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Effects of Feed Processing and Feeding Methods on Growth and Carcass Traits for Growing-Finishing Pigs

  • Chae, B.J.;Han, In K.;Kim, J.H.;Yang, C.J.;Ohh, S.J.;Rhee, Y.C.;Chung, Y.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.2
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    • pp.164-169
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    • 1997
  • The present experiment was conducted to evaluate the effects of feed processing and feeding methods on growth performance and carcass characteristics of growing-finishing pigs. A total of 72 pigs (LYD, 22.24 kg BW) were employed for a 90-d feeding trial. Treatments were 1) mash dry feeding (MD), 2) mash wet feeding (MW), 3) pellet dry feeding (PD), and 4) extruded pellet dry feeding (EPD). Corn, soybean meal and wheat bran in the basal diets were extruded before mixing and pelleting for EPD diet production. Ileal or fecal digestibility and carcass traits including lean meat percentage and weights of stomach ulcer were also examined. During the growing period, pigs fed PD showed improved (p < 0.05) average daily gain (ADG) and feed conversion (F/G) over those fed MD, whereas no significant differences in average daily feed intake (ADFI) were found among dietary treatments. Finisher pigs fed MD showed lover, but not significant, ADG and F/G than those fed MW, PD or EPD. For the overall period, pigs fed PD grew faster (p < 0.05) than those fed MD or EPD. Feed intake was different between the two feeding methods (MD vs MW), and between the two processed feeds (PD vs EPD). The digestibility of crude fat was higher (p < 0.05) in pigs fed EPD than in pigs fed mash feeds. NFE digestibility of EPD treatment was also higher (p < 0.05) than that of PD. Back fat (10th rib area) was thicker (p < 0.05) tn pigs fed MD than in pigs fed EPD. other carcass traits including incidence of esophagogastric ulcers were not different among treatments. In conclusion, pelleting appeared to bo the desirable processing methods and wet feeding could also be recommended for growing-finishing pigs.

Breast Cancer Molecular Subtypes Defined by ER/PR and HER2 Status: Association with Clinicopathologic Parameters in Ivorian Patients

  • Effi, Ahoua B;Aman, N'guiessan A;Koui, Baumaney S;Koffi, Kouadio D;Traore, Zie C;Kouyate, Mohamed
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.4
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    • pp.1973-1978
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    • 2016
  • Background: Breast cancer is a heterogeneous disease that represents a major public health problem. The immunohistochemical determination of breast cancer subtypes with regard to estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor (HER2) status can contribute to improved selection of therapy and patientcare. The purpose of this study was to determine the prevalence of the molecular breast cancer subtypes and to assess their associations with classical clinicopathologic parameters for better therapeutic decisions in women with breast cancer in the Ivory Coast. Materials and Methods: Formalin-fixed and paraffin-embedded blocks of patients diagnosed with primary breast carcinoma were subjected to immunohistochemical assay for the assessment of ER/RP and HER2 expression. The one-way analysis of variance evaluated the difference between breast cancer subtypes and mean age of patients. The Chi-square Test was used to compare standard clinicopathologic prognostic parameters with tumor subtypes. Results. Among 302 patients, 57% were premenopausal and 43% were postmenopausal. The invasive ductal carcinoma not otherwise specified (IDC NOS) (82.8%) was the most frequent histological type, and the tumor grade 2 (56%) was predominant followed by grade 3 (20.9%). The proportion of positivity of ER, PR, and HER2 was 56%, 49%, and 15.6%, respectively. Half of patients of this study (51.6%) had luminal A breast tumor type followed by TN (32.1%). Other subtypes were luminal B (10.1% ) and non-luminal HER2+ (6.3%). Conclusions. The findings of the present study are in line with the literature and should assist in management of breast cancer in our country.

CELL-MATRIX ADHESIONS OF SOFT TISSUE CELLS AROUND DENTAL IMPLANTS (임플랜트 주위 연조직세포의 세포-기질 접착)

  • Lee Suk-Won;Rhyu In-Chul;Han Chong-Hyun;Lee Jai-Bong
    • The Journal of Korean Academy of Prosthodontics
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    • v.44 no.1
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    • pp.73-84
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    • 2006
  • The importance of soft tissue response to implant abutments has become one of the major issues in current implant dentistry. To date, numerous studies have emphasized on maintaining connective tissue barriers in quantity, as well as in quality fir the long term success of dental implants. The cells mainly consisting the soft tissue around dental implants are fibroblasts and epithelial cells. The mechanism of the fibroblasts adhesions to certain substrata can be explained by the 'focal adhesion' theory. On the other hand, epithelial cells adhere tn the substratum via hemidesmosomes. The typical integrin-mediated adhesions of cells to certain matrix are called 'cell-matrix adhsions'. The focal adhesion complex of fibroblasts, in relation to the cell-matrix adhsions, consists of the extracellular matrix(ECM) such as fibronectin, the transmembrane proteins such as integrins, the intracellular cytoplasmic proteins such as vinculin, talin, and more, and the cytoskeletal structures such as filamentous actin and microtubules. The mechanosensory function of integrins and focal adhesion complexes are considered to play a major role in the cells adhesion, migration, proliferation, differentiation, division, and even apoptosis. The '3-D matrix adhesions' defined by Cukierman et al. makes a promising future for the verification of the actual process of the cell-matrix adhesions in vivo and can be applied to the field of implant dentistry in relation to obtaining strong soft tissue attachment to the implant abutments.

Reclamation of Furan Sand and Its Effect on the Cast (후란 주물사의 재생 및 그의 주물에 미치는 영향)

  • 백고길;최양진;황교현
    • Resources Recycling
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    • v.5 no.1
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    • pp.21-28
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    • 1996
  • Burmng method tn Bnidi-ied beds 101 the effectlvr reclamation of Sunn fi,uadry sand war u~ed lather tl~an rncchan1c;ll or p~~cumatiace thodq. which are co~nmonlyu scd in foundry factoricr in Kojcu In olrlcr to rcrnuvc hlc;~ns and ha~doersi ~nm foundry sand sndaces in the fluidized bcd, the lnnsl sffccuve lcmpemture mge was h00-70W. Sulbcr slab~l~liyn dca and comliresslvc streugtli oL rccla~mcd sand recyclcd by the burning luctllod wcre lalgcr dao those of rcclalmcd a n d by lllc pncumatlc mctllod. Tile energy cost IOIt he bummy mctliod was cstimalcd al SlZ0011'on oi llie iccl;~~rncds md. The coql l~ductlon in pul.chasing nzw s;od as well as the prevenllon or cnv~conmcnl.il contamlnallnn could hc hmefitcrl hy usl~lg Ihc rec1;~rnnl~oonf liisndry siod ~ecycledb y the method rleieloped in thls sludy

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A Comparative Analysis of the Illumina Truseq Synthetic Long-read Haplotyping Sequencing Platform versus the 10X Genomics Chromium Genome Sequencing Platform for Haplotype Phasing and the Identification of Single-nucleotide variants (SNVs) in Hanwoo (Korean Native Cattle) (일루미나에서 제작된 TSLRH (Truseq Synthetic Long-Read Haplotyping)와 10X Genomics에서 제작된 The Chromium Genome 시퀀싱 플랫폼을 이용하여 생산된 한우(한국 재래 소)의 반수체형 페이징 및 단일염기서열변이 비교 분석)

  • Park, Woncheoul;Srikanth, Krishnamoorthy;Park, Jong-Eun;Shin, Donghyun;Ko, Haesu;Lim, Dajeong;Cho, In-Cheol
    • Journal of Life Science
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    • v.29 no.1
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    • pp.1-8
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    • 2019
  • In Hanwoo cattle (Korean native cattle), there is a scarcity of comparative analysis papers using highdepth sequencing and haplotype phasing, particularly a comparative analysis of the Truseq Synthetic Long-Read Haplotyping sequencing platform serviced by Illumina (TSLRH) versus the Chromium Genome Sequencing platform serviced by 10X Genomics (10XG). DNA was extracted from the sperm of a Hanwoo breeding bull (ID: TN1505D2184/27214) provided by Hanwoo research canter and used for the generation of sequence data from both the sequencing platforms. We then identified SNVs using an appropriate analysis pipeline tailored for each platform. The TSLRH and 10XG platforms generated a total of 355,208,304 and 1,632,772,004 reads, respectively, corresponding to a Q30 (%) of 89.04% and 88.60%, respectively, of which 351,992,768(99.09%) and 1,526,641,824(93.50%) were successfully mapped. For the TSLRH and 10XG platforms, the mean depth of the sequencing was 13.04X and 74.3X, the longest phase block was 1,982,706 bp and 1,480,081 bp, the N50 phase block was 57,637 bp and 114,394 bp, the total number of SNVs identified was 4,534,989 and 8,496,813, and the total phased rate was 72.29% and 87.67%, respectively. Moreover, for each chromosome, we identified unique and common SNVs using both sequencing platforms. The number of SNVs was directly proportional to the length of the chromosome. Based on our results, we recommend the use of the 10XG platform for haplotype phasing and SNV identification, as it generated a longer N50 phase block, in addition to a higher mean depth, total number of reads, total number of SNVs, and phase rate, than the TSLRH platform.

Effect of soybean meal on the alcohol fermentation of sugar-alcohol-tolerant Saccharomyces cerevisiae (내당 내알콜성 Saccharomyces cerevisiae의 알콜 발효에 미치는 soybean meal의 영향)

  • Rho, Min-Jeong;Park, Keung-Ho;Paik, Un-Hwa;Yu, Ju-Hyun
    • Applied Biological Chemistry
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    • v.34 no.1
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    • pp.61-66
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    • 1991
  • In order to improve the productivity of ethanol by sugar-alcohol-tolerant Saccharomyces cerevisiae D1, the effect of addition of soybean meal on the alcohol fermentation was investigated. The addition of soybean meal led tn the increase of the ethanol productivity and viable cell concentration. Increasing the mont of soybean meal increased the number of viable cells and the consumption percentage of glucose. The water-soluble fraction of soybean meal was nearly as effective as whole-soybean meal, whereas the lipidic fraction had no positive effect. The addition of 4% soybean meal increased the rate of ethanol production regardless of the initial concentrations of glucose. The rate of glucose consumption fermenting a soybean meal supplemented medium was higher than possible in a non-supplemented medium, either in the absence or in the presence of ethanol. But the percentage of ethanol inhibition of the glucose consumption rate was identical for supplemented md unsupplemented media. The increase of final ethanol concentration could not be attributed In an increase of ethanol tolerance of yeast cells but to the satisfaction of nutritional deficiencies.

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Growth Characteristics and Adaptability of Three-Year-Old Poplar Clones in a Reclaimed Tidal Flat (간척지 시험림에서 3년생 포플러 클론의 생육특성 및 적응능력)

  • Yeo, Jin-Kie;Shin, Hanna;Kim, Hyun-Chul;Woo, Kwan-Soo
    • Journal of agriculture & life science
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    • v.45 no.5
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    • pp.17-23
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    • 2011
  • Growth characteristics and adaptability of 10 poplar clones planted at a reclaimed tidal flat were evaluated. The contents of $Na^+$, $Ca^{2+}$ and $Mg^{2+}$ were 10.0, 3.4 and 1.5 times higher, respectively than those of control although the electrical conductivity(EC) in the soil at the test plantation was low as much as 0.51 dS/m. The contents of organic matter(OM) and total nitrogen(TN) in the soil were 22.9 and 23.0 times lower than those of control. Average survival rate of 10 poplar clones showed 88% at three years after planting. Clones Eco28(Populus euramericana), Dorskamp(Populus deltoides ${\times}$ P. nigra) and I-476(Populus euramericana) showed the best survival rate of 100%. However, clones 97-19(Populus deltoides(Lux) ${\times}$ P. deltoides(Harvard)) and Suwon (Populus koreana ${\times}$ P. nigra var. italica) were relatively lower than other clones. Average height and DBH of all clones were 4.8 m and 3.6 cm, respectively. Clone Dorskamp showed the greatest height and DBH, 5.9 m and 5.0 cm, respectively. Clones 97-19 and Dorskamp showed the least defoliation by stress and visible damage by insects and diseases, whereas clones Suwon and I-476 were the most sensitive at the reclaimed tidal flat. Clone Dorskamp showed the best adaptability at the reclaimed tidal flat, but clone Suwon showed the worst based on survival rate, growth, and visible damages.

A Study on Developing A Design of Package Air Conitioner For Home use-With a Focus on Goldstar Co Package Air conditioner (가정용 PACKAGE AIR CONDITIONER 디자인 개발에 관한 연구-(주) 금성사 PAC를 중심으로-)

  • 오성진
    • Archives of design research
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    • v.13
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    • pp.153-162
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    • 1996
  • The residence space has been in the trend of being expanded with the J'l'cent economic growth Therefore PAC may be said to have [wen from business use to home US(: the progressed living standard as the goods ior Uw "ummel' S('dSOn rest'ntly though sold in Uw fast for mainly buslTw"s use. PAC has been in the demand day ;lner day and in the tn'nd on the bc,ing the necessJty of the n'sent wf',lther warming phenomenon This study is the case study nf the products development and design process of PAC which has bpcn llrivcn and developed by Gold Star Co. ,Ltd. Which .is the firm for home electric appliances representing Korea. This studies for the development and the design of the products by the firm thus emphasizing the visulizing it based on this study. For example as they being the goods for the summer season I have tried to know the physical quakity and the characters of koreans by the weather conditions and estimated the demand by setting up the target customers of the PAC as the customers' environment. And I analyzed the acts in the living room in the apartments as the living envinorment which has the direct relation with PAC and also became a help to the moulding work of PAC by analyzing the complete interior image in the living room. In the practical moulding work the Design concept was classified into the envinorment of customer, living and products to drive as the below. Firstly - New shape (Round slim) has been realized which leads the minimizing the moulding space and visul sense of opening as its being spacious. The 2nd - the high class sense of the products which are harmonious with the atmosphere of the living room by making it interiorized has been visible. The 3rd - The shape stimulating the sensitivity of the users and surface treatment are emphasized by making it be touched. The 4th - During inviting use of technology image which appears in the electronics products are attemptedby making it be high quality.king it be high quality.

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Expression Pattern of Skeletal-Muscle Protein Genes and Cloning of Parvalbumin mRNA in Dark-banded Rockfish (Sebastes inermis) (볼락(Sebastes inermis) 근육단백질 유전자의 성장단계별 발현 양상과 parvalbumin 유전자 클로닝)

  • Jang, Yo-Soon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.1-9
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    • 2011
  • Differentially Expressed Gene (DEG) was obtained from Differential Display Reverse Transcription (DDRT)-PCR using Annealing Control Primer (ACP) to search and clone genes related to developmental stages of Sebastes inermis. By using 120 ACPs, the nucleotide sequences obtained from 16 DEGs showing higher expression in 6-month-old skeletal muscle than 18-month-old ones and from 22 DEGs displaying stronger expression in 18-month-old than 6-month-old were analyzed and BLAST was conducted. The results identified that DEGs shared 69~95% homology with genes of parvalbumin (PVALB), nucleoside diphosphate kinase (NDK) B, tropomyosin (TPM), troponin I (TnI), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), muscle-type creatine kinase (CKM2), small EDRK-rich factor 2 (SERF2), adenosine monophosphate deaminase (AMPD), Trimeric intracellular cation channel type A (TRICA), Rho GTPase-activating protein 15 (ARHGAP15), S-formylglutathione hydrolase (Esterase D; ESD), heat shock protein 70 (hsp70), type 1 collagen alpha 2 (COL1A2), glutathione S-transferase, Mid1-interacting protein 1 (Mid1lip1), myosin light chain 1 (MYL1), sarcoplasmic/endoplasmic reticulum calcium ATPase 1B (SERCA1B), and ferritin heavy subunit (FTH1). Expression pattern by developmental stage of DEG14 and PVALB exhibiting strong expression in 6-month-old skeletal muscle was investigated using real time PCR. Expression was reduced as Sebastes inermis grew. Expression of PVALB gene was extremely low after 6 months of age. Expression of CKM2 showed higher expression in 18-month-old skeletal muscle than in 6-month-old muscles, and increased continuously until 4 years old, after which CKM2 expression became gradually reduced. By analysis of tissue-specific expression patterns of DEG, DEG14 was expressed mainly in skeletal muscle, liver, kidney and spleen tissues, whereas PVALB expression was expressed in skeletal muscle and kidney, but not in liver and spleen tissues. CKM2 was expressed in skeletal muscle, kidney, and spleen tissues, but not in liver tissues. PVALB gene was composed of 110 amino acids, which constituted 659 bp nucleotides. The results reported here demonstrate that the expression patterns of parvalbumin and CKM2 could be used as molecular markers for selecting fishes exhibiting fast growth.

Mapping the Research Landscape of Wastewater Treatment Wetlands: A Bibliometric Analysis and Comprehensive Review (폐수 처리 위한 습지의 연구 환경 매핑: 서지학적 분석 및 종합 검토)

  • C. C. Vispo;N. J. D. G. Reyes;H. S. Choi;M.S. Jeon;L. H. Kim
    • Journal of Wetlands Research
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    • v.25 no.2
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    • pp.145-158
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    • 2023
  • Constructed wetlands (CWs) are effective technologies for urban wastewater management, utilizing natural physico-chemical and biological processes to remove pollutants. This study employed a bibliometric analysis approach to investigate the progress and future research trends in the field of CWs. A comprehensive review of 100 most-recently published and open-access articles was performed to analyze the performance of CWs in treating wastewater. Spain, China, Italy, and the United States were among the most productive countries in terms of the number of published papers. The most frequently used keywords in publications include water quality (n=19), phytoremediation (n=13), stormwater (n=11), and phosphorus (n=11), suggesting that the efficiency of CWs in improving water quality and removal of nutrients were widely investigated. Among the different types of CWs reviewed, hybrid CWs exhibited the highest removal efficiencies for BOD (88.67%) and TSS (95.67%), whereas VSSF, and HSSF systems also showed high TSS removal efficiencies (83.25%, and 78.83% respectively). VSSF wetland displayed the highest COD removal efficiency (71.82%). Generally, physical processes (e.g., sedimentation, filtration, adsorption) and biological mechanisms (i.e., biodegradation) contributed to the high removal efficiency of TSS, BOD, and COD in CW systems. The hybrid CW system demonstrated highest TN removal efficiency (60.78%) by integrating multiple treatment processes, including aerobic and anaerobic conditions, various vegetation types, and different media configurations, which enhanced microbial activity and allowed for comprehensive nitrogen compound removal. The FWS system showed the highest TP removal efficiency (54.50%) due to combined process of settling sediment-bound phosphorus and plant uptake. Phragmites, Cyperus, Iris, and Typha were commonly used in CWs due to their superior phytoremediation capabilities. The study emphasized the potential of CWs as sustainable alternatives for wastewater management, particularly in urban areas.