• Title/Summary/Keyword: S/C

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Application of Molecular Methods for the Identification of Acetic Acid Bacteria Isolated from Blueberries and Citrus Fruits

  • Gerard, Liliana Mabel;Davies, Cristina Veronica;Solda, Carina Alejandra;Corrado, Maria Belen;Fernandez, Maria Veronica
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.193-204
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    • 2020
  • Sixteen acetic acid bacteria (AAB) were isolated from blueberries and citric fruits of the Salto Grande region (Concordia, Entre Rios, Argentina) using enrichment techniques and plate isolation. Enrichment broths containing ethanol and acetic acid enabled maximum AAB recovery, since these components promote their growth. Biochemical tests allowed classification of the bacteria at genus level. PCR-RFLP of the 16S rRNA and PCR-RFLP of the 16S-23S rRNA intergenic spacer allowed further classification at the species level; this required treatment of the amplified products of 16S and 16S-23S ITS ribosomal genes with the following restriction enzymes: AluI, RsaI, HaeIII, MspI, TaqI, CfoI, and Tru9I. C7, C8, A80, A160, and A180 isolates were identified as Gluconobacter frateurii; C1, C2, C3, C4, C5, C6, A70, and A210 isolates as Acetobacter pasteurianus; A50 and A140 isolates as Acetobacter tropicalis; and C9 isolate as Acetobacter syzygii. The bacteria identified by 16S rRNA PCR-RFLP were validated by 16S-23S PCR-RFLP; however, the C1 isolate showed different restriction patterns during identification and validation. Partial sequencing of the 16S gene resolved the discrepancy.

Measure and Assessment of Process Capability for Nonnormal Process Data (비정규 공정 데이터에 대한 공정능력의 측도 및 평가)

  • Kim, Hong-Jun;Song, Suh-Ill
    • Proceedings of the Korean Society for Quality Management Conference
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    • 1998.11a
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    • pp.594-609
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    • 1998
  • In this dissertation, a new process capability index $C_{psk}$ is introduced for non-normal process. The Pearson curve and the Johnson curve are selected for capability index calculation and data modeling the normal-based index $C_{psk}$ is used as the model for non-normal process. A significant result of this research find that the ranking of the seven indices, $C_p,\;C_{pk},\;C_{pm},\;C^{\ast}_{pm},\;C_{pmk},\;C_s,\;C_{psk}$ in terms of sensitivity to departure of the process median from the target value T=M from the most sensitive one up to the least sensitive are $C_{psk},\;C_{s},\;C_{pmk},\;C^{\ast}_{pm},\;C_{pm},\;C_{pk},\;C_p$. i.e, By the criteria adopted for evaluation of PCI's $C_{psk}$ is the most sensitive to the departure of the process median from target and $C_p$ is least

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Effect of Sulfate on the Reaction of 3CaO.SiO2 Formation in Presence of Clinker Melt and Its Appearance in Clinker ($SO_3$가 클링커 액상이 존재하는 $3CaO.SiO_2$ 생성반응에 미치는 영향과 클링커안에서의 존재상태)

  • 정해문;한기성;최상흘
    • Journal of the Korean Ceramic Society
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    • v.30 no.1
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    • pp.7-16
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    • 1993
  • Effect of sulfate on the reactionof C3S formation in presence of clinker melt and its appearance in clinker were investigated, using (NH4)2SO4 and CaSO4 as sulfate sources. When (NH4)2SO4 and CaSO4 were added, both showed the similar results, 1.0wt% of sulfate could promoted the reaction of C3S formation, however for its content of more than 2.0wt%, the formation of C3S was prevented. Residual limit of sulfate to C3S formation is about 1.4wt%. Appearances of sulfate were C4A3l and CaSO4 in interstitial phase. For the addition of (NH4)2SO4 or CaSO4 of 20wt%~4.0wt%, C3S grains showed the hypertrophic growth. We might consider that, because sulfate reduced the surface tension and viscosity of the clinker melt, C3S crystals were precipitated below 140$0^{\circ}C$, and the crystlas of C3S were coalesced and linked in the same crystallographical direction with increasing temperature becuase of their rapid growth rate.

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Full-length Fas-associated Death Domain Protein Interacts with Short Form of Cellular FLICE Inhibitory Protein

  • Jeong, Mi-Suk;Jang, Se-Bok
    • Bulletin of the Korean Chemical Society
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    • v.27 no.1
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    • pp.87-92
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    • 2006
  • Fas-associated death domain protein (FADD) recruits and activates procaspase-8 through interactions between the death effector domains of these two proteins. Cellular FLICE-inhibitory protein (c-FLIP) was identified as a molecule with sequence homology to caspase-8. It has been postulated that c-FLIP prevents formation of the competent death-inducing signaling complex in a ligand-dependent manner, through its interaction with FADD and/or caspase-8. However, the interaction of FADD and $c-FLIP_s$ (short form) in apoptosis signaling has been controversially discussed. We show the purification and the characterization of human full-length FADD and $c-FLIP_s$ expressed in Escherichia coli. The purified FADD and $c-FLIP_s$ are shown as homogeneity, respectively, in SDS-PAGE analysis and light-scattering measurements. The folding properties of the $\alpha$-helical structure of FADD and the super-secondary structure of $c-FLIP_s$ proteins were characterized by circular dichroism spectroscopy. Furthermore, we report here a series of biochemical and biophysical data for FADD-$c-FLIP_s$ binding in vitro. The binding of both FADD and $c-FLIP_s$ proteins was detected by BIAcore biosensor, fluorescence measurement, and size-exclusion column (SEC).

AN ISOMORPHISM OF THE COUSIN COMPLEXES

  • Kim, Dae-Sig
    • Communications of the Korean Mathematical Society
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    • v.12 no.3
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    • pp.491-497
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    • 1997
  • Let $C(F, M)$ and $C(S^{-1}F, S^{-1}M)$ be Cousin complexes for a modula M and a module $S^{-1}M$ over a commutative Noetherian ring with respect to a filtration F and a filtration $S^{-1}F$ respectively. In this paper, it is shown that there is an isomorphism between the Cousin complexes $S^{-1}C(F, M)$ and $C(S^{-1}F, S^{-1}M)$.

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[r, s, t; f]-COLORING OF GRAPHS

  • Yu, Yong;Liu, Guizhen
    • Journal of the Korean Mathematical Society
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    • v.48 no.1
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    • pp.105-115
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    • 2011
  • Let f be a function which assigns a positive integer f(v) to each vertex v $\in$ V (G), let r, s and t be non-negative integers. An f-coloring of G is an edge-coloring of G such that each vertex v $\in$ V (G) has at most f(v) incident edges colored with the same color. The minimum number of colors needed to f-color G is called the f-chromatic index of G and denoted by ${\chi}'_f$(G). An [r, s, t; f]-coloring of a graph G is a mapping c from V(G) $\bigcup$ E(G) to the color set C = {0, 1, $\ldots$; k - 1} such that |c($v_i$) - c($v_j$ )| $\geq$ r for every two adjacent vertices $v_i$ and $v_j$, |c($e_i$ - c($e_j$)| $\geq$ s and ${\alpha}(v_i)$ $\leq$ f($v_i$) for all $v_i$ $\in$ V (G), ${\alpha}$ $\in$ C where ${\alpha}(v_i)$ denotes the number of ${\alpha}$-edges incident with the vertex $v_i$ and $e_i$, $e_j$ are edges which are incident with $v_i$ but colored with different colors, |c($e_i$)-c($v_j$)| $\geq$ t for all pairs of incident vertices and edges. The minimum k such that G has an [r, s, t; f]-coloring with k colors is defined as the [r, s, t; f]-chromatic number and denoted by ${\chi}_{r,s,t;f}$ (G). In this paper, we present some general bounds for [r, s, t; f]-coloring firstly. After that, we obtain some important properties under the restriction min{r, s, t} = 0 or min{r, s, t} = 1. Finally, we present some problems for further research.

An Investigation on the Magnetic Properties of Melt-Spun Fe-Pr-C Alloys (Melt-Spun Fe-Pr-C 합금의 자기적 특성 조사)

  • 장태석;조대형
    • Journal of the Korean Magnetics Society
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    • v.7 no.4
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    • pp.173-179
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    • 1997
  • Change in phases, microstructures, and magnetic properties by the variation of quench rate and heat treatment were investigated for melt-spun $Fe_{77}Pr_{15}C_8$ ribbons. The amorphization of as-spun ribbons increased as the quench rate increased. As a result, the ribbon quenched at 40 m/s was almost entirely amorphous. Similarly to cast alloys, the primary phase in crystalline ribbons quenched at 10 m/s was $\alpha$-Fe followed by the secondary $Fe_{17}Pr_2C_x$. Crystalline phases were still dominant in the ribbon spun at 20 m/s, but in this case crystallization of $Fe_{17}Pr_2C_x$ was remarkable with a little suppression of $\alpha$-Fe. At 30 m/s an amorphous phase obviously dominated in the as-spun ribbons with small fraction of crystals. Therefore, substantial amount of hard magnetic $Fe_{14}Pr_2C$ was not obtained from the as-spun state but, as in cast alloys, produced only by a solid-state transformation. Within a few minutes fine grains of $Fe_{14}Pr_2C$ were easily obtained at relatively low temperature when the degree of amorphization of as-spun ribbons was higher. The grain size of $Fe_{14}Pr_2C$ was well less than 1${\mu}{\textrm}{m}$. The ribbons quenched at 20 or 30 m/s yielded higher coercivities after heat treatment.

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Effects of Ara-C on UV and MMS-induced Excision Repair, Chromosome Aberrations, Sister Chromatid Exchanges and Replication Inhibition (자외선과 MMS에 의한 절제회복, 염색체이상, 자매염색분체 교환 및 복제억제 현상에 미치는 Ara-C의 영향)

  • Park, Kyung-Hee;Park, Sang-Dai
    • The Korean Journal of Zoology
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    • v.23 no.4
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    • pp.203-218
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    • 1980
  • Unscheduled DNA synthesis, chromosome aberrations, sister chromatid exchanges and DNA replication inhibition induced by the combined treatments with ara-C and UV-light or MMS in $HF_1$, CHO and $HelaS_3$ cells were studied, and the results obtained were as follows: (1) Ara-C was found to inhibit UV-or MMS-induced unscheduled DNA synthesis and the inhibitory effect of ara-C was more remarkable in its post-treatment. (2) Ara-C enhanced the rate of chromosome aberrations induced by MMS or UV-light. Post-treatment with ara-C exhibited the synergistic effect on MMS-induced chromosome aberrations mainly by increases of chromatid deletions. (3) Contrarily, ara-C did not increase the rate of sister chromatid exchanges, particularly in the pre-treatment with MMS, although it was found to induce sister chromatid exchanges. (4) The rate of DNA synthesis was declined immediately after are-C treatment and then recovered. The combined treatments with ara-C and UV-light or MMS showed that the initial response on replication inhibition was similar to that of ara-C, but later responses were similar to that of UV-light or MMS treated group.

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MONOTONICITY PROPERTIES OF THE GENERALIZED STRUVE FUNCTIONS

  • Ali, Rosihan M.;Mondal, Saiful R.;Nisar, Kottakkaran S.
    • Journal of the Korean Mathematical Society
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    • v.54 no.2
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    • pp.575-598
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    • 2017
  • This paper introduces and studies a generalization of the classical Struve function of order p given by $$_aS_{p,c}(x):=\sum\limits_{k=0}^{\infty}\frac{(-c)^k}{{\Gamma}(ak+p+\frac{3}{2}){\Gamma}(k+\frac{3}{2})}(\frac{x}{2})^{2k+p+1}$$. Representation formulae are derived for $_aS_{p,c}$. Further the function $_aS_{p,c}$ is shown to be a solution of an (a + 1)-order differential equation. Monotonicity and log-convexity properties for the generalized Struve function $_aS_{p,c}$ are investigated, particulary for the case c = -1. As a consequence, $Tur{\acute{a}}n$-type inequalities are established. For a = 2 and c = -1, dominant and subordinant functions are obtained for the Struve function $_2S_{p,-1}$.