• Title/Summary/Keyword: Rumen Characteristics

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Effects of Supplementation of Mixed Methanogens and Rumen Cellulolytic Bacteria on Biochemical Methane Potential with Pig Slurry (양돈슬러리를 이용한 혐기소화에서 미생물 첨가가 메탄발생에 미치는 영향)

  • Kim, Ji-Ae;Yoon, Young-Man;Jeong, Kwang-Hwa;Kim, Chang-Hyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.1049-1057
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    • 2012
  • The study investigated the biochemical methane potential (BMP) assay of pig slurry supplemented with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS, M+RA+FS, and control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 60 days at $38^{\circ}C$ using anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum. In results, 5% RF and RA+FS increased total biogas up to 8.1 and 8.4%, respectively, compared with that of control (p<0.05). All 5% microbial culture supplements significantly increased methane production up to 12.1~17.9% compared with that of control (p<0.05). Total solid (TS) and volatile solid (VS) digestion efficiencies showed no relationship to the increased supplementation levels of microbial cultures. After incubation, pH values in all treatment groups ranged between 7.527 and 7.657 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that both hydrolysis and methanogenesis stages for methane production in anaerobic batch reactors were influenced by the supplemented microorganisms due to the chemical characteristics of pig slurry, but only the 5% supplementation level of all microbial culture supplements used in the experiment affected methane production.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Effect of Whole or Steam-flaked Corn Based Diet on Ruminal fermentation Characteristics In Vitro and Ruminal Metabolism in Korean Native Goat In Vivo (통옥수수 및 Steam-flaked 옥수수 기초사료가 반추위미생물 발효성상과 한국재래산양 반추위대사 특성에 미치는 영향)

  • Bae, G.S.;Bae, J.H.;Yun, S.J.;Chang, M.B.;Ko, J.Y.;Ha, Jong-K.
    • Journal of Animal Science and Technology
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    • v.44 no.6
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    • pp.757-768
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    • 2002
  • These study were conducted to determine the effects of a whole or steam-flaked corn based diet on rumen microbial fermentation in vitro and ruminal metabolism in the Korean Native Goat(KNG) in vivo. The experiments consisted of two dietary treatments: control, steam-flaked corn(SFC) based diet(80%) + rice straw mixed(20%)(SFCR); 100% whole corn based diet(WC). The first experiment was conducted to investigate the effect of whole corn on ruminal metabolism in vitro for 0 to 48 h. pH values were optimally maintained during incubation time, and were not significantly different between treatments. Gas production of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration tended to increase for WC, but not significantly different between treatments. The mean value of total volatile fatty acid concentration of WC was significantly lower than SFCR(p<0.01), but SFCR and WC linearly increased as the time of incubation approached 48 h. Mean value of acetate concentration of SFCR was significantly higher than WC(p<0.01). Propionate concentration of WC for the total incubation time was significantly higher than SFCR(p<0.01). The digestibility of dry matter was not significantly different between treatments, but SFCR was somewhat higher than WC. The second experiment was conducted to effect of whole shelled corn based diet on rumen metabolism in KNG. pH values tended to decrease through all treatments. There was not a significantly difference between treatments. Microbial protein yield of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration of WC was significantly (p<0.01) higher than SFCR. Total VFA and propionate concentration of WC was significantly higher than SFCR(p<0.01), but acetate concentrate of WC was not significantly higher than SFCR. The mean value of total lactate concentration was significantly(p<0.01) different but the value of SFCR and WC were lower than the average concentration of acidosis. In sacco DM disappearance rate of SFC was significantly(p<0.01) higher than WC.

Effect of Feeding Monensin on the Body Gain and Ruminal Parameters of Han-Woo Cattle (Monensin의 급여가 한우의 증체 및 제1위액의 성상에 미치는 영향)

  • Lee, S.K.;Jung, K.K.
    • Korean Journal of Agricultural Science
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    • v.27 no.1
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    • pp.25-32
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    • 2000
  • This experiment was conducted to investigate the effect of feeding monensin on the growth performance and ruminal fermentation characteristics of Han-Woo cattle. Seventy two uncastrated Han-Woo male cattle(BW 267 kg) were randomly allotted to 0, 22, and 33 ppm monensin treatments, three replicates per treatment and eight heads per replicate. Animals were kept in an open barn for an 140-d feeding trial, Concentrates containing different levels of monensin and rice straw cut in 15cm length were fed ad libitum separately. The results obtained from this study were summarized as follows. 1. No significant difference was found in daily gain by monensin feeding. 2. Monensin did not affect the total feed (concentrate + roughage) intake: however, as the monensin level increased, the total feed intake tended to decrease, resulting in 5 % reduction in 33 ppm monensin treatment. 3. Although no significant difference was found among three treatments, 22 and 33 ppm monensin improved the feed efficiency(total feed/gain) by 5.2 % and 5.1 %, respectively, as compared to the 0 ppm monensin treatment. 4. Monensin did not affect the concentrations of ruminal total VFA and acetic acid consistently. Although not significant, monensin feeding of 22 and 33 ppm caused marked increase in ruminal propionic acid concentration, 13.8 % and 19.3 %, respectively. Ruminal butyric acid concentration decreased as monensin level increased. Monensin feeding, regardless of level, decreased the A/P ratio by 12.5 %. In conclusiuon, monensin feeding increased the propionic acid concentration, and decreased the butyric acid concentration and A/P ratio in the rumen. Animals fed monensin consumed less feed, causing the improvement in feed efficiency. Thus, monensin appeared to be a useful feed additive, directing the rumen fermentation in a more productive way. Feed efficiency improved similarly both in 22 and 33 ppm monensin treatments, indicating that 22 ppm might be good enough rather than the 33 ppm monensin level.

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Effect of Lactic acid bacteria and Enzyme Supplementation on Fermentative Patterns of Ensiling Silages, Their In vitro Ruminal Fermentation, and Digestibility (젖산균과 효소제 처리에 의한 동계사료작물 발효성상, In vitro 반추위 발효 및 소화율에 미치는 영향 연구)

  • Lee, A-Leum;Shin, Su-Jin;Yang, Jinho;Cho, Sangbuem;Choi, Nag-Jin
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.1
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    • pp.7-14
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    • 2016
  • The objective of this study was to determine the effect of bacterial inoculation (Lactobacillus plantarum or combo inoculant mixed with Lactobacillus plantarum and Lactobacillus buchneri) and addition of fibrolytic enzyme on chemical compositions and fermentation characteristics of whole crop barley (WCB) and triticale (TRT) silage, their ruminal in vitro fermentation, and digestibility. In TRT silage, enzyme addition significantly (p<0.01) decreased NDF content compared to no enzyme addition treatment. Organic acids such as lactate and acetate contents in WCB and TRT silages were significantly (p<0.01) higher compared to those in the control. Particularly, lactate content was the highest in L. plantarum treatment. Fibrolytic enzyme treatment on both silages had relatively higher lactic acid bacteria content, while mold content was lower in both treatments compared to that in the control. In vitro dry matter digestibility was generally improved in WCB silages. It was higher (p<0.01) in TRT with mixed treatment of L. plantarum, L. buchneri, and enzyme compared to others. In vitro ruminal acetate production was relatively higher in treatments with both enzyme and inoculant additions compared to that in the control. Therefore, the quality of silage and rumen fermentation could be improved by inoculants (L. plantarum and L. buchneri) regardless whether whole crop barley (WCB) or triticale (TRT) silage was used. Although it was found that fibrolytic enzyme addition to both silages had various quality and rumen fermentation values, further study is needed.

Evaluation of Growth Characteristics and Feed Value of Korean Native Sweet Sorghum as Forage Crop (사료작물로서 국내 재래종 단수수의 생육 특징 및 사료가치 평가)

  • Hyun-Sik Choi;Ha Guyn Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.232-239
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    • 2023
  • This study was to evaluate the values of Korean native sweet sorghum as a new feed crop for ruminants. Sweet sorghum was the Muan native species (Bioenergy Crop Research Center, National Institute of Crop Science), and cultivated from May to October 2021 at Sangji University (Wonju-si, Gangwon-do, Korea). There were a non-treated group (Con), a recommended amount treatment (RD) and a treatment with double the recommended amount (Double RD) by an oil cake fertilizer. Plant height was measured at weekly intervals for 12 weeks after planting sweet sorghum seedlings, and was a significant difference in the order of Double RD, followed by RD and Con in 7 weeks (p<0.05). Feed values and sugar contents were measured in 7, 9, and 11 weeks. Crude protein of Double RD was higher than that of the other treatments in 7 and 9 weeks (p<0.05). Crude fat was higher at Double RD than the other one in 9 weeks (p<0.05). ADF and NDF of Double RD were higher than the other one (p<0.05). When it was compared to corn and sudangrass hybrids grown on farms, Crude protein was lower in sweet sorghum than other crops (p<0.05), and crude fat was higher in sweet sorghum than corn (p<0.05). Crude fiber, ADF and NDF were higher in sweet sorghum compared to corn and sudangrass (p<0.05). The sugar contents of sweet sorghum were 4.07 ± 0.12~7.63 ± 0.21 brix, and showed higher than corn and sudangrass hybrid (p<0.05). The rumen in situ digestibility of sweet sorghum was 30.73~38.13% at the 9th and 11th weeks, and showed higher than that of corn and sudangrass hybrids (p<0.05). Therefore, it is considered that Korean native sweet sorghum has sufficient value as a new forage crop for ruminants, and good value as yield, nutrients and digestibility, when the grass height is 273.33~332.50 cm.

Nutritional value and in situ degradability of oak wood roughage and its feeding effects on growth performance and behavior of Hanwoo steers during the early fattening period

  • Ju, Ye Ri;Baek, Youl Chang;Jang, Sun Sik;Oh, Young Kyoon;Lee, Sung Suk;Kim, Yong Sik;Park, Keun Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.6
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    • pp.930-940
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    • 2020
  • Objective: This study was conducted to evaluate changes in nutritional value and in situ dry matter (DM) degradability of oak and pine wood before and after steam-digestion process (60 min/160℃/6 atm) and feeding effect of the oak roughage on performance and behavior of Hanwoo steers. Methods: Chemical composition and tannin concentration were analyzed for oak and pine trees before and after the pretreatment. In situ DM and effective degradability of these samples were assessed using a nylon bag method. In vivo trial was performed to estimate animal performance and behavior, using steers fed total mixed ration (TMR) diets containing 0% (control), 25% (OR-25), and 50% (OR-50) of the oak roughage. Eighteen steers were allocated into nine pens (2 steers/pen, 3 pens/treatment) for 52 days according to body weight (BW) and age. Results: By the steam-digestion treatment, the neutral detergent-insoluble fiber was decreased from 86.5% to 71.5% for oak and from 92.4% to 80.5% for pine, thereby increasing non-fiber carbohydrate. In situ DM degradability of treated oak reached 38% at 72 h, whereas that of untreated oak was only 11.9%. The 0 h degradability of the treated pine increased from 5.9% to 12.1%, but the degradability was unchanged thereafter. Animal performance including BW, average daily gain, DM intake, and feed conversion ratio was not different among control and oak treatments. No differences were detected in animal behavior such as lying, standing, rumination, drinking, and eating, except walking. Walking was higher in control than oak treatments with numerically higher eating and lower lying times, probably due to bulkier characteristics of rice straw in the diet. Conclusion: This study demonstrates that the oak roughage can be substituted for 50% of total forage or 100% of rice straw in TMR diets at early fattening stage of Hanwoo steers.

Effects of Supplementing Brown Seaweed By-products in the Diet of Holstein Cows during Transition on Ruminal Fermentation, Growth Performance and Endocrine Responses

  • Hong, Z.S.;Kim, E.J.;Jin, Y.C.;Lee, J.S.;Choi, Y.J.;Lee, H.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.9
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    • pp.1296-1302
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    • 2015
  • This study was conducted to examine the effects of supplementing brown seaweed by-products (BSB) in the diet of ruminants on ruminal fermentation characteristics, growth performance, endocrine response, and milk production in Holstein cows. In Experiment 1, the effects of different levels (0%, 2%, and 4% of basal diet as Control, 2% BSB, 4% BSB, respectively) of BSB were evaluated at 3, 6, 9, 12, and 24 h in vitro batch culture rumen fermentation. The pH tended to be higher for the higher level of BSB supplementation, with the pH at 12 h being significantly higher (p<0.05) than that of the control. The concentration of ammonia nitrogen was lower at 3, 9, 12, and 24 h incubation (p<0.05) compared with the control, and tended to be low at other incubation times. Volatile fatty acid concentration appeared to be minimally changed while lower values were observed with 4% BSB treatment at 24 h (p<0.05). In Experiment 2, effects of levels (0%, 2%, and 4%) of BSB on growth performance, endocrine responses and milk production were studied with Holstein dairy cows during transition. Dry matter intake, daily gain and feed efficiency were not affected by BSB supplementation. The concentration of plasma estrogen for the control, 2% BSB and 4% BSB after three months of pregnancy were 55.7, 94.1, and 72.3 pg/mL, respectively (p = 0.08). Although the differences of progesterone levels between BSB treatments and the control were minimal, the concentration in 4% BSB treatment increased to 157.7% compared with the initial level of the study. Triiodothyronine and thyroxine levels were also higher after both three months and eight months of pregnancy than the initial level at the beginning of the study. In addition, BSB treatments during one month after delivery did not affect daily milk yield and composition. In conclusion, the present results indicate that supplementation of BSB did not compromise ruminal fermentation, and animal performance at lower levels and hence may have potential to be used as a safe feed ingredient in dairy cows.

Evaluation of Different Yeast Species for Improving In vitro Fermentation of Cereal Straws

  • Wang, Zuo;He, Zhixiong;Beauchemin, Karen A.;Tang, Shaoxun;Zhou, Chuanshe;Han, Xuefeng;Wang, Min;Kang, Jinhe;Odongo, Nicholas E.;Tan, Zhiliang
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.2
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    • pp.230-240
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    • 2016
  • Information on the effects of different yeast species on ruminal fermentation is limited. This experiment was conducted in a $3{\times}4$ factorial arrangement to explore and compare the effects of addition of three different live yeast species (Candida utilis 1314, Saccharomyces cerevisiae 1355, and Candida tropicalis 1254) at four doses (0, $0.25{\times}10^7$, $0.50{\times}10^7$, and $0.75{\times}10^7$ colony-forming unit [cfu]) on in vitro gas production kinetics, fiber degradation, methane production and ruminal fermentation characteristics of maize stover, and rice straw by mixed rumen microorganisms in dairy cows. The maximum gas production (Vf), dry matter disappearance (IVDMD), neutral detergent fiber disappearance (IVNDFD), and methane production in C. utilis group were less (p<0.01) than other two live yeast supplemented groups. The inclusion of S. cerevisiae reduced (p<0.01) the concentrations of ammonia nitrogen ($NH_3$-N), isobutyrate, and isovalerate compared to the other two yeast groups. C. tropicalis addition generally enhanced (p<0.05) IVDMD and IVNDFD. The $NH_3$-N concentration and $CH_4$ production were increased (p<0.05) by the addition of S. cerevisiae and C. tropicalis compared with the control. Supplementation of three yeast species decreased (p<0.05) or numerically decreased the ratio of acetate to propionate. The current results indicate that C. tropicalis is more preferred as yeast culture supplements, and its optimal dose should be $0.25{\times}10^7$ cfu/500 mg substrates in vitro.

Metabolisable Energy, In situ Rumen Degradation and In vitro Fermentation Characteristics of Linted Cottonseed Hulls, Delinted Cottonseed Hulls and Cottonseed Linter Residue

  • Bo, Y.K.;Yang, H.J.;Wang, W.X.;Liu, H.;Wang, G.Q.;Yu, X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.240-247
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    • 2012
  • Dietary supplementation with conventional linted cottonseed hulls (LCSH) is a common practice in livestock production all over the world. However, supplementation with mechanically delinted cottonseed hulls (DCSH) and cottonseed linter residue (CLR) is uncommon. Cottonseed by-products, including LCSH, DCSH and CLR, were assessed by chemical analysis, an in situ nylon bag technique, an in vitro cumulative gas production technique and in vitro enzyme procedure. The crude protein (CP) content of CLR (302 g/kg dry matter (DM)) was approximately 3 times that of LCSH and 5 times that of DCSH. The crude fat content was approximately 3 times higher in CLR (269 g/kg DM) than in LCSH and 4 times higher than in DCSH. Neutral detergent fibre (311 g/kg DM) and acid detergent fibre (243 g/kg DM) contents of CLR were less than half those of DCSH or LCSH. Metabolisable energy, estimated by in vitro gas production and chemical analyses, ranked as follows: CLR (12.69 kJ/kg DM)>LCSH (7.32 kJ/kg DM)>DCSH (5.82 kJ/kg DM). The in situ degradation trial showed that the highest values of effective degradability of DM and CP were obtained for CLR (p<0.05). The in vitro disappearance of ruminal DM ranked as follows: CLR>LCSH>DCSH (p<0.05). The lowest digestibility was observed for DCSH with a two-step in vitro digestion procedure (p<0.05). The potential gas production in the batch cultures did not differ for any of the three cottonseed by-product feeds. The highest concentration of total volatile fatty acids was observed in CLR after a 72 h incubation (p<0.05). The molar portions of methane were similar between all three treatments, with an average gas production of 22% (molar). The CLR contained a higher level of CP than did LCSH and DCSH, and CLR fermentation produced more propionate. The DCSH and LCSH had more NDF and ADF, which fermented into greater amounts of acetate.