• Journal of Life Science
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• v.19 no.7
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• pp.886-891
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• 2009

Cancer/testis (CT) antigens are immunogenic in cancer patients, exhibit highly tissue-restricted expression, and are considered promising target molecules for cancer vaccines. We investigated the expression of 13 CT genes in 29 Korean patients with primary breast carcinoma. The most frequently expressed CT genes were MAGE-3 (66%) and MAGE-1 (57%), followed by LAGE-1 (55%), NY-SAR-35 (49%), MAGE-4 (41%), NY-ESO-1 (38%), CT-7 (24%) and SSX-4 (24%), whereas SSX-1, SSX-2, MAGE-10 and NY-TLU-57 were found to be expressed significantly less often (3-7%) and SCP-l not all. Expression of at least one antigen was observed in 28 breast cancer samples. Immunohistochemistry was performed for NY-ESO-l and MAGE-3 protein expression in breast tumor samples. NY-ESO-l and MAGE-3 proteins were expressed in 11 of 29 (38%) and 12 of 29 (41 %) breast tumors. Our results suggest that CT antigens may be potential candidates for polyvalent immunotherapy in Korean breast cancer patients.

• Journal of Sensor Science and Technology
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• v.25 no.1
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• pp.51-56
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• 2016

This study evaluates the performance of an optical sensor and measurement system (CMA-24) which can analyze the fluctuation of dissolved oxygen and pH simultaneously. In the optical sensor system, the fluorescent materials, Rudpp and HPTS which are sensitive to dissolved oxygen and pH, respectively, are coated on the bottom of a 24-well -plate by the sol-gel technology. The detection times of the emission light of the oxygen sensor were $4,186{\pm}13.90{\mu}s$ and $4,452{\pm}36.68{\mu}s$ for the dissolved oxygen of 17% $O_2$ and 7.6% $O_2$, respectively. On the other hand, the detection times of the pH sensor were $6,699.43{\pm}14.64{\mu}s$, $6,722.24{\pm}6.21{\mu}s$, and $6,748.52{\pm}2.63{\mu}s$ using pH 6, 7, and 8, respectively. When we determined cellular respiration levels of C2C12 myocytes with CMA-24, $O_2$/pH measurement system, the ratio of the uncoupled to coupled OCR (oxygen consumption rate) was 1.41. The results mean that this CMA-24 system shows almost the same sensitiveness as the commercial system.

• Journal of the Korean Society of Food Science and Nutrition
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• v.11 no.3
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• pp.57-62
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• 1982

This study was conducted to investigate the changes in plant state, chlorophyll, free amino acids and vitamin C content of soybean sprouts during circulation periods. The experiment was carried out under the indoor natural light and complete darkness at $20^{\circ}C$. The results are summarized as follows; Soybean sprout was grown about one cm in the darkness for 7 or 24 hours, but under the indoor natural light it was not grown. Weight was decreased both in the darkness and under the light. In the darkness the color of sprouts were not changed, but under the light the color was changed in 7 or 24 hours. Chlorophyll contents was increased rapidly by light in the cotyledon. Chlorophyll formation brought about loss not only in amino acids but also porphyrins, but it did not in the amino acids of hypocotyl. The kinds of amino acids being brought about loss under the light were cystine, aspartic acid, serine, histidine and glycine. The 22$\sim$27% of vitamin C was lost in cotyledon by 7 hours exposured light and in hypocotyl after 5 hours.

• Journal of Food Hygiene and Safety
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• v.21 no.1
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• pp.23-30
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• 2006

Antimicrobial susceptibility and multiple drug resistance patterns have been carried out on total of 364 isolates of Salmonella enterica serovar Enteritidis isolated from foodborne patients in Seoul from 2001 to 2005. Overall, the highest percentage of resistance was found to the following antimicrobial agents: streptomycin (46.7%), ampicillin (37.3%), ticarcillin (36.7%), tetracycline (36.0%), nalidixic acid (20.7%), chloramphenicol (13.3%), amoxicillin/clavulanic acid (6.7%) and Ampicillin/sulbactam (4.0%). Seventy five percentage of isolates were found to be resistant to one or more of the antimicrobes tested. The resistant rates to nalidixic acid and chloramphenicol in S. Enteritidis tested were annually increased but the resistant rate to tetracycline was decreased and the resistant rates to streptomycin, ampcilin and ticarcillin were remained steadily. The most frequent patterns of multiresistant isolates were only nalidixic acid resistant (18.0%) and streptomycin-tetracycline (18.0%), streptomycin-ampicillin-ticarcillin (10%), and ampicillin-ticarcillin (5.5%). Overall the resistant rates of 1 drug was 19.3%,2 drugs 24.7%, 3 drugs 6.7% and 4 or more drugs 24.0%. The resistant rates of 1 drug and 2 drugs in 2005 were increased dramatically.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.6B
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• pp.1068-1073
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• 2000

In this paper, we propose a serially concatenated turbo bode/turbo equalizer scheme for optical storage systems. Without modulation coding, a random data sequence is directly passed through the optical channel. In simulation, the channel includes jitter of 15% and AWGN. The densities of the channel are S=4.6 and S=7.0. The code rates of turbo code are 4/5, 8/9 and 16/17. All code rates, the bit error probability is less than 10-5 at 24dB when we and jitter of 15%.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.106-111
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• 1993

Pectolytic enzymes were extracted in apple fruits rotted by Botryosphaeria dothidea, and their activities and change of pectic substances were investigated. Exo-polygalacturonase(exo-PG), exo-polymethylgalacturonase(exe-PMG), polygalacturonate-trans-eliminase(PGTE) and pectin-methyl-trans-eliminase(PMTE) were produced by the pathogen. Activities of exo-PG and exe-PMG extracted from rotten apple fruits were high to 21.15 and 24.65 units/mg protein in specific activity at seven days after inoculation, respectively. Activities of PGTE and PMTE showed 5.60 and 7.90 units/mg protein, respectively, but they were lower than those of the exo-type enzymes. Water-soluble and versene-soluble pectins were 11.50 mg/100 mg-AIS and 7.31 mg/100 mg-AIS at 14 days after inoculation, namely, they were increased by 4.23 and 2.16 mg/100 mg-AIS over those of sound apples, respectively. Total soluble pectic substances of rotten apple were 72.4% of total pectic substances and it was higher by 24.8% than sound apple. Insoluble pectic substance was notably decreased from 15.32 to 7.16 mg/100 mg-AIS according to progress of decay while total pectic substances were not changed remarkably.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.3C
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• pp.234-240
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• 2002

A power amplifier with AlGaAs/InGaAs/GaAs HBT's has been developed for customer premise equipments of the quasi millimeter-wave frequency-band broadband wireless local loop(BWLL) system. Parameters of the linear and nonlinear equivalent circuits for a common base HBT have been extracted by a fitting method. The amplifier has been designed through the linear and nonlinear circuit simulations and fabricated on a ceramic substrate for a hybrid IC. The amplifier has produced a 25.5-dBm output power with 35% power-added efficiency(PAE) at 24.4 GHz and achieved a 7.5-dB linear power gain at 24.8 GHz. In 24.25 ∼24.75 GHz band, the amplifier has exhibited a saturated output over larger than 22 dBm and PAE higher than 25%.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.7 no.4
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• pp.24-36
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• 1997

본 논문에서는 기밀 통신 시스템에서의 지연 분석을 위해 대기 이론ㅇ르 이용하여 시큐리티와 성능사이의 tradeoff를 정량화하고, 시큐리티 메커니즘과 프로토콜에 의해 야기된 지연을 줄이기 위한 최적화기법으로 전처리, 메시지의 분할과 압축, 압축과 암호의 통합, 사용자 인증과 접근 제어의 통합을 고려한다. 그리고, 시큐리티 서비스를 제공하기 위한 시큐리티 메커니즘으로 DES를 이용한 경우, RAS 의 디지털 서명, IDEA와 RSA의 결합을 고려하고, 각각에 대해 대개 이론의 M/M/1모델, M/E/1모델, M/H/1 모델을 적용하여 컴퓨터 시뮬레이션을 통해 평균 지연을 분석한다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.7
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• pp.930-937
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• 2000

A series of data sets for the heat transfer coefficient versus wall superheat has been obtained experimentally using various combinations of tube diameters ($9.7{\sim}25.5mm$), surface roughness ($15.1{\sim}60.9nm$), and tube orientations (horizontal and vertical) to obtain effects of tube diameters on nucleate pool boiling heat transfer for the saturated water at atmospheric pressure. In addition, the results are compared with the well known Cornwell and Houston's correlation for horizontal tubes to identify the deviation of the present experimental data from the correlation and the applicability of it to vertical tubes. The experimental results show that the heat transfer coefficient decreases as the tube diameter increases for both horizontal and vertical tubes and they are in good agreement with the Cornwell and Houston's correlation within ${\pm}20%$ scatter range.

• Molecules and Cells
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• v.27 no.5
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• pp.601-608
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• 2009

The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK, and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans SK-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 $[M+H]^+$, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of metabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of $324[M+H]^+$. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.