Jo, Yu-Young;Jo, Kyu-Jong;Jin, Yu-Lan;Jung, Woo-Jin;Kuk, Ju-Hee;Kim, Kil-Yong;Kim, Tae-Hwan;Park, Ro-Dong
Journal of Microbiology and Biotechnology
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v.13
no.6
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pp.960-968
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2003
A bacterial isolate showing a strong endochitosanase activity was isolated from soil and then characterized. The isolate was identified and designated as Bacillus cereus P16, based on morphological and biochemical properties, assimilation tests, cellular fatty acids pattern, along with 16S rRNA gene sequence. The optimized medium for producing extracellular chitosanase in a batch culture contained 1% tryptone, 0.5% chitosan, and 1% NaCl (pH 7.0). Powder chitosan and tryptone served the best as carbon and nitrogen sources, respectively, for the chitosanase production. Chitosanase activity was the highest when culture was completed at $37^{\circ}C$ among various temperatures ($20-42^{\circ}C$) tested in a shaking incubator (200 rpm). The levels of chitosanase activity in the culture fluid were 2.0 U/ml and 3.8 U/ml, respectively, when incubated in a flask for 60 h and in a jar fermenter for 24 h. The culture supernatant showed a strong liquefying activity on the soluble chitosan. The viscosity of 1% chitosan solution, that was incubated with the culture supernatant, was rapidly decreased, suggesting the secretion of endochitosanolytic enzymes by P16. The culture fluid revealed six endo-type chitosanase isozymes, two major (38 and 45 kD), and four minor (54, 65, 82, and 96 kD) forms by staining profile. The crude enzymes were very stable, and full activity was maintained for 4 weeks at $4^{\circ}C\;or\;-20^{\circ}C$ in the culture supernatant, suggesting a highly desirable stability rate for making an industrial application of the crude enzymes. The supernatant also cleaved the insoluble chitosan powder, but the hydrolysis rate was much lower. The enzymic degradation products of chitosan contained $(GlcN)_n$ (n=2-8). The concentration of chitosan in the reaction mixture of the crude enzyme affected the chitooligosaccharides composition of the hydrolysis products. When the higher concentration of chitosan was used, the higher degree of polymerized chitooligosaccharides were produced. By comparison with other commercial chitosanase preparations, P16 was indeed found to be a valuable enzyme source for industrial production of chitooligosaccharides from chitosan.
Objectives : This study tried to explore the prevalence of chronic physical diseases in patients with schizophrenia and its effects on total medical costs. Methods : The Health Insurance Review and Assessment Service data in 2014-2015 was employed. Only the injuries and diseases, identically diagnosed 3 times or more as a major or minor injury and disease, were classified into chronic physical diseases to improve data accuracy. Total medical costs included out-of-pocket and insurer's costs from health care system perspective. Results : It was shown that 24.5%, 17.3% and 23.4% of schizophrenia patients had one, two and three or more chronic physical diseases, respectively. There was a high prevalence of not only metabolic, but also musculoskeletal, diseases in those patients. The amount of 2015 total medical costs of patients with schizophrenia in 2014 was about 1.08 trillion won. The factors affecting the costs included sex, age, number of chronic physical diseases, and health insurance status. Conclusions : It is considered that clinical practice guidelines based on personal diseases may not sufficiently solve the problems for comorbidities in schizophrenia patients. Accordingly, it should be required to develop models for new types of medical systems capable of treating and caring varied illnesses at the same time.
A laboratory microcosm experiment was conducted to evaluate a major metal uptake route as well as chronic toxic effects of the clam, Macoma balthica exposed to Ag and Cu contaminated sediments. Twenty five clams were exposed to the sediments contaminated with four levels of Ag $Ag(0.01-0.87\mu{mol}\;g^{-1})$ and $Cu(0.75-5.55\mu{mol\;g^{-1})$ for 90 days. AVS (acid volatile sulfide) concentration in the sediments, considered as major factor controlling metal geochemistry and bioavailability, was manipulated to evaluate its effects on Ag and Cu bioaccumulation in M. balthica. Following 90-d exposure, the tissue Ag and Cu in M. balthica increased linearly with the Ag and Cu concentrations in sediments extracted with 1 N HCI (SEM, simultaneously extracted metals with AVS). The bioaccumulation of Ag and Cu in M. balthica was little influenced by difference in [SEM] - [AVS] values, suggesting a minor contribution of pore water metals to bioaccumulation. Tissue Ag and Cu concentrations directly influenced on the clearance rate and glycogen content of the clams. The clams with highest tissue Ag $(1.0\pm{0.2}\mu{mol}\;g^{-1})$ and Cu concentrations $(2.7\pm{0.3}\;\mu{mol}\;g^{-1})$ had only 18-43% of clearance of the clams exposed to uncontaminated sediments. Similarly, glycogen content of the exposed clams had a inverse relationship with tissue Ag and Cu concentrations. These results suggest that M. balthica exposed to Ag and Cu contaminated sediments accumulates metals largely by ingestion of contaminated sediments and can display chronic effects as reduced clearance rate and glycogen content.
The purpose of this study is to examine current foodservice management practices at free congregate meal service for elderly people. Forty seven meal service centers as well as randomly selected Seoul and Kyunggido area were surveyed and interviewed and results were summarized as follows: The cost of each meal(lunch) was ranged from 1,300 won to 1,500 won and 68% of target centers were severed over 100 meals per day. Meal time for lunch begins from 10:30 am to 12:00 because great portion of elderly didn't take breakfast frequently. 52.3% of centers severed meal 5 times per week, just weekdays. 21.3% of centers employeed dietitian, 63.8% of center employeed cook. 95.7% of center were supported labor force by volunteers. Volunteer was important contribution to free meal service. Utilizing the labor force more effectively is thus a major challenge facing manager in each center. Ideal supporting system of free foodstuff, foodbank was still minor source of securing foodstuff. Most of centers(46 centers)served lunch, only one of them served breakfast and lunch. Government was the major financial sponsor, the second of them was religious organization. The large portions of financial support provided only food cost of total meal service budget. Most of center adapted self-service system. Standardized recipes were not developed and meal preparation was controlled under the experience of volunteers. Recording system of nutrition management, production control, storage and inventory control was not adapted by most of sites. It is suggested that in order to meet the change of the patterns of social and family structure, the service of the center should be offended in urban area and it is necessary to develop systematic management models for the center. It was suggested that not only financial support but also systematical support on management by the local government may be necessary to meet the goal of supply nutritionally balanced food at center.
The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormone-like activity. The material was found to harbor a bacterial community of $2.38{\times}10^8$ CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of ${\beta}$-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.
Journal of Korean Society of Environmental Engineers
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v.37
no.9
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pp.542-549
/
2015
The bioelectrochemical anaerobic digestion for sewage sludge was attempted at different applied voltages ranged from 0.2 V to 0.4 V. At 0.3 V of the applied voltage, pH and VFAs were at 7.32 and 760 mg COD/L, respectively, which were quite stable. The methane production rate was $1.32L\;CH_4/L.d$, and the methane content in biogas was 73.8%, indicating that the performance of the bioelectrochemical anaerobic digestion could be considerably improved by applying a low voltage. At 0.4 V of the applied voltage, however, the contents of the minor VFA components including formic acid and propionic acid were increased. The methane production rate was reduced to $1.24L\;CH_4/L.d$ and the biogas methane content was also reduced to 72.4%. At 0.2 V of the applied voltage, the pH was decreased to 6.3, and VFAs was accumulated to 5,684 mg COD/L. The contents of propionic acid and butyric acid in the VFAs were considerably increased, The performances in terms of the methane production rate and the biogas methane content were deteriorated. The poor performance of the bioelectrochemical reactor at 0.2 V of the applied voltage was ascribed to the thermodynamic potential lack for the driving of the carbon dioxide reduction into methane at cathode.
Oh, Hyung Suk;Kim, Byung Wook;Lee, Si Hun;Nho, Won Ho;Kang, Seung Eun;Ko, Sang Ho
Journal of the Korean Society for Aeronautical & Space Sciences
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v.45
no.12
/
pp.1039-1047
/
2017
This paper presents a test development of a Fly-By-Wireless flight control system for a fixed-wing unmanned aerial vehicle (UAV). Fly-By-Wireless system (FBWLS) refers to a system that uses a wireless network instead of a wired network to connect sensors and actuators with a flight control computer (FCC), reducing considerable amount of wires. FBWLS enables to design a much lighter aircraft along with decreased maintenance time and cost. In this research we developed a Zigbee-based FWBLS UAV in which sensors (GPS and AHRS) are wirelessly connected via a FCC to aileron and elevator servo motors. In order to see the effect of time delay due to wireless signal on the flight stability of the UAV, several flight tests were conducted. From the tests, it was confirmed that the effect is minor by comparing the flight response of the FBWLS with the corresponding Fly-By-Wire system.
In order to artificially reduce the quinclorac residue in aqueous solution and soil, six potential photosensitizers were screened for their effectiveness in enhancing the photodegradation. The degraded amount of quinclorac in distilled water by sunlight was minor compared to that in the dark, indicating that there was little direct photolysis. The photodegradation ratio of quinclorac in methanol was 40.3%. Whereas, the ratios in the presence of photosensitizers PS-1 (aromatic ketone), PS-3 (polycyclic quinone), and PS-6 (inorganic semiconductor) were 96.6, 72.7, and 95.7%, respectively, showing the most photosensitizing effects. In sand, PS-3 was more effective than any other photosensitizer PS-1 (19.6%), PS-3 (64.1%) and PS-6 ($17.9{\sim}19.4%$). five photoproducts of quinclorac in methanol were identified by GC-MS and quinclorac added with the photosensitizer PS-1 gave three photoproducts. Photoproducts with an aldehyde group formed in methanol were confirmed by the reduction of sodium 3,5-dinitrosalicylate in the Lindsay's method. E. crus-galli war. oryzicola was not controlled by the quinclorac residues photodegraded at tile concentrations higher than 30 ppm of the photosensitizer PS-3 in a flooded rice paddy soil. These results indicate that the quinclorac residues in aqueous solution and soil can be degraded efficiently by tile photosensitizers PS-1, PS-3, and PS-6.
Recently, when evaluating the technology values in the fields of biotechnology, pharmaceuticals and medicine, we have needed more to estimate those values in consideration of the period and cost for the commercialization to be put into in future. The existing discounted cash flow (DCF) method has limitations in that it can not consider consecutive investment or does not reflect the probabilistic property of commercialized input cost of technology-applied products. However, since the value of technology and investment should be considered as opportunity value and the information of decision-making for resource allocation should be taken into account, it is regarded desirable to apply the concept of real options, and in order to reflect the characteristics of business model for the target technology into the concept of volatility in terms of stock price which we usually apply to in evaluation of a firm's value, we need to consider 'the continuity of stock price (relatively minor change)' and 'positive condition'. Thus, as discussed in a lot of literature, it is necessary to investigate the relationship among volatility, underlying asset values, and cost of commercialization in the Black-Scholes model for estimating the technology value based on real options. This study is expected to provide more elaborated real options model, by mathematically deriving whether the ratio of the present value of the underlying asset to the present value of the commercialization cost, which reflects the uncertainty in the option pricing model (OPM), is divided into the "no action taken" (NAT) area under certain threshold conditions or not, and also presenting the estimation logic for option values according to the observation variables (or input values).
This study was undertaken to examine the intensity of involvement of inducible nitric oxide synthase (iNOS) and cyclic GMP signal transduction pathway as one of the mechanisms of vaso-relaxative action of bacterial lipopolysaccharide (LPS) on the canine femoral artery strips. Canine femoral arteries were isolated and spiral strips of 10 mm long and 2 mm wide were made in the Tyrode solution of $0-4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with 95% $O_2$/5% $CO_2$ at $37^{\circ}C$ and nitric oxide (NO) production was measured simulltaneously with isolated nitric oxide meter. LPS induced NO production, suppressed the phenylephrine (PE) induced contraction and enhanced the acetylcholine (ACh) induced relaxation. $N^G$-nitro-L-arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue, a guanylyl cyclase inhibitor, potentiated PE induced contraction and suppressed ACh induced relaxation on the LPS treated strips. The inhibitory potency of methylene blue for LPS induced vascular hyporesponsiveness was stronger than that of L-NAME. These results suggest that in canine femoral artery, both iNOS and cyclic GMP signal trnasduction pathway are related with LPS induced vascular hyporeponsiveness, but in minor with iNOS and in major with cyclic GMP signal trnasduction pathway.
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