• 생명과학회지
• /
• 제19권5호
• /
• pp.676-679
• /
• 2009

미생물들 사이에 존재하는 수평적 유전자 전달을 이용하여 Jurkat T cell에 대하여 새로운 항생물질을 생산하기 위해 토양박테리아 AY2000과 여러 종류의 항생물질 생산 균주인 Streptomyces griseus의 공배양을 수행하였다. MTT assay를 수행하여 세포 독성을 실험을 하였을 때 공배양 상등액은 각각 배양 하였을 때보다 높은 세포독성을 보였고 또한 48시간 배양 하였을 때 가장 높은 활성을 나타내는 것으로 나타났다. 더욱이 DAPI 염색을 하였을 때 Jurkat T cell 세포의 세포핵의 변화도 관찰 되었다. 이런 결과는 공배양 상등액에 새로운 항생물질이 생성되었음을 보여주었고 이런 방법으로 새로운 항생물질 생산에 이용되어 질수 있음을 의미한다.

• 복식문화연구
• /
• 제17권5호
• /
• pp.758-768
• /
• 2009

This study aims to analyze contemporary women's sense of fashion aesthetic by looking into the design and trend of T-panties, which have now carved out a new niche in the world's 21st century female underwear markets. By this, I'd like to reassure readers of the importance of T-panties, which has only been recently recognized, and more generally suggests the future direction of prominent T-panty design development. This study aims to analyze contemporary women's sense of fashion aesthetic by looking into the design and trend of T-panties, which have now carved out a new niche in the world's 21st century female underwear markets. By this, I'd like to reassure readers of the importance of T-panties, which has only been recently recognized, and more generally suggests the future direction of prominent T-panty design development. The domestic formativeness of all styles is divided into three types: modern feminine, mono-bosom, and utopian narcissist image. This study is baseds on the documents study.

• 한국의상디자인학회지
• /
• 제1권1호
• /
• pp.17-34
• /
• 1999

Because the United States of America has a short history, they don‘t have a special traditional culture or costumes. Besides, America is a multi-people country, they couldn’t have a consistent culture or a traditional costumes. But even they have a short history, they are trying to make a new culture. America is searching for a representative culture, which are like festival costumes, regional costumes and costumes that are form the group of people, in there country and making it into their a convention culture. This thesis is about the costumes of the U.S.A.'s famous festivals, traditional and festival costumes that are differ from every other states and the traditional costume that they are making it righ

• 한국병원경영학회지
• /
• 제14권3호
• /
• pp.1-22
• /
• 2009

Purpose : The purpose of this study was to examine the effects of nursing organizational culture and head nurse's leadership on the job satisfaction. Methods : For the purpose, a structured questionnaire was conducted. The subjects were 232 nurses who were working in the 2 general hospitals. The data were collected from Sep. 1 to Sep. 20 of 2006. Data were analyzed using descriptive statistics, one-way ANOVA, t-test, Pearson Correlation Coefficient, and multiple regression. Results : The dominant organizational culture of nursing organization was rank-oriented culture. The mean score of head nurse's transformational leadership and transactional leadership was 3.20 and 2.79 respectively. The best group of job satisfaction was affiliative-oriented culture group(3.31). The score of job satisfaction of transformational leadership's group was higher than that of transactional leadership's group. Job satisfaction was positively correlated with affiliative-oriented culture, innovative-oriented culture, task-oriented culture, and transformational leadership. Concerning of the job satisfaction, affiliative-oriented culture, rotation time and age explained 25.7%. Conclusion : The organizational culture and head nurse's leadership was correlated with job satisfaction. Especially affiliative-oriented culture and task-oriented culture influenced the job satisfaction. Based on the results, further development should be continued to develop the effective head nurse's leadership and organizational culture to improve the job satisfaction of nurses.

• 영미문화
• /
• 제9권1호
• /
• pp.1-25
• /
• 2009

This paper attempts to illuminate the significance of the myth or mythical method used in The Waste Land, which Eliot adapted from Jessie L. Weston's From Rituals to Romance and Sir James Frazer's Golden Bough. While he was composing a modern epic, James Joyce's Ulysses and Igor Stravinsky's Le Sacre du Printemps made him sure that the mythical method would be the best way to make the non-relational and chaotic modern world into a work of art. Although he accepted F. H. Bradley's epistemology that one's actual experience is non-relational, he strongly put an emphasis on 'the unified sensibility' in John Donne's poetry with which a poet changes all the dissociated material into art. He also found another effective method to give the chaotic experiences an order, and to make them modern art: the mythical method in his contemporary anthropology. With the mythical method he incorporated the various barren, horrible and ugly aspects of modern world into a new unity in The Waste Land. In addition, he embraced his contemporary anthropological theory that a primitive life described in myths is a culture just different from modern culture, and heartily employed some aspects of primitive culture to make modern poetry as well as modern culture rich and exuberant.

• 기술경영경제학회:학술대회논문집
• /
• 기술경영경제학회 2004년도 25회 하계학술발표회 논문집
• /
• pp.128-147
• /
• 2004

This paper aims at identifying the strategies for strengthening regional innovation systems. This paper is based on the objective evaluation on regional innovation systems by regional government officials who are in charge S&T promotion. This paper has two significances. First, regional government officials as manager on S&T policies, will be able to diagnose exactly their own regional innovation systems. Second, we will be able to get a complete figure on regional S&T culture throughout them. This paper argues that, in order to strengthen regional innovation systems, we must reinforce firms' technological capabilities, create and decentralize public research institutes, and diffuse the culture for appreciating S&T and innovation within region etc.

• 한국의료질향상학회지
• /
• 제28권2호
• /
• pp.2-13
• /
• 2022

Purpose:This study aimed to identify the effect of work interruption that influenced workload and perception of patient safety culture on ward nurses. Methods: Participants were 184 ward nurses, with more than 12 months of work experience, from two tertiary hospitals in S city. A descriptive correlational study design was used. Collected data were analyzed using descriptive statistics, t-test, ANOVA, Mann-Whitney U test, Kruskal-Wallis test, Pearson's correlation, and hierarchical multiple regression analysis via SPSS version 27.0. Results: The results showed that general wards nurses had a higher degree of work interruption (t=5.632, p<.001) and workload (t=3.603, p<.001) compared to comprehensive nursing care service wards nurses. More interruption in nursing work caused more burden on work (γ=.440, p<.001) and led to lesser perception of patient safety culture (γ=- .199, p=.007). Finally, the regression analysis showed that work interruption had a statistically significant relevance on nurses' workload (F=20.582, p<.001) and perception of patient safety culture (F=8.792, p<.001). Conclusion: To alleviate ward nurses' work interruption and decrease the negative effect on workload and perception of patient safety culture, it is necessary to mediate nurse staffing level and the number of assistants and utilize the environment.

• Journal of Microbiology and Biotechnology
• /
• 제19권12호
• /
• pp.1617-1619
• /
• 2009

Lactobacillus rhamnosus PL60 was tested for whether it can produce c9,t11- and t10,c12-conjugated linoleic acids (CLAs) in human. After consumption of L. rhamnosus PL60, L. rhamnosus was detected in feces 1 week after the start of intake. Analysis by gas chromatography showed that concentrations of c9,t11- and t10,c12-CLAs in serum had increased and concentrations of serum leptin had significantly decreased. Results showed that L. rhamnosus PL60 can survive in human intestines and produce CLAs in humans. This is the first report that bacteria can produce CLAs in humans.

• 한국가금학회지
• /
• 제31권4호
• /
• pp.245-253
• /
• 2004

본 연구는 육계사료 내 rPST-yeast culture의 첨가 급여가 육계의 성장성적, 도체 특성 및 골격발달 등에 미치는 영향을 규명하기 위해 수행하였다. 2일령의 Ross 육용종 수평아리 460수를 공시하여 항생제, rPST-yeast culture 및 yeast culture가 첨가되지 않은 대조구 사료와 대조구 사료에 항생제(chlorotetracycline)만을 $0.1\%$ 수준으로 첨가한 실험사료(T1), rPST-yeast culture를 각각 $0.1\%$ 및 $0.2\%$ 수준으로 첨가한 실험사료(T2 및 T3) 및 yeast culture만을 $0.2\%$수준으로 첨가한 실험사료(T4)를 각각 6주간 급여하였다. 사료섭취량과 증체량은 주 단위로 조사하였다. 실험 5주차 종료 시에 각 처리구별로 10수씩 선발하여 도살하였고, 간, 비장, 복강지방 및 가슴근육의 상대적 중량과 가슴근육의 화학적 조성을 측정하였다. 혈액 내 GOT와 GPT 수치, 총 콜레스테롤, Ca 및 P을 측정하였으며, 경골의 중량, 파쇄 강도, 회분 함량, Ca및 P 함량을 측정하였다. 증체량은 대조구에 비하여 rPST-yeast culture를 $0.1\%$ 첨가한 실험구에서 증가하는 경향을 보였으며, 사료섭취 량 및 사료요구율은 처리구간 큰 차이가 없었다. 비장 및 복강 지방의 상대적 중량은 실험구간 유의한 차이가 관찰되지 않았으나, 간과 가슴근육의 상대적 중량은 처리간에 유의한 차이가 관찰되었는데,특히 T2처리구에서 가슴근육의 상대적 중량이 대조구에 비해 유의하게 증가하는 결과가 관찰되었다(p<0.05). 가슴 근육 내 조단백질 및 조지방 함량에 처리구간에 큰 차이는 없었으나, 수분 함량은 대조구에 비해 rPST-yeast culture와 SC yeast culture 첨가 급여구에서 유의하게 증가하였다(P<0.05). 혈청 내 GOT 수치는 변화가 없었으나, GPT수치는 rPST-yeast culture 첨가 급여 후에 유의하게 감소하는 결과가 얻어졌다(P<0.05). 혈중 총 콜레스테롤, Ca 및 P 농도는 처리간에 큰 차이를 보이지 않았다. 경골중량, 파쇄강도 및 화학적 성분 조성에서도 처리간에 큰 차이는 나타나지 않았다. 본 연구의 결과로부터 사료 내 rPST-yeast culture의 첨가급여에 의해 육계에서 성장성적이 향상될 가능성이 제시되었고, 특히 가슴근육이 증가와 같은 긍정적인 효과를 기대할 수 있을 것으로 사료되었다.

• 한국양식학회지
• /
• 제20권3호
• /
• pp.168-172
• /
• 2007

해산어의 종묘생산 과정에서 로티퍼 B. rotundiformis와 코페포다 T. japonicus는 매우 대표적인 동물먹이생물이다. 그리고 이 두 종의 혼합배양 방법은 대량배양과 안정배양을 목적으로 하였을 때 매우 유용한 방법으로 널리 알려져 있다. 이 연구에서는 로티퍼 B. rotundiformis와 코페포다 T. japonicus의 혼합 배양 과정에서 미칠 수 있는 배양조건으로서 광주기가 미칠 수 있는 영향을 검토하였다. 그 결과 로티퍼 B. rotundiformis와 코페포다 T. japonicus의 혼합 배양에서는 배양 환경 조건 중 광주기 조건에 따라서 혼합배양수조내 로티퍼와 코페포다의 증식 양상이 매우 달라짐을 알 수 있었다. 로티퍼의 증식율을 비교하면 반일 광주기 조건의 최고 밀도에 비하여 전일 광주기 조건하에서 로티퍼의 최고 밀도는 200% 이상 향상되는 것을 알 수 있었다. 그러나 코페포다 T. japonicus의 증식은 12L:12D<24L:0D<0L:24D의 순으로 전일 암주기 조건의 광주기 조건하에서 가장 높은 증식이 관찰되었다. 그리고 코페포다의 증식과정을 각 발생 단계별로(nauplii, copepodites 그리고 female carrying eggs) 계수한 결과, 세 가지 광주기 조건별로 각 발생단계별(nauplius, copepodid 그리고 female carrying eggs)의 증식 양상은 매우 큰 차이를 보였다.