• BMB Reports
• /
• 제38권2호
• /
• pp.232-237
• /
• 2005

A glutathione S-transferase (GST) from Lactuca sativa was purified to electrophoretic homogeneity approximately 403-fold with a 9.6% activity yield by DEAE-Sephacel and glutathione (GSH)-Sepharose column chromatography. The molecular weight of the enzyme was determined to be approximately 23,000 by SDS-polyacrylamide gel electrophoresis and 48,000 by gel chromatography, indicating a homodimeric structure. The activity of the enzyme was significantly inhibited by S-hexylGSH and S-(2,4-dinitrophenyl) glutathione. The enzyme displayed activity towards 1-chloro-2,4-dinitrobenzene, a general GST substrate and high activities towards ethacrynic acid. It also exhibited glutathione peroxidase activity toward cumene hydroperoxide.

• 한국식품영양과학회지
• /
• 제20권3호
• /
• pp.203-208
• /
• 1991

The present study was undertaken in order to elucidate the effects of pretreatment with nicotinamide on changes in the hepatic metabolizing enzyme system inducted by streptozotocin (STZ). In rats, STZ(50mg/kg) administered by tail vein caused a significant rise in hepatic aniline hydroxylase and a decrease in aminopyrine N-demethylase when compared to control (p<0.05). Pretreatment with nicotinamice inhibited these effects (p<0.05). Similarly, STZ induced changes in hepatic microsomal cytochrome P-450 activity were inhibited by pretreatment with nicotinamide (p<0.05). However, changes in UDP-glucuronyl transferase and sulfortransferase activity were not significantly different(p>0.05). Pretreatment with nicotinamide also prevented STZ induced increases in glutathion S-tranferase activity when compared to the control(p<0.05). There results suggest that nicotinamide pretreatment suppresses STZ-induced changes in the hepatic metabolizing enzyme system.

• 농약과학회지
• /
• 제10권1호
• /
• pp.50-55
• /
• 2006

본 연구는 강원도 고랭지 배추경작지에서 채집한 chlorpyrifos 포장저항성 계통의 배추좀나방에서 추출한 무독화 효소 esterases와 glutathione-S-tranferase(GST)의 활성과 단백질의 sequestration 비율 및 acetylcholinesterase(AChE) insensitivity를 측정하여 저항성 발달기작을 구명하고자 수행되었다. Esterases의 경우 저항성과 감수성 배추좀나방간의 활성 차이는 없었으나, 전기영동상에서는 특정 isozyme의 차이가 확인되었으며, GST 활성은 포장저항성 계통이 감수성 계통보다 약 1.5배 높았다. 두 계통 간 단백질의 sequestration 차이는 없었으며, chlorpyrifos에 대한 AChE의 sensitivity는 포장저항성 계통의 AChE가 감수성계통보다 약 460배 낮은 것으로 확인되었다. 이러한 결과는 실내에서 저항성을 유도하여 파악한 배추좀나방의 저항성 특성결과와 동일한 것으로 강원도 고랭지 chlorpyrifos 포장저항성 배추좀나방의 저항성 발달은 AChE의 insensitivity가 주요 요인이며, 부가적으로 GST의 활성 변화도 작용하는 것으로 판단되었다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제28권2호
• /
• pp.85-91
• /
• 2014

This study was designed to find out the effect of Nosema spore on oxidative damages and antioxidant defence in the midgut of tasar silkworm Antheraea mylitta. Higher level of lipid peroxidation (LPX) and total hydroperoxides indicate the resultant oxidative stress in the Nosema exposed specimen. Increased superoxide dismutase (SOD) suggests activation of physiological mechanism to scavenge the superoxide radical produced during Nosema infection. Higher activities of catalase and glutathione-S-tranferase on $18^{th}$ d indicate adaptive behaviour of the tissue against oxyradicals. The results suggest that Nosema infection is involved in altering the active oxygen metabolism by modulating LPX and reactive oxygen species (ROS), which is indicative of pebrine disease disorder.

• 분석과학
• /
• 제10권5호
• /
• pp.378-385
• /
• 1997

본 실험은 human glutathione S-transferase P1-1의 tyrosine 7 잔기에 대한 변이체를 작성하고, 기질특이성과 저해제의 효과를 조사하여, 이 잔기의 기능을 분석한 것이다. 1,2-dichloro-4-nitrobenzene과 1,2-epoxy-3-(p-nitrophenoxy)propane에 대한 GSH 포합반응에 대한 활성은 야생형에 비해 변이체 Y7F에서는 3~5%로 크게 저하하였으며, 효소에 결합한 GSH의 thiol기의 pKa는 2.4 pK 높았다. 저해제 hematin에 대한 $I^{50}$값은 야생형과 변이체 Y7F에서 비슷하게 나타났으며, 저해제 benastatin A와 S-(2,4-dinitrophenyl) glutathione에 대한 $I^{50}$값들은 다소 감소하였다. 이러한 결과들로부터 tyrosine 7 잔기는 기질의 결합에 관여하기보다 GSH-chloronitrobenzene 유도체와 GSH-epoxide 포함반응에 대한 촉매활성에 중요하다고 생각된다.

• 분석과학
• /
• 제22권3호
• /
• pp.228-234
• /
• 2009

본 연구에서는 식물의 제초제 해독 기구를 알아보기 위해, 양배추 (Brassica oleracea)로 부터 글루타티온 전달효소를 정제하고 외래성 이물질에 대한 기질 특이성과 저해 효과를 분석하였다. 양배추로부터 DEAE-Sephacel 컬럼과 GSH-Sepharose 컬럼 크로마토그래피를 이용하여 약 10%의 수율로 글루타티온 전달효소를 정제하였다. 정제된 효소에 대해 분자량을 측정한 결과, SDS-polyacrylamide 겔 전기영동으로 측정한 분자량은 23,000Da을 나타내었으며, 겔 크로마토그래피로 측정한 분자량은 48,000Da을 나타내었다. 따라서 정제된 양배추 글루타티온 전달효소는 소단위체의 분자량이 약 23,000Da의 동종이량체라는 사실을 알 수 있었다. 이 효소의 저해제에 대한 효과를 조사한 결과, S-hexyl-GSH와 S-(2,4-dinitrophenyl)GSH에 의해 활성이 저해되었다. 양배추 글루타티온 전달효소의 기질특이성은 CDNB와 ETA에서 높은 활성을 보였으며, cumene hydroperoxide에 대한 GSH peroxidase 활성도 나타내었다.

• 한국임상약학회지
• /
• 제16권2호
• /
• pp.155-164
• /
• 2006

Great inter-variability in drug response and adverse drug reactions is related to inter-variability of drug bioavailability, drug interaction and patient's disease and physyological state that cause change in absorption, distribution, metabolism and excretion of drugs. However, these alone do not sufficiently predict and explain inter-variability in drug response. In recent studies, it is reported that inter-variability in drug response and adverse drug reactions may largely resulted from genetically determined differences in drug absoption, distribution, metabolism and drug target proteins. Especially, the major human drug-metabolizing enzymes such as CYP450, N-acetyl tranferase, thiopurine S-methyl transferase, glutathione S-transferase are identified as the major gene variants that cause inter-individual variability in drug's response and adverse drug reactions. These variations may have most significant implications for those drugs that have narrow therapeutic index and serious adverse drug reactions. Therefore, the genetic variation such as polymorphisms in drug metabolizing enzymes can affect the response of individuals to drugs that are used in the treatment of depression, psychosis, cancer, cardiovascular disorders, ulcer and gastrointestinal disorders, pain and epilepsy, among others. This review describes the pharmacogenomics of the drug metabolizing enzymes associated with the drug response and its clinical applications.

• 대한수의학회지
• /
• 제34권3호
• /
• pp.609-617
• /
• 1994

This study was performed for assessing carcinogenicity of Folpet using medium-term carcinogenicity bioassay. Sprague-Dawley rats aged six weeks divided into four grout's and were initially given an intraperitoneal injection of diethylnirosamine at 200mg/kg body weight. Two weeks later, group 1(negative control) was treated with basal diet. A Folpet was given per oral administration to group 2(100 ppm) and goup 3(1,000 ppm). Group 4 was fed on water containing 0.05% phenobarbital sodium as a promtor for six weeks. At three weeks after beginning of the experiment, partial hepatectomy was performed in all rats. The tumor-promoting effects were examined by the numbers and areas per $cm^2$ of induced glutathion S-tranferase placetal form(GST-P) positive foci in liver, and silver stained nucleolar organizer regions(AgNORs) which have recently introduced as one of the indicators for the cell proliferative activity. As the results, Folpet didn't have tumor-promoting effects on GST-P positive foci developement and AgNORs during promoting stage after initiation, whereas phenobarbital sodium treatment group showed promoting effect. It was concluded that Folpet didn't have promoting effect at 500, 1,000 ppm using this midium-term carcinogenicity bioassay model.

• 대한약리학회지
• /
• 제21권1호
• /
• pp.1-11
• /
• 1985

이물질(xenobiotics) 대사에 관여하는 간장 microsome과 cytosol 효소 활성에 indole, indole-3-carbinol 및 benzofuran이 미치는 영향을 검색하기위하여 마우스에 이들 약물을 각각 5 mmole/kg씩 10일간 투여하여 다음 몇 가지의 성적을 얻었다. Benzofuran은 microsome 효소인 aniline hydroxylase, 7-ethoxycoumarin O-deethylase, p-nitrophenol UDPGA-transferase, epoxide hydrolase와 cytosol 효소인 glutathione S-tranferase, NADH : quinone reductase, UDP-glucose dehydrogenase의 활성도를 증가시켰다. 그러나 benzofuran과는 구조적으로 furan ring내의 N원소가 O원소로 치환되었을 뿐 주된 구조가 유사한 indole과 indole-3-carbinol 투여로는 UDPGA-transferase와 NADH: quinone reductase의 활성도 증가를 볼 수 없었으며, 특히 indole은 NADPH : cytochrome C reductase만을 증가시킨데 비하여 구조상 indole에 carbinol (methanol)기가 붙은 indole-3-carbinol은 수종의 mixed function oxidase와 아울러 특히 epoxide hydrolase의 활성도 역시 증가시켰다. 이러한 결과는 benzofuran과 indole-3-carbinol에 의한 epoxide hydrolase 활성도 증가의 기전의 일부를 설명할 수 있을 것으로 생각된다.

• Applied Biological Chemistry
• /
• 제51권4호
• /
• pp.309-315
• /
• 2008

돌단풍 지상부를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. 이 중 EtOAc 및 n-BuOH 분획으로부터 silica gel chromatography를 반복하여 5개의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, astragalin (1), kaempferol 3-O-${\alpha}$-L-rhamnopyranosyl (1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (2), rutin(3), kaempferol 3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$4)-${\alpha}$-Lrhamnopyranosyl(1${\rightarrow}$6)-${\beta}$-D-glucopyranoside(4), quercetin 3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$4)-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$6)-${\beta}$-Dglucopyranoside(5)로 동정하였다. 이들 화합물은 FPTase 활성을 억제하였으며, 특히 화합물 3(rutin)은 rat H-ras 세포주의 생장과 bFGF로 유도시킨 HUVECs의 cell migration을 억제하는 것으로 나타났다.