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Adherence-induced gene expression in human alveolar macrophages (표면부착에 의한 사람 폐포대식세포의 유전자 발현에 관한 연구)

  • Chung, Man Pyo;Yoo, Chul Gyu;Han, Sung Koo;Shim, Young-Soo;Rhee, Chong H.;Han, Yang Chol;Kim, Young Whan
    • Tuberculosis and Respiratory Diseases
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    • v.43 no.6
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    • pp.936-944
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    • 1996
  • Background: Neutrophils or monocytes separated in vitro by the adherence to plastic surface are known to be activated by surface adherence itself and subsequent experimental data might be altered by surface adherence. Adhesion molecules and gene transcription of the inflammatory mediators are known to be associated in this process. To evaluate whether adhesion molecule and transcriptional activation of the inflammatory substances are also involved in the activation of human alveolar macrophage by the adherence procedure, we designed this experiment. Method : Bronchoalveolar lavage was performed in the person whose lung of either side was confirmed to be nonnal by chest cr and alveolar macrophage was harvested. To measure the expression of Interleukin-8(IL-8) mRNA, manganese superoxide dismutase(SOD) mRNA and CD11/CD18 mRNA in human alveolar macrophage of both adherence state and suspension state, Northern blot analysis was done at 0, 2, 4, 8 and 24hrs after the adherence to plastic surface and during suspension state. Then, phorbol myristate acetate(pMA) and N-formyl-methionyl-leucyl-phenylalanine(fMLP) were added respectively in the same experimental condition. Result : 1) Human alveolar macrophages in the adherent state induced IL-8 mRNA and SOD mRNA expression which was maximal at 8 hours after the adherence to plastic surface. But we could not observe the upregulation of CD18 mRNA by surface adherence. 2) PMA induced these mRNA expression both in the adherent cell and the nonadherem cells, but the induction of mRNA expression by fMLP occurred only in the adherent cells. Conclusion: These results suggest that adherence of huamn alveolar macropahge is an important cell-activating event that may play a critical role in the modulation of lung inflammatory respones.

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Evaluation of Denitrification Reactivity by the Supported Nanoscale Zero-Valent Iron Prepared in Ethanol-Water Solution (이중용매에서 제조된 나노영가철을 이용한 질산성질소의 환원반응성 평가)

  • Park, Heesu;Park, Yong-Min;Oh, Soo-Kyeong;Lee, Seong-Jae;Choi, Yong-Su;Lee, Sang-Hyup
    • Korean Chemical Engineering Research
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    • v.46 no.5
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    • pp.1008-1012
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    • 2008
  • Nanoscale zero-valent iron(nZVI) is famous for its high reactivity originated from its high surface area and it has received considerable attentions as one of the latest innovative technologies for treating contaminated groundwater. Due to its fine powdery form, nZVI has limited filed applications. The efforts to overcome this shortcoming by immobilizing nZVI on a supporting material have been made. This study investigated the differences of resin-supported nZVI's characteristics by changing the preparation methods and evaluated its reactivity. The borohydride reduction of an iron salt was proceeded in ethanol/water solvent containing a dispersant and the synthesis was conducted in the presence of ion-exchange resin. The resulting material was compared to that prepared in a conventional way of using de-ionized water by measuring the phyrical and chemical characteristics. BET surface area and Fe content of nZVI-attached resin was increased from $31.63m^2/g$ and 18.19 mg Fe/g to $38.10m^2/g$ and 22.44 mg Fe/g, respectively, by switching the solution medium from water to ethanol/water with a dispersant. The reactivity of each material was tested using nitrate solution without pH control. The pseudo first-order constant of $0.462h^{-1}$ suggested the reactivity of resin-supported nZVI prepared in ethanol/water was increased 61 % compared to that of the conventional type of supported nZVI. The specific reaction rate constant based on surface area was also increased. The results suggest that this new supported nZVI can be used successfully in on-site remediation for contaminated groundwater.

A study on the Effects of Rehmannia Radix and Eukmigihwangtang (EMGHT) on Antioxidation Activity in Aging Rats (숙지황(熟地黃)과 육미지황탕(六味地黃湯)이 노화과정(老化過程) 흰쥐에서의 항산화(抗酸化) 기전(機轉)에 미치는 영향(影響))

  • Ahn, Sang-Won;Lee, Cheol-wan
    • Journal of Haehwa Medicine
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    • v.8 no.1
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    • pp.593-623
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    • 1999
  • This experimental study was designed to verify the anti-aging efficacy of Eukmigihwangtang (EMGHT) and Rehmannia Radix, and determine the specific role and actions of Rehmannia Radix. Normal rat (2 months old), aging rat (8 months old), and pathologically induced rat (2 months old, injected 30mg/kg of streptozotocin) are observed to study the aging eliciting factors such as peroxide contents and enzyme activities. The following results were obtained in this study: 1. For the body weight changes, normal group given Rehmannia Radix showed decrease in the body weight compared to the control group, aging group given EMGHT and Rehmannia Radix showed significant decrease in the body weight, and STZ injected group showed suppression to the body weight loss when given EMGHT and Rehmannia Radix. 2. For the content changes in serum lipid peroxide, normal group showed increasing level as the rat gets older. Aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant decrease in the lipid peroxide level compared to the control group. Decrease was more prominant in the group given EMGHT. 3. For the changes in serum hydroxyl radical, normal group did not show significant changes, but aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant decrease in the hydroxyl radical level compared to the control group. Decrease was more prominant in the group given EMGHT. 4. For the changes in serum superoxide dismutase (SOD) activity, normal group did not show significant changes, but aging group given EMGHT and Rehmannia Radix showed significant increase in the SOD activity compared to the control group. STZ injected group given EMGHT and Rehmannia Radix showed significant decrease in the SOD activity compared to the control group. 5. For the content changes in hepatic lipid peroxide, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant decrease in the lipid peroxide level compared to the control group. 6. For the changes in hepatic cytochrome P-450 activity, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant decrease compared to the control group. Cytochrome b5 activity was significantly decreased only in the STZ injected group given EMGHT and Rehmannia Radix. 7. For the changes in hepatic aminopyrine demethylase and aniline hydroxylase activity, aging group given EMGHT and Rehmannia Radix showed significant decrease compared to the control group. STZ injected group given EMGHT and Rehmannia Radix showed significant increase in the aminopyrine demethylase activity, and showed significant decrease in the aniline hydroxylase activity compared to the control group. 8. For the content changes in hepatic protein bound-SH and nonprotein bound-SH, againg group and STZ injected group given EMGHT and Rehmannia Radix showed significant increase compared to the control group. 9. For the content changes in hepatic glutathione level, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant increase compared to the control group. 10. For the changes in hepatic glutathione S-transferase activity, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant increase and decrease, respectively, compared to the control group. 11. For the changes in hepatic glutathione reductase activity, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant increase compared to the control group, while $\gamma$-Glutamylcystein synthetase activity did not show significant changes. 12. For the changes in hepatic superoxide dismutase activity, aging group and STZ injected group given EMGHT and Rehmannia Radix showed significant decrease compared to the control group. From the above results, the antioxidant effects of EMGHT and Rehmannia Radix were proved, as well as the role of Rehmannia Radix, a chief of EMGHT, was examined. In addition, since no change was reconized as the quantity of Rehmannia Radix and the order herbs increased, the reasonableness on EMGHT was proven with respect to its composition and quantity. Thus, the significance of EMGHT could be objectively exmined in terms of its composition and quantity. Considering animals used in the experiment, there were obvious changes in aging rats and pathologically induced rats than in normal rats. Consequently, it was noticeable that EMGHT and Rehmannia Radix were working selectively on the subjects.

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The Effect of Packing Method of Relining Material on the Flexural Strength of Denture Base Resin (첨상용 레진의 성형법이 의치상의 굴곡강도에 미치는 영향)

  • Kim, Min-Chul;Kim, Yu-Lee
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.2
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    • pp.197-207
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    • 2011
  • The study aimed at examining how different reline resins affect flexural strength and flexural modulus of denture base. A total of 80 specimens ($64{\times}10{\times}3.3$ mm, according to ISO 1567:1999) of heat-polymerized resin, 40 specimens for (Lucitone199(Dentsply Int., NewYork, USA), SR Ivocap(Ivoclar AG, Schaan, Liechtenstein)) respectively, were polymerized according to the manufacturer's instructions and divided into eight groups(n = 10). Control group specimens remained intact. Specimens in the other groups were abraded on both sides to 2 mm thickness, and were relined in 1.3 mm thickness with 3 types of resins (Lucitone199(Dentsply), SR Ivocap(Ivoclar), and Rebase II(Tokuyama Co., Ltd, Tokyo, Japan)). All specimens were preserved in distilled water at $37^{\circ}C$ for 50 hours, and then were subjected to flexural strength testing in a universal testing machine using 3-point loading. A crosshead speed of 5 mm/min was used, and the distance between the supports was 50 mm. Data analyses included one-way analysis of variance(ANOVA) and the Tukey Honestly Significant Difference test (p=.05). Both heat-polymerized resin groups and auto-polymerized resin groups showed statistically low flexural strength and flexural modulus than control groups. Specimens relined with Lucitone 199 showed significantly higher flexural strength and flexural modulus than those relined with SR-Ivocap. Specimens relined with auto-polymerized resin showed significantly lower flexural strength and flexural modulus than those relined with heat-polymerized resin. Relining with heat-polymerized resins showed superior mechanical properties to relining with an auto-polymerized resin. Relining with the same heat-polymerized resin as the denture base does not affect mechanical properties of a denture. Lucitone199 using a compression-mould technique resulted in the highest flexural strength.

Legibility Evaluation of Two and Three Syllable Words Used in Pesticides According to Font, Thickness, Gender, and Visual Acuity (시력, 폰트, 굵기, 성별에 따른 2음절 및 3음절 농약 제품 표시글자의 가독성 평가)

  • Hwang, Hae-Young;Song, Young-Woong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.8
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    • pp.3444-3451
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    • 2012
  • Safety and health related information for the proper use and handing of pesticides is usually printed on the surface of the pesticide products in the form of texts. But, the guidelines or standards for the appropriate presentation of the texts for the pesticide products are most vague or not practical. Thus, this study aimed to provide the preliminary guidelines for the text sizes based on the legibility experiments. To achieve the objective legibility evaluation experiments were conducted to test the effects of different near vision (0.6, ${\geq}0.8$), gender, font type(thick gothic-type and fine gothic-type), thickness of font(plain and bold), and number of syllables(2 and 3 syllables) in the same age group of 20s. The results showed that legibility was different according to the visual acuity (p<0.05), and no other main effects showed statistically significant effects. The 'maximum illegible size' to read at least one word correctly in all the text conditions was 2 pt when the near vision was ${\geq}0.8$, and 2 pt or 3 pt when the near vision was 0.6. The 'minimum legible size' for 100% correct answer was 9 pt for the near vision of 0.6, and 5.3 pt for ${\geq}0.8$, respectively. Mean character size does not read any discomfort in 0.6 was 15.5 pt in both male and female but male was mean 8.5 pt, female was 10 pt in ${\geq}0.8$. Considering these experimental results, it was recommended that the 16 pt or larger characters should be used the important information such as 'Pesticides' or toxicity, and the minimum character size was 9 pt for the less important information.

Humidification and Shading Affect Growth and Development of Cutting Propagated 'Maehyang' Strawberry (Fragaria × ananassa Duch.) at Propagation Stage (삽목번식 시 가습과 차광 처리에 따른 '매향' 딸기의 생육)

  • Kang, Dong Il;Jeong, Hai Kyoung;Park, Yoo Gyeong;Wei, Hao;Hu, Jiangtao;Jeong, Byoung Ryong
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.429-437
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    • 2019
  • This study was conducted to examine the effect of humidification and shading during cutting propagation on growth and development of strawberry (Fragaria x ananassa Duch.) 'Maehyang' plants at a propagation stage. The runner cuttings were stuck on Nov. 23, 2017 in propagation benches set in a Venlo-type glasshouse. Four shading treatments, no shading (control, C), 55% shading with white lawn (W55), 55% black shading net (B55), or 100% black plastic film (B100) with either an intermittent fog system (H) or without fog system. The shading and fog systems were removed 2 weeks after sticking of strawberry cuttings. A nutrient solution for strawberry, which was developed by Yamazaki, was supplied once a day with electrical conductivity (EC) $1.6dS{\cdot}m^{-1}$ and pH 5.8. Growth parameters such as plant height, longest root, crown diameter, leaf chlorophyll, leaf area and fresh and dry weight were measured at 7 days and 26 days after sticking. There was no significant difference in growth of above-aerial part of strawberry. The overall growth of the strawberry roots was better grew by providing fog than that not provide fog. The root fresh weight and root dry weight after 26 days after sticking of strawberry cutting was the best in the treatment that provided fog system without shading (CH). The longest root after 26 days after sticking of strawberry cutting was the best in the treatments that provided fog system with either 55% white lawn (W55H) and 55% black shading net (B55H). These results suggest that morphogenesis of these plants were affected by humidification and shading types. In a broader perspective, these results can be used to optimize studies of other crops grown from cuttings.

Disappearance of Hysteria(Conversion Disorder) and the Evolutionary Brain Discord Reaction Theory (히스테리아(전환장애)의 소실과 진화적 뇌신경 부조화 반응 가설)

  • Song, Ji Young
    • Korean Journal of Psychosomatic Medicine
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    • v.24 no.1
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    • pp.28-42
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    • 2016
  • Objectives : The author tried to find out reasons why and how hysteria(and conversion disorder) patient numbers, which were so prevalent even a few decades ago, have decreased and the phenotype of symptoms have changed. Methods : The number of visiting patients diagnosed with conversion disorder and their phenotype of symptoms were investigated through chart reviews in a psychiatric department of a University hospital for the last 12 years. Additionally, the characteristics of conversion disorder patients visiting the emergency room for last 2 years were also reviewed. Those results were compared with previous research results even if it seemed to be an indirect comparisons. The research relied on Briquet P. and Charcot JM's established factors of the vicissitudes of hysteria(and conversion disorder) which has been the framework for more than one hundred and fifty years since hysteria has been investigated. Results : The author found decreased numbers and changes of the phenotype of the hysteria patients(and conversion disorder) over the last several decades. The decreased numbers and changes of the symptoms of those seemed to be partly due to several issues. These issues include the development of the diagnostic techniques to identify organic causes of hysteria, repeated changes to the symptom descriptions and diagnostic classification, changes of the brain nervous functions in response to negative emotions, and the influence of human evolution. Conclusions : The author proposed that the evolutionary brain discord reaction theory explains the causes of disappearance of and changes to symptoms of hysteria(conversion disorder). Most patients with hysteria(conversion disorder) have been diagnosed in the neurological department. For providing more appropriate treatment and minimizing physical disabilities to those patients, psychiatrists should have a major role in cooperating not only with primary care physicians but with neurologists. The term 'hysteria' which had been used long ago should be revived and used as a term to describe diseases such as somatic symptom disorder, functional neurological symptoms, somatization, and somatoform disorders, all of which represent almost the same vague concept as hysteria.

A Study on the Asia Container Ports Clustering Using Hierarchical Clustering(Single, Complete, Average, Centroid Linkages) Methods with Empirical Verification of Clustering Using the Silhouette Method and the Second Stage(Type II) Cross-Efficiency Matrix Clustering Model (계층적 군집분석(최단, 최장, 평균, 중앙연결)방법에 의한 아시아 컨테이너 항만의 클러스터링 측정 및 실루엣방법과 2단계(Type II) 교차효율성 메트릭스 군집모형을 이용한 실증적 검증에 관한 연구)

  • Park, Ro-Kyung
    • Journal of Korea Port Economic Association
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    • v.37 no.1
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    • pp.31-70
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    • 2021
  • The purpose of this paper is to measure the clustering change and analyze empirical results, and choose the clustering ports for Busan, Incheon, and Gwangyang ports by using Hierarchical clustering(single, complete, average, and centroid), Silhouette, and 2SCE[the Second Stage(Type II) cross-efficiency] matrix clustering models on Asian container ports over the period 2009-2018. The models have chosen number of cranes, depth, birth length, and total area as inputs and container TEU as output. The main empirical results are as follows. First, ranking order according to the efficiency increasing ratio during the 10 years analysis shows Silhouette(0.4052 up), Hierarchical clustering(0.3097 up), and 2SCE(0.1057 up). Second, according to empirical verification of the Silhouette and 2SCE models, 3 Korean ports should be clustered with ports like Busan Port[ Dubai, Hong Kong, and Tanjung Priok], and Incheon Port and Gwangyang Port are required to cluster with most ports. Third, in terms of the ASEAN, it would be good to cluster like Busan (Singapore), Incheon Port (Tanjung Priok, Tanjung Perak, Manila, Tanjung Pelpas, Leam Chanbang, and Bangkok), and Gwangyang Port(Tanjung Priok, Tanjung Perak, Port Kang, Tanjung Pelpas, Leam Chanbang, and Bangkok). Third, Wilcoxon's signed-ranks test of models shows that all P values are significant at an average level of 0.852. It means that the average efficiency figures and ranking orders of the models are matched each other. The policy implication is that port policy makers and port operation managers should select benchmarking ports by introducing the models used in this study into the clustering of ports, compare and analyze the port development and operation plans of their ports, and introduce and implement the parts which required benchmarking quickly.

Growth Conditions and Maintenance of Natural Monument Old Big Trees in Gangwondo (강원도 내 천연기념물 노거수의 생육환경 및 관리현황)

  • Heo, Bok Soo;Kim, Hyo Jeong;Lee, Won Ho;Kang, Hee Jin
    • Korean Journal of Heritage: History & Science
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    • v.44 no.1
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    • pp.182-195
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    • 2011
  • Old big tree transcends the simple meaning of trees as they are the natural monuments that embody the people's history and culture of this land. The Cultural Heritage Administration of Korea (CHA) defines and protects old big tree based not only on the size of the tree but also on its definitive cultural and natural factors such as value, implications, and originality. This research aims to identify and analyze the habitation and measurements, the soil conditions, as well as the protective facilities of 14 old big tree spread throughout the Gangwondo. The research examined the soundness of the arboreal form, the degree of damage on the bark, as well as the quantity of leafs levels to evaluate the overall condition of growth and development. Then, the tree height, the eastwest and south-north width of the crown, the sternum circumference, and the root circumference were recorded to identify the measurements of the selected old big tree. Also, the research analyzed 9 elements such as soil texture, pH levels, and organic contents in order to determine the scientific characteristics of soil upon which the trees draw nutrition. Finally, the research evaluated supporting pillars, lightning rods, iron pulling-facilities, as well as notice boards to study the protective facilities of old big tree. The research procedures revealed that 78.57% of the old big tree identified as natural monuments in the Gangwondo have average to above average habitation conditions. Also, the average tree height reaches 18.80m above ground while the average root circumference measures 7.34m. Moreover, the results of the scientific analyses conducted on the soil elements determined that most chemical values were within the defined levels, with the exception of phosphoric acid levels falling within the hyper-nutritious range. Finally, although most of the protective facilities did support the habitation of the Old big tree, there were some supporting pillars and iron pulling-facilities that actually damaged the trees. This research means to provide foundational reference data for the analysis of the habitation and management conditions of natural monument old big tree within the Gangwondo. A follow-up topic led by the results of this research is the identification and analysis of correlating environmental elements that affect the habitation of the old big tree.

Multi-Institute, Single Group, Prospective Observational Study to Evaluate the Efficacy of Mucomyst® in Relieving Symptoms of Globus Pharyngeus Refractory to Proton Pump Inhibitor (양성자펌프억제제에 반응하지 않는 인두이물감 환자에서 "뮤코미스트Mucomyst®" 객담제거 및 상기도 염증치료를 통한 증상개선효과 평가 다기관, 단일군, 전향적 관찰 연구)

  • Park, Hannah;Park, Il-Seok;Lee, Sang Hyuk;Lee, Seung-Won;Lee, Sang Joon;Lee, Byung-Joo;Cheon, Yong-Il;Park, Jun-Ook;Oh, Kyoung Ho;Shin, Yoo Seob
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.33 no.1
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    • pp.20-25
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    • 2022
  • Background and Objectives Globus pharyngeus is one of the most common symptoms of patients visiting otorhinolaryngology out-patient clinic, and usually long-lasting, difficult to treat, and frequently recurrent. Mucomyst®, N-acetyl cysteine is an inhalation agent mainly used for mucolysis and reducing inflammation in airway via antioxidative effect. The purpose of this study was to evaluate the efficacy of inhaled Mucomyst® treatment in patients with globus pharyngeus refractory to proton pump inhibitor (PPI). Materials and Method We prospectively evaluated the efficacy of Mucomyst® in relieving symptoms of globus pharyngeus refractory to PPI in nine medical centers. Three hundred and three patients enrolled and finally 229 patients finished the inhaled Mucomyst® therapy for 8 weeks. We analyzed the change of Reflux Symptom Index (RSI), Reflux Finding Score (RFS), Visual Analogue Scale (VAS) for globus, and Globus Pharyngeus Symptom Scale (GPS) after use of Mucomyst® for 4 and 8 weeks. Results The GPS, RSI, RFS, and VAS score significantly decreased serially in patients who finished 8 week-inhalation treatment. The GPS improvement gap was significantly correlated with initial GPS (p<0.001) in multiple regression analysis. Conclusion Inhaled Mucomyst® therapy was effective for the reduction of both subjective and objective findings in refractory globus patients. This study might suggest new treatment option for patients with globus. However, further thorough studies would be needed to assess the real effect of inhaled Mucomyst® treatment as a standard treatment for globus.