였다. 이 결과들은 복분자가 노화촉진마우스에서 노화에 의한 기억력 감퇴에 대한 개선 효과를 나타내며, 이는 콜린성 신경전달에 중요한 역할을 하는 acetylcholinesterase 활성 감소를 통해 이루어짐을 나타낸다. 향후 더 많은 연구가 수행 되어야 하나, 본 연구는 복분자가 노인의 기억력 개선제 개발에 좋은 기능성 소재로 활용할 수 있음을 제시한다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.90-90
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• 2001

CCAP법을 이용하였을 때 SAM에서 taurine의 뇌 분포용적은 정상생쥐보다 85 % 감소하였다. 그리고 10, 15, 30초 동안의 뇌 분포용적은 linear regression을 나타내었다. 또한 intravenous injection technique의 결과, SAMP8에서의 sucrose space가 정상생쥐에 비하여 감소하였음을 알 수 있었다. 반면에 choline의 경우는 정상생쥐보다 SAMP8의 뇌분포용적이 73% 증가하였다.

• 생명과학회지
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• 제6권2호
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• pp.142-148
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• 1996

Aloe(Aloe arborescens M$_{ILL}$) has been used as a home medicine for the past several thousand in the world, and has been studied on anti-bacterial and anti-fungal activities, hypotension, atherosclerosis, myocardiac infartion, apoplexy, diabetes as a chronic digenerative disease, tumors, gastrointestinal tract, liver and pancreas' diseases, and genitourinary tract etc. SAMP8 as a learing and memory impairment animal model were fed basic and/or experimental diets with 1.0% freezing dried(FD)-aloe for 8 months. The passive avoidance tests such as acqusition trial and retention test were significantly higher in aloe group than in control group. Grading score of senescence resulted in a marked decreases in aloe group compared with control group. Acetylcholinesterase(AChE) activity was remarkably increased in aloe group compared with control group. Neurotransmitters such as dopamine(DA) and serotonin(5-HT) almost did not change by the feeding of aloe-added diet, but their metabolites such as homovanillic acid(HVA) and 5-hydroxy-indole acetic acid(5-HIAA) in aloe group were significantly increased compared with control group. Therefore, the ratios of HVA/DA and 5-HIAA/5-HT as a ratio of metabolite on neurotransmitter were significantly increased by the feeding of aloe-added diet. These results suggest that aloe vara may be activated acetylcholinesterase, the metabolite of neurotransmitter, and ratios of metabolite on neurotransmitter, resulting ina greater prevention of learning and memory impairments such as Alzheimertype dementia.