• The Plant Pathology Journal
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• v.16 no.1
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• pp.48-51
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• 2000

Citrus tristeza virus (CTV) was identified form CTV-infected early satsuma mandarin (Citus unshiu) and yuzu (C.junos) by RT-PCR. The total RNAs were isolated from citrus bark and seaf tissues infected with CTV and reverse transcription was followed with primers designed for amplifying CTV coat protein gene. DNA fragments 738 bp were amplified by RT-PCR and these products were colned for sequence analysis. Based on the sequence analysis, this PCR product has 97% sequence homology to CTV (T-385) CP gene isolated from USA. RT-PCR assay for CTV detection was more sensitivity than ELISA assay which was done with anti-CTV CP antibody. This is the frist report about CTV identification in Cheju island Korea.

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.187-190
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• 1996

백합으로부터 total RNA를 분리하여 LSV 외피단백질 유전자의 551 bp에 해당하는 특정 염기서열을 증폭할 수 있는 primer로 RT-PCR를 수행하였다. 그 결과 Lilium oriental hybrid cvs. Miani, Marco Polo, Casablanca, Le Reve 품종에서 551 bp의 DNA 절편이 증폭되었고 이 절편의 염기서열을 분석한 결과 LSV외피단백질 유전자의 일부임을 확인할 수 있었다. 그러므로 RT-PCR 방법으로, 실험에 사용하 s4품종 모두 LSV에 감염되어 있음을 알 수 있었다.

• 한국IT서비스학회:학술대회논문집
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• 2009.11a
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• pp.419-423
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• 2009

u-City 상에는 다양한 종류의 임베디드 기기가 존재하며, 최근 이들 간의 다양한 데이터 처리 및 기기 간 통신의 상호운영성에 대한 문제가 이슈화 되고 있다. RT-EdSB (Real-time Embedded Service Bus)는 ESB와 DDS 그리고 CEP를 기반으로 하는 프레임워크로 임베디드 기기에서 발생하는 다양한 수많은 데이터의 실시간으로 처리하면서 동시에 임베디드 기기간의 통신의 상호운영성을 보장하는 것을 목표로 하고 있다. 본 논문에서는 RT-EdSB 프레임워크 아키텍처를 제안하고 또한 u-City에서의 RT-EdSB의 활용 방안을 제시하고 있다.

• Journal of ILASS-Korea
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• v.22 no.1
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• pp.29-35
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• 2017

This paper describes the effects of spray breakup model constants on spray and combustion characteristics in single cylinder compression engine. KIVA-3V code coupled with a CHEMKIN chemistry solver was used for numerical analysis. In this study, spray simulations and combustion simulations are studied simultaneously. Spray simulation was conducted in constant volume to reduce the effects of air-flow as swirl or tumble. The model validation was conducted and there are little difference between experiments and simulation, this differences were reasonable. In spray simulation, the effects of model constants on spray tip penetration, spray patter and SMD were studied. Furthermore, the analysis of effects of breakup variables on combustion and emissions characteristics was conducted. The results show the KH-RT breakup model constants affects spray and combustion characteristics strongly. Increasing KH model variable (B1) and RT model constants ($C_{\tau}$, $C_{RT}$) induced slower breakup time.

• Journal of information and communication convergence engineering
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• v.5 no.1
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• pp.29-34
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• 2007

This paper proposes a MAC protocol for real-time VBR (rt-VBR) services in wireless ATM networks. The proposed protocol is characterized by a contention-based mechanism of the reservation request, a contention-free polling scheme for transferring the dynamic parameters, and a priority scheme of the slot allocation. The design objective of the proposed protocol is to guarantee the real-time constraint of rt-VBR traffic. The scheduling algorithm uses a priority scheme based on the maximum cell transfer delay parameter. The wireless terminal establishes an rt-VBR connection to the base station with a contention-based scheme. The base station scheduler allocates a dynamic parameter minislot to the wireless terminal for transferring the residual lifetime and the number of requesting slots as the dynamic parameters. Based on the received dynamic parameters, the scheduler allocates the uplink slots to the wireless terminal with the most stringent delay requirement. The simulation results show that the proposed protocol can guarantee the delay constraint of rt-VBR services along with its cell loss rate significantly reduced.

• Radiation Oncology Journal
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• v.35 no.4
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• pp.380-384
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• 2017

Massive chylous leakage is a rare postoperative complication that can prolong hospital stay and cause secondary morbidities. Conservative management strategies are the treatment of choice; however, radiation therapy (RT) can be used as an alternative for cases that are refractory to conservative treatment. Herein, we report a 69-year-old female patient who suffered from massive chylous leakage after subtotal gastrectomy. Due to persistent massive chylous leakage, she was scheduled to undergo low-dose RT. Radiation was delivered with a daily dose of 1 Gy, using an anterior-posterior and posterior-anterior beam arrangement. The clinical target volume encompassed the entire lymph node area of the D2 dissection. RT was completed at the total dose of 8 Gy because the amount of chylous leakage declined rapidly. Percutaneous drainage tube was removed after 3 days of RT. The patient did not complain of any symptoms related to massive chylous leakage 2 years after the completion of RT.

• Radiation Oncology Journal
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• v.38 no.2
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• pp.77-83
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• 2020

The management of rectal cancer is a major undertaking. There are currently multiple treatment modalities with variable degrees of complications. Radiotherapy (RT) is one of the more frequently used modalities either on its own or more frequently with chemotherapy mostly before the definitive surgery. The outcome of RT is unpredictable. RT has its serious side effects and there are no guarantees of its usefulness in all patients. This article outlines the effect of RT on the tumor, reviews the various staging systems of responses to RT and present recent evidence of which case is less responsive to such treatments to avoid unnecessary complications.

• Applied Chemistry for Engineering
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• v.17 no.2
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• pp.194-200
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• 2006

Titanium oxide films were deposited by the nozzle type HCP RT-MOCVD for the application of metal-oxide films. In the case of TTNB, after depositing films, films must be annealed at a proper temperature, but in the case of titanium ethoxide, titanium oxide films could be directly deposited by titanium ethoxide without general annealing. We could confirm that ratio of O to Ti in the films was about 2 : 1 at RF-power of 240 watt, distance between cathode and substrate of 3 cm, deposition time of 20 min, and ratio of Ar to $O_2$ of 1 : 1. Therefore, we could obtain the titanium oxide film deposited by the nozzle type HCP RT-MOCVD without an annealing process and could apply in the metal-oxide deposition process at a low temperature.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.651-654
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• 2008

In order to enhance the efficacy of norovirus detection by reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR, this study developed a norovirus mRNA concentration method using poly oligo dT-conjugated magnetic beads. An efficient norovirus detection protocol was performed on commercial ham using 2 viral elution buffers (glycine buffer and Tris beef extract buffer) and 2 concentration solutions [polyethylene glycol (PEG) and zirconium hydroxide]. The different approaches were verified by RT-PCR and nested PCR. This method was performed on ham in less than 8 hr by artificial inoculation of serial dilutions of the virus ranging from 1,000 to 1 RT-PCR unit/mL. The viral extraction and concentration method had 10-fold higher sensitivity using the combination of Tris beef extract buffer and PEG as compared to glycine buffer and zirconium hydroxide. This method proved that RT-PCR and nested PCR have the sensitive ability to detect norovirus in commercial ham, in that norovirus was successfully detected in artificially contaminated samples at a detection level as low as 1-10 RT-PCR unit/mL. Overall, such a detection limit suggests this protocol is both quick and efficient in terms of its potential use for detecting norovirus in meat products.

• Korean Journal of Pharmacognosy
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• v.44 no.2
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• pp.118-124
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• 2013

Mast cells is the key effector cells for IgE-mediated allergic responses. In this study, we investigated whether Rhus trichocarpa extract (RT) inhibited IgE-mediated allergic responses in mast cells and an allergic animal model. We further tried to find its mechanism of action in mast cells. We found that RT suppressed antigen-stimulated degranulation and production of TNF-${\alpha}$ and IL-4 in rat basophilic leukemia (RBL)-2H3 mast cells and bone marrow-derived mast cells (BMMC), as well as IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. As the mechanism of action of RT, it inhibited the activation of spleen tyrosine kinase (Syk), a pivotal signaling molecule for activation of mast cells and that of LAT, a downstream adaptor molecule of Syk in $Fc{\varepsilon}RI$-mediated signal pathways. RT also suppressed the activation of mitogen-activated protein (MAP) kinases and Akt. The current results demonstrated for the first time that RT has the anti-allergic effect through inhibiting degranulation and secretion of cytokines by suppression of Syk in antigen-stimulated mast cells. Therefore, RT might be useful for allergic diseases.