• 제목/요약/키워드: S20C

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갈조류 개다시마 유리배우체의 재생 및 성숙 유도 (Regeneration and the Maturation Induction of Free-Living Gametophytes of a Kelp Saccharina sculpera (Phaeophyceae))

  • 유현일;이기현;김수홍;하동수;황은경
    • 환경생물
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    • 제36권4호
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    • pp.576-583
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    • 2018
  • 본 연구에서는 다양한 온도(5, 10, 15, 20, $25^{\circ}C$), 조도(10, 20, 40, 60, $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$) 및 광주기(10:14, 12:12, 14:10 h L:D) 조건에서 개다시마 유리배우체 단편의 생장 및 성숙 유도조건을 파악하였다. 암배우체 단편의 생장은 $20^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D) 조건에서 빠른 생장을 보인 반면 수배우체는 $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) 조건에서 빠른 생장을 보여 차이를 나타내었다. 암배우체의 최적 성숙조건은 $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) 조건에서 가장 빠른 성숙을 보인 반면 수배우체는 $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D) 조건에서 빠른 성숙을 보여 차이를 나타내었다. 본 연구 결과 확인된 개다시마 유리배우체 단편의 생장 및 성숙조건은 개다시마의 자원조성 및 대량양식 기반조성의 기초자료로 활용될 수 있다.

미래적 이미지 패션의 연구 (A Study on Futuristic Image of Fashion - Futuristic Image in 20C Fashion -)

  • 유현정
    • 한국의상디자인학회지
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    • 제14권2호
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    • pp.11-21
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    • 2012
  • The purpose of this study is to understand of future image in fashion for 20C, to suggest a guideline's role in the development on fashion designs study. The methods of this study are used academic literatures as well as practical study. Futuristic image in fashion is to introduce about the future period, as fashion goes ahead times. Trends and formative characteristics of future image in 20C fashion are summarized as follows. First, futuristic images in fashion for 20C had emerged strongly futurism, space look, kinetic look, glitter look, techno look, cyber look. Second, perspectives on response for future society's changes are summarized as follows. Attitude of futuristic images of fashion, futurism, space look, kinetic look and glitter look, had been favorable about future society's changes until 1980. In 1990, techno look had been defensive and cyber look had been aggressive about future society's changes. Third, formative characteristics of future image in 20C fashion are studied shape, material, color, pattern. Shape was preferred linear and geometric form in the first half of the 20C, but body conscious type had appeared often in the second half of 20C. Material maintained the luster or the metal touch. Color was based image of colorless and preferred the gold, silver or color feels like metal. Pattern was preferred clarity with geometric type in the first half of the 20C. On the other hand in the second half of 20C pattern of future image was dominated by vague and abstract chaotic type.

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빵가루 입힌 냉동새우의 동결저장중 온도변동에 의한 품질변화 (Change in Quality of Frozen Breaded Raw Shrimp by Storage Temperature Fluctuation)

  • 정진웅;조진호;임상동;강통삼
    • 한국식품과학회지
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    • 제23권5호
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    • pp.532-537
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    • 1991
  • 빵가루 입힌 냉동새우에 있어서 동결저장중 온도변동이 품질에 미치는 영향을 휘발성염기질소, pH, 염용성 단백질 용출량, TBA값과 미생물학적 변화에 의해 조사하였다. 저장온도를 $-20^{\circ}C$에서 20일간 또는 40일간 저장 후 $-15^{\circ}C$$-5^{\circ}C$까지 온도를 상승시켜 그대로 저장한 경우, $-15^{\circ}C$까지의 온도상승시 품질변화는 $-20^{\circ}C$ 저장시와 거의 차이가 없으나 $-5^{\circ}C$까지 상승시킨 경우에는 상승 전의 저장기간 차에 상관없이 VBN, pH, TBA 및 염용성단백질의 용출량과 미생물학적 변화가 현저함을 볼 수 있었다. 그리고 품온을 $-20^{\circ}C$에서 $-15^{\circ}C$까지의 상하변동을 되풀이 한 경우, 변동횟수에 관계없이 초기치의 품질지표값과 거의 차가 없는 반면에, 품온을 $-20^{\circ}C$에서 $-10^{\circ}C$$-5^{\circ}C$까지의 온도 상하변동을 되풀이하여 실시한 경우에는 시료의 VAN, pH, TBA, 염용성단백질 용출량 및 미생물학적 변화는 온도폭이 큰 $-5^{\circ}C$까지의 변동에서 보다 큰 품질변화를 보여주었으며 이는 변동횟수의 증가에 따라 더욱 증가되는 현상으로 나타났다.

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Cloning and Regulation of Schizosaccharomyces pombe Gene Encoding Ribosomal Protein S20

  • Lee, Yoon-Jong;Kim, Kyunghoon;Park, Eun-Hee;Ahn, Ki-Sup;Kim, Daemyung;Lim, Chang-Jin
    • Journal of Microbiology
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    • 제39권1호
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    • pp.31-36
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    • 2001
  • A cDNA clone encoding the ribosomal protein S20 has been isolated from the Schizosaccharomyces pombe cDNA library by colony hybridization. The insert contained in the original plasmid pYJ10 was transferred intro shuttle vector pRS316 generate plasmid pYJll. The dDNA insert of plasmid pYJll, contains 484 nucleotides and encodes a protein of 118 amino acids with a calculated mass of 13,544 daltons. The deduced amino acid sequence of S. pombe ribosomal protein S20 is very homologous with fruit fly, rat, and budding yeast counterparts. It is also homologous with Xenopus S22 ribosomal protein. S. pombe ribosomal protein S20 appears to be relatively hydruphobic except the C-terminal region. The 728 bp upstream region of the S20 gene was amplified from chromosomal DNA and transferred into the BamHI/EcoRI site of the promoterles $\beta$-galactosidase gene of the vector YEp357R, which resulted in fusion plasmid pYS20. The synthesis of $\beta$-galactosidase from the fusion plasmid appeared to be the highest in the mid-exponential phase. The S. pombe cells with the fusion plasmid grown at 35$\^{C}$ gave lower $\beta$-galactosidase activity than the cells grown at 30$\^{C}$. Computer analysis showed the consensus sequence CAGTCACA in the upstream regions of various ribosomal protein genes in S. pombe, which would be involved in the coordinated expression of small ribosomal proteins.

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인삼의 Protopanaxadiol계 사포닌으로부터 20(R)-Ginsenoside $Rh_2$ 및 20(S) 이성체의 제조 (Preparation of a 20(R)-Ginsenoside $Rh_2$ and the 20(S) Epimer from Protopanaxadiol Saponins of Panax ginseng C.A. Meyer)

  • 김신일;백남인;김동선;이유희;강규상;박종대
    • 약학회지
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    • 제35권5호
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    • pp.432-437
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    • 1991
  • A mixture of 20(R)- and 20(S)-ginsenoside Rg$_{3}$ was obtained under mild acidic hydrolysis from protopanaxadiol saponins, ginsenosides Rb$_{1}$, Rb$_{2}$, Rc and Rd. The product was acetylated to give the peracetates, which were further converted into 20(R)-ginsenoside Rg$_{3}$, 20(S)-ginsenoside Rg$_{3}$, 20(R)-ginsenoside Rh$_{2}$ and 20(S)-ginsenoside Rh$_{2}$ by the direct alkaline treatment depending upon two kinds of temperature conditions respectively. The structure and physicochemical properties of a prosapogenin, 20(R)-ginsenoside Rh$_{2}$, were investigated.

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Complete $^1H$-NMR and $^{13}C$-NMR spectral analysis of the pairs of 20(S) and 20(R) ginsenosides

  • Yang, Heejung;Kim, Jeom Yong;Kim, Sun Ok;Yoo, Young Hyo;Sung, Sang Hyun
    • Journal of Ginseng Research
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    • 제38권3호
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    • pp.194-202
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    • 2014
  • Background: Ginsenosides, the major ingredients of Panax ginseng, have been studied for many decades in Asian countries as a result of their wide range of pharmacological properties. The less polar ginsenosides, with one or two sugar residues, are not present in nature and are produced during manufacturing processes by methods such as heating, steaming, acid hydrolysis, and enzyme reactions. $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the identification of the less polar ginsenosides are often unavailable or incomplete. Methods: We isolated 21 compounds, including 10 pairs of 20(S) and 20(R) less polar ginsenosides (1-20), and an oleanane-type triterpene (21) from a processed ginseng preparation and obtained complete $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the following compounds, referred to as compounds 1-21 for rapid identification: 20(S)-ginsenosides Rh2 (1), 20(R)-Rh2 (2), 20(S)-Rg3 (3), 20(R)-Rg3 (4), 6'-O-acetyl-20(S)-Rh2 [20(S)-AcetylRh2] (5), 20(R)-AcetylRh2 (6), 25-hydroxy-20(S)-Rh2 (7), 25-hydroxy-20(S)-Rh2 (8), 20(S)-Rh1 (9), 20(R)-Rh1 (10), 20(S)-Rg2 (11), 20(R)-Rg2 (12), 25-hydroxy-20(S)-Rh1 (13), 25-hydroxy-20(R)-Rh1 (14), 20(S)-AcetylRg2 (15), 20(R)-AcetylRg2 (16), Rh4 (17), Rg5 (18), Rk1 (19), 25-hydroxy-Rh4 (20), and oleanolic acid 28-O-b-D-glucopyranoside (21).

Effect of Ginseng Saponin on Hypothalamus-Pituitary- Adrenal Axis under Stress in Mice

  • Do Hoon Kim;Jun
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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    • pp.83-89
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    • 1998
  • Ginseng total saponins (GTS) injected intracerebroventricularly (i.c.v.) at doses from 0.1-1 vs inhibited the i.c.v. injection stress-induced plasma corticosterone levels in mice. The inhibitory action of GTS was blocked by co-administered NG-nitro-L-arginine methyl ester (L-NAME; 1.5 us, i.c.v.), an. inhibitor of nitric oxide synthase (NOS). Of the ginsenosides Rbl, Rba, Rc, Rd, Re, Rf, Rgl,20(S)-Rg3, and 20(R)-Rg3 injected i.c.v. at doses from 0.01 to 0.3ug(or 1 uE),20(5)-Rg3 and Rc significantly inhibited the o.c.v. injection stress-induced Plasma corticosterone levels. The inhibitory actions of 20(S)-Rg3 and Rc were blocked by co-administered L-NAME (1.5 n, i.c.v.). These results suggest that G75, 20(S)-Rg3 and Rc may inhibit the i.c.v. injection stress-induced hypothalamo-pituitary-adrenal response by inducing NO production in the brain.

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M68020 C CROSS COMPILER SYSTEM의 3B20S에의 이식 (PORTING OF M68020 C CROSS COMPILER SYSTEM ONTO 3B20S COMPUTER)

  • 김완태;정상현;최영철;유근호;여재흥
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1988년도 전기.전자공학 학술대회 논문집
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    • pp.644-646
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    • 1988
  • This paper has been aimed to develop M68020 Software Development System on a host computer 3B20S for the TDX-2 fully electronic Switching system by transporting M68020 C Cross Compiler. M68020 C Cross Compiler source code which includes assembler, run-time library and optimizer has been analyzed for the installation on the host computer 3B20S system. Moreover, the linkage editor source file has been analyzed and installed on the3B20S to produce the executable file correctly. Through these procedures, the M68020 object codes could be obtained on the 3B20S computer for the multi-using purposes. It has also been confirmed that the M68020 Software Development System on the 3B20S works correctly.

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굴참나무와 사과나무로부터 제조한 훈연액의 제조온도에 따른 돌연변이원성에 관한 연구 (Mutagenic Activity of Smoke Flavoring Processed from Oak and Apple Wood on Manufacturing Temperature)

  • 강희곤;이경호;홍희선;박상진;김창한
    • 한국축산식품학회지
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    • 제18권3호
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    • pp.203-208
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    • 1998
  • The study was carried out to screen mutagenicity of smoking materials for the determination of optimum smoking temperature for meat products. Wood materials employed for smoking were oak and apple trees. Temperatures of the generator for manufacturing of smoke flavoring were set to 250$^{\circ}C$, 400$^{\circ}C$ and 500$^{\circ}C$, respectively. Mutagenic activities of smoke flavoring were assayed according to Ames test using Salmonella typhimurium TA98 and TA 100. In oak wood smoke flavoring, Salmonella typhimurium TA98 without S-9 mix showed strong mutagenic activities at the concentration of 6$\mu\textrm{g}$/plate(250$^{\circ}C$), 4$\mu\textrm{g}$/plate(400$^{circ}C$) and 6$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA100 with S-9 mix showed strong mutagenic activities at the concentration of 10$\mu\textrm{g}$/plate(250$^{\circ}C$), 20$\mu\textrm{g}$/plate(400$^{\circ}C$) and 10$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA98 with S-9 mix showed strong mutagenic activities at the concentration of 30$\mu\textrm{g}$/plate(250$^{\circ}C$), 40$\mu\textrm{g}$/plate(400$^{\circ}C$) and 20$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA100 with S-9 mix showed strong mutagenic activities at the concentration of 30$\mu\textrm{g}$/plate(250$^{\circ}C$), 50$\mu\textrm{g}$/plate(400$^{\circ}C$) and 20$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA100 without S-9 mix showed strong mutagenic activities at the concentration of 10$\mu\textrm{g}$/plate(250$^{\circ}C$), 20$\mu\textrm{g}$/plate(400$^{\circ}C$) and 20$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA98 with S-9 mix showed strong mutagenic activities at the concentration of 30$\mu\textrm{g}$/plate(250$^{\circ}C$), 40$\mu\textrm{g}$/plate(400$^{\circ}C$) and30$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA100 with S-9 mix showed strong mutagenic activities at the concentrations 30$\mu\textrm{g}$/plate(500$^{\circ}C$). Salmonella typhimurium TA100 with S-9 mix showed strong mutagenic activities at the concentration of 30$\mu\textrm{g}$/plate(250$^{\circ}C$), 20$\mu\textrm{g}$/plate(400$^{\circ}C$) and 30$\mu\textrm{g}$/plate(500$^{\circ}C$). From these results, it could be concluded that optimum smoking temperature for meat products should be set below 400$^{\circ}C$, that the compounds like benzo[a]pyrene etc. contain a variety of mutagenic potentials, which could be generated at the higher smoking temperature.

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