Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Regeneration and the Maturation Induction of Free-Living Gametophytes of a Kelp Saccharina sculpera (Phaeophyceae)

갈조류 개다시마 유리배우체의 재생 및 성숙 유도

  • Yoo, Hyun Il (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Lee, Ki Hyun (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Kim, Soo Hong (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Ha, Dong Soo (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Hwang, Eun Kyoung (Seaweed Research Center, National Institute of Fisheries Science)
  • 유현일 (국립수산과학원 해조류연구센터) ;
  • 이기현 (국립수산과학원 해조류연구센터) ;
  • 김수홍 (국립수산과학원 해조류연구센터) ;
  • 하동수 (국립수산과학원 해조류연구센터) ;
  • 황은경 (국립수산과학원 해조류연구센터)
  • Received : 2018.11.22
  • Accepted : 2018.12.06
  • Published : 2018.12.31
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Abstract

Investigation of optimal temperature, irradiance and photoperiod conditions for free-living gametophytes of Saccharina sculpera for natural resources conservation and mass cultivation of endangered species in the eastern coast of Korea. Induction of regeneration and maturation of the free-living gametophytes of S. sculpera were cultured at temperatures (5, 10, 15, 20 and $25^{\circ}C$), irradiance (10, 20, 40, 60 and $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and photoperiods (14:10, 12:12 and 10:14 h L:D). The female gametophyte were actively regenerated without reaching sexual maturity under $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D) conditions. In contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $10{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D). The sexual maturation of female and male gametophytes took place under $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) and $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for conservation and utilization of S. sculpera.

본 연구에서는 다양한 온도(5, 10, 15, 20, $25^{\circ}C$), 조도(10, 20, 40, 60, $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$) 및 광주기(10:14, 12:12, 14:10 h L:D) 조건에서 개다시마 유리배우체 단편의 생장 및 성숙 유도조건을 파악하였다. 암배우체 단편의 생장은 $20^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D) 조건에서 빠른 생장을 보인 반면 수배우체는 $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) 조건에서 빠른 생장을 보여 차이를 나타내었다. 암배우체의 최적 성숙조건은 $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) 조건에서 가장 빠른 성숙을 보인 반면 수배우체는 $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D) 조건에서 빠른 성숙을 보여 차이를 나타내었다. 본 연구 결과 확인된 개다시마 유리배우체 단편의 생장 및 성숙조건은 개다시마의 자원조성 및 대량양식 기반조성의 기초자료로 활용될 수 있다.

Keywords

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