• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.383-390
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• 1998

The purpose of this study was to evaluate the effect of new materials with forest topsoil for hydroseeding measures that is environmentally and ecologically stable revegetation measures on rock cut-slopes. Field hydroseeding experiment was used with a completely randomized design at highway rock cut-slopes in April, 1997. Results obtained in this study were summarized as follows: Particle size distribution of 3 mixed-soil materials that consisted of forest topsoil, decomposed granite soil, compost, sludge, and bottom ash did not show a significant difference. As appending the amount of forest topsoil, soil bulk density was increased. Soil hardness was slightly increased in early period, and then decreased with the flourishing of plants. The number of individuals increased, more than $3,000seedlings/m^2$, after 1 month, and it was decreased as time passes because of competition between the seeded species and the naturally emerged species. In addition to the seeded species, seeding plot has more than 6 species (Rubus crataegifolius, Eleusine indica, Erigeron canadensis, Lycopersicon esculentum var. cerasiforme, etc.) per $m^2$ were naturally appeared in the first year. From the viewpoint of species diversity promotion, the capability of using forest topsoil as seed bank sources was high. In order to apply in the field, the investigation and analysis of topsoil availability (quantity and quality of seed source, soil texture and organic composition) should be carried out before-hand.

• The Korean Journal of Ecology
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• v.27 no.6 s.122
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• pp.335-345
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• 2004

This study was carried out to on liana dominating at the vicinity of Onsan Industrial Complex declining P. thunbergii foersts. It was surveyed 16 species, 30 species and 50 species at upper, middle and understory, respectively. It was dominant P. thunbergii at upperstory, and P. densiflora, P. thunbergii, Quercus dentata and Q. serrata at middlestory, and Smilax china, Paederia scandens, Symplocos chinensis var. sinuata, Styrax japonica, Lonicera japonica and Q. serrata etc. at understory. It was the highest importance value(13.2) of S. china among all liana, and in order of L. japonica (11.7), P. scandens (11.5) and Cocculus trilobus (7.7). Number of species, Species diversity, Maximum species diversity, Evenness and Dominance of woody plants at upperstory and middlestory by each survey site were higher in forest areas than industrial complex, but those of understory highly showed at industrial complex, and ratio of liana at understory was high. It showed total 50 species at herbaceous plants, and was high I.V. of Pteridium aquilinum var. latiusculum, Spodiopogon cotulifer, Commerelina commuris, Phytolacca americana, Rubus parvifolius, Miscanthussinensis var. purpurascens and Calamagrostis arundinacea, etc.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.55-55
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• 2015

Rust is one of the most destructive diseases on economically important plants such as agricultural and horticultural crops, as well as forest trees [1]. Chemical treatment is the most effective means to control rust, but use of the chemical fungicides involves inevitable risks to human health and environment [2]. Unfortunately, biocontrol is currently impracticable for rust disease management [3]. It is necessary to exploit biocontrol agents to help prevent rust diseases. As a fundamental research for future development of biocontrol agents for rusts, biodiversity of hyperparasites occurring on rust fungi was investigated. During 2006-2010, 197 fungal isolates of the rust hyperparasites were collected and isolated from various combinations of mycohosts and plant hosts in many regions of Korea. Based on morphological and molecular data, they were identified as 8 genera and 12 species. Besides, phylogenetic relationships between the hyperparasites and related taxa were inferred. A total of 114 isolates of Pseudovirgaria were obtained from rust pustules of Phragmidium spp. and Pucciniastrum agrimoniae infecting rosaceous plants. Phylogenetic analysis using multigene sequences revealed a high level of genetic variability among many isolates of Pseudovirgaria and close correlation between the isolates and mycohosts. Only two species of Pseudovirgaria, P. hyperparasitica and P. grisea are often difficult to distinguish by their morphological similarity, but on the molecular basis they were clearly differentiated from each other. There had been no previous record of P. grisea outside Europe, but the present study has proved its presence in Korea. Among six distinct groups (five of P. hyperparasitica and one of P. grisea) within the Pseudovirgaria isolates, each lineage of P. hyperparasitica was closely associated with specific mycohosts and thus might have cospeciated with their mycohosts, which probably led to coevolution. Although P. grisea possesses a host preference for Phragmidium species occurring on Rubus, it was not specific for a mycohost. P. grisea seems to evolve in the direction of having a broad mycohost range. Seventeen isolates of Verticillium-like fungi were isolated from rust sori. Based on morphological data and DNA sequence analysis, the isolates were identified as three Lecanicillium species, viz. L. attenuatum, Lecanicillium sp. 1, Lecanicillium sp. 2, and V. epiphytum. The unidenified two species of Lecanicillium appear to be previously unknown taxa. Sixty-six isolates of miscellaneous hyphomycetes belonging to 6 species of 5 genera were obtained from pustules of rust fungi. On the basis of morphological and molecular analyses, the miscellaneous hyphomycetes growing on rusts were identified as Acrodontium crateriforme, Cladophialophora pucciniophila, Cladosporium cladosporioides, Phacellium vossianum, Ramularia coleosporii, and R. uredinicola.

• The Korean Journal of Food And Nutrition
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• v.28 no.6
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• pp.1098-1106
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• 2015

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

• KSBB Journal
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• v.18 no.4
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• pp.282-288
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• 2003

For the screening of anti-inflammation and antioxidative activities, ethanolic extract of 40 species of traditional herbal medicines were examined their hyaluronidase inhibitory effect and radical scavenging activity in vitro. From the result of the hyaluronidase inhibitory activity using a Morgan-Elson assay, Astragali Radix, Eucommia Cortex, Schizandrae Fructus, Scutellaria Radix, Acanthopanacis Cortex, Chaenomelis Fructus, Amomum xanthioides Wallich and Moutan Radicis Cortex showed more than 50% hyaluronidase inhibitory effects at the concentration of 10 mg/mL. In the various solvent fractions (n-hexane, chloroform, ethyl acetate, water) prepared from ethanolic extracts, the ethyl acetate fraction of all extracts tested showed strong activity. Antioxidative activity was evaluated by assaying electron-donating ability to DPPH free radical and scavenging of hydroxyl radical (ㆍOH) generated through Fenton reaction, respectively. Rubus coreanus Miq, Moutan Radicis Cortex, Paeoniae Radix Alba, Plantaginis Semen and Sorbus commixta Hedl. showed high activity more than 90%, yet similar activity to $\alpha$-tocopherol and BHA at the concentration of 1 mg/mL in electron donating activity. The scavenging effects of ethanolic extracts on hydroxyl radical were investigated using a 2-deoxyribose oxidation method and tested all extracts showed significant radical scavenging activity. The experiment was also performed to examine whether herbal medicines having significant lipid peroxidation inhibitory activity, Schizandrae-Fructus is the strongest inhibitory activity in both linoleic acid and liposome peroxidation.

• Korean Journal of Environment and Ecology
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• v.24 no.1
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• pp.14-25
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• 2010

This study was carried out to investigate the soil salinity and salt spray drift of the indigenous windbreak trees, and its main purpose was to provide basic data for the selection of salt-tolerant trees in the saline coastal region in the South Sea of Korea. The soil salinity($EC_{1:5}$)was $0.18dSm^{-1}$, which was an average degree of the whole areas of investigation whose salinity degree ranged from $0.05dSm^{-1}$ to $0.58dSm^{-1}$. The level of soil salinity gradually decreased as it moved farther inland, except the belt I. The level of decreasing soil salinity was found to be in the following order: belt II, belt III, belt I, belt IV. The degree of soil salinity was $EC_{1:5}$ $0.22dSm_{1:5}$, $0.22dSm_{1:5}$ $0.19dSm^{-1}$ and $0.13dSm^{-1}$ respectively. The total 110 taxa, which consisted of 45 families, 74 genus, 101 species, and 9 varieties, were found to be tolerant to both soil salinity and salt spray drift. The trees that grow in the highest degree of salinity($EC_{1:5}$ $0.50dSm^{-1}$)were Parthenocissus tricuspidata(Siebold & Zucc.), Planch and Lonicera japonica Thunb. The next group of trees that grow in the high degree of salinity ranging from $EC_{1:5}$ 0.41 to $0.50dSm^{-1}$ was Cudrania tricuspidata(Carr.) Bureau ex Lavall$\acute{e}$e, Rubus parvifolius L., Zanthoxylum schinifolium(Siebold & Zucc.), Hedera rhombea(Miq.) Bean., Robinia pseudoacacia L., Quercus serrata Thunb., Callicarpa dichotoma(Lour.) K. Koch, and so on. The woody species which grew in the entire belts were Pueraria lobata(Willd.) Ohwi and Vitis flexuosa Thunb., and Vitex rotundifolia L. f. which was known to be highly tolerant to salt spray drift was found only in belt I. The woody species with high important value(IV) were Zelkova serrata(Thunb.) Makino., Celtis sinensis Pers., Koelreuteria paniculata Laxmann, Mallotusjaponicus(Thunb.) Muell. Arg., Trachelospermum asiaticum(Siebold & Zucc.) NAKAI, and Pueraria lobata(Willd.) Ohwi. These species were classified as native windbreak trees that are comparatively more tolerant to salt spray drift than other kinds.

• Korean Journal of Food Science and Technology
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• v.46 no.2
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• pp.198-204
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• 2014

Ultraviolet B (UV-B) irradiation is a negative factor that induces skin damage, inflammation, and aging. UVB irradiation induces the inflammatory response through interleukin (IL)-6 and IL-8 expression in keratinocytes. In addition, it induces the production of reactive oxygen species (ROS) and the activation of matrix metalloproteinase-1 (MMP-1), which plays an important role in collagen 1 degradation in the extracellular matrix. We investigated the antiaging effects of five kinds of berry in human skin keratinocyte (HaCaT) cells using juice of black raspberry (Rubus occidentalis), blueberry wild (Vacciniun angustifolium) and cultivar (Vacciniun corymbosum), black chokeberry (Aronia melanocarpa (Michx.) Elliott), and mulberry (Morus abla). HaCaT cells irradiated with UV-B exhibited increased ROS generation, as well as IL-6, IL-8, and MMP-1 gene expression, when compared to the control cells that were not irradiated with UV-B. However, pre-treatment of berry juice before UV-B irradiation significantly down-regulated the UV-B-induced ROS generation and inflammatory cytokine and MMP-1 expression. The results suggest that all berries have anti-aging effects including lowering inflammatory cytokine levels, ROS generation, and MMP-1 expression in HaCaT cells during UV-B irradiation.

• Korean journal of applied entomology
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• v.24 no.4 s.65
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• pp.183-190
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• 1986

Identification of fruit sucking moths, their larval host plant, and degree of damages to fruits were investigated in $1982{\sim}'83$ at two orchards in Cheonnam province. In this study, 4 families 30 species were collected as fruit sucking moths and among them 10 species are newly recorded in Korea as fruit sucking moth: Catocala electa(B.), Spirama helicina(H.), Erebus ephesperis(H.), Parallelia stuposa(F.), Simplicia niphona(B.), Marumba gaschkewitschii(B. et G.), Agrius convolvuli(L.), Thyatira batis (L.), and Spilosoma niveum(M.). Larval host plants of fruit sucking moths in the vicinity of the fruit orchard include Cocculus trilobus D., Albizzia julibrissin D., Pterocarya rhoifolia S., Jaglans mandshurica M., Solanum melongena L., Brassia campestris S., Rubus idaeus C., Thalictrum aquilegifol L., and Rhus succedanea L. As the damage on different varieties of the main fruit trees, plum was orderly Santa Rosa>Beauty, Apple was Chook>Hong Og, Peach was Choseng Baeg Do>Hwang Do>Baeg Do, and Grape was Campbell Early>Neo Mascat>Golden Queen. Seasonal occurrence of main fruit sucking moth Spirama retorta(C.) showed peak 3 times in early-July, late-July, early-September. Oraesia emerginata(F.) showed peak 3 times in late-July, early-August, early- September and Lagoptera juno(D.) showed peak only in late-July and Adrias tyrannus amurensis(S.) showed peak only in mid-July. Fruit sucking moths were immigrated from p.m. 6 to a.m. 2, with peak in p.m. 10 to p.m. 12.

• Korean Journal of Food Science and Technology
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• v.46 no.3
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• pp.384-389
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• 2014

The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (${\gamma}$-B201) as well as the protective effect of ${\gamma}$-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that ${\gamma}$-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide ($H_2O_2$)-treated HepG2 cells. Furthermore, treatment with ${\gamma}$-B201 attenuated DNA damage in $H_2O_2$-treated HepG2 cells and treatment with ${\gamma}$-B201 restored the activity of superoxide dismutase and catalase in $H_2O_2$-treated HepG2 cells. In conclusion, the present study suggests that ${\gamma}$-B201 blackberry extract can exert a significant cytoprotective effect against $H_2O_2$-induced cell damage.