Oxygen and hydrogen isotopic compositions of stream water in the Han river basin are expressed by the equation of $\delta$D=6.6$\delta$$^{18}$ O-7.4, which is not satisfy the meteoric water line ($\delta$D=8$\delta$$^{18}$ O+10). It might be depended on the local climatic condition and the evaporation effect in the Han river basin. The $\delta$$^{18}$ O and $\delta$D values of stream water in the Han river basin range from -8.2 to -10$\textperthousand$ (avg. -9.1$\textperthousand$) and -60 to -96$\textperthousand$ (avg. -69$\textperthousand$), respectively. The stream water from the South Han river (8$\delta$$^{18}$ O= -8.9~ -10$\textperthousand$, avg.-9.3$\textperthousand$$\delta$D: -66~ -96$\textperthousand$, avg.-69$\textperthousand$) is slightly more depleted in $^{18}$ O and D than those of North Han river ($\textperthousand$$^{18}$ O= -8.4~ -9.7$\textperthousand$, avg. -9.2$\textperthousand$, $\delta$D= -64~ -95$\textperthousand$, avg. -69$\textperthousand$). It reflects more altitude effect than the effect of latitude and Inflow of the $^{18}$ O eniched S $O_4$$^{2-}$ and HC $O_3$- from the carbonate rock and sulfide minerals in the Taebagsan and Hwanggangri mineralized zone. The Main stream water of the Han river having $\delta$D: -60~ -76$\textperthousand$ (avg.-68$\textperthousand$) and $\textperthousand$$^{18}$ O= -8.2~-10$\textperthousand$ (avg.9.0$\textperthousand$) is enriched in $^{18}$ O compared to the South and North Han river waters, which is caused by the evaporation effect. Binary simple mixing ratio of the Main Han river water between South and North Han river waters was obtained to be 6 : 4 by the isotopic data, suggesting a strong influence of South Han river water to the Main Han river water.
The essential factor of acute respiratory distress syndrome (ARDS), an acute lung injury accompanied commonly by sepsis syndrome is accumulation of neutrophils in lung tissue. The study attempted to confirm whether a lung injury would be decreased with the anti-inflammatory effect of germanium by the treated germanium prior to the development of ARDS and whether nitric oxide influence in suppressing a lung injury. Test groups were divided in the following structure for experiment; CON that has been administered with sodium chloride to airway, LPS administered with endotoxin for 5 hours in the same amount and 5 hours of endotoxin administered Ge+LPS following 1 hours of pre-treated germanium. The result of a test using experimental animals, infilteration of neutrophils (p<0.001) in bronchoalveolar lavage fluid (BALF) was significantly decreased, the structure of lung tissue was preserved relatively well, and much neutrophils with distinct positive were observed on tunel staining which showed increase of apoptotic neutrophils in the pre-treated germanium group compare to the endotoxin administrated group. In observation of ultrastructural changes of cell in BALF, phagocytic alveolar macrophage was increased in alveolar space, the nucleus of most engulfed neutrophils were condensed, and some apoptosis neutrophils appears to be DNA fragmentation and effacement of cellular organelles were found in intercellular matrix in the pre-treated germanium group. However, the nitric oxide showed increase in all the groups excluding CON, and the nitric oxide effect such as degranulation diminishing of mast cells and apoptosis increase of neutrophils in the pre-treated group only. The situation appears that there was change in internal environment of the experimental animal by the pre-treated germanium before the nitric oxide is produced and the anti-inflammatory effect activated the pre-processed germanium by nitric oxide which activated following the change. Therefore, the nitric oxide created from macrophage in accordance with the pre-treated germanium appears to influence in alleviating a lung injury. Accordingly, acute lung injury is alleviated by the anti-inflammatory effect of germanium such as inhibition of neutrophils migration, induction of neutrophil apoptosis and increase of phagocytic function of phagocyte, and the nitric oxide produced from activated macrophage by germanium would influence in suppressing a lung injury.
Kim, Sang-Rok;Kim, Gon-Sik;Woo, Jae-Hoon;Lee, Jun-Woo;Sung, Kyung-Il
Journal of The Korean Society of Grassland and Forage Science
/
v.24
no.3
/
pp.253-260
/
2004
The purpose of this study is to discuss the ways to evaluate the effectiveness of storage sections of the round bale silage of fresh rice straw (RS). This study evaluated, the changes of the fermentation characteristics and chemical composition of the different sectors of the RS after a certain period; a month later after the bailing, samples of three different RS (bale one, bale two, and bale three) were taken according to the three different sectors (top, center, and the bottom) of the bale. According to the findings of this study there was no significant difference in the RS's chemical composition content among the different sectors (top, center, or the bottom) of the bale. The possible reason for this is believed that on the basis of different sectors of the bale, the moisture (the water soluble nutrient) movement didn't occur and was locked in and couldn't escape, because the dry matter content of the rice straw was as high as $70\%$. After immediately harvesting the paddies, using the fresh rice straw to produce RS at the same time when the dry matter content is in between $26{\sim}40\%$, the chemical composition was found to be of a good quality. Accordingly, it is recommended that when the dry matter content is in between $35{\sim}45\%$, in order to produce RS with the fine chemical composition of 'Yonchon' region, bailing should be done simultaneously with the harvesting of paddies, before the first frost appears.
Shin, J. Soon;Cho, Young-Mu;Lee, Hyo-Ho;Yoon, Sea-Hung;Park, Geun-Je;Choi, Ki-Chun
Journal of The Korean Society of Grassland and Forage Science
/
v.24
no.3
/
pp.245-252
/
2004
Experiment was carried out to find the fertilizing effects of 8 different application rates of swine compost fermented with sawdust(SCS) including Chemical fertilizer(CF) on forage yield and soil chemical characteristics of mixed pastures sown in Sep. 1993 at National Livestock Research Institute, RDA., in Suwon during low years. It was arranged in a randomized complete block design with three replicates. Dry matter yield were shown at similar among treatments except Control and $50\%$ SCS of standard amount plot. In botanical composition, the legume and weeds percentages of each treatment were increased as advancing year. The final year's legume percentage were high in line with SCS fertilizing plots($39\%{\sim}43\%$), SCS + CF plots($30\%{\sim}41\%$) and CF plot($32\%$). In productions of TDN, NE and crude protein yield, SCS or SCS($75\%$) + CF($25\%$) were nearly same comparing those of CF, respectively. Phosphate, potassium, magnesium contents and K/(Ca + Mg) except calcium contents of those SCS fertilizing plots in plant were generally high with comparing CF. Those contents were proportional according to the fertilizing amount These result indicate the possibility to substitute chemical fertilizer for SCS($75\%$, 25ton/ha) + CF, $25\%$) as manure-N 210 kg/ha, but might be considered accumulation phosphate in the soil.
Purpose: The purpose of tile study was to identify types of care needs of hospice patients and those from the family caregivers' perspective and to compare these two groups in reporting patients' care needs through Q-methodology. Methods: Twenty three Q-statements concerning care needs were selected through in-depth interviews of hospice patients. Data were collected from 20 hospice patients as well as 20 family caregivers respectively by sorting 23 Q-statements into 9 points standard. Data analysis was performed by using PC QUANL program. Results: Principal component analysis identified four types of care needs of the hospice patients. Overall, the accuracy of family caregiver reports was 48% in all types of care needs. Type 1 was named 'physical care needs type' for those whose greatest need was physical care to be free of pain and comfortable. The accuracy in Type 1 was 62.5%. Type 2 was named 'emotional care needs type' for those who would like to share love and intimacy with their family members. The accuracy in Type 2 was 20%. Type 3 was named 'spiritual care needs type' for those who would like to receive forgiveness from their God and prayers and visitation of clergy. The accuracy in Type 3 was 60%. Type 4 was named 'social care needs type' for those who would like to complete their ongoing work and to give service to others. The accuracy in Type 4 was 50%. Conclusion: There was a great difference between hospice patients and the family caregivers in reporting patients' care needs. Thus, hospice nurses need to educate family caregivers to more accurately assess patients' care needs.
In order to investigate gelation properties of Gelation Properties of ${\alpha}-lactalbumin$(${\alpha}-La$), gelling times and protein solubilities of ${\alpha}-La$ gels prepared in 0.1 M Tris-HCI buffer(pH 8.0) followed by heating at $90^{\circ}C$ for 40 minutes under different ${\alpha}-La$ concentration, NaCl, $CaCl_2$, N-ethylmaleimide(NEM), and dithiothreitol(DTT) concentration were measured. Gelling times decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but increased with increasing concentration of NEM. ${\alpha}-La$ solutions made from all treatments were gelled within 40 minutes with the exception of NEM at $20{\sim}50\;mM$. Solubilities decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but the solubility of NEM-modified gels increased with increasing concentration of NEM. As the results, solubilities In standard buffer were $10.4{\sim}51.3%$, $9.2{\sim}35.4%$, $11.1{\sim}35.0%$, $8.0{\sim}9.5%$, and $96.8{\sim}56.2%$, respectively. Solubilities in standard buffer containing 8 M urea and 0.5% SDS were higher than those in standard buffer, and were $41.8{\sim}81.3%$, $41.9{\sim}64.1%$, $43.5{\sim}69.8%$, $29.6{\sim}38.5%$, and $77.4{\sim}98.9%$, respectively. Solubilities in the presence of DTT were almost close to 100% in all conditions. These results indicates that the gelation rate and solubility are influenced by many factors, i.e. protein concentration, kind and concentration of salts, concentration of thiol reagents. The solubility of gel decreased with increasing the gelation rate.
This study was conducted to determine the pedogenesis of dense subsurface horizons (denoted either Bx or Bd) observed within closed depressions and in toeslope positions at loess-covered glacial tillplains in southern Wisconsin. Some of these dense subsurface horizons, especially those occurring within depressions, show a close morphological resemblance to fragipans elsewhere, even though the existence of fragipans has not been previously reported in southern Wisconsin. The spatial occurrence of fragipans was first examined over the landscape to characterize general soil-landscape relationships. Detailed physico-chemical and micromorphological analyses were followed to investigate the development of fragipans within a closed depression along a catenary sequence. The formation of fragipans at the study site is a result of sequential processes of physical ripening and accumulation of colloidal materials. A very coarse prismatic structure with a closely packed soil matrix was formed via physical ripening processes of loess deposited in small glacial lakes and floodplains that existed soon after the retreat of the last glacier. The physically formed dense horizons became hardened by the accumulation of colloidal materials, notably amorphous Si. The accumulation intensity of amorphous Si varies with mass balance relationships, which are governed by topography and local drainage conditions. Well-developed Bx horizons evolve at closed depressions where net accumulation of amorphous Si occurs, but the collapsed layers remain as Bd horizons at other locations where soluble Si has continuously been removed downslope or downvalley. Hydromorphic processes caused by the presence of fragipans are degrading upper parts of the prisms, resulting in the formation of an eluvial fragic horizon (Ex).
There have been many studies on the application of the reciprocal advantages of multimodality image to define accurate target volume in the Process of radiation treatment planning. For the proper use of the multimodality images, the registration works between different modality images should be performed in advance. In this study, we selected chamfer matching method and mutual information method as most popular methods in recent image registration studies considering the registration accuracy and clinical practicality. And the two registration methods were analyzed to deduce the optimal registration method according to the characteristics of images. Lung phantom of which multimodality images could be acquired was fabricated and CT, MRI and SPECT images of the phantom were used in this study. We developed the registration program which can perform the two registration methods properly and analyzed the registration results which were produced by the developed program in many different images' conditions. Although the overall accuracy of the registration in both chamfer matching method and mutual information method was acceptable, the registration errors in SPECT images which had lower resolution and in degraded images of which data were removed in some part were increased when chamfer matching method was applied. Especially in the case of degraded reference image, chamfer matching methods produce relatively large errors compared with mutual information method. Mutual information method can be estimated as more robust registration method than chamfer matching method in this study because it did not need the prerequisite works, the extraction of accurate contour points, and it produced more accurate registration results consistently regardless of the images' characteristics. The analysis of the registration methods in this study can be expected to provide useful information to the utilization of multimodality images in delineating target volume for radiation treatment planning and in many other clinical applications.
The purpose of this study is to evaluate the contribution of $^{18}$ F-FDG brain PET in the differentiating Idiopathic parkinson's diesease (IPD), progressive supranuclear palsy (PSP), and multiple system atrophy (MSA). We studied 24 patients with parkinsonism : 8 patients (mean age 67.9$\pm$10.7 y: M/F : 3/5) with IPD, 9 patients (57.9$\pm$9.2 y : M/F : 4/5) with MSA and 7 patients (67.6$\pm$4.8 y : M/F 3/4) with PSP. All patients with parkinsonism and 22 age-matched normal controls underwent $^{18}$ F FDG PET in 3D mode after the injection of 370 MBq $^{118}$ F FDG. The patients with IPD, MSh and PSP were compared with a normal control group by a two-sided t-test of SPM99 (uncorrected P<0.001, extent threshold>100 voxel). All three parkinsonism groups, showed significant hypometabolism in the cerebral neocortex compared to the normal control group. However, the three groups displayed different metabolism in the subcortical structure, brain stem, and cerebellum. In IPD, there was no significant hypometabolism in the putamen, brain stem and cerebellum. However, MSA patients showed significant hypometabolism in the striatum, pons, and cerebellum compared to the normal controls and IPD patients. In addition, PSP showed significant hypometabolism in the caudate nuclei, the thalamus, midbrain, and the cingulate gyrus compared to the normal controls, the IPD, and MSA groups (IPD vs Normal sensitivity/specificity : 75%/l00%, MSA vs Normal sensitivity/specificity :100%/87%, PSP vs Normal sensitivity/specificity : 86%/94%). Our results show that the regional metabolism of IPD, MSA, and PSP is different mainly in the striatum, thalamus, brain stem and cerebellum. An assessment of the $^{18}$ F-FDG PET scan images using SPM may be a useful adjunct to a clinical examination in making a differential diagnosis of Parkinsonism.
Youn Seon Min;Oh Young Kee;Kim Joo Heon;Park Mi Ja;Seong In Ock;Kang Kimun;Chai Gyuyong
Radiation Oncology Journal
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v.23
no.1
/
pp.51-60
/
2005
Purpose : Selective inhibition of multiple molecular targets may improve the antitumor activity of radiation. Two specific inhibitors of selective cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) were combined with radiation on the HeLa cell line. To investigate cooperative mechanism with selective COX-2 inhibitor and EGFR blocker, in vitro experiments were done. Materials and Methods : Antitumor effect was obtained by growth inhibition and apoptosis analysis by annexin V-Flous method. Radiation modulation effects were determined by the clonogenic cell survival assay. Surviving fractions at 2 Gy ($SF_2$) and dose enhancement ratio at a surviving fraction of 0.25 were evaluated. To investigate the mechanism of the modulation of radiosensitivity, the cell cycle analyses were done by flow cytometry. The bcl-2 and bax expressions were analyzed by western blot. Results : A cooperative effect were observed on the apoptosis of the HeLa ceil line when combination of the two drugs, AG 1478 and NS 398 with radiation at the lowest doses, apoptosis of $22.70\%$ compare with combination of the one drug with radiation, apoptosis of $8.49\%$. In cell cycle analysis, accumulation of cell on $G_0/G_l$ phase and decrement of S phase fraction was observed from 24 hours to 72 hours after treatment with radiation, AG 1478 and NS 398. The combination of NS 398 and AG 1478 enhanced radiosensitivity on a concentration-dependent manner in HeLa cells with dose enhancement ratios of 3.00 and $SF_2$ of 0.12 but the combination of one drug with radiation was not enhanced radlosensitivity with dose enhancement ratios of 1.12 and SF2 of 0.68 (p=0.005). The expression levels of bcl-2 and bax were reduced when combined with AG 1478 and NS 398. Conclusion : Our results indicate that the selective COX-2 inhibitor and EGFR blocker combined with radiation have potential additive or cooperative effects on radiation treatment and may act through various mechanisms including direct inhibition of tumor cell proliferation, suppression of tumor cell cycle progression and inhibition of anti-apoptotic proteins.
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