• Journal of Microbiology and Biotechnology
• /
• 제29권11호
• /
• pp.1769-1776
• /
• 2019

Ethyl (S)-3-hydroxy-3-(2-thienyl) propanoate ((S)-HEES) acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which can be achieved via the stereoselective bioreduction of ethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that contains a 3-oxoacyl structure. The sequences of the short-chain dehydrogenase/reductases from Chryseobacterium sp. CA49 were analyzed, and the putative 3-oxoacyl-acyl-carrier-protein reductase, ChKRED12, was able to stereoselectively catalyze the NADPH-dependent reduction to produce (S)-HEES. The reductase activity of ChKRED12 towards other substrates with 3-oxoacyl structure were confirmed with excellent stereoselectivity (>99% enantiomeric excess) in most cases. When coupled with a cofactor recycling system using glucose dehydrogenase, the ChKRED12 was able to catalyze the complete conversion of 100 g/l KEES within 12 h, yielding the enantiopure product with >99% ee, showing a remarkable potential to produce (S)-HEES.

• ALGAE
• /
• 제35권1호
• /
• pp.1-15
• /
• 2020

Two strains isolated from a subtropical region in China, were morphologically identified as a Nostoc-like species, but its taxonomic identity was unknown. In this study, these two strains were taxonomically and phylogenetically characterized based on polyphasic approach combining morphological and genetic characteristics. Though both were virtually indistinguishable from Nostoc in field and cultured material, these two strains were phylogenetically distinct from Nostoc based on 16S rRNA phylogeny. The 16S-23S internal transcribed spacer rRNA secondary structure of these strains showed the unique pattern of D1-D1', Box-B, and V3 helix, which distinguished them from other Nostoc-like heterocytous genera. A unique cluster separated from Nostoc sensu stricto supports the establishment of Violetonostoc gen. nov. with the type species as Violetonostoc minutum sp. nov.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제17권8호
• /
• pp.1996-2015
• /
• 2023

High-definition (HD) maps can provide precise road information that enables an autonomous driving system to effectively navigate a vehicle. Recent research has focused on leveraging semantic segmentation to achieve automatic annotation of HD maps. However, the existing methods suffer from low recognition accuracy in automatic driving scenarios, leading to inefficient annotation processes. In this paper, we propose a novel semantic segmentation method for automatic HD map annotation. Our approach introduces a new encoder, known as the convolutional transformer hybrid encoder, to enhance the model's feature extraction capabilities. Additionally, we propose a multi-level fusion module that enables the model to aggregate different levels of detail and semantic information. Furthermore, we present a novel decoupled boundary joint decoder to improve the model's ability to handle the boundary between categories. To evaluate our method, we conducted experiments using the Bird's Eye View point cloud images dataset and Cityscapes dataset. Comparative analysis against stateof-the-art methods demonstrates that our model achieves the highest performance. Specifically, our model achieves an mIoU of 56.26%, surpassing the results of SegFormer with an mIoU of 1.47%. This innovative promises to significantly enhance the efficiency of HD map automatic annotation.

• Journal of Microbiology and Biotechnology
• /
• 제25권5호
• /
• pp.672-680
• /
• 2015

As a global regulatory gene in Streptomyces, afsR can activate the biosynthesis of many secondary metabolites. The effect of afsR on the biosynthesis of a phenazine metabolite, lomofungin, was studied in Streptomyces lomondensis S015. There was a 2.5-fold increase of lomofungin production in the afsR-overexpressing strain of S. lomondensis S015 N1 compared with the wild-type strain. Meanwhile, the transcription levels of afsR and two important genes involved in the biosynthesis of lomofungin (i.e., phzC and phzE) were significantly upregulated in S. lomondensis S015 N1. The optimization of metal chlorides was investigated to further increase the production of lomofungin in the afsR-overexpressing strain. The addition of different metal chlorides to S. lomondensis S015 N1 cultivations showed that CaCl₂, FeCl₂, and MnCl₂ led to an increase in lomofungin biosynthesis. The optimum concentrations of these metal chlorides were obtained using response surface methodology. CaCl₂ (0.04 mM), FeCl₂ (0.33 mM), and MnCl₂ (0.38 mM) gave a maximum lomofungin production titer of 318.0 ± 10.7 mg/l, which was a 4.1-fold increase compared with that of S. lomondensis S015 N1 without the addition of a metal chloride. This work demonstrates that the biosynthesis of phenazine metabolites can be induced by afsR. The results also indicate that metal chlorides addition might be a simple and useful strategy for improving the production of other phenazine metabolites in Streptomyces.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권5호
• /
• pp.3375-3376
• /
• 2013

• Nuclear Engineering and Technology
• /
• 제49권8호
• /
• pp.1748-1751
• /
• 2017

Reduced activation ferritic/martensitic (RAFM) steels are widely applied as structural materials in the nuclear industry. To investigate hydrogen's effect on RAFM steels' elastic properties and the mechanism of that effect, a procedure of first principles simulation combined with experiment was designed. Density functional theory models were established to simulate RAFM steels' elastic status before and after hydrogen's insertion. Also, experiment was designed to measure the Young's modulus of RAFM steel samples with and without hydrogen charging. Both simulation and experiment showed that the solubility of hydrogen in RAFM steels would decrease the Young's modulus. The effect of hydrogen on RAFM steels' Young's modulus was more significant in water-quenched steels than it was in tempering steels. This indicated that defects inside martensite, considered to be hydrogen traps, could decrease the cohesive energy of the matrix and lead to a decrease of the Young's modulus after hydrogen insertion.

• 한국정보통신학회논문지
• /
• 제12권6호
• /
• pp.1026-1031
• /
• 2008

현재 가정과 소규모 사업장에서 재정적인 변화와 개인 커뮤니케이션 그리고 원격의료에 이르기까지 점점 GPON 사용이 일반화 되어가고 있다. 이러한 PON의 다중사용 때문에 개인정보 보호와 커뮤니케이션 보호를 위한 보안의 필요성이 더더욱 커지고 있다. 이를 위해 이 논문에서는 Virtex4 FPGA를 기반으로 AES의 카운터 모드를 구현하였다. 본 논문에서 구현된 구조는 pipeline 구조 구현을 위하여 크게 세 가지 특징을 가지고 있는데 1) composite filed 연산을 이용한 Subbyte, 2) efficient MixColumn transformation, 그리고 3) on-the-fly key scheduling이다. 구현된 S-box는 면적의 17% 감소와 on-the-fly key 스케줄링 기법으로 pipeline 구조에 특화된 key-expander 기능을 구현하였다.

• Biomolecules & Therapeutics
• /
• 제27권5호
• /
• pp.442-449
• /
• 2019

This study sought to evaluate the effects of Asiatic acid in LPS-induced BV2 microglia cells and 1-methyl-4-phenyl-pyridine ($MPP^+$)-induced SH-SY5Y cells, to investigate the potential anti-inflammatory mechanisms of Asiatic acid in Parkinson's disease (PD). SH-SY5Y cells were induced using $MPP^+$ to establish as an in vitro model of PD, so that the effects of Asiatic acid on dopaminergic neurons could be examined. The NLRP3 inflammasome was activated in BV2 microglia cells to explore potential mechanisms for the neuroprotective effects of Asiatic acid. We showed that Asiatic acid reduced intracellular production of mitochondrial reactive oxygen species and altered the mitochondrial membrane potential to regulate mitochondrial dysfunction, and suppressed the NLRP3 inflammasome in microglia cells. We additionally found that treatment with Asiatic acid directly improved SH-SY5Y cell viability and mitochondrial dysfunction induced by $MPP^+$. These data demonstrate that Asiatic acid both inhibits the activation of the NLRP3 inflammasome by downregulating mitochondrial reactive oxygen species directly to protect dopaminergic neurons from, and improves mitochondrial dysfunction in SH-SY5Y cells, which were established as a model of Parkinson's disease. Our finding reveals that Asiatic acid protects dopaminergic neurons from neuroinflammation by suppressing NLRP3 inflammasome activation in microglia cells as well as protecting dopaminergic neurons directly. This suggests a promising clinical use of Asiatic acid for PD therapy.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제10권5호
• /
• pp.2394-2406
• /
• 2016

ABE has become an effective tool for data protection in cloud computing. However, since users possessing the same attributes share the same private keys, there exist some malicious users exposing their private keys deliberately for illegal data sharing without being detected, which will threaten the security of the cloud system. Such issues remain in many current ABE schemes since the private keys are rarely associated with any user specific identifiers. In order to achieve user accountability as well as provide key exposure protection, in this paper, we propose a key-insulated ciphertext policy attribute based encryption with key exposure accountability (KI-CPABE-KEA). In our scheme, data receiver can decrypt the ciphertext if the attributes he owns match with the self-centric policy which is set by the data owner. Besides, a unique identifier is embedded into each user's private key. If a malicious user exposes his private key for illegal data sharing, his identity can be exactly pinpointed by system manager. The key-insulation mechanism guarantees forward and backward security when key exposure happens as well as provides efficient key updating for users in the cloud system. The higher efficiency with proved security make our KI-CPABE-KEA more appropriate for secure data sharing in cloud computing.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제15권9호
• /
• pp.3322-3347
• /
• 2021

Cloud Storage is the primary component of many businesses on cloud. Majority of the enterprises today are adopting a multicloud strategy to keep away from vendor lock-in and to optimize cost. Auditing schemes are used to ascertain the integrity of cloud data. Of these schemes, only the Provable Data Possession schemes (PDP) are resilient to key-exposure. These PDP schemes are devised using Public Key Infrastructure (PKI-) based cryptography, Identity-based cryptography, etc. PKI-based systems suffer from certificate-related communication/computational complexities. The Identity-based schemes deal with the exposure of only the auditing secret key (audit key). But with the exposure of both the audit key and the secret key used to update the audit key, the auditing process itself becomes a complete failure. So, an Identity-based PDP scheme with Parallel Key-Insulation is proposed for multiple cloud storage. It reduces the risk of exposure of both the audit key and the secret key used to update the audit key. It preserves the data privacy from the Third Party Auditor, secure against malicious Cloud Service Providers and facilitates batch auditing. The resilience to key-exposure is proved using the CDH assumption. Compared to the existing Identity-based multicloud schemes, it is efficient in integrity verification.