• Title/Summary/Keyword: S-type pyocin

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Isolation of Pseudomonas aeruginosa 90-2-2205 Producing the S-type Pyocin from Korean Patients and the Pyocin Production (한국환자유래의 S형 Pyocin 생성균주 Pseudomonas aeruginosa 90-2-2205의 분리 및 Pyocin 생산)

  • 김란숙;이정미;김병오;박영덕;진익렬
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.125-131
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    • 1993

  • The s-type pyocin, one kind of bacteriocins, is a bactericidal substabce of protein nature produced by certain Pseudomonas aeruginosa and is active against some other strains of the same or closely related species. Among many Pseudomonas aeruginosa strains collected from the patients at the Hospitals in Seoul and Taegu cities, some Pseudomonas aeruginosa pyocinogeny were determined by pyocin typing. As a result, Pseudomonas aeruginosa 90-2-2205 was selected as the S-type pyocin producing microorganism due to its highest antimicrobial and protease sensitivity.

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Purification and Characterization of the S-type Pyocin of Pseudomonas aeruginwa 90-2-2205 Isolated from Korean Patients (한국환자유래의 녹농균 90-2-2205로부터 S형 Pyocin의 정제 및 특성)

  • 김란숙;이정미;박영덕;진익렬;김병오
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.132-138
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    • 1993

  • The s-type pyocin was purified from the lysate of the mitomycin C-induced Pseudomonas aeruginosa 90-2-2205 cells by the other of ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration. The purity was confirmed by the polyacry-lamide gel electrophoresis. The molecular weight of the purified pyocin was estimated 180, 000 by gel filtration. The pyocin was analyzed to be a complex of some polypeptides by the SDS-PAGE. The pyocin was stable by heat treatment and at pH 6-7.5 by adding 10-3% gelatin and 0.2M NaCl to the 10mM Tris-HCl buffer (pH7.5). Its killing action against the sensitive cells was assumably a single hit process.

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R-Type Pyocin is Required for Competitive Growth Advantage Between Pseudomonas aeruginosa Strains

  • Heo Yun-Jeong;Chung In-Young;Choi, Kelly B.;Cho, You-Hee
    • Journal of Microbiology and Biotechnology
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    • v.17 no.1
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    • pp.180-185
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    • 2007

  • R-type pyocin is a bacteriophage tail-shaped bacteriocin produced by Pseudomonas aeruginosa, but its physiological roles are relatively unknown. Here we describe a role of R-type pyocin in the competitive growth advantages between P aeruginosa strains. Partial purification and gene disruption revealed that the major killing activity from the culture supernatant of PA14 is attributed to R-type pyocin, neither F-type nor S-type pyocins. These findings may provide insight into the forces governing P aeruginosa population dynamics to promote and maintain its biodiversity.

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