• Journal of Ginseng Research
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• 제46권1호
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• pp.167-174
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• 2022

Background: 20(S)-protopanaxadiol (20(S)-PPD), one of the main active metabolites of ginseng, performs a broad spectrum of anti-tumor effects. Our aims are to search out new strategies to enhance anti-tumor effects of natural products, including 20(S)-PPD. In recent years, fasting has been shown to be multi-functional on tumor progression. Here, the effects of fasting combined with 20(S)-PPD on hepatocellular carcinoma growth, apoptosis, migration, invasion and cell cycle were explored. Methods: CCK-8 assay, trypan blue dye exclusion test, imagings photographed by HoloMonitorTM M4, transwell assay and flow cytometry assay were performed for functional analyses on cell proliferation, morphology, migration, invasion, apoptosis, necrosis and cell cycle. The expressions of genes on protein levels were tested by western blot. Tumor-bearing mice were used to evaluate the effects of intermittent fasting combined with 20(S)-PPD. Results: We firstly confirmed that fasting-mimicking increased the anti-proliferation effect of 20(S)-PPD in human HepG2 cells in vitro. In fasting-mimicking culturing medium, the apoptosis and necrosis induced by 20(S)-PPD increased and more cells were arrested at G0-G1 phase. Meanwhile, invasion and migration of cells were decreased by down-regulating the expressions of matrix metalloproteinase (MMP)-2 and MMP-9 in fasting-mimicking medium. Furthermore, the in vivo study confirmed that intermittent fasting enhanced the tumor growth inhibition of 20(S)-PPD in H22 tumor-bearing mice without obvious side effects. Conclusion: Fasting significantly sensitized HCC cells to 20(S)-PPD in vivo and in vitro. These data indicated that dietary restriction can be one of the potential strategies of chinese medicine or its active metabolites against hepatocellular carcinoma.

• 한국의상디자인학회지
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• 제16권3호
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• pp.201-217
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• 2014

There is likely to be a fit problem when women aged in their 30s-40s purchase down-age clothing from brands that target women in their 20s. To identify the part of the lower body that causes the fit problem, the sizes and types of lower body of women in their 30s-40s were compared with those of women in their 20s. The data for this study was from the 2010 Size Korea survey of body measurements of 1675 female adults in their 20s-40s. To examine differences in the average sizes of the lower body between women in their 20s and 30s-40s, descriptive statistics and t-tests were conducted. Factor and cluster analysis were used to classify body types by age groups. On the whole, compared to women in their 30s-40s, women in their 20s were found to be higher for all height items and smaller for size, thickness and width items. The result of the Glycemic index analysis showed that the body type of women in their 20s was generally larger than that of women in their 30s-40s. In addition, women in their 20s had a large drop value compared to those in their 30s-40s, whereas flatness of body cross section was bigger in the group of women in their 30s-40s. Four factors related to the lower body type of female adults in their 20s-40s were identified in the factor analysis, and three categories of body type were identified by cluster analysis. A Type 1 individual had abdominal obesity and chubby lower limbs, Type 2 had short legs and slightly chubby lower limbs, and Type 3 had long legs and a skinny lower body. A significant number of women in their 30s-40s were included in the Type 1 group, which was the least common category for women in their 20s.

• 한국식품영양학회지
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• 제33권3호
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• pp.237-250
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• 2020

In this study, the Korea National Health and Nutrition Examination Survey in 2016 examined the nutrient and food intake of women in their 20s and 30s according to the number of meals a day, and the results were as follows: The study included 256 women in their 20s and 474 women in their 30s. Women in their 20s and 30s ate two meals a day, indicating that the notion of three meals a day was gradually changing to two to three meals a day. Those in their 20s and 30s who eat one, two or three meals a day were consuming less than the estimated energy requirement of the DRI for Koreans 2015. The vitamin A and C were eating less than the recommended intake in all meals. In the case of minerals, calcium was taken less than the recommended intake in all meals, but sodium was taken above the goal intake in all groups. Women in their 20s and 30s must reduce their intake of saturated fatty acids and sodium, and increase their intake of vitamins A, C, calcium, and potassium. Women in their 20s and 30s drank a lot of coffee, ate baechu-kimchi and rice frequently per week.

• 한국안광학회지
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• 제17권3호
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• pp.273-278
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• 2012

목적: 20대와 40대 초반 근시안의 성별 및 근시도에 따른 최대조절력의 평균 크기를 비교 분석하여 세대간 조절력의 변화를 알아보고자 하였다. 방법: 20대 초반과 40대 초반의 근시안 각 100명을 대상으로 하여 푸쉬업법(pushup method)을 이용하여 최대조절력을 측정하였다. Hofstetter 공식을 이용하여 얻어진 최대조절력 기대값을 기준으로 기대최소치 미만군(under), 정상군(normal), 기대최대치 초과군(excess)으로 분류하여 비교하였다. 결과: 20대 초반의 평균 조절력은 9.77~11.64 D$(10.77{\pm}2.49D)$, 40대 초반의 평균 조절력은 4.67~6.21 D$(5.34{\pm}1.28D)$로 나타났고, 20대 초반에서 조절력의 기대최소치 미만군은 20%, 정상군 75%, 기대최대치 초과군 5%의 분포를 나타내었고, 40대 초반에서는 조절력의 기대최소치 미만군 18%, 정상군 82%를 나타내었고, 기대최대치 초과군은 나타나지 않았다. 결론: 두 연령층 모두에서 연령이 증가함에 따라 조절력의 감소를 나타내었는데, 근시도와 성별에 따른 유의성은 나타나지 않았다. 또한 각 연령대별로 최대조절력의 기대평균치와 최대조절력 평균의 차를 비교해 보았을 때 20대 초반에서 기대평균치보다 더 작은 것으로 나타나 상대적으로 근업 기회가 많은 20대의 조절력 저하가 훨씬 빨리 나타날 것으로 예상된다.

• 약학회지
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• 제49권2호
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• pp.122-127
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• 2005

Dental caries is one of the most common oral diseases in the world. Glucosyltransferase (Gtase) of Streptococcus mutans (S. mutans) plays an important role in the develo pment of dental caries. For the purpose to develop anti- caries, we examined the effect of metabolites isolated from Streptomyces sp. M-20 on Gtase and the growth of S. mutans. Streptomyces sp. M-20 isolated from Mongolian soil showed 95~96% sequence homology with that of Streptomyces lin- colnensis. The metabolites of Streptomyces sp. M-20 were partially purified by extraction with ethyl acetate, silica gel column chromatography and preparative TLC. Partially purified metabolite, red colored component (MR-20) in ethyl acetate fraction showed potent antibacterial activitiy against S. mutans and inhibitory activity against Gtase purified from S. mutans, while another isolated yellow component (MY-20) showed no activity against S. mutans. The inhibitory activity of MR-20 against Gtase was confirmed by activity staining on SDS-polyacrylamide gel electrophoresis. The concentration of MR-20 for 50% inhibition $(IC_{50})$ against Gtase activity was $60{\mu}g/ml$. These results suggest that MR-20 can be developed for antibacterial agent and anticaries.

• 복식
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• 제55권4호
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• pp.16-28
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• 2005

The purposes of this study were to understand the buying patterns of adult women according to impulse shopping, benefits sought, and selecting information sources. This subjects of this study were 509 adult women from the age 20's to the age of 40's. This data were analyzed by using factor analysis multi regression analysis, analysis of variance, Scheffe' test. The results of this study were outlined as follow: 1. Differences among ages (the 20's, the 30's, and the 40's) in impulse shopping were as follow: The 30's bought more cosmetics to change their mood compared to the 20's or the 40's, and the 20's and the 30's bought more cosmetics matching to their images compared to the 40's. 2. From the aspects of benefits sought, the 20's and the 30's were more seriously consider the fame of brands compared to the 40's, showing they were different from the 40's. 3. From the aspects of selecting information sources about cosmetics, the 20's were more actively utilizing both human information sources and non-human information sources compared to the 30's and the 40's.

• 한국양식학회지
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• 제9권4호
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• pp.321-327
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• 1996

Production of resting egg from the Korean rotifer, Brachiunus plicatilis (L and S-type) was investigated at different temperatures (L-type : 20, 24, $28^{\circ}C$, S-type : 28 32, $36^{\circ}C$) and salinities (10, 20,30 ppt). The rotifer was cultured in 25 ml test tube and fed on Nannochloris oculata. With regard to mixis rate, L-type rotifer showed higher rate at lower temperature, and the highest rate was observed at 20 ppt of salinity at each temperature of the experiment. However, for S-type rotifer, the optimum temperature and salinity were $28\~32^{\circ}C$ and 20 ppt, respectively. The highest number of resting egg was 173 eggs/ml in 16 days at $24^{\circ}C$, 10 ppt for L-type rotifer and 410 eggs/ml in 14 days at $28^{\circ}C$, 10 ppt for S-type rotifer. The maximum number of resting egg produced per 10,000 rotifers was 8,122 eggs at $20^{\circ}C$, 20 ppt for L-type rotifer and 8,700 eggs at $28^{\circ}C$, 20 ppt for S-type rotifer. The maximum number of resting egg produced $10^8$ cells of N. oculata was 50.7 eggs for L-type rotifer ($24^{\circ}C$, 20 ppt) and 79.6 eggs for S-type rotifer ($32^{\circ}C$, 10 ppt). The number of resting egg produced per day was $1\~11$ eggs/ml for L-type rotifers and $21\~35$ eggs/ml for S-type rotifer in 9 combination experiments. In this study, S-type rotifer is better than L-type rotifer in resting egg production, and the optimum temperature and salinity for resting egg production were $20^{\circ}C$, 20 ppt for L-type rotifer and $28^{\circ}C$, 20 ppt for S-type rotifer. This result shows the difference of Korean rotifer in the optimum condition for resting egg production from other rotifers reported earlier.

• 복식
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• 제51권7호
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• pp.111-121
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• 2001

The purpose of this study was to investigate the historical changes, and analyze functions and characteristics of western men's underclothes in the second half of the 20C. This study will examine how the social backgrounds influenced on the changes of the functions of men's underclothes and how the changes of outer garments were reflected on underclothes in the second half of the 20C. In the early, the second half of the 20C, underwears of functions was continued to cleanliness and comforts. In the later of the 20C, underwears came to be seen as expressing male sexual power when it became body-conscious. By the changes of outer garments. underclothes changed the same size of proportion. In the late 1960's, the turtleneck type of shirts were copied by outer garments, and we understand that underwear can influence on outer garments. The ruffles of the 18th century reappeared in the 1960's. This reactionism of the late 20C tells us about the relation between social environments and underwear. So the social backgrounds influenced on the formativeness of men's underclothes. We understand that underwear can influence on outer garments and outer garments can influence on underwear, too. The important characteristics of the second half of the 20C were the remarkable resemblance of some styles to those of the Past and the occasional blurring of the distinction between underwear and outerwear.

• 한국의류학회지
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• 제32권1호
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• pp.134-146
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• 2008

This study measured the body parts of female adults in their 20s, 40s and 60s living in Japan and analyzed and examined the measures by age group in order to provide the basic data required for the somatics study of Koreans living abroad. The subjects were 304 women in their 20s, 40s and 60s born in Japan and living in Osaka at present, and 81 items were measured. The major results of this study are described below. All women in their 20s, 40s and 60s showed significant differences in weight, waist size and abdominal size. The 19 height items of which group were different from the measurements of the women in their 60s. All 20 length items displayed a significant difference by age group. The significant differences by age group were also observed in eight items of 12 width items, 10 items of 13 thickness items and 13 items of 14 girth items except the ankle girth. The weight measurements had a significant difference by age group(${\le}0.05$). The women in their 20s, 40s and 60s revealed almost similar values in the shoulder angles. As a result of the analysis above, the items that were considered being included in the obesity items in all age groups displayed significant personal differences. The women in their 20s had the largest sizes in the length and height items. The older the age, the higher the measures of width and girth items were. Those results helped to estimate the change of figures by torso by age.

• Environmental Engineering Research
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• 제25권1호
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• pp.96-103
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• 2020

The aim of this study is to investigate the effect of five different combinations including: sand 70%, bentonite 30% (S₇₀B₃₀)- sand 80%, bentonite 20% (S₈₀B₂₀)- sand 80%, organophilic 20% (S₈₀M₂₀)- sand 60%, bentonite 20%, organophilic 20% (S₆₀B₃₀M₂₀) and sand 75% - bentonite 15% - organophilic 10% (S₇₅B₁₅M₁₀) on landfill linear structure in order to decrease phenol leaching. Hydraulic conductivity and adsorption behavior of the samples were investigated. The results demonstrated that the lowest hydraulic conductivity coefficient ($1.16{\times}10^{-11}{\frac{m}{s}}$) was obtained for S₇₀B₃₀. Furthermore, adding more than 20% of bentonite had no significant effect on reducing permeability. Moreover, Freundlich isotherm was introduced as the best model explaining adsorption behaviour due to its highest determination coefficient (0.945). The best samples for adsorption capacity of phenol and for both permeability and adsorption are S₈₀M₂₀ and S₆₀B₃₀M₂₀, respectively. Although the presence of bentonite was effective in reducing hydraulic conductivity, organic clay had no considerable impact on reducing permeability. Though, it's an exceptional role in adsorbing organic contaminants including phenol cannot be ignored. To meet all regulatory constraints, the optimal compound is made up of 10.2% of bentonite and 2.8% of organophilic clays with a minimized cost of 13.64 ($/ton).